Patients with Primary Sjögren's Syndrome Who Are Positive for Autoantibodies to Tripartite Motif-Containing Protein 38 Show Greater Disease Severity
Objective Autoantibodies reactive with Ro52 (tripartite motif-containing protein 21 [TRIM21]) are detected in 70% of patients with primary Sjögren's syndrome (SS). TRIM21 belongs to a 34-member C-IV family of TRIM proteins. Although autoantibodies against other TRIM proteins within the C-IV fam...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2016
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/23011
- Acceso en línea:
- https://doi.org/10.1002/art.39497
https://repository.urosario.edu.co/handle/10336/23011
- Palabra clave:
- Autoantibody
Cortactin
Cortactin binding protein 2
La antibody
Membrane protein
Rheumatoid factor
Ro52 antibody
Ro60 antibody
Tripartite motif containing protein 21
Tripartite motif containing protein 38
Unclassified drug
Autoantibody
Carrier protein
Methionine
Rheumatoid factor
Ribonucleoprotein
Ss-a antigen
Sulfur
Adult
Antibody blood level
Antibody detection
Antibody specificity
Article
Controlled study
Disease severity
Female
Human
Hypergammaglobulinemia
Immunoprecipitation
Major clinical study
Male
Middle aged
Nonhuman
Priority journal
Protein domain
Protein family
Salivary gland biopsy
Schirmer test
Sjoegren syndrome
Blood
Immunology
Pathophysiology
Severity of illness index
Sjoegren syndrome
Autoantibodies
Carrier proteins
Female
Humans
Hypergammaglobulinemia
Immunoprecipitation
Male
Methionine
Middle aged
Rheumatoid factor
Ribonucleoproteins
Severity of illness index
Sjogren's syndrome
Sulfur radioisotopes
human
Trim38 protein
- Rights
- License
- Abierto (Texto Completo)
Summary: | Objective Autoantibodies reactive with Ro52 (tripartite motif-containing protein 21 [TRIM21]) are detected in 70% of patients with primary Sjögren's syndrome (SS). TRIM21 belongs to a 34-member C-IV family of TRIM proteins. Although autoantibodies against other TRIM proteins within the C-IV family have been detected in the sera of patients with primary SS, their clinical relevance remains unclear. This study was undertaken to investigate the frequency of anti-TRIM38 in patients with primary SS and evaluate its association with various clinical measures of the disease. Methods Serum samples from patients with primary SS (n = 235) and controls (n = 50) were analyzed for reactivity with in vitro-transcribed and -translated 35S-methionine-labeled TRIM38 protein. The associations of anti-TRIM38 with various laboratory and clinical measures of primary SS were evaluated. Reactivity of anti-TRIM38 with different structural domains of TRIM38 was analyzed. Affinity-purified anti-TRIM38 antibodies were used to immunoprecipitate TRIM21. Results TRIM38-reactive autoantibodies were detected in the sera of 24 of the 235 patients with primary SS and 2 of the 50 controls. Anti-TRIM38 positivity was significantly associated with the presence of anti-Ro60, anti-Ro52, anti-La, rheumatoid factor, and hypergammaglobulinemia. Clinically, anti-TRIM38 was associated with significantly higher ocular surface staining scores, lower Schirmer's test scores, and minor labial salivary gland biopsy focus scores of ?3.0. Anti-TRIM38 antibodies mainly recognized the cortactin-binding protein 2 (CortBP-2; amino acids 128-238) and the B30.2/SPRY (amino acids 268-465) domains on TRIM38. Affinity-purified antibodies to TRIM38-CortBP-2 and TRIM38-B30.2/SPRY domains reacted with TRIM21. Conclusion Our data demonstrate that anti-TRIM38 specificity arising in a subset of patients with primary SS is associated with increased severity of the disease. © 2016, American College of Rheumatology. |
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