Abnormality of Oct-1 DNA binding in T cells from Sjogren's syndrome patients

Primary Sjögren's syndrome (SS) is an autoimmune rheumatic disease characterized by T cell hypoactivity. To understand the diminished T cell response to activation signals, we measured nucleoprotein DNA?binding activities regulating gene expression during T cell activation using the electrophor...

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Autores:
Tipo de recurso:
Fecha de publicación:
1996
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/27897
Acceso en línea:
https://doi.org/10.1002/eji.1830260906
https://repository.urosario.edu.co/handle/10336/27897
Palabra clave:
Primary Sjögren's syndrome
T cell hypoactivity
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License
Restringido (Acceso a grupos específicos)
id EDOCUR2_80eea126cdd03d00558376c57c474ff7
oai_identifier_str oai:repository.urosario.edu.co:10336/27897
network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
spelling 8c4c6a98-94a0-4396-96ef-0c3e9eade8a2-1fffce164-5bd1-4869-aea0-5cdad1c2d439-1861f6737-05e5-4f8f-a018-3ed9ea26ad6c-176dd2b4d-1a43-4960-b227-73d5787978a3-1878de387-4ba0-4098-b607-65e3b9d7bb87-1194747786002020-08-19T14:44:30Z2020-08-19T14:44:30Z1996Primary Sjögren's syndrome (SS) is an autoimmune rheumatic disease characterized by T cell hypoactivity. To understand the diminished T cell response to activation signals, we measured nucleoprotein DNA?binding activities regulating gene expression during T cell activation using the electrophoretic mobility shift assay. Peripheral blood lymphocytes from 9/19 SS patients were found to be defective in their ability to bind an octomer sequence (Oct?1). This Oct?1?binding phenotype remained stable in culture for up to 3 days prior to activation. This abnormality was not seen in resting T cells nor T cells from patients with systemic lupus erythematosus, rheumatoid arthritis (RA), or SS accompanied by RA. The SS Oct?1 DNA?binding abnormality correlated significantly with an inability of cells to exit the G0/G1 cell cycle phase when stimulated in vitro . Importantly, nucleoprotein extracts showing decreased DNA?binding activity had normal amounts of Oct?1 proteins as determined by immunoprecipitation, implying a functional defect in the Oct?1 protein. Moreover, defective DNA binding was corrected by treatment with acid phosphatase.application/pdfhttps://doi.org/10.1002/eji.1830260906ISSN: 0014-2980EISSN: 1521-4141https://repository.urosario.edu.co/handle/10336/27897engJohn Wiley & Sons2011No. 92006European Journal of ImmunologyVol. 26European Journal of Immunology, ISSN: 0014-2980;EISSN:1521-4141, Vol.26, No.9 (September, 1996); pp. 2006-2011https://onlinelibrary.wiley.com/doi/abs/10.1002/eji.1830260906Restringido (Acceso a grupos específicos)http://purl.org/coar/access_right/c_16ecEuropean Journal of Immunologyinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURPrimary Sjögren's syndromeT cell hypoactivityAbnormality of Oct-1 DNA binding in T cells from Sjogren's syndrome patientsAnormalidad de la unión al ADN de Oct-1 en las células T de pacientes con síndrome de SjogrenarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Flescher, EliezerVela Roch, NormaOgawa, NoriyoshiNakabayashi, ToruEscalante, AgustinAnaya, Juan-Manuel10336/27897oai:repository.urosario.edu.co:10336/278972021-06-03 00:51:07.315https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Abnormality of Oct-1 DNA binding in T cells from Sjogren's syndrome patients
dc.title.TranslatedTitle.spa.fl_str_mv Anormalidad de la unión al ADN de Oct-1 en las células T de pacientes con síndrome de Sjogren
title Abnormality of Oct-1 DNA binding in T cells from Sjogren's syndrome patients
spellingShingle Abnormality of Oct-1 DNA binding in T cells from Sjogren's syndrome patients
Primary Sjögren's syndrome
T cell hypoactivity
title_short Abnormality of Oct-1 DNA binding in T cells from Sjogren's syndrome patients
title_full Abnormality of Oct-1 DNA binding in T cells from Sjogren's syndrome patients
title_fullStr Abnormality of Oct-1 DNA binding in T cells from Sjogren's syndrome patients
title_full_unstemmed Abnormality of Oct-1 DNA binding in T cells from Sjogren's syndrome patients
title_sort Abnormality of Oct-1 DNA binding in T cells from Sjogren's syndrome patients
dc.subject.keyword.spa.fl_str_mv Primary Sjögren's syndrome
T cell hypoactivity
topic Primary Sjögren's syndrome
T cell hypoactivity
description Primary Sjögren's syndrome (SS) is an autoimmune rheumatic disease characterized by T cell hypoactivity. To understand the diminished T cell response to activation signals, we measured nucleoprotein DNA?binding activities regulating gene expression during T cell activation using the electrophoretic mobility shift assay. Peripheral blood lymphocytes from 9/19 SS patients were found to be defective in their ability to bind an octomer sequence (Oct?1). This Oct?1?binding phenotype remained stable in culture for up to 3 days prior to activation. This abnormality was not seen in resting T cells nor T cells from patients with systemic lupus erythematosus, rheumatoid arthritis (RA), or SS accompanied by RA. The SS Oct?1 DNA?binding abnormality correlated significantly with an inability of cells to exit the G0/G1 cell cycle phase when stimulated in vitro . Importantly, nucleoprotein extracts showing decreased DNA?binding activity had normal amounts of Oct?1 proteins as determined by immunoprecipitation, implying a functional defect in the Oct?1 protein. Moreover, defective DNA binding was corrected by treatment with acid phosphatase.
publishDate 1996
dc.date.created.spa.fl_str_mv 1996
dc.date.accessioned.none.fl_str_mv 2020-08-19T14:44:30Z
dc.date.available.none.fl_str_mv 2020-08-19T14:44:30Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1002/eji.1830260906
dc.identifier.issn.none.fl_str_mv ISSN: 0014-2980
EISSN: 1521-4141
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/27897
url https://doi.org/10.1002/eji.1830260906
https://repository.urosario.edu.co/handle/10336/27897
identifier_str_mv ISSN: 0014-2980
EISSN: 1521-4141
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 2011
dc.relation.citationIssue.none.fl_str_mv No. 9
dc.relation.citationStartPage.none.fl_str_mv 2006
dc.relation.citationTitle.none.fl_str_mv European Journal of Immunology
dc.relation.citationVolume.none.fl_str_mv Vol. 26
dc.relation.ispartof.spa.fl_str_mv European Journal of Immunology, ISSN: 0014-2980;EISSN:1521-4141, Vol.26, No.9 (September, 1996); pp. 2006-2011
dc.relation.uri.spa.fl_str_mv https://onlinelibrary.wiley.com/doi/abs/10.1002/eji.1830260906
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_16ec
dc.rights.acceso.spa.fl_str_mv Restringido (Acceso a grupos específicos)
rights_invalid_str_mv Restringido (Acceso a grupos específicos)
http://purl.org/coar/access_right/c_16ec
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv John Wiley & Sons
dc.source.spa.fl_str_mv European Journal of Immunology
institution Universidad del Rosario
dc.source.instname.none.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.none.fl_str_mv reponame:Repositorio Institucional EdocUR
repository.name.fl_str_mv Repositorio institucional EdocUR
repository.mail.fl_str_mv edocur@urosario.edu.co
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