Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women

Human papillomavirus (HPV) is the main risk factor associated with the development of cervical cancer (CC); however, there are other factors, such as immunosuppression caused by the human immunodeficiency virus (HIV), that favor progression of the illness.This study was thus aimed at evaluating the...

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Autores:
Tipo de recurso:
Fecha de publicación:
2013
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/22302
Acceso en línea:
https://doi.org/10.1128/JCM.01302-13
https://repository.urosario.edu.co/handle/10336/22302
Palabra clave:
Virus DNA
Adult
Article
Cancer screening
Controlled study
Female
Human
Human immunodeficiency virus infection
Human papillomavirus type 11
Human papillomavirus type 16
Human papillomavirus type 18
Human papillomavirus type 31
Human papillomavirus type 33
Human papillomavirus type 45
Human papillomavirus type 58
Human papillomavirus type 6
Major clinical study
Mixed infection
Nonhuman
Papillomavirus infection
Polymerase chain reaction
Prevalence
Priority journal
Urinalysis
Uterine cervix cytology
Virus detection
Wart virus
Adult
Cervix Uteri
DNA Primers
Female
HIV Infections
Humans
Middle Aged
Molecular Diagnostic Techniques
Papillomaviridae
Papillomavirus Infections
Polymerase Chain Reaction
Sensitivity and Specificity
Urine
Virology
Viral
DNA
Rights
License
Abierto (Texto Completo)
id EDOCUR2_953e8ca2e457af6756a7d2f7e7fe4958
oai_identifier_str oai:repository.urosario.edu.co:10336/22302
network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
spelling 5f0bfa81-3587-4e7d-9a77-20bc03da8208-1c67d367e-96c2-4566-8a39-374ead1a65f6-1c2710cd4-43c8-4225-9d8b-1d729ee41d9f-169762621-b985-4e26-8d05-2637a79c5e2f-10597c0e4-fd40-47e8-a426-80e3d809c8ad-15035c581-9072-482a-896b-18709bf3db92-110ecd4f9-843f-4ef2-bec0-7d39d3381a13-179653065-12020-05-25T23:56:02Z2020-05-25T23:56:02Z2013Human papillomavirus (HPV) is the main risk factor associated with the development of cervical cancer (CC); however, there are other factors, such as immunosuppression caused by the human immunodeficiency virus (HIV), that favor progression of the illness.This study was thus aimed at evaluating the functionality of classical PCR-based molecular tests for the generic identification of HPV DNA (GP5-/GP6-, MY09/MY11, and pU1M/2R primers, individually or in combination) using cervical and urine samples from 194 HIV-positive women.Infected samples were tested with type-specific primers for six high-risk types (HPV-16, -18, -31, -33, -45, and -58) and two low-risk types (HPV-6 and -11).HPV infection prevalence rates were 70.1% for the cervical samples and 63.9% for the urine samples.HPV-16 was the most prevalent viral type in the cervical and urine samples, with higher rates of multiple infections than single infections detected in such samples.HPV DNA detection by PCR (mainly with the pU1M/2R primer set) in rine samples was positively associated with abnormal cytological findings (atypical squamous cells of undetermined significance/squamous intraepithelial lesions [ASCUS/SIL]).It was determined that the operative characteristics for detection of cytological abnormalities were similar for cervical and urine samples.This suggested using PCR for the detection of HPV DNA in urine samples as a potential screening strategy for CC prevention in future prevention and control programs along with currently implemented strategies for reducing the impact of the disease, i.e., urine samples are economical, are easy to collect, have wide acceptability among women, and have operative characteristics similar to those of cervical samples.© 2013, American Society for Microbiology.All Rights Reserved.application/pdfhttps://doi.org/10.1128/JCM.01302-131098660X00951137https://repository.urosario.edu.co/handle/10336/22302eng3693No. 113688Journal of Clinical MicrobiologyVol. 51Journal of Clinical Microbiology, ISSN:1098660X, 00951137, Vol.51, No.11 (2013); pp. 3688-3693https://www.scopus.com/inward/record.uri?eid=2-s2.0-84886452411&doi=10.1128%2fJCM.01302-13&partnerID=40&md5=b937dc948296cbbbe847a2ad3e27ab74Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURVirus DNAAdultArticleCancer screeningControlled studyFemaleHumanHuman immunodeficiency virus infectionHuman papillomavirus type 11Human papillomavirus type 16Human papillomavirus type 18Human papillomavirus type 31Human papillomavirus type 33Human papillomavirus type 45Human papillomavirus type 58Human papillomavirus type 6Major clinical studyMixed infectionNonhumanPapillomavirus infectionPolymerase chain reactionPrevalencePriority journalUrinalysisUterine cervix cytologyVirus detectionWart virusAdultCervix UteriDNA PrimersFemaleHIV InfectionsHumansMiddle AgedMolecular Diagnostic TechniquesPapillomaviridaePapillomavirus InfectionsPolymerase Chain ReactionSensitivity and SpecificityUrineVirologyViralDNAClassical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected womenarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Munoz, MarinaCamargo, MilenaLeon, Sara C. Soto-DeSanchez, RicardoPineda-Peña, Andrea C.Perez-Prados, AntonioPatarroyo, Manuel E.Patarroyo, Manuel A.10336/22302oai:repository.urosario.edu.co:10336/223022022-05-02 07:37:13.985731https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women
title Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women
spellingShingle Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women
Virus DNA
Adult
Article
Cancer screening
Controlled study
Female
Human
Human immunodeficiency virus infection
Human papillomavirus type 11
Human papillomavirus type 16
Human papillomavirus type 18
Human papillomavirus type 31
Human papillomavirus type 33
Human papillomavirus type 45
Human papillomavirus type 58
Human papillomavirus type 6
Major clinical study
Mixed infection
Nonhuman
Papillomavirus infection
Polymerase chain reaction
Prevalence
Priority journal
Urinalysis
Uterine cervix cytology
Virus detection
Wart virus
Adult
Cervix Uteri
DNA Primers
Female
HIV Infections
Humans
Middle Aged
Molecular Diagnostic Techniques
Papillomaviridae
Papillomavirus Infections
Polymerase Chain Reaction
Sensitivity and Specificity
Urine
Virology
Viral
DNA
title_short Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women
title_full Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women
title_fullStr Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women
title_full_unstemmed Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women
title_sort Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women
dc.subject.keyword.spa.fl_str_mv Virus DNA
Adult
Article
Cancer screening
Controlled study
Female
Human
Human immunodeficiency virus infection
Human papillomavirus type 11
Human papillomavirus type 16
Human papillomavirus type 18
Human papillomavirus type 31
Human papillomavirus type 33
Human papillomavirus type 45
Human papillomavirus type 58
Human papillomavirus type 6
Major clinical study
Mixed infection
Nonhuman
Papillomavirus infection
Polymerase chain reaction
Prevalence
Priority journal
Urinalysis
Uterine cervix cytology
Virus detection
Wart virus
Adult
Cervix Uteri
DNA Primers
Female
HIV Infections
Humans
Middle Aged
Molecular Diagnostic Techniques
Papillomaviridae
Papillomavirus Infections
Polymerase Chain Reaction
Sensitivity and Specificity
Urine
Virology
topic Virus DNA
Adult
Article
Cancer screening
Controlled study
Female
Human
Human immunodeficiency virus infection
Human papillomavirus type 11
Human papillomavirus type 16
Human papillomavirus type 18
Human papillomavirus type 31
Human papillomavirus type 33
Human papillomavirus type 45
Human papillomavirus type 58
Human papillomavirus type 6
Major clinical study
Mixed infection
Nonhuman
Papillomavirus infection
Polymerase chain reaction
Prevalence
Priority journal
Urinalysis
Uterine cervix cytology
Virus detection
Wart virus
Adult
Cervix Uteri
DNA Primers
Female
HIV Infections
Humans
Middle Aged
Molecular Diagnostic Techniques
Papillomaviridae
Papillomavirus Infections
Polymerase Chain Reaction
Sensitivity and Specificity
Urine
Virology
Viral
DNA
dc.subject.keyword.eng.fl_str_mv Viral
DNA
description Human papillomavirus (HPV) is the main risk factor associated with the development of cervical cancer (CC); however, there are other factors, such as immunosuppression caused by the human immunodeficiency virus (HIV), that favor progression of the illness.This study was thus aimed at evaluating the functionality of classical PCR-based molecular tests for the generic identification of HPV DNA (GP5-/GP6-, MY09/MY11, and pU1M/2R primers, individually or in combination) using cervical and urine samples from 194 HIV-positive women.Infected samples were tested with type-specific primers for six high-risk types (HPV-16, -18, -31, -33, -45, and -58) and two low-risk types (HPV-6 and -11).HPV infection prevalence rates were 70.1% for the cervical samples and 63.9% for the urine samples.HPV-16 was the most prevalent viral type in the cervical and urine samples, with higher rates of multiple infections than single infections detected in such samples.HPV DNA detection by PCR (mainly with the pU1M/2R primer set) in rine samples was positively associated with abnormal cytological findings (atypical squamous cells of undetermined significance/squamous intraepithelial lesions [ASCUS/SIL]).It was determined that the operative characteristics for detection of cytological abnormalities were similar for cervical and urine samples.This suggested using PCR for the detection of HPV DNA in urine samples as a potential screening strategy for CC prevention in future prevention and control programs along with currently implemented strategies for reducing the impact of the disease, i.e., urine samples are economical, are easy to collect, have wide acceptability among women, and have operative characteristics similar to those of cervical samples.© 2013, American Society for Microbiology.All Rights Reserved.
publishDate 2013
dc.date.created.spa.fl_str_mv 2013
dc.date.accessioned.none.fl_str_mv 2020-05-25T23:56:02Z
dc.date.available.none.fl_str_mv 2020-05-25T23:56:02Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1128/JCM.01302-13
dc.identifier.issn.none.fl_str_mv 1098660X
00951137
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/22302
url https://doi.org/10.1128/JCM.01302-13
https://repository.urosario.edu.co/handle/10336/22302
identifier_str_mv 1098660X
00951137
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 3693
dc.relation.citationIssue.none.fl_str_mv No. 11
dc.relation.citationStartPage.none.fl_str_mv 3688
dc.relation.citationTitle.none.fl_str_mv Journal of Clinical Microbiology
dc.relation.citationVolume.none.fl_str_mv Vol. 51
dc.relation.ispartof.spa.fl_str_mv Journal of Clinical Microbiology, ISSN:1098660X, 00951137, Vol.51, No.11 (2013); pp. 3688-3693
dc.relation.uri.spa.fl_str_mv https://www.scopus.com/inward/record.uri?eid=2-s2.0-84886452411&doi=10.1128%2fJCM.01302-13&partnerID=40&md5=b937dc948296cbbbe847a2ad3e27ab74
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
rights_invalid_str_mv Abierto (Texto Completo)
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
institution Universidad del Rosario
dc.source.instname.spa.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.spa.fl_str_mv reponame:Repositorio Institucional EdocUR
repository.name.fl_str_mv Repositorio institucional EdocUR
repository.mail.fl_str_mv edocur@urosario.edu.co
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