Analysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markers
Ochlerotatus taeniorhynchus (Diptera: Culicidae) is a mosquito, which is an efficient vector of the virus causing epidemic-epizootic Venezuelan equine encephalitis in Colombia. This study used 9 random amplified polymorphic DNA (RAPD) markers to analyze the mosquito's genetic variability and ge...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2014
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/24147
- Acceso en línea:
- https://doi.org/10.4238/2014.May.30.6
https://repository.urosario.edu.co/handle/10336/24147
- Palabra clave:
- Isoenzyme
Microsatellite dna
Article
Controlled study
Gene flow
Gene locus
Genetic distance
Genetic heterogeneity
Genetic variability
Genome analysis
Genome size
Genomic fragment
Heterozygosity
Insect genome
Microsatellite marker
Nonhuman
Nucleotide sequence
Ochlerotatus
Ochlerotatus taeniorhynchus
Population genetic structure
Random amplified polymorphic dna
Spatial autocorrelation analysis
Animal
Colombia
Genetic variability
Genetics
Heterozygote
Ochlerotatus
Population genetics
Animals
Colombia
Genetic variation
Heterozygote
Microsatellite repeats
Ochlerotatus
Random amplified polymorphic dna technique
Genetic heterogeneity
Genetic variability
Ochlerotatus taeniorhynchus
Random amplified polymorphic dna-polymerase chain reaction
Spatial autocorrelation
population
Genetics
- Rights
- License
- Abierto (Texto Completo)
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25f0b849-c4d2-4ab9-ab9c-281620017867-13deb198c-1188-4d28-aa55-78c0f442fb54-1bd3e4519-7b71-477d-a72c-750bdf74c360-12020-05-26T00:09:15Z2020-05-26T00:09:15Z2014Ochlerotatus taeniorhynchus (Diptera: Culicidae) is a mosquito, which is an efficient vector of the virus causing epidemic-epizootic Venezuelan equine encephalitis in Colombia. This study used 9 random amplified polymorphic DNA (RAPD) markers to analyze the mosquito's genetic variability and genetic structure of 122 specimens in 7 populations from the Colombian Atlantic coast. Assuming that all loci were in Hardy-Weinberg equilibrium, diversity statistics and analyses were performed. The average number of amplified fragments for each primer was 8.3, and the size of these fragments ranged from 350 to 3600 bp. The expected average heterozygosity was 0.358 ± 0.103. The genetic heterogeneity among the populations studied was small (GST = 0.05 ± 0.01); meanwhile, the gene flow estimates (Nm = 7.32 ± 1.35) were high. In an identical way, the Nei's genetic distances obtained yielded very small values amongst the populations that were studied in this Colombian region. Furthermore, a spatial autocorrelation analysis with Moran's I index revealed a very weak, or inexistent, spatial genetic structure among these populations. The comparison of these results with those performed for other markers (isoenzymes and microsatellites) in populations of this same mosquito from the Colombian Atlantic coast was discussed. The results of our RAPD analysis showed scarce genetic differentiation among the mosquito populations on the Colombian Atlantic coast, which was probably determined by high gene flow levels. © FUNPEC-RP.application/pdfhttps://doi.org/10.4238/2014.May.30.616765680https://repository.urosario.edu.co/handle/10336/24147engFundacao de Pesquisas Cientificas de Ribeirao Preto4123No. 24110Genetics and Molecular ResearchVol. 13Genetics and Molecular Research, ISSN:16765680, Vol.13, No.2 (2014); pp. 4110-4123https://www.scopus.com/inward/record.uri?eid=2-s2.0-84901767197&doi=10.4238%2f2014.May.30.6&partnerID=40&md5=551772c3a134daf5815fd1ec96501a77Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURIsoenzymeMicrosatellite dnaArticleControlled studyGene flowGene locusGenetic distanceGenetic heterogeneityGenetic variabilityGenome analysisGenome sizeGenomic fragmentHeterozygosityInsect genomeMicrosatellite markerNonhumanNucleotide sequenceOchlerotatusOchlerotatus taeniorhynchusPopulation genetic structureRandom amplified polymorphic dnaSpatial autocorrelation analysisAnimalColombiaGenetic variabilityGeneticsHeterozygoteOchlerotatusPopulation geneticsAnimalsColombiaGenetic variationHeterozygoteMicrosatellite repeatsOchlerotatusRandom amplified polymorphic dna techniqueGenetic heterogeneityGenetic variabilityOchlerotatus taeniorhynchusRandom amplified polymorphic dna-polymerase chain reactionSpatial autocorrelationpopulationGeneticsAnalysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markersarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Bello, F JSegura, N ARuiz-Garcia, M10336/24147oai:repository.urosario.edu.co:10336/241472022-05-02 07:37:14.932427https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |
dc.title.spa.fl_str_mv |
Analysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markers |
title |
Analysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markers |
spellingShingle |
Analysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markers Isoenzyme Microsatellite dna Article Controlled study Gene flow Gene locus Genetic distance Genetic heterogeneity Genetic variability Genome analysis Genome size Genomic fragment Heterozygosity Insect genome Microsatellite marker Nonhuman Nucleotide sequence Ochlerotatus Ochlerotatus taeniorhynchus Population genetic structure Random amplified polymorphic dna Spatial autocorrelation analysis Animal Colombia Genetic variability Genetics Heterozygote Ochlerotatus Population genetics Animals Colombia Genetic variation Heterozygote Microsatellite repeats Ochlerotatus Random amplified polymorphic dna technique Genetic heterogeneity Genetic variability Ochlerotatus taeniorhynchus Random amplified polymorphic dna-polymerase chain reaction Spatial autocorrelation population Genetics |
title_short |
Analysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markers |
title_full |
Analysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markers |
title_fullStr |
Analysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markers |
title_full_unstemmed |
Analysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markers |
title_sort |
Analysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markers |
dc.subject.keyword.spa.fl_str_mv |
Isoenzyme Microsatellite dna Article Controlled study Gene flow Gene locus Genetic distance Genetic heterogeneity Genetic variability Genome analysis Genome size Genomic fragment Heterozygosity Insect genome Microsatellite marker Nonhuman Nucleotide sequence Ochlerotatus Ochlerotatus taeniorhynchus Population genetic structure Random amplified polymorphic dna Spatial autocorrelation analysis Animal Colombia Genetic variability Genetics Heterozygote Ochlerotatus Population genetics Animals Colombia Genetic variation Heterozygote Microsatellite repeats Ochlerotatus Random amplified polymorphic dna technique Genetic heterogeneity Genetic variability Ochlerotatus taeniorhynchus Random amplified polymorphic dna-polymerase chain reaction Spatial autocorrelation |
topic |
Isoenzyme Microsatellite dna Article Controlled study Gene flow Gene locus Genetic distance Genetic heterogeneity Genetic variability Genome analysis Genome size Genomic fragment Heterozygosity Insect genome Microsatellite marker Nonhuman Nucleotide sequence Ochlerotatus Ochlerotatus taeniorhynchus Population genetic structure Random amplified polymorphic dna Spatial autocorrelation analysis Animal Colombia Genetic variability Genetics Heterozygote Ochlerotatus Population genetics Animals Colombia Genetic variation Heterozygote Microsatellite repeats Ochlerotatus Random amplified polymorphic dna technique Genetic heterogeneity Genetic variability Ochlerotatus taeniorhynchus Random amplified polymorphic dna-polymerase chain reaction Spatial autocorrelation population Genetics |
dc.subject.keyword.eng.fl_str_mv |
population Genetics |
description |
Ochlerotatus taeniorhynchus (Diptera: Culicidae) is a mosquito, which is an efficient vector of the virus causing epidemic-epizootic Venezuelan equine encephalitis in Colombia. This study used 9 random amplified polymorphic DNA (RAPD) markers to analyze the mosquito's genetic variability and genetic structure of 122 specimens in 7 populations from the Colombian Atlantic coast. Assuming that all loci were in Hardy-Weinberg equilibrium, diversity statistics and analyses were performed. The average number of amplified fragments for each primer was 8.3, and the size of these fragments ranged from 350 to 3600 bp. The expected average heterozygosity was 0.358 ± 0.103. The genetic heterogeneity among the populations studied was small (GST = 0.05 ± 0.01); meanwhile, the gene flow estimates (Nm = 7.32 ± 1.35) were high. In an identical way, the Nei's genetic distances obtained yielded very small values amongst the populations that were studied in this Colombian region. Furthermore, a spatial autocorrelation analysis with Moran's I index revealed a very weak, or inexistent, spatial genetic structure among these populations. The comparison of these results with those performed for other markers (isoenzymes and microsatellites) in populations of this same mosquito from the Colombian Atlantic coast was discussed. The results of our RAPD analysis showed scarce genetic differentiation among the mosquito populations on the Colombian Atlantic coast, which was probably determined by high gene flow levels. © FUNPEC-RP. |
publishDate |
2014 |
dc.date.created.spa.fl_str_mv |
2014 |
dc.date.accessioned.none.fl_str_mv |
2020-05-26T00:09:15Z |
dc.date.available.none.fl_str_mv |
2020-05-26T00:09:15Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.4238/2014.May.30.6 |
dc.identifier.issn.none.fl_str_mv |
16765680 |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/24147 |
url |
https://doi.org/10.4238/2014.May.30.6 https://repository.urosario.edu.co/handle/10336/24147 |
identifier_str_mv |
16765680 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.citationEndPage.none.fl_str_mv |
4123 |
dc.relation.citationIssue.none.fl_str_mv |
No. 2 |
dc.relation.citationStartPage.none.fl_str_mv |
4110 |
dc.relation.citationTitle.none.fl_str_mv |
Genetics and Molecular Research |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 13 |
dc.relation.ispartof.spa.fl_str_mv |
Genetics and Molecular Research, ISSN:16765680, Vol.13, No.2 (2014); pp. 4110-4123 |
dc.relation.uri.spa.fl_str_mv |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84901767197&doi=10.4238%2f2014.May.30.6&partnerID=40&md5=551772c3a134daf5815fd1ec96501a77 |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.acceso.spa.fl_str_mv |
Abierto (Texto Completo) |
rights_invalid_str_mv |
Abierto (Texto Completo) http://purl.org/coar/access_right/c_abf2 |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Fundacao de Pesquisas Cientificas de Ribeirao Preto |
institution |
Universidad del Rosario |
dc.source.instname.spa.fl_str_mv |
instname:Universidad del Rosario |
dc.source.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional EdocUR |
repository.name.fl_str_mv |
Repositorio institucional EdocUR |
repository.mail.fl_str_mv |
edocur@urosario.edu.co |
_version_ |
1814167549087055872 |