Analytical validation of quantitative real-time PCR methods for quantification of trypanosoma cruzi DNA in blood samples from chagas disease patients

An international study was performed by 26 experienced PCR laboratories from 14 countries to assess the performance of duplex quantitative real-time PCR (qPCR) strategies on the basis of TaqMan probes for detection and quantification of parasitic loads in peripheral blood samples from Chagas disease...

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Tipo de recurso:
Fecha de publicación:
2015
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/26203
Acceso en línea:
https://doi.org/10.1016/j.jmoldx.2015.04.010
https://repository.urosario.edu.co/handle/10336/26203
Palabra clave:
Analytical validation
DNA
Trypanosoma rangeli
Leishmania spp
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dc.title.spa.fl_str_mv Analytical validation of quantitative real-time PCR methods for quantification of trypanosoma cruzi DNA in blood samples from chagas disease patients
dc.title.TranslatedTitle.spa.fl_str_mv Validación analítica de métodos cuantitativos de PCR en tiempo real para la cuantificación del ADN de Trypanosoma cruzi en muestras de sangre de pacientes con enfermedad de Chagas
title Analytical validation of quantitative real-time PCR methods for quantification of trypanosoma cruzi DNA in blood samples from chagas disease patients
spellingShingle Analytical validation of quantitative real-time PCR methods for quantification of trypanosoma cruzi DNA in blood samples from chagas disease patients
Analytical validation
DNA
Trypanosoma rangeli
Leishmania spp
title_short Analytical validation of quantitative real-time PCR methods for quantification of trypanosoma cruzi DNA in blood samples from chagas disease patients
title_full Analytical validation of quantitative real-time PCR methods for quantification of trypanosoma cruzi DNA in blood samples from chagas disease patients
title_fullStr Analytical validation of quantitative real-time PCR methods for quantification of trypanosoma cruzi DNA in blood samples from chagas disease patients
title_full_unstemmed Analytical validation of quantitative real-time PCR methods for quantification of trypanosoma cruzi DNA in blood samples from chagas disease patients
title_sort Analytical validation of quantitative real-time PCR methods for quantification of trypanosoma cruzi DNA in blood samples from chagas disease patients
dc.subject.keyword.spa.fl_str_mv Analytical validation
DNA
Trypanosoma rangeli
Leishmania spp
topic Analytical validation
DNA
Trypanosoma rangeli
Leishmania spp
description An international study was performed by 26 experienced PCR laboratories from 14 countries to assess the performance of duplex quantitative real-time PCR (qPCR) strategies on the basis of TaqMan probes for detection and quantification of parasitic loads in peripheral blood samples from Chagas disease patients. Two methods were studied: Satellite DNA (SatDNA) qPCR and kinetoplastid DNA (kDNA) qPCR. Both methods included an internal amplification control. R
publishDate 2015
dc.date.created.spa.fl_str_mv 2015-09-01
dc.date.accessioned.none.fl_str_mv 2020-08-06T16:20:57Z
dc.date.available.none.fl_str_mv 2020-08-06T16:20:57Z
dc.type.eng.fl_str_mv article
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dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1016/j.jmoldx.2015.04.010
dc.identifier.issn.none.fl_str_mv ISSN: 1525-1578
EISSN: 1943-7811
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/26203
url https://doi.org/10.1016/j.jmoldx.2015.04.010
https://repository.urosario.edu.co/handle/10336/26203
identifier_str_mv ISSN: 1525-1578
EISSN: 1943-7811
dc.language.iso.spa.fl_str_mv eng
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dc.relation.citationIssue.none.fl_str_mv No. 5
dc.relation.citationStartPage.none.fl_str_mv 605
dc.relation.citationTitle.none.fl_str_mv The Journal of Molecular Diagnostics
dc.relation.citationVolume.none.fl_str_mv Vol. 17
dc.relation.ispartof.spa.fl_str_mv The Journal of Molecular Diagnostics, ISSN: 1525-1578;EISSN: 1943-7811 Vol.17, No.5 (2015); pp.605-615
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dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv American Society for Investigative Pathology and the Association for Molecular Pathology
Elsevier
dc.source.spa.fl_str_mv The Journal of Molecular Diagnostics
institution Universidad del Rosario
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