Effects of 17β-estradiol and tamoxifen on the induction of chromosomal abnormalities and on the expression of her2 gene in breast cancer cell lines

This study covers an area of great importance in the research of breast cancer, related to the study of the effects of both estrogens (E2) and anti-estrogens (Tamoxifen) on chromosomes and of modulation of gene expression. Considering that breast cancer is a very heterogeneous disease and that patie...

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Autores:
Tipo de recurso:
Fecha de publicación:
2014
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
spa
OAI Identifier:
oai:repository.urosario.edu.co:10336/10215
Acceso en línea:
https://doi.org/10.48713/10336_10215
http://repository.urosario.edu.co/handle/10336/10215
Palabra clave:
Breast Cancer
Chromosomal abnormalities
M-FISH
Tamoxifen
17B-Estradiol
Enfermedades
17β-estradiol
Ingeniería biomédica
Genética
Cromosomas
Breast Cancer
Chromosomal abnormalities
M-FISH
Tamoxifen
17B-estradiol
Rights
License
Abierto (Texto completo)
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dc.title.spa.fl_str_mv Effects of 17β-estradiol and tamoxifen on the induction of chromosomal abnormalities and on the expression of her2 gene in breast cancer cell lines
title Effects of 17β-estradiol and tamoxifen on the induction of chromosomal abnormalities and on the expression of her2 gene in breast cancer cell lines
spellingShingle Effects of 17β-estradiol and tamoxifen on the induction of chromosomal abnormalities and on the expression of her2 gene in breast cancer cell lines
Breast Cancer
Chromosomal abnormalities
M-FISH
Tamoxifen
17B-Estradiol
Enfermedades
17β-estradiol
Ingeniería biomédica
Genética
Cromosomas
Breast Cancer
Chromosomal abnormalities
M-FISH
Tamoxifen
17B-estradiol
title_short Effects of 17β-estradiol and tamoxifen on the induction of chromosomal abnormalities and on the expression of her2 gene in breast cancer cell lines
title_full Effects of 17β-estradiol and tamoxifen on the induction of chromosomal abnormalities and on the expression of her2 gene in breast cancer cell lines
title_fullStr Effects of 17β-estradiol and tamoxifen on the induction of chromosomal abnormalities and on the expression of her2 gene in breast cancer cell lines
title_full_unstemmed Effects of 17β-estradiol and tamoxifen on the induction of chromosomal abnormalities and on the expression of her2 gene in breast cancer cell lines
title_sort Effects of 17β-estradiol and tamoxifen on the induction of chromosomal abnormalities and on the expression of her2 gene in breast cancer cell lines
dc.contributor.advisor.none.fl_str_mv Sapino, Anna
dc.subject.spa.fl_str_mv Breast Cancer
Chromosomal abnormalities
M-FISH
Tamoxifen
17B-Estradiol
topic Breast Cancer
Chromosomal abnormalities
M-FISH
Tamoxifen
17B-Estradiol
Enfermedades
17β-estradiol
Ingeniería biomédica
Genética
Cromosomas
Breast Cancer
Chromosomal abnormalities
M-FISH
Tamoxifen
17B-estradiol
dc.subject.ddc.none.fl_str_mv Enfermedades
dc.subject.decs.spa.fl_str_mv 17β-estradiol
Ingeniería biomédica
Genética
Cromosomas
dc.subject.keyword.eng.fl_str_mv Breast Cancer
Chromosomal abnormalities
M-FISH
Tamoxifen
17B-estradiol
description This study covers an area of great importance in the research of breast cancer, related to the study of the effects of both estrogens (E2) and anti-estrogens (Tamoxifen) on chromosomes and of modulation of gene expression. Considering that breast cancer is a very heterogeneous disease and that patients respond differently to treatment, the identification of chromosomal abnormalities as well as genes responsive to 17β-estradiol (E2) and Tamoxifen (TAM) could provide the necessary framework to understand the complex effects of this hormone in target cells and could explain, at least in part, the development of cellular resistance to TAM treatment and the subsequent best therapeutic option. In this order of ideas, we determined the effects of E2 and TAM on the chromosomes and on the modulation of gene expression in four breast cancer cell lines, which represent three of the five subtypes of breast cancer known at present. The results are presented in six chapters - each one has a group of the results achieved around the cytogenetic characteristics and gene expression profiles of four cell lines and the effects of E2 and TAM incubation on those. The first chapter describes the main features of breast cancer, furthering the use and effects of E2 and TAM treatment.
publishDate 2014
dc.date.created.none.fl_str_mv 2014-07-29
dc.date.issued.none.fl_str_mv 2014
dc.date.accessioned.none.fl_str_mv 2015-02-23T12:43:20Z
dc.date.available.none.fl_str_mv 2015-02-23T12:43:20Z
dc.type.eng.fl_str_mv doctoralThesis
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_db06
dc.type.spa.spa.fl_str_mv Tesis de doctorado
dc.identifier.doi.none.fl_str_mv https://doi.org/10.48713/10336_10215
dc.identifier.uri.none.fl_str_mv http://repository.urosario.edu.co/handle/10336/10215
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http://repository.urosario.edu.co/handle/10336/10215
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dc.publisher.spa.fl_str_mv Universidad del Rosario
dc.publisher.department.spa.fl_str_mv Facultad de Ciencias Naturales y Matemáticas
dc.publisher.program.spa.fl_str_mv Doctorado en Ciencias Biomédicas
institution Universidad del Rosario
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spelling Sapino, Anna586ecb0f-4765-4c64-a7c9-b1886c406993-1Rondón Lagos, Sandra MilenaDoctor en Ciencias Biomédicas7f186837-8863-4241-88bf-47e00a6be601-12015-02-23T12:43:20Z2015-02-23T12:43:20Z2014-07-292014This study covers an area of great importance in the research of breast cancer, related to the study of the effects of both estrogens (E2) and anti-estrogens (Tamoxifen) on chromosomes and of modulation of gene expression. Considering that breast cancer is a very heterogeneous disease and that patients respond differently to treatment, the identification of chromosomal abnormalities as well as genes responsive to 17β-estradiol (E2) and Tamoxifen (TAM) could provide the necessary framework to understand the complex effects of this hormone in target cells and could explain, at least in part, the development of cellular resistance to TAM treatment and the subsequent best therapeutic option. In this order of ideas, we determined the effects of E2 and TAM on the chromosomes and on the modulation of gene expression in four breast cancer cell lines, which represent three of the five subtypes of breast cancer known at present. The results are presented in six chapters - each one has a group of the results achieved around the cytogenetic characteristics and gene expression profiles of four cell lines and the effects of E2 and TAM incubation on those. The first chapter describes the main features of breast cancer, furthering the use and effects of E2 and TAM treatment. Eracol y Colcienciasapplication/pdfhttps://doi.org/10.48713/10336_10215 http://repository.urosario.edu.co/handle/10336/10215spaUniversidad del RosarioFacultad de Ciencias Naturales y MatemáticasDoctorado en Ciencias BiomédicasAbierto (Texto completo)EL AUTOR, manifiesta que la obra objeto de la presente autorización es original y la realizó sin violar o usurpar derechos de autor de terceros, por lo tanto la obra es de exclusiva autoría y tiene la titularidad sobre la misma. PARGRAFO: En caso de presentarse cualquier reclamación o acción por parte de un tercero en cuanto a los derechos de autor sobre la obra en cuestión, EL AUTOR, asumirá toda la responsabilidad, y saldrá en defensa de los derechos aquí autorizados; para todos los efectos la universidad actúa como un tercero de buena fe. EL AUTOR, autoriza a LA UNIVERSIDAD DEL ROSARIO, para que en los términos establecidos en la Ley 23 de 1982, Ley 44 de 1993, Decisión andina 351 de 1993, Decreto 460 de 1995 y demás normas generales sobre la materia, utilice y use la obra objeto de la presente autorización. -------------------------------------- POLITICA DE TRATAMIENTO DE DATOS PERSONALES. Declaro que autorizo previa y de forma informada el tratamiento de mis datos personales por parte de LA UNIVERSIDAD DEL ROSARIO para fines académicos y en aplicación de convenios con terceros o servicios conexos con actividades propias de la academia, con estricto cumplimiento de los principios de ley. Para el correcto ejercicio de mi derecho de habeas data cuento con la cuenta de correo habeasdata@urosario.edu.co, donde previa identificación podré solicitar la consulta, corrección y supresión de mis datos.http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURBreast CancerChromosomal abnormalitiesM-FISHTamoxifen17B-EstradiolEnfermedades61660017β-estradiolIngeniería biomédicaGenéticaCromosomasBreast CancerChromosomal abnormalitiesM-FISHTamoxifen17B-estradiolEffects of 17β-estradiol and tamoxifen on the induction of chromosomal abnormalities and on the expression of her2 gene in breast cancer cell linesdoctoralThesisTesis de doctoradohttp://purl.org/coar/resource_type/c_db06Escuela de Medicina y Ciencias de la SaludORIGINALChapter 1 Final.pdfChapter 1 Final.pdfapplication/pdf2560994https://repository.urosario.edu.co/bitstreams/e5bd42bf-9740-4d28-8f00-498ae5baa5ac/download085432fb41925e1175d2cb03a785bab4MD51Chapter 2 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