Simultaneous detection of Plasmodium vivax dhfr, dhps, mdr1 and crt-o resistance-associated mutations in the Colombian Amazonian region

Background: Malaria continues being a public health problem worldwide. Plasmodium vivax is the species causing the largest number of cases of malaria in Asia and South America. Due to the lack of a completely effective anti-malarial vaccine, controlling this disease has been based on transmission vector management, rapid diagnosis and suitable treatment. However, parasite resistance to anti-malarial drugs has become a major yet-to-be-overcome challenge. This study was thus aimed at determining pvmdr1, pvdhfr, pvdhps and pvcrt-o gene mutations and haplotypes from field samples obtained from an endemic area in the Colombian Amazonian region. Methods: Fifty samples of parasite DNA infected by a single P. vivax strain from symptomatic patients from the Amazonas department in Colombia were analysed by PCR and the pvdhfr, pvdhps, pvmdr1 and pvcrt-o genes were sequenced. Diversity estimators were calculated from the sequences and the haplotypes circulating in the Colombian Amazonian region were obtained. Conclusion: pvdhfr, pvdhps, pvmdr1 and pvcrt-o genes in the Colombian Amazonian region are characterized by low genetic diversity. Some resistance-associated mutations were found circulating in this population. New variants are also being reported. A selective sweep signal was located in pvdhfr and pvmdr1 genes, suggesting that these mutations (or some of them) could be providing an adaptive advantage. © 2018 The Author(s).

• Fecha de publicación: 2018
• Institución: Universidad del Rosario
• Repositorio: Repositorio EdocUR - U. Rosario
• Idioma: eng
• OAI Identifier: oai:repository.urosario.edu.co:10336/20382
• Acceso en línea: https://repository.urosario.edu.co/handle/10336/20382
• Palabra clave: Malaria Vaccine; Colombia; Crt O Gene; Dhfr Gene; Dhps Gene; Disease Surveillance; Electrophoresis; Endemic Disease; Gene; Gene Location; Gene Mutation; Gene Sequence; Genetic Association; Genetic Polymorphism; Genetic Resistance; Genetic Selection; Genetic Variability; Haplotype; Human; Malaria Control; Mdr1 Gene; Nonhuman; Nucleotide Sequence; Plasmodium Vivax; Plasmodium Vivax Malaria; Polymerase Chain Reaction; Vacuna contra la malaria; Crt O génica; Colombia; Enfermedades; Article; Malaria; Plasmodium
• License: Abierto (Texto Completo)