Analysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markers

Ochlerotatus taeniorhynchus (Diptera: Culicidae) is a mosquito, which is an efficient vector of the virus causing epidemic-epizootic Venezuelan equine encephalitis in Colombia. This study used 9 random amplified polymorphic DNA (RAPD) markers to analyze the mosquito's genetic variability and ge...

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Autores:
Tipo de recurso:
Fecha de publicación:
2014
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/24147
Acceso en línea:
https://doi.org/10.4238/2014.May.30.6
https://repository.urosario.edu.co/handle/10336/24147
Palabra clave:
Isoenzyme
Microsatellite dna
Article
Controlled study
Gene flow
Gene locus
Genetic distance
Genetic heterogeneity
Genetic variability
Genome analysis
Genome size
Genomic fragment
Heterozygosity
Insect genome
Microsatellite marker
Nonhuman
Nucleotide sequence
Ochlerotatus
Ochlerotatus taeniorhynchus
Population genetic structure
Random amplified polymorphic dna
Spatial autocorrelation analysis
Animal
Colombia
Genetic variability
Genetics
Heterozygote
Ochlerotatus
Population genetics
Animals
Colombia
Genetic variation
Heterozygote
Microsatellite repeats
Ochlerotatus
Random amplified polymorphic dna technique
Genetic heterogeneity
Genetic variability
Ochlerotatus taeniorhynchus
Random amplified polymorphic dna-polymerase chain reaction
Spatial autocorrelation
population
Genetics
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License
Abierto (Texto Completo)
id EDOCUR2_8d79edacb9a028f19a1500a7b3449781
oai_identifier_str oai:repository.urosario.edu.co:10336/24147
network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
spelling 25f0b849-c4d2-4ab9-ab9c-281620017867-13deb198c-1188-4d28-aa55-78c0f442fb54-1bd3e4519-7b71-477d-a72c-750bdf74c360-12020-05-26T00:09:15Z2020-05-26T00:09:15Z2014Ochlerotatus taeniorhynchus (Diptera: Culicidae) is a mosquito, which is an efficient vector of the virus causing epidemic-epizootic Venezuelan equine encephalitis in Colombia. This study used 9 random amplified polymorphic DNA (RAPD) markers to analyze the mosquito's genetic variability and genetic structure of 122 specimens in 7 populations from the Colombian Atlantic coast. Assuming that all loci were in Hardy-Weinberg equilibrium, diversity statistics and analyses were performed. The average number of amplified fragments for each primer was 8.3, and the size of these fragments ranged from 350 to 3600 bp. The expected average heterozygosity was 0.358 ± 0.103. The genetic heterogeneity among the populations studied was small (GST = 0.05 ± 0.01); meanwhile, the gene flow estimates (Nm = 7.32 ± 1.35) were high. In an identical way, the Nei's genetic distances obtained yielded very small values amongst the populations that were studied in this Colombian region. Furthermore, a spatial autocorrelation analysis with Moran's I index revealed a very weak, or inexistent, spatial genetic structure among these populations. The comparison of these results with those performed for other markers (isoenzymes and microsatellites) in populations of this same mosquito from the Colombian Atlantic coast was discussed. The results of our RAPD analysis showed scarce genetic differentiation among the mosquito populations on the Colombian Atlantic coast, which was probably determined by high gene flow levels. © FUNPEC-RP.application/pdfhttps://doi.org/10.4238/2014.May.30.616765680https://repository.urosario.edu.co/handle/10336/24147engFundacao de Pesquisas Cientificas de Ribeirao Preto4123No. 24110Genetics and Molecular ResearchVol. 13Genetics and Molecular Research, ISSN:16765680, Vol.13, No.2 (2014); pp. 4110-4123https://www.scopus.com/inward/record.uri?eid=2-s2.0-84901767197&doi=10.4238%2f2014.May.30.6&partnerID=40&md5=551772c3a134daf5815fd1ec96501a77Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURIsoenzymeMicrosatellite dnaArticleControlled studyGene flowGene locusGenetic distanceGenetic heterogeneityGenetic variabilityGenome analysisGenome sizeGenomic fragmentHeterozygosityInsect genomeMicrosatellite markerNonhumanNucleotide sequenceOchlerotatusOchlerotatus taeniorhynchusPopulation genetic structureRandom amplified polymorphic dnaSpatial autocorrelation analysisAnimalColombiaGenetic variabilityGeneticsHeterozygoteOchlerotatusPopulation geneticsAnimalsColombiaGenetic variationHeterozygoteMicrosatellite repeatsOchlerotatusRandom amplified polymorphic dna techniqueGenetic heterogeneityGenetic variabilityOchlerotatus taeniorhynchusRandom amplified polymorphic dna-polymerase chain reactionSpatial autocorrelationpopulationGeneticsAnalysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markersarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Bello, F JSegura, N ARuiz-Garcia, M10336/24147oai:repository.urosario.edu.co:10336/241472022-05-02 07:37:14.932427https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Analysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markers
title Analysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markers
spellingShingle Analysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markers
Isoenzyme
Microsatellite dna
Article
Controlled study
Gene flow
Gene locus
Genetic distance
Genetic heterogeneity
Genetic variability
Genome analysis
Genome size
Genomic fragment
Heterozygosity
Insect genome
Microsatellite marker
Nonhuman
Nucleotide sequence
Ochlerotatus
Ochlerotatus taeniorhynchus
Population genetic structure
Random amplified polymorphic dna
Spatial autocorrelation analysis
Animal
Colombia
Genetic variability
Genetics
Heterozygote
Ochlerotatus
Population genetics
Animals
Colombia
Genetic variation
Heterozygote
Microsatellite repeats
Ochlerotatus
Random amplified polymorphic dna technique
Genetic heterogeneity
Genetic variability
Ochlerotatus taeniorhynchus
Random amplified polymorphic dna-polymerase chain reaction
Spatial autocorrelation
population
Genetics
title_short Analysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markers
title_full Analysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markers
title_fullStr Analysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markers
title_full_unstemmed Analysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markers
title_sort Analysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markers
dc.subject.keyword.spa.fl_str_mv Isoenzyme
Microsatellite dna
Article
Controlled study
Gene flow
Gene locus
Genetic distance
Genetic heterogeneity
Genetic variability
Genome analysis
Genome size
Genomic fragment
Heterozygosity
Insect genome
Microsatellite marker
Nonhuman
Nucleotide sequence
Ochlerotatus
Ochlerotatus taeniorhynchus
Population genetic structure
Random amplified polymorphic dna
Spatial autocorrelation analysis
Animal
Colombia
Genetic variability
Genetics
Heterozygote
Ochlerotatus
Population genetics
Animals
Colombia
Genetic variation
Heterozygote
Microsatellite repeats
Ochlerotatus
Random amplified polymorphic dna technique
Genetic heterogeneity
Genetic variability
Ochlerotatus taeniorhynchus
Random amplified polymorphic dna-polymerase chain reaction
Spatial autocorrelation
topic Isoenzyme
Microsatellite dna
Article
Controlled study
Gene flow
Gene locus
Genetic distance
Genetic heterogeneity
Genetic variability
Genome analysis
Genome size
Genomic fragment
Heterozygosity
Insect genome
Microsatellite marker
Nonhuman
Nucleotide sequence
Ochlerotatus
Ochlerotatus taeniorhynchus
Population genetic structure
Random amplified polymorphic dna
Spatial autocorrelation analysis
Animal
Colombia
Genetic variability
Genetics
Heterozygote
Ochlerotatus
Population genetics
Animals
Colombia
Genetic variation
Heterozygote
Microsatellite repeats
Ochlerotatus
Random amplified polymorphic dna technique
Genetic heterogeneity
Genetic variability
Ochlerotatus taeniorhynchus
Random amplified polymorphic dna-polymerase chain reaction
Spatial autocorrelation
population
Genetics
dc.subject.keyword.eng.fl_str_mv population
Genetics
description Ochlerotatus taeniorhynchus (Diptera: Culicidae) is a mosquito, which is an efficient vector of the virus causing epidemic-epizootic Venezuelan equine encephalitis in Colombia. This study used 9 random amplified polymorphic DNA (RAPD) markers to analyze the mosquito's genetic variability and genetic structure of 122 specimens in 7 populations from the Colombian Atlantic coast. Assuming that all loci were in Hardy-Weinberg equilibrium, diversity statistics and analyses were performed. The average number of amplified fragments for each primer was 8.3, and the size of these fragments ranged from 350 to 3600 bp. The expected average heterozygosity was 0.358 ± 0.103. The genetic heterogeneity among the populations studied was small (GST = 0.05 ± 0.01); meanwhile, the gene flow estimates (Nm = 7.32 ± 1.35) were high. In an identical way, the Nei's genetic distances obtained yielded very small values amongst the populations that were studied in this Colombian region. Furthermore, a spatial autocorrelation analysis with Moran's I index revealed a very weak, or inexistent, spatial genetic structure among these populations. The comparison of these results with those performed for other markers (isoenzymes and microsatellites) in populations of this same mosquito from the Colombian Atlantic coast was discussed. The results of our RAPD analysis showed scarce genetic differentiation among the mosquito populations on the Colombian Atlantic coast, which was probably determined by high gene flow levels. © FUNPEC-RP.
publishDate 2014
dc.date.created.spa.fl_str_mv 2014
dc.date.accessioned.none.fl_str_mv 2020-05-26T00:09:15Z
dc.date.available.none.fl_str_mv 2020-05-26T00:09:15Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.4238/2014.May.30.6
dc.identifier.issn.none.fl_str_mv 16765680
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/24147
url https://doi.org/10.4238/2014.May.30.6
https://repository.urosario.edu.co/handle/10336/24147
identifier_str_mv 16765680
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 4123
dc.relation.citationIssue.none.fl_str_mv No. 2
dc.relation.citationStartPage.none.fl_str_mv 4110
dc.relation.citationTitle.none.fl_str_mv Genetics and Molecular Research
dc.relation.citationVolume.none.fl_str_mv Vol. 13
dc.relation.ispartof.spa.fl_str_mv Genetics and Molecular Research, ISSN:16765680, Vol.13, No.2 (2014); pp. 4110-4123
dc.relation.uri.spa.fl_str_mv https://www.scopus.com/inward/record.uri?eid=2-s2.0-84901767197&doi=10.4238%2f2014.May.30.6&partnerID=40&md5=551772c3a134daf5815fd1ec96501a77
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
rights_invalid_str_mv Abierto (Texto Completo)
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Fundacao de Pesquisas Cientificas de Ribeirao Preto
institution Universidad del Rosario
dc.source.instname.spa.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.spa.fl_str_mv reponame:Repositorio Institucional EdocUR
repository.name.fl_str_mv Repositorio institucional EdocUR
repository.mail.fl_str_mv edocur@urosario.edu.co
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