Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids
Hybrid AD strains of the human pathogenic Cryptococcus neoformans species complex have been reported from many parts of the world. However, their origin, diversity, and evolution are incompletely understood. In this study, we analyzed 102 AD hybrid strains representing 21 countries on five continent...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2023
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/42150
- Acceso en línea:
- https://repository.urosario.edu.co/handle/10336/42150
- Palabra clave:
- M. Cogliati
P.E. Chidebelu
M. Hitchcock
M. Chen
V. Rickerts
S. Ackermann
M. Desnos Ollivier
J. Inácio
U. Nawrot
M. Florek
K.J. Kwon-Chung j
D.-H. Yang j
C. Firacative
C.A. Puime
P. Escandon
S. Bertout
F. Roger
J. Xu
- Rights
- License
- Attribution 4.0 International
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528186962024-01-31T18:31:09Z2024-01-31T18:31:09Z2023-12-192023Hybrid AD strains of the human pathogenic Cryptococcus neoformans species complex have been reported from many parts of the world. However, their origin, diversity, and evolution are incompletely understood. In this study, we analyzed 102 AD hybrid strains representing 21 countries on five continents. For each strain, we obtained its mating type and its allelic sequences at each of the seven loci that have been used for genotyping haploid serotypes A and D strains of the species complex by the Cryptococcus research community. Our results showed that most AD hybrids exhibited loss of heterozygosity at one or more of the seven analyzed loci. Phylogenetic and population genetic analyses of the allelic sequences revealed multiple origins of the hybrids within each continent, dating back to one million years ago in Africa and up to the present in other continents. We found evidence for clonal reproduction and long-distance dispersal of these hybrids in nature. Comparisons with the global haploid serotypes A and D strains identified new alleles and new haploid multi-locus genotypes in AD hybrids, consistent with the presence of yet-to-be discovered genetic diversity in haploid populations of this species complex in nature. Together, our results indicate that AD hybrids can be effectively genotyped using the same multi-locus sequencing type approach as that established for serotypes A and D strains. Our comparisons of the AD hybrids among each other as well as with the global haploid serotypes A and D strains revealed novel genetic diversity as well as evidence for multiple origins and dynamic evolution of these hybrids in nature.application/pdf10.1016/j.fgb.2023.1038611087-1845https://repository.urosario.edu.co/handle/10336/42150engUniversidad del Rosariohttps://www.sciencedirect.com/science/article/pii/S1087184523000920/pdfft?md5=95f92c11deffa51bb10ea69034028776&pid=1-s2.0-S1087184523000920-main.pdfAttribution 4.0 InternationalAbierto (Texto Completo)https://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2Fungal Genetics and Biologyinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURM. CogliatiP.E. ChidebeluM. HitchcockM. ChenV. RickertsS. AckermannM. Desnos OllivierJ. InácioU. NawrotM. FlorekK.J. Kwon-Chung jD.-H. Yang jC. FiracativeC.A. PuimeP. EscandonS. BertoutF. RogerJ. XuMulti-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybridsarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Firacative Ropero, Sandra CarolinaORIGINALMulti_locus_sequence_typing_phylogenetics.pdfapplication/pdf6481719https://repository.urosario.edu.co/bitstreams/7a893e94-9f71-4d47-9d91-db251058014f/downloada1b473fbecc4f6c002a433781aec35eaMD51TEXTMulti_locus_sequence_typing_phylogenetics.pdf.txtMulti_locus_sequence_typing_phylogenetics.pdf.txtExtracted texttext/plain62711https://repository.urosario.edu.co/bitstreams/30359d47-9acc-452a-8b07-5d6f19e376a1/download0c801fccbf471a763b2ff373d4ff9dc4MD52THUMBNAILMulti_locus_sequence_typing_phylogenetics.pdf.jpgMulti_locus_sequence_typing_phylogenetics.pdf.jpgGenerated Thumbnailimage/jpeg4407https://repository.urosario.edu.co/bitstreams/2ad03c09-e797-4196-8270-a46e30250c43/downloade093d99c3dbf18d18f4deb12c28cf291MD5310336/42150oai:repository.urosario.edu.co:10336/421502024-02-01 03:02:22.048https://creativecommons.org/licenses/by/4.0/Attribution 4.0 Internationalhttps://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |
dc.title.spa.fl_str_mv |
Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids |
title |
Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids |
spellingShingle |
Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids M. Cogliati P.E. Chidebelu M. Hitchcock M. Chen V. Rickerts S. Ackermann M. Desnos Ollivier J. Inácio U. Nawrot M. Florek K.J. Kwon-Chung j D.-H. Yang j C. Firacative C.A. Puime P. Escandon S. Bertout F. Roger J. Xu |
title_short |
Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids |
title_full |
Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids |
title_fullStr |
Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids |
title_full_unstemmed |
Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids |
title_sort |
Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids |
dc.subject.spa.fl_str_mv |
M. Cogliati P.E. Chidebelu M. Hitchcock M. Chen V. Rickerts S. Ackermann M. Desnos Ollivier J. Inácio U. Nawrot M. Florek K.J. Kwon-Chung j D.-H. Yang j C. Firacative C.A. Puime P. Escandon S. Bertout F. Roger J. Xu |
topic |
M. Cogliati P.E. Chidebelu M. Hitchcock M. Chen V. Rickerts S. Ackermann M. Desnos Ollivier J. Inácio U. Nawrot M. Florek K.J. Kwon-Chung j D.-H. Yang j C. Firacative C.A. Puime P. Escandon S. Bertout F. Roger J. Xu |
description |
Hybrid AD strains of the human pathogenic Cryptococcus neoformans species complex have been reported from many parts of the world. However, their origin, diversity, and evolution are incompletely understood. In this study, we analyzed 102 AD hybrid strains representing 21 countries on five continents. For each strain, we obtained its mating type and its allelic sequences at each of the seven loci that have been used for genotyping haploid serotypes A and D strains of the species complex by the Cryptococcus research community. Our results showed that most AD hybrids exhibited loss of heterozygosity at one or more of the seven analyzed loci. Phylogenetic and population genetic analyses of the allelic sequences revealed multiple origins of the hybrids within each continent, dating back to one million years ago in Africa and up to the present in other continents. We found evidence for clonal reproduction and long-distance dispersal of these hybrids in nature. Comparisons with the global haploid serotypes A and D strains identified new alleles and new haploid multi-locus genotypes in AD hybrids, consistent with the presence of yet-to-be discovered genetic diversity in haploid populations of this species complex in nature. Together, our results indicate that AD hybrids can be effectively genotyped using the same multi-locus sequencing type approach as that established for serotypes A and D strains. Our comparisons of the AD hybrids among each other as well as with the global haploid serotypes A and D strains revealed novel genetic diversity as well as evidence for multiple origins and dynamic evolution of these hybrids in nature. |
publishDate |
2023 |
dc.date.created.spa.fl_str_mv |
2023-12-19 |
dc.date.issued.spa.fl_str_mv |
2023 |
dc.date.accessioned.none.fl_str_mv |
2024-01-31T18:31:09Z |
dc.date.available.none.fl_str_mv |
2024-01-31T18:31:09Z |
dc.type.spa.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.spa.fl_str_mv |
10.1016/j.fgb.2023.103861 |
dc.identifier.issn.spa.fl_str_mv |
1087-1845 |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/42150 |
identifier_str_mv |
10.1016/j.fgb.2023.103861 1087-1845 |
url |
https://repository.urosario.edu.co/handle/10336/42150 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.uri.spa.fl_str_mv |
https://www.sciencedirect.com/science/article/pii/S1087184523000920/pdfft?md5=95f92c11deffa51bb10ea69034028776&pid=1-s2.0-S1087184523000920-main.pdf |
dc.rights.spa.fl_str_mv |
Attribution 4.0 International |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.acceso.spa.fl_str_mv |
Abierto (Texto Completo) |
dc.rights.uri.spa.fl_str_mv |
https://creativecommons.org/licenses/by/4.0/ |
rights_invalid_str_mv |
Attribution 4.0 International Abierto (Texto Completo) https://creativecommons.org/licenses/by/4.0/ http://purl.org/coar/access_right/c_abf2 |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Universidad del Rosario |
dc.source.spa.fl_str_mv |
Fungal Genetics and Biology |
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Universidad del Rosario |
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