Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids

Hybrid AD strains of the human pathogenic Cryptococcus neoformans species complex have been reported from many parts of the world. However, their origin, diversity, and evolution are incompletely understood. In this study, we analyzed 102 AD hybrid strains representing 21 countries on five continent...

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Fecha de publicación:
2023
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/42150
Acceso en línea:
https://repository.urosario.edu.co/handle/10336/42150
Palabra clave:
M. Cogliati
P.E. Chidebelu
M. Hitchcock
M. Chen
V. Rickerts
S. Ackermann
M. Desnos Ollivier
J. Inácio
U. Nawrot
M. Florek
K.J. Kwon-Chung j
D.-H. Yang j
C. Firacative
C.A. Puime
P. Escandon
S. Bertout
F. Roger
J. Xu
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Attribution 4.0 International
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spelling 528186962024-01-31T18:31:09Z2024-01-31T18:31:09Z2023-12-192023Hybrid AD strains of the human pathogenic Cryptococcus neoformans species complex have been reported from many parts of the world. However, their origin, diversity, and evolution are incompletely understood. In this study, we analyzed 102 AD hybrid strains representing 21 countries on five continents. For each strain, we obtained its mating type and its allelic sequences at each of the seven loci that have been used for genotyping haploid serotypes A and D strains of the species complex by the Cryptococcus research community. Our results showed that most AD hybrids exhibited loss of heterozygosity at one or more of the seven analyzed loci. Phylogenetic and population genetic analyses of the allelic sequences revealed multiple origins of the hybrids within each continent, dating back to one million years ago in Africa and up to the present in other continents. We found evidence for clonal reproduction and long-distance dispersal of these hybrids in nature. Comparisons with the global haploid serotypes A and D strains identified new alleles and new haploid multi-locus genotypes in AD hybrids, consistent with the presence of yet-to-be discovered genetic diversity in haploid populations of this species complex in nature. Together, our results indicate that AD hybrids can be effectively genotyped using the same multi-locus sequencing type approach as that established for serotypes A and D strains. Our comparisons of the AD hybrids among each other as well as with the global haploid serotypes A and D strains revealed novel genetic diversity as well as evidence for multiple origins and dynamic evolution of these hybrids in nature.application/pdf10.1016/j.fgb.2023.1038611087-1845https://repository.urosario.edu.co/handle/10336/42150engUniversidad del Rosariohttps://www.sciencedirect.com/science/article/pii/S1087184523000920/pdfft?md5=95f92c11deffa51bb10ea69034028776&pid=1-s2.0-S1087184523000920-main.pdfAttribution 4.0 InternationalAbierto (Texto Completo)https://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2Fungal Genetics and Biologyinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURM. CogliatiP.E. ChidebeluM. HitchcockM. ChenV. RickertsS. AckermannM. Desnos OllivierJ. InácioU. NawrotM. FlorekK.J. Kwon-Chung jD.-H. Yang jC. FiracativeC.A. PuimeP. EscandonS. BertoutF. RogerJ. XuMulti-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybridsarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Firacative Ropero, Sandra CarolinaORIGINALMulti_locus_sequence_typing_phylogenetics.pdfapplication/pdf6481719https://repository.urosario.edu.co/bitstreams/7a893e94-9f71-4d47-9d91-db251058014f/downloada1b473fbecc4f6c002a433781aec35eaMD51TEXTMulti_locus_sequence_typing_phylogenetics.pdf.txtMulti_locus_sequence_typing_phylogenetics.pdf.txtExtracted texttext/plain62711https://repository.urosario.edu.co/bitstreams/30359d47-9acc-452a-8b07-5d6f19e376a1/download0c801fccbf471a763b2ff373d4ff9dc4MD52THUMBNAILMulti_locus_sequence_typing_phylogenetics.pdf.jpgMulti_locus_sequence_typing_phylogenetics.pdf.jpgGenerated Thumbnailimage/jpeg4407https://repository.urosario.edu.co/bitstreams/2ad03c09-e797-4196-8270-a46e30250c43/downloade093d99c3dbf18d18f4deb12c28cf291MD5310336/42150oai:repository.urosario.edu.co:10336/421502024-02-01 03:02:22.048https://creativecommons.org/licenses/by/4.0/Attribution 4.0 Internationalhttps://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids
title Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids
spellingShingle Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids
M. Cogliati
P.E. Chidebelu
M. Hitchcock
M. Chen
V. Rickerts
S. Ackermann
M. Desnos Ollivier
J. Inácio
U. Nawrot
M. Florek
K.J. Kwon-Chung j
D.-H. Yang j
C. Firacative
C.A. Puime
P. Escandon
S. Bertout
F. Roger
J. Xu
title_short Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids
title_full Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids
title_fullStr Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids
title_full_unstemmed Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids
title_sort Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids
dc.subject.spa.fl_str_mv M. Cogliati
P.E. Chidebelu
M. Hitchcock
M. Chen
V. Rickerts
S. Ackermann
M. Desnos Ollivier
J. Inácio
U. Nawrot
M. Florek
K.J. Kwon-Chung j
D.-H. Yang j
C. Firacative
C.A. Puime
P. Escandon
S. Bertout
F. Roger
J. Xu
topic M. Cogliati
P.E. Chidebelu
M. Hitchcock
M. Chen
V. Rickerts
S. Ackermann
M. Desnos Ollivier
J. Inácio
U. Nawrot
M. Florek
K.J. Kwon-Chung j
D.-H. Yang j
C. Firacative
C.A. Puime
P. Escandon
S. Bertout
F. Roger
J. Xu
description Hybrid AD strains of the human pathogenic Cryptococcus neoformans species complex have been reported from many parts of the world. However, their origin, diversity, and evolution are incompletely understood. In this study, we analyzed 102 AD hybrid strains representing 21 countries on five continents. For each strain, we obtained its mating type and its allelic sequences at each of the seven loci that have been used for genotyping haploid serotypes A and D strains of the species complex by the Cryptococcus research community. Our results showed that most AD hybrids exhibited loss of heterozygosity at one or more of the seven analyzed loci. Phylogenetic and population genetic analyses of the allelic sequences revealed multiple origins of the hybrids within each continent, dating back to one million years ago in Africa and up to the present in other continents. We found evidence for clonal reproduction and long-distance dispersal of these hybrids in nature. Comparisons with the global haploid serotypes A and D strains identified new alleles and new haploid multi-locus genotypes in AD hybrids, consistent with the presence of yet-to-be discovered genetic diversity in haploid populations of this species complex in nature. Together, our results indicate that AD hybrids can be effectively genotyped using the same multi-locus sequencing type approach as that established for serotypes A and D strains. Our comparisons of the AD hybrids among each other as well as with the global haploid serotypes A and D strains revealed novel genetic diversity as well as evidence for multiple origins and dynamic evolution of these hybrids in nature.
publishDate 2023
dc.date.created.spa.fl_str_mv 2023-12-19
dc.date.issued.spa.fl_str_mv 2023
dc.date.accessioned.none.fl_str_mv 2024-01-31T18:31:09Z
dc.date.available.none.fl_str_mv 2024-01-31T18:31:09Z
dc.type.spa.fl_str_mv article
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dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.spa.fl_str_mv 10.1016/j.fgb.2023.103861
dc.identifier.issn.spa.fl_str_mv 1087-1845
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/42150
identifier_str_mv 10.1016/j.fgb.2023.103861
1087-1845
url https://repository.urosario.edu.co/handle/10336/42150
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.uri.spa.fl_str_mv https://www.sciencedirect.com/science/article/pii/S1087184523000920/pdfft?md5=95f92c11deffa51bb10ea69034028776&pid=1-s2.0-S1087184523000920-main.pdf
dc.rights.spa.fl_str_mv Attribution 4.0 International
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
dc.rights.uri.spa.fl_str_mv https://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv Attribution 4.0 International
Abierto (Texto Completo)
https://creativecommons.org/licenses/by/4.0/
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad del Rosario
dc.source.spa.fl_str_mv Fungal Genetics and Biology
institution Universidad del Rosario
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