Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women
Human papillomavirus (HPV) is the main risk factor associated with the development of cervical cancer (CC); however, there are other factors, such as immunosuppression caused by the human immunodeficiency virus (HIV), that favor progression of the illness.This study was thus aimed at evaluating the...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2013
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/22302
- Acceso en línea:
- https://doi.org/10.1128/JCM.01302-13
https://repository.urosario.edu.co/handle/10336/22302
- Palabra clave:
- Virus DNA
Adult
Article
Cancer screening
Controlled study
Female
Human
Human immunodeficiency virus infection
Human papillomavirus type 11
Human papillomavirus type 16
Human papillomavirus type 18
Human papillomavirus type 31
Human papillomavirus type 33
Human papillomavirus type 45
Human papillomavirus type 58
Human papillomavirus type 6
Major clinical study
Mixed infection
Nonhuman
Papillomavirus infection
Polymerase chain reaction
Prevalence
Priority journal
Urinalysis
Uterine cervix cytology
Virus detection
Wart virus
Adult
Cervix Uteri
DNA Primers
Female
HIV Infections
Humans
Middle Aged
Molecular Diagnostic Techniques
Papillomaviridae
Papillomavirus Infections
Polymerase Chain Reaction
Sensitivity and Specificity
Urine
Virology
Viral
DNA
- Rights
- License
- Abierto (Texto Completo)
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5f0bfa81-3587-4e7d-9a77-20bc03da8208-1c67d367e-96c2-4566-8a39-374ead1a65f6-1c2710cd4-43c8-4225-9d8b-1d729ee41d9f-169762621-b985-4e26-8d05-2637a79c5e2f-10597c0e4-fd40-47e8-a426-80e3d809c8ad-15035c581-9072-482a-896b-18709bf3db92-110ecd4f9-843f-4ef2-bec0-7d39d3381a13-179653065-12020-05-25T23:56:02Z2020-05-25T23:56:02Z2013Human papillomavirus (HPV) is the main risk factor associated with the development of cervical cancer (CC); however, there are other factors, such as immunosuppression caused by the human immunodeficiency virus (HIV), that favor progression of the illness.This study was thus aimed at evaluating the functionality of classical PCR-based molecular tests for the generic identification of HPV DNA (GP5-/GP6-, MY09/MY11, and pU1M/2R primers, individually or in combination) using cervical and urine samples from 194 HIV-positive women.Infected samples were tested with type-specific primers for six high-risk types (HPV-16, -18, -31, -33, -45, and -58) and two low-risk types (HPV-6 and -11).HPV infection prevalence rates were 70.1% for the cervical samples and 63.9% for the urine samples.HPV-16 was the most prevalent viral type in the cervical and urine samples, with higher rates of multiple infections than single infections detected in such samples.HPV DNA detection by PCR (mainly with the pU1M/2R primer set) in rine samples was positively associated with abnormal cytological findings (atypical squamous cells of undetermined significance/squamous intraepithelial lesions [ASCUS/SIL]).It was determined that the operative characteristics for detection of cytological abnormalities were similar for cervical and urine samples.This suggested using PCR for the detection of HPV DNA in urine samples as a potential screening strategy for CC prevention in future prevention and control programs along with currently implemented strategies for reducing the impact of the disease, i.e., urine samples are economical, are easy to collect, have wide acceptability among women, and have operative characteristics similar to those of cervical samples.© 2013, American Society for Microbiology.All Rights Reserved.application/pdfhttps://doi.org/10.1128/JCM.01302-131098660X00951137https://repository.urosario.edu.co/handle/10336/22302eng3693No. 113688Journal of Clinical MicrobiologyVol. 51Journal of Clinical Microbiology, ISSN:1098660X, 00951137, Vol.51, No.11 (2013); pp. 3688-3693https://www.scopus.com/inward/record.uri?eid=2-s2.0-84886452411&doi=10.1128%2fJCM.01302-13&partnerID=40&md5=b937dc948296cbbbe847a2ad3e27ab74Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURVirus DNAAdultArticleCancer screeningControlled studyFemaleHumanHuman immunodeficiency virus infectionHuman papillomavirus type 11Human papillomavirus type 16Human papillomavirus type 18Human papillomavirus type 31Human papillomavirus type 33Human papillomavirus type 45Human papillomavirus type 58Human papillomavirus type 6Major clinical studyMixed infectionNonhumanPapillomavirus infectionPolymerase chain reactionPrevalencePriority journalUrinalysisUterine cervix cytologyVirus detectionWart virusAdultCervix UteriDNA PrimersFemaleHIV InfectionsHumansMiddle AgedMolecular Diagnostic TechniquesPapillomaviridaePapillomavirus InfectionsPolymerase Chain ReactionSensitivity and SpecificityUrineVirologyViralDNAClassical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected womenarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Munoz, MarinaCamargo, MilenaLeon, Sara C. Soto-DeSanchez, RicardoPineda-Peña, Andrea C.Perez-Prados, AntonioPatarroyo, Manuel E.Patarroyo, Manuel A.10336/22302oai:repository.urosario.edu.co:10336/223022022-05-02 07:37:13.985731https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |
dc.title.spa.fl_str_mv |
Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women |
title |
Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women |
spellingShingle |
Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women Virus DNA Adult Article Cancer screening Controlled study Female Human Human immunodeficiency virus infection Human papillomavirus type 11 Human papillomavirus type 16 Human papillomavirus type 18 Human papillomavirus type 31 Human papillomavirus type 33 Human papillomavirus type 45 Human papillomavirus type 58 Human papillomavirus type 6 Major clinical study Mixed infection Nonhuman Papillomavirus infection Polymerase chain reaction Prevalence Priority journal Urinalysis Uterine cervix cytology Virus detection Wart virus Adult Cervix Uteri DNA Primers Female HIV Infections Humans Middle Aged Molecular Diagnostic Techniques Papillomaviridae Papillomavirus Infections Polymerase Chain Reaction Sensitivity and Specificity Urine Virology Viral DNA |
title_short |
Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women |
title_full |
Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women |
title_fullStr |
Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women |
title_full_unstemmed |
Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women |
title_sort |
Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women |
dc.subject.keyword.spa.fl_str_mv |
Virus DNA Adult Article Cancer screening Controlled study Female Human Human immunodeficiency virus infection Human papillomavirus type 11 Human papillomavirus type 16 Human papillomavirus type 18 Human papillomavirus type 31 Human papillomavirus type 33 Human papillomavirus type 45 Human papillomavirus type 58 Human papillomavirus type 6 Major clinical study Mixed infection Nonhuman Papillomavirus infection Polymerase chain reaction Prevalence Priority journal Urinalysis Uterine cervix cytology Virus detection Wart virus Adult Cervix Uteri DNA Primers Female HIV Infections Humans Middle Aged Molecular Diagnostic Techniques Papillomaviridae Papillomavirus Infections Polymerase Chain Reaction Sensitivity and Specificity Urine Virology |
topic |
Virus DNA Adult Article Cancer screening Controlled study Female Human Human immunodeficiency virus infection Human papillomavirus type 11 Human papillomavirus type 16 Human papillomavirus type 18 Human papillomavirus type 31 Human papillomavirus type 33 Human papillomavirus type 45 Human papillomavirus type 58 Human papillomavirus type 6 Major clinical study Mixed infection Nonhuman Papillomavirus infection Polymerase chain reaction Prevalence Priority journal Urinalysis Uterine cervix cytology Virus detection Wart virus Adult Cervix Uteri DNA Primers Female HIV Infections Humans Middle Aged Molecular Diagnostic Techniques Papillomaviridae Papillomavirus Infections Polymerase Chain Reaction Sensitivity and Specificity Urine Virology Viral DNA |
dc.subject.keyword.eng.fl_str_mv |
Viral DNA |
description |
Human papillomavirus (HPV) is the main risk factor associated with the development of cervical cancer (CC); however, there are other factors, such as immunosuppression caused by the human immunodeficiency virus (HIV), that favor progression of the illness.This study was thus aimed at evaluating the functionality of classical PCR-based molecular tests for the generic identification of HPV DNA (GP5-/GP6-, MY09/MY11, and pU1M/2R primers, individually or in combination) using cervical and urine samples from 194 HIV-positive women.Infected samples were tested with type-specific primers for six high-risk types (HPV-16, -18, -31, -33, -45, and -58) and two low-risk types (HPV-6 and -11).HPV infection prevalence rates were 70.1% for the cervical samples and 63.9% for the urine samples.HPV-16 was the most prevalent viral type in the cervical and urine samples, with higher rates of multiple infections than single infections detected in such samples.HPV DNA detection by PCR (mainly with the pU1M/2R primer set) in rine samples was positively associated with abnormal cytological findings (atypical squamous cells of undetermined significance/squamous intraepithelial lesions [ASCUS/SIL]).It was determined that the operative characteristics for detection of cytological abnormalities were similar for cervical and urine samples.This suggested using PCR for the detection of HPV DNA in urine samples as a potential screening strategy for CC prevention in future prevention and control programs along with currently implemented strategies for reducing the impact of the disease, i.e., urine samples are economical, are easy to collect, have wide acceptability among women, and have operative characteristics similar to those of cervical samples.© 2013, American Society for Microbiology.All Rights Reserved. |
publishDate |
2013 |
dc.date.created.spa.fl_str_mv |
2013 |
dc.date.accessioned.none.fl_str_mv |
2020-05-25T23:56:02Z |
dc.date.available.none.fl_str_mv |
2020-05-25T23:56:02Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1128/JCM.01302-13 |
dc.identifier.issn.none.fl_str_mv |
1098660X 00951137 |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/22302 |
url |
https://doi.org/10.1128/JCM.01302-13 https://repository.urosario.edu.co/handle/10336/22302 |
identifier_str_mv |
1098660X 00951137 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.citationEndPage.none.fl_str_mv |
3693 |
dc.relation.citationIssue.none.fl_str_mv |
No. 11 |
dc.relation.citationStartPage.none.fl_str_mv |
3688 |
dc.relation.citationTitle.none.fl_str_mv |
Journal of Clinical Microbiology |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 51 |
dc.relation.ispartof.spa.fl_str_mv |
Journal of Clinical Microbiology, ISSN:1098660X, 00951137, Vol.51, No.11 (2013); pp. 3688-3693 |
dc.relation.uri.spa.fl_str_mv |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84886452411&doi=10.1128%2fJCM.01302-13&partnerID=40&md5=b937dc948296cbbbe847a2ad3e27ab74 |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.acceso.spa.fl_str_mv |
Abierto (Texto Completo) |
rights_invalid_str_mv |
Abierto (Texto Completo) http://purl.org/coar/access_right/c_abf2 |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
institution |
Universidad del Rosario |
dc.source.instname.spa.fl_str_mv |
instname:Universidad del Rosario |
dc.source.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional EdocUR |
repository.name.fl_str_mv |
Repositorio institucional EdocUR |
repository.mail.fl_str_mv |
edocur@urosario.edu.co |
_version_ |
1814167464458584064 |