Characterising a microsatellite for DRB typing in Aotus vociferans and Aotus nancymaae (Platyrrhini)

Non-human primates belonging to the Aotus genus have been shown to be excellent experimental models for evaluating drugs and vaccine candidates against malaria and other human diseases. The immune system of this animal model must be characterised to assess whether the results obtained here can be ex...

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Autores:
Tipo de recurso:
Fecha de publicación:
2014
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/18702
Acceso en línea:
https://doi.org/10.1371/journal.pone.0096973
http://repository.urosario.edu.co/handle/10336/18702
Palabra clave:
ADN complementario
Antígeno Hla Dr
Antígeno Hla Drb1
Antígeno Hla Drb3
Antígeno mayor de histocompatibilidad clase 1
Antígeno mayor de histocompatibilidad clase 2
Hla antígeno clase 2
ADN microsatélite
Alelos
Célula animal
Aotus Vociferans
Estudio controlado
Secuencia de ADN
Exón
Análisis genético
Genotipo
Técnica de genotipado
Intrón
Mhc Drb Gen
Mhc Drb1 Gen
Mhc Drb3 Gen
Marcador de microsatélite
Evolución Molecular
Filogenia molecular
Árbol filogenético
Ornitorrinco
Alineación de secuencia
Repetición corta en tándem
Aótidos
Repeticiones de microsatélites
Microsatellite Repeats
Histocompatibility Antigens Class Ii
Aotidae
Short Tandem Repeat
Sequence Alignment
Platyrrhini
Phylogenetic Tree
Molecular Phylogeny
Molecular Evolution
Microsatellite Marker
Mhc Drb3 Gene
Mhc Drb1 Gene
Mhc Drb Gene
Intron
Genotyping Technique
Genotype
Genetic Analysis
Exon
Dna Sequence
Controlled Study
Aotus Nancymaae
Animal Cell
Microsatellite Dna
Allele
Hla Antigen Class 2
Major Histocompatibility Antigen Class 2
Hla Drb3 Antigen
Major Histocompatibility Antigen Class 1
Hla Drb1 Antigen
Hla Dr Antigen
Complementary Dna
Aotus Vociferans
Malaria
Histocompatibilidad
Experimentación con animales
Primates
Rights
License
Abierto (Texto Completo)
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repository_id_str
dc.title.spa.fl_str_mv Characterising a microsatellite for DRB typing in Aotus vociferans and Aotus nancymaae (Platyrrhini)
title Characterising a microsatellite for DRB typing in Aotus vociferans and Aotus nancymaae (Platyrrhini)
spellingShingle Characterising a microsatellite for DRB typing in Aotus vociferans and Aotus nancymaae (Platyrrhini)
ADN complementario
Antígeno Hla Dr
Antígeno Hla Drb1
Antígeno Hla Drb3
Antígeno mayor de histocompatibilidad clase 1
Antígeno mayor de histocompatibilidad clase 2
Hla antígeno clase 2
ADN microsatélite
Alelos
Célula animal
Aotus Vociferans
Estudio controlado
Secuencia de ADN
Exón
Análisis genético
Genotipo
Técnica de genotipado
Intrón
Mhc Drb Gen
Mhc Drb1 Gen
Mhc Drb3 Gen
Marcador de microsatélite
Evolución Molecular
Filogenia molecular
Árbol filogenético
Ornitorrinco
Alineación de secuencia
Repetición corta en tándem
Aótidos
Repeticiones de microsatélites
Microsatellite Repeats
Histocompatibility Antigens Class Ii
Aotidae
Short Tandem Repeat
Sequence Alignment
Platyrrhini
Phylogenetic Tree
Molecular Phylogeny
Molecular Evolution
Microsatellite Marker
Mhc Drb3 Gene
Mhc Drb1 Gene
Mhc Drb Gene
Intron
Genotyping Technique
Genotype
Genetic Analysis
Exon
Dna Sequence
Controlled Study
Aotus Nancymaae
Animal Cell
Microsatellite Dna
Allele
Hla Antigen Class 2
Major Histocompatibility Antigen Class 2
Hla Drb3 Antigen
Major Histocompatibility Antigen Class 1
Hla Drb1 Antigen
Hla Dr Antigen
Complementary Dna
Aotus Vociferans
Malaria
Histocompatibilidad
Experimentación con animales
Primates
title_short Characterising a microsatellite for DRB typing in Aotus vociferans and Aotus nancymaae (Platyrrhini)
title_full Characterising a microsatellite for DRB typing in Aotus vociferans and Aotus nancymaae (Platyrrhini)
title_fullStr Characterising a microsatellite for DRB typing in Aotus vociferans and Aotus nancymaae (Platyrrhini)
title_full_unstemmed Characterising a microsatellite for DRB typing in Aotus vociferans and Aotus nancymaae (Platyrrhini)
title_sort Characterising a microsatellite for DRB typing in Aotus vociferans and Aotus nancymaae (Platyrrhini)
dc.subject.spa.fl_str_mv ADN complementario
Antígeno Hla Dr
Antígeno Hla Drb1
Antígeno Hla Drb3
Antígeno mayor de histocompatibilidad clase 1
Antígeno mayor de histocompatibilidad clase 2
Hla antígeno clase 2
ADN microsatélite
Alelos
Célula animal
Aotus Vociferans
Estudio controlado
Secuencia de ADN
Exón
Análisis genético
Genotipo
Técnica de genotipado
Intrón
Mhc Drb Gen
Mhc Drb1 Gen
Mhc Drb3 Gen
Marcador de microsatélite
Evolución Molecular
Filogenia molecular
Árbol filogenético
Ornitorrinco
Alineación de secuencia
Repetición corta en tándem
Aótidos
Repeticiones de microsatélites
topic ADN complementario
Antígeno Hla Dr
Antígeno Hla Drb1
Antígeno Hla Drb3
Antígeno mayor de histocompatibilidad clase 1
Antígeno mayor de histocompatibilidad clase 2
Hla antígeno clase 2
ADN microsatélite
Alelos
Célula animal
Aotus Vociferans
Estudio controlado
Secuencia de ADN
Exón
Análisis genético
Genotipo
Técnica de genotipado
Intrón
Mhc Drb Gen
Mhc Drb1 Gen
Mhc Drb3 Gen
Marcador de microsatélite
Evolución Molecular
Filogenia molecular
Árbol filogenético
Ornitorrinco
Alineación de secuencia
Repetición corta en tándem
Aótidos
Repeticiones de microsatélites
Microsatellite Repeats
Histocompatibility Antigens Class Ii
Aotidae
Short Tandem Repeat
Sequence Alignment
Platyrrhini
Phylogenetic Tree
Molecular Phylogeny
Molecular Evolution
Microsatellite Marker
Mhc Drb3 Gene
Mhc Drb1 Gene
Mhc Drb Gene
Intron
Genotyping Technique
Genotype
Genetic Analysis
Exon
Dna Sequence
Controlled Study
Aotus Nancymaae
Animal Cell
Microsatellite Dna
Allele
Hla Antigen Class 2
Major Histocompatibility Antigen Class 2
Hla Drb3 Antigen
Major Histocompatibility Antigen Class 1
Hla Drb1 Antigen
Hla Dr Antigen
Complementary Dna
Aotus Vociferans
Malaria
Histocompatibilidad
Experimentación con animales
Primates
dc.subject.keyword.eng.fl_str_mv Microsatellite Repeats
Histocompatibility Antigens Class Ii
Aotidae
Short Tandem Repeat
Sequence Alignment
Platyrrhini
Phylogenetic Tree
Molecular Phylogeny
Molecular Evolution
Microsatellite Marker
Mhc Drb3 Gene
Mhc Drb1 Gene
Mhc Drb Gene
Intron
Genotyping Technique
Genotype
Genetic Analysis
Exon
Dna Sequence
Controlled Study
Aotus Nancymaae
Animal Cell
Microsatellite Dna
Allele
Hla Antigen Class 2
Major Histocompatibility Antigen Class 2
Hla Drb3 Antigen
Major Histocompatibility Antigen Class 1
Hla Drb1 Antigen
Hla Dr Antigen
Complementary Dna
dc.subject.keyword.lat.fl_str_mv Aotus Vociferans
dc.subject.lemb.spa.fl_str_mv Malaria
Histocompatibilidad
Experimentación con animales
Primates
description Non-human primates belonging to the Aotus genus have been shown to be excellent experimental models for evaluating drugs and vaccine candidates against malaria and other human diseases. The immune system of this animal model must be characterised to assess whether the results obtained here can be extrapolated to humans. Class I and II major histocompatibility complex (MHC) proteins are amongst the most important molecules involved in response to pathogens; in spite of this, the techniques available for genotyping these molecules are usually expensive and/or time-consuming. Previous studies have reported MHC-DRB class II gene typing by microsatellite in Old World primates and humans, showing that such technique provides a fast, reliable and effective alternative to the commonly used ones. Based on this information, a microsatellite present in MHC-DRB intron 2 and its evolutionary patterns were identified in two Aotus species (A. vociferans and A. nancymaae ), as well as its potential for genotyping class II MHC-DRB in these primates. © 2014 López et al.
publishDate 2014
dc.date.created.none.fl_str_mv 2014
dc.date.issued.none.fl_str_mv 2014-03-12
dc.date.accessioned.none.fl_str_mv 2018-11-19T15:46:28Z
dc.date.available.none.fl_str_mv 2018-11-19T15:46:28Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1371/journal.pone.0096973
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.uri.none.fl_str_mv http://repository.urosario.edu.co/handle/10336/18702
url https://doi.org/10.1371/journal.pone.0096973
http://repository.urosario.edu.co/handle/10336/18702
identifier_str_mv 1932-6203
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationIssue.none.fl_str_mv No. 5
dc.relation.citationTitle.none.fl_str_mv PLoS ONE
dc.relation.citationVolume.none.fl_str_mv Vol. 9
dc.relation.ispartof.spa.fl_str_mv PLoS ONE, ISSN: 1932-6203, Vol. 9/No. 5 (2014)
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dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
dc.rights.cc.spa.fl_str_mv https://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv Abierto (Texto Completo)
https://creativecommons.org/licenses/by/4.0/
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
institution Universidad del Rosario
dc.source.bibliographicCitation.spa.fl_str_mv Ward, J.M., Vallender, E.J., The resurgence and genetic implications of New World primates in biomedical research (2012) Trends Genet, 28, pp. 586-591
dc.source.instname.none.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.none.fl_str_mv reponame:Repositorio Institucional EdocUR
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spelling f04dd5c4-d685-4cee-a3d9-d2c759ef003f60085e2385a-174f-4c6b-87d7-def493015d27600a9524704-c54f-4bc2-ab89-fffd5be08b1d60010ecd4f9-843f-4ef2-bec0-7d39d3381a13600796530656002018-11-19T15:46:28Z2018-11-19T15:46:28Z20142014-03-12Non-human primates belonging to the Aotus genus have been shown to be excellent experimental models for evaluating drugs and vaccine candidates against malaria and other human diseases. The immune system of this animal model must be characterised to assess whether the results obtained here can be extrapolated to humans. Class I and II major histocompatibility complex (MHC) proteins are amongst the most important molecules involved in response to pathogens; in spite of this, the techniques available for genotyping these molecules are usually expensive and/or time-consuming. Previous studies have reported MHC-DRB class II gene typing by microsatellite in Old World primates and humans, showing that such technique provides a fast, reliable and effective alternative to the commonly used ones. Based on this information, a microsatellite present in MHC-DRB intron 2 and its evolutionary patterns were identified in two Aotus species (A. vociferans and A. nancymaae ), as well as its potential for genotyping class II MHC-DRB in these primates. © 2014 López et al.application/pdfhttps://doi.org/10.1371/journal.pone.00969731932-6203http://repository.urosario.edu.co/handle/10336/18702engNo. 5PLoS ONEVol. 9PLoS ONE, ISSN: 1932-6203, Vol. 9/No. 5 (2014)https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0096973&type=printableAbierto (Texto Completo)https://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2Ward, J.M., Vallender, E.J., The resurgence and genetic implications of New World primates in biomedical research (2012) Trends Genet, 28, pp. 586-591instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURADN complementarioAntígeno Hla DrAntígeno Hla Drb1Antígeno Hla Drb3Antígeno mayor de histocompatibilidad clase 1Antígeno mayor de histocompatibilidad clase 2Hla antígeno clase 2ADN microsatéliteAlelosCélula animalAotus VociferansEstudio controladoSecuencia de ADNExónAnálisis genéticoGenotipoTécnica de genotipadoIntrónMhc Drb GenMhc Drb1 GenMhc Drb3 GenMarcador de microsatéliteEvolución MolecularFilogenia molecularÁrbol filogenéticoOrnitorrincoAlineación de secuenciaRepetición corta en tándemAótidosRepeticiones de microsatélitesMicrosatellite RepeatsHistocompatibility Antigens Class IiAotidaeShort Tandem RepeatSequence AlignmentPlatyrrhiniPhylogenetic TreeMolecular PhylogenyMolecular EvolutionMicrosatellite MarkerMhc Drb3 GeneMhc Drb1 GeneMhc Drb GeneIntronGenotyping TechniqueGenotypeGenetic AnalysisExonDna SequenceControlled StudyAotus NancymaaeAnimal CellMicrosatellite DnaAlleleHla Antigen Class 2Major Histocompatibility Antigen Class 2Hla Drb3 AntigenMajor Histocompatibility Antigen Class 1Hla Drb1 AntigenHla Dr AntigenComplementary DnaAotus VociferansMalariaHistocompatibilidadExperimentación con animalesPrimatesCharacterising a microsatellite for DRB typing in Aotus vociferans and Aotus nancymaae (Platyrrhini)articleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501López, CarolinaSuárez, Carlos F.Cadavid, LuisPatarroyo, Manuel E.Patarroyo, Manuel A.López, CarolinaSuárez, Carlos F.Cadavid, Luis F.Patarroyo, Manuel E.Patarroyo, Manuel A.ORIGINAL114.pdfapplication/pdf1962180https://repository.urosario.edu.co/bitstreams/d52be696-1954-411e-9729-e5c117ab0235/downloada742e22e2cce3d512f1e30c7ad23854fMD51TEXT114.pdf.txt114.pdf.txtExtracted texttext/plain62850https://repository.urosario.edu.co/bitstreams/d9cbbf5e-6de4-41dd-8f00-17cf798cf0b4/download79780ec280a8aa095066e8b76a84bf4eMD52THUMBNAIL114.pdf.jpg114.pdf.jpgGenerated Thumbnailimage/jpeg4650https://repository.urosario.edu.co/bitstreams/fdb03f15-d462-4ffc-bd2d-60e6f01f6c7d/downloade5d705f9bbee5e5e0ff8cd2441af8d28MD5310336/18702oai:repository.urosario.edu.co:10336/187022022-08-29 10:16:50.322https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co