Lulo cell line derived from Lutzomyia longipalpis (Diptera: Psychodidae) : A novel model to assay Leishmania spp. and vector interaction
Background: Leishmania (Vianna) braziliensis, Leishmania (Leishmania) amazonensis and Leishmania (Leishmania) chagasi are important parasites in the scenario of leishmaniasis in Brazil. During the life cycle of these parasites, the promastigote forms adhere to the midgut epithelial microvillii of ph...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2011
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/21894
- Acceso en línea:
- https://doi.org/10.1186/1756-3305-4-216
https://repository.urosario.edu.co/handle/10336/21894
- Palabra clave:
- Enfermedades
Microbiología
Leishmania spp
Lulo cell
Phlebotominae
Promastigotes
- Rights
- License
- Abierto (Texto Completo)
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dc.title.spa.fl_str_mv |
Lulo cell line derived from Lutzomyia longipalpis (Diptera: Psychodidae) : A novel model to assay Leishmania spp. and vector interaction |
title |
Lulo cell line derived from Lutzomyia longipalpis (Diptera: Psychodidae) : A novel model to assay Leishmania spp. and vector interaction |
spellingShingle |
Lulo cell line derived from Lutzomyia longipalpis (Diptera: Psychodidae) : A novel model to assay Leishmania spp. and vector interaction Enfermedades Microbiología Leishmania spp Lulo cell Phlebotominae Promastigotes |
title_short |
Lulo cell line derived from Lutzomyia longipalpis (Diptera: Psychodidae) : A novel model to assay Leishmania spp. and vector interaction |
title_full |
Lulo cell line derived from Lutzomyia longipalpis (Diptera: Psychodidae) : A novel model to assay Leishmania spp. and vector interaction |
title_fullStr |
Lulo cell line derived from Lutzomyia longipalpis (Diptera: Psychodidae) : A novel model to assay Leishmania spp. and vector interaction |
title_full_unstemmed |
Lulo cell line derived from Lutzomyia longipalpis (Diptera: Psychodidae) : A novel model to assay Leishmania spp. and vector interaction |
title_sort |
Lulo cell line derived from Lutzomyia longipalpis (Diptera: Psychodidae) : A novel model to assay Leishmania spp. and vector interaction |
dc.subject.ddc.spa.fl_str_mv |
Enfermedades Microbiología |
topic |
Enfermedades Microbiología Leishmania spp Lulo cell Phlebotominae Promastigotes |
dc.subject.keyword.spa.fl_str_mv |
Leishmania spp Lulo cell Phlebotominae Promastigotes |
description |
Background: Leishmania (Vianna) braziliensis, Leishmania (Leishmania) amazonensis and Leishmania (Leishmania) chagasi are important parasites in the scenario of leishmaniasis in Brazil. During the life cycle of these parasites, the promastigote forms adhere to the midgut epithelial microvillii of phlebotomine insects to avoid being secreted along with digestive products. Lulo cells are a potential model that will help to understand the features of this adhesion phenomenon. Here, we analyze the interaction between Leishmania spp. promastigotes and Lulo cells in vitro, specifically focusing on adhesion events occurring between three Leishmania species and this cell line. Methods. Confluent monolayers of Lulo cells were incubated with promastigotes and adhesion was assessed using both light microscopy and scanning electron microscopy. Findings. The results indicate that species from the subgenera Leishmania and Viannia have great potential to adhere to Lulo cells. The highest adherence rate was observed for L. (L.) chagasi after 24 h of incubation with Lulo cells (27.3 1.8% of cells with adhered promastigotes), followed by L. (L.) amazonensis (16.0 0.7%) and L. (V.) braziliensis (3.0 0.7%), both after 48 h. In the ultrastructural analysis, promastigote adherence was also assessed by scanning electron microscopy, showing that, for parasites from both subgenera, adhesion occurs by both the body and the flagellum. The interaction of Lulo cells with Leishmania (L.) chagasi showed the participation of cytoplasmic projections from the former closely associating the parasites with the cells. Conclusions: We present evidence that Lulo cells can be useful in studies of insect-parasite interactions for Leishmania species. © 2011 Côrtes et al; licensee BioMed Central Ltd. |
publishDate |
2011 |
dc.date.created.none.fl_str_mv |
2011 |
dc.date.issued.none.fl_str_mv |
2011 |
dc.date.accessioned.none.fl_str_mv |
2020-05-07T14:45:54Z |
dc.date.available.none.fl_str_mv |
2020-05-07T14:45:54Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1186/1756-3305-4-216 |
dc.identifier.issn.none.fl_str_mv |
1756-3305 |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/21894 |
url |
https://doi.org/10.1186/1756-3305-4-216 https://repository.urosario.edu.co/handle/10336/21894 |
identifier_str_mv |
1756-3305 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.citationIssue.none.fl_str_mv |
No. 1 |
dc.relation.citationTitle.none.fl_str_mv |
Parasites and Vectors |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 4 |
dc.relation.ispartof.spa.fl_str_mv |
Parasites and Vectors, ISSN: 1756-3305 Vol. 4, No. 1 (2011) |
dc.relation.uri.spa.fl_str_mv |
https://parasitesandvectors.biomedcentral.com/articles/10.1186/1756-3305-4-216 |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.acceso.spa.fl_str_mv |
Abierto (Texto Completo) |
rights_invalid_str_mv |
Abierto (Texto Completo) http://purl.org/coar/access_right/c_abf2 |
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application/pdf |
institution |
Universidad del Rosario |
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reponame:Repositorio Institucional EdocUR |
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ea0bb9ab-b4df-44d1-90a4-0b0afc815e166000f76576d-eee2-4cff-9f78-6daa61cde46d60060daaae5-2cf9-4af3-8053-9142622935af6008f37d6c6-428a-49c0-9b8f-3ee78ef98f726001543b035-b6d6-4d30-b0ca-e6c411efa0dc600743756f9-9669-44b0-8a52-23524ddfcda26005b85917d-3ccf-44e4-8902-85626159374b6001307616b-eb77-46b6-a3db-d212242289bb6003ea9f908-6d1b-461e-9b6d-2575ba06e4c860087477967-cb27-4e86-9872-726f5e0f504160042319547-732e-41ee-a686-f88a8b329b836002020-05-07T14:45:54Z2020-05-07T14:45:54Z20112011Background: Leishmania (Vianna) braziliensis, Leishmania (Leishmania) amazonensis and Leishmania (Leishmania) chagasi are important parasites in the scenario of leishmaniasis in Brazil. During the life cycle of these parasites, the promastigote forms adhere to the midgut epithelial microvillii of phlebotomine insects to avoid being secreted along with digestive products. Lulo cells are a potential model that will help to understand the features of this adhesion phenomenon. Here, we analyze the interaction between Leishmania spp. promastigotes and Lulo cells in vitro, specifically focusing on adhesion events occurring between three Leishmania species and this cell line. Methods. Confluent monolayers of Lulo cells were incubated with promastigotes and adhesion was assessed using both light microscopy and scanning electron microscopy. Findings. The results indicate that species from the subgenera Leishmania and Viannia have great potential to adhere to Lulo cells. The highest adherence rate was observed for L. (L.) chagasi after 24 h of incubation with Lulo cells (27.3 1.8% of cells with adhered promastigotes), followed by L. (L.) amazonensis (16.0 0.7%) and L. (V.) braziliensis (3.0 0.7%), both after 48 h. In the ultrastructural analysis, promastigote adherence was also assessed by scanning electron microscopy, showing that, for parasites from both subgenera, adhesion occurs by both the body and the flagellum. The interaction of Lulo cells with Leishmania (L.) chagasi showed the participation of cytoplasmic projections from the former closely associating the parasites with the cells. Conclusions: We present evidence that Lulo cells can be useful in studies of insect-parasite interactions for Leishmania species. © 2011 Côrtes et al; licensee BioMed Central Ltd.application/pdfhttps://doi.org/10.1186/1756-3305-4-2161756-3305https://repository.urosario.edu.co/handle/10336/21894engNo. 1Parasites and VectorsVol. 4Parasites and Vectors, ISSN: 1756-3305 Vol. 4, No. 1 (2011)https://parasitesandvectors.biomedcentral.com/articles/10.1186/1756-3305-4-216Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocUREnfermedades616600Microbiología576600Leishmania sppLulo cellPhlebotominaePromastigotesLulo cell line derived from Lutzomyia longipalpis (Diptera: Psychodidae) : A novel model to assay Leishmania spp. and vector interactionarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Côrtes, Luzia MCSilva, Roger MMPereira, Bernardo ASGuerra, CamilaZapata, Angela CBello, Felio J.Finkelstein, Léa CMadeira, Maria FBrazil, Reginaldo PCôrte-Real, SuzanaAlves, Carlos RCôrtes, Luzia MCSilva, Roger MMPereira, Bernardo ASGuerra, CamilaZapata, Angela CBello, Felio JFinkelstein, Léa CMadeira, Maria FBrazil, Reginaldo PCôrte-Real, SuzanaAlves, Carlos RORIGINALLulo_cell_line_derived_from_Lutzomyia.pdfapplication/pdf2700185https://repository.urosario.edu.co/bitstreams/92447694-fff2-47fa-9bb9-4b36be6cc0e3/downloaddae1384b5e53e99bc9de9c3c2259833eMD51TEXTLulo_cell_line_derived_from_Lutzomyia.pdf.txtLulo_cell_line_derived_from_Lutzomyia.pdf.txtExtracted texttext/plain21263https://repository.urosario.edu.co/bitstreams/60482e91-9c2d-4e2c-822c-981f96b49e8f/downloadf0f631abf91271fb1710a2541e57b885MD52THUMBNAILLulo_cell_line_derived_from_Lutzomyia.pdf.jpgLulo_cell_line_derived_from_Lutzomyia.pdf.jpgGenerated Thumbnailimage/jpeg4654https://repository.urosario.edu.co/bitstreams/8f8cb823-51d3-4286-98ec-d5cdf59d34e4/downloadc0327cd15303a4c7bd80a79f851865b1MD5310336/21894oai:repository.urosario.edu.co:10336/218942020-05-13 14:48:20.109https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |