Slight temperature changes cause rapid transcriptomic responses in Trypanosoma cruzi metacyclic trypomastigotes

Background: Severe changes in temperature can affect the behavior and ecology of some infectious agents. Trypanosoma cruzi is a protozoan that causes Chagas disease. This parasite has high genetic variability and can be divided into six discrete typing units (DTUs). Trypanosoma cruzi also has a comp...

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Tipo de recurso:
Fecha de publicación:
2020
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/25026
Acceso en línea:
https://repository.urosario.edu.co/handle/10336/25026
Palabra clave:
DTUs
Metacyclic trypomastigotes
RNAseq
Temperature
Transcriptomic
Trypanosoma cruzi
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License
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id EDOCUR2_836852df95675aee4ed3c4066dd62078
oai_identifier_str oai:repository.urosario.edu.co:10336/25026
network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
spelling b1ad6f0f-e1f9-44ed-b605-775349559b03453006a5-2ec3-4faf-8e32-a9d7075d519ddc30c120-c40b-4f9c-92f2-245f57c6dcde488d1a7b-b879-40bf-9521-71b098c186be65aad41b-1b4a-46d3-9d0e-a4c8f687e8f510117161186002020-06-11T13:22:07Z2020-06-11T13:22:07Z2020Background: Severe changes in temperature can affect the behavior and ecology of some infectious agents. Trypanosoma cruzi is a protozoan that causes Chagas disease. This parasite has high genetic variability and can be divided into six discrete typing units (DTUs). Trypanosoma cruzi also has a complex life-cycle, which includes the process of metacyclogenesis when non-infective epimastigote forms are differentiated into infective metacyclic trypomastigotes (MT). Studies in triatomines have shown that changes in temperature also affect the number and viability of MT. Methods: The objective of this study was to evaluate how temperature affects the transcriptional profiles of T. cruzi I and II (TcI and TcII) MT by exposing parasites to two temperatures (27 °C and 28 °C) and comparing those to normal culture conditions at 26 °C. Subsequently, RNA-seq was conducted and differentially expressed genes were quantified and associated to metabolic pathways. Results: A statistically significant difference was observed in the number of MT between the temperatures evaluated and the control, TcII DTU was not strongly affected to exposure to high temperatures compared to TcI. Similar results were found when we analyzed gene expression in this DTU, with the greatest number of differentially expressed genes being observed at 28 °C, which could indicate a dysregulation of different signaling pathways under this temperature. Chromosome analysis indicated that chromosome 1 harbored the highest number of changes for both DTUs for all thermal treatments. Finally, gene ontology (GO) analyses showed a decrease in the coding RNAs involved in the regulation of processes related to the metabolism of lipids and carbohydrates, the evasion of oxidative stress, and proteolysis and phosphorylation processes, and a decrease in RNAs coding to ribosomal proteins in TcI and TcII, along with an increase in the expression of surface metalloprotease GP63 in TcII. Conclusions: Slight temperature shifts lead to increased cell death of metacyclic trypomastigotes because of the deregulation of gene expression of different processes essential for the TcI and TcII DTUs of T. cruzi.[Figure not available: see fulltext.] © 2020 The Author(s).application/pdf17563305https://repository.urosario.edu.co/handle/10336/25026engBioMed Central Ltd.No. 1Parasites and VectorsVol. 13Parasites and Vectors, ISBN: 17563305, Vol.13, No.1 (2020); pp. -https://www.scopus.com/inward/record.uri?eid=2-s2.0-85084785414&doi=10.1186%2fs13071-020-04125-y&partnerID=40&md5=4949ebf83c056fd5db745b5cfcc05df7https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-020-04125-yAbierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURDTUsMetacyclic trypomastigotesRNAseqTemperatureTranscriptomicTrypanosoma cruziSlight temperature changes cause rapid transcriptomic responses in Trypanosoma cruzi metacyclic trypomastigotesarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Cruz-Saavedra, L.Muñoz, MarinaPatiño, L. H.Vallejo, G. A.Guhl, F.Ramírez, Juan David10336/25026oai:repository.urosario.edu.co:10336/250262023-06-01 11:21:23.699https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Slight temperature changes cause rapid transcriptomic responses in Trypanosoma cruzi metacyclic trypomastigotes
title Slight temperature changes cause rapid transcriptomic responses in Trypanosoma cruzi metacyclic trypomastigotes
spellingShingle Slight temperature changes cause rapid transcriptomic responses in Trypanosoma cruzi metacyclic trypomastigotes
DTUs
Metacyclic trypomastigotes
RNAseq
Temperature
Transcriptomic
Trypanosoma cruzi
title_short Slight temperature changes cause rapid transcriptomic responses in Trypanosoma cruzi metacyclic trypomastigotes
title_full Slight temperature changes cause rapid transcriptomic responses in Trypanosoma cruzi metacyclic trypomastigotes
title_fullStr Slight temperature changes cause rapid transcriptomic responses in Trypanosoma cruzi metacyclic trypomastigotes
title_full_unstemmed Slight temperature changes cause rapid transcriptomic responses in Trypanosoma cruzi metacyclic trypomastigotes
title_sort Slight temperature changes cause rapid transcriptomic responses in Trypanosoma cruzi metacyclic trypomastigotes
dc.subject.keyword.spa.fl_str_mv DTUs
Metacyclic trypomastigotes
RNAseq
Temperature
Transcriptomic
Trypanosoma cruzi
topic DTUs
Metacyclic trypomastigotes
RNAseq
Temperature
Transcriptomic
Trypanosoma cruzi
description Background: Severe changes in temperature can affect the behavior and ecology of some infectious agents. Trypanosoma cruzi is a protozoan that causes Chagas disease. This parasite has high genetic variability and can be divided into six discrete typing units (DTUs). Trypanosoma cruzi also has a complex life-cycle, which includes the process of metacyclogenesis when non-infective epimastigote forms are differentiated into infective metacyclic trypomastigotes (MT). Studies in triatomines have shown that changes in temperature also affect the number and viability of MT. Methods: The objective of this study was to evaluate how temperature affects the transcriptional profiles of T. cruzi I and II (TcI and TcII) MT by exposing parasites to two temperatures (27 °C and 28 °C) and comparing those to normal culture conditions at 26 °C. Subsequently, RNA-seq was conducted and differentially expressed genes were quantified and associated to metabolic pathways. Results: A statistically significant difference was observed in the number of MT between the temperatures evaluated and the control, TcII DTU was not strongly affected to exposure to high temperatures compared to TcI. Similar results were found when we analyzed gene expression in this DTU, with the greatest number of differentially expressed genes being observed at 28 °C, which could indicate a dysregulation of different signaling pathways under this temperature. Chromosome analysis indicated that chromosome 1 harbored the highest number of changes for both DTUs for all thermal treatments. Finally, gene ontology (GO) analyses showed a decrease in the coding RNAs involved in the regulation of processes related to the metabolism of lipids and carbohydrates, the evasion of oxidative stress, and proteolysis and phosphorylation processes, and a decrease in RNAs coding to ribosomal proteins in TcI and TcII, along with an increase in the expression of surface metalloprotease GP63 in TcII. Conclusions: Slight temperature shifts lead to increased cell death of metacyclic trypomastigotes because of the deregulation of gene expression of different processes essential for the TcI and TcII DTUs of T. cruzi.[Figure not available: see fulltext.] © 2020 The Author(s).
publishDate 2020
dc.date.accessioned.none.fl_str_mv 2020-06-11T13:22:07Z
dc.date.available.none.fl_str_mv 2020-06-11T13:22:07Z
dc.date.created.spa.fl_str_mv 2020
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.issn.none.fl_str_mv 17563305
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/25026
identifier_str_mv 17563305
url https://repository.urosario.edu.co/handle/10336/25026
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.citationIssue.none.fl_str_mv No. 1
dc.relation.citationTitle.none.fl_str_mv Parasites and Vectors
dc.relation.citationVolume.none.fl_str_mv Vol. 13
dc.relation.ispartof.spa.fl_str_mv Parasites and Vectors, ISBN: 17563305, Vol.13, No.1 (2020); pp. -
dc.relation.uri.spa.fl_str_mv https://www.scopus.com/inward/record.uri?eid=2-s2.0-85084785414&doi=10.1186%2fs13071-020-04125-y&partnerID=40&md5=4949ebf83c056fd5db745b5cfcc05df7
dc.relation.uri.none.fl_str_mv https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-020-04125-y
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
rights_invalid_str_mv Abierto (Texto Completo)
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv BioMed Central Ltd.
institution Universidad del Rosario
dc.source.instname.spa.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.spa.fl_str_mv reponame:Repositorio Institucional EdocUR
repository.name.fl_str_mv Repositorio institucional EdocUR
repository.mail.fl_str_mv edocur@urosario.edu.co
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