Genómica y transcriptómica comparativa en cepas de Leishmania de Colombia

La Leishmaniasis es una enfermedad tropical ocasionada por parásitos protozoarios del género Leishmania. En Colombia esta enfermedad representa un grave problema de salud pública debido al elevado número de casos reportados anualmente, la cantidad de especies encontradas y la aparición de cepas resi...

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Autores:
Tipo de recurso:
Fecha de publicación:
2019
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
spa
OAI Identifier:
oai:repository.urosario.edu.co:10336/20397
Acceso en línea:
https://doi.org/10.48713/10336_20397
https://repository.urosario.edu.co/handle/10336/20397
Palabra clave:
Leishmania
Resistencia
ADN-seq
ARN-seq
Glucantime
Somia
VNC
SNPs
Ciencias médicas, Medicina
Leishmaniasis
DNA-seq
RNA seq
CNV
SNPs
Resistance
Medicina
Enfermedades
Rights
License
Atribución-NoComercial-SinDerivadas 2.5 Colombia
id EDOCUR2_7ab4b87ba8382c81d13a67120d64cc18
oai_identifier_str oai:repository.urosario.edu.co:10336/20397
network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
dc.title.spa.fl_str_mv Genómica y transcriptómica comparativa en cepas de Leishmania de Colombia
dc.title.TranslatedTitle.eng.fl_str_mv Genomic and transcriptomic comparative in Leishmania species from Colombia
title Genómica y transcriptómica comparativa en cepas de Leishmania de Colombia
spellingShingle Genómica y transcriptómica comparativa en cepas de Leishmania de Colombia
Leishmania
Resistencia
ADN-seq
ARN-seq
Glucantime
Somia
VNC
SNPs
Ciencias médicas, Medicina
Leishmaniasis
DNA-seq
RNA seq
CNV
SNPs
Resistance
Medicina
Enfermedades
title_short Genómica y transcriptómica comparativa en cepas de Leishmania de Colombia
title_full Genómica y transcriptómica comparativa en cepas de Leishmania de Colombia
title_fullStr Genómica y transcriptómica comparativa en cepas de Leishmania de Colombia
title_full_unstemmed Genómica y transcriptómica comparativa en cepas de Leishmania de Colombia
title_sort Genómica y transcriptómica comparativa en cepas de Leishmania de Colombia
dc.contributor.advisor.none.fl_str_mv Pavia Velandia, Paula Ximena
Ramírez, Juan David
dc.subject.spa.fl_str_mv Leishmania
Resistencia
ADN-seq
ARN-seq
Glucantime
Somia
VNC
SNPs
topic Leishmania
Resistencia
ADN-seq
ARN-seq
Glucantime
Somia
VNC
SNPs
Ciencias médicas, Medicina
Leishmaniasis
DNA-seq
RNA seq
CNV
SNPs
Resistance
Medicina
Enfermedades
dc.subject.ddc.spa.fl_str_mv Ciencias médicas, Medicina
dc.subject.keyword.spa.fl_str_mv Leishmaniasis
DNA-seq
RNA seq
CNV
SNPs
Resistance
dc.subject.lemb.spa.fl_str_mv Medicina
Enfermedades
description La Leishmaniasis es una enfermedad tropical ocasionada por parásitos protozoarios del género Leishmania. En Colombia esta enfermedad representa un grave problema de salud pública debido al elevado número de casos reportados anualmente, la cantidad de especies encontradas y la aparición de cepas resistentes, principalmente frente a los medicamentos de primera línea: antimoniato N-metil glucamina (Glucantime®). Hasta el momento, varios estudios utilizando técnicas de secuenciación de última generación y basados en herramientas como las “omicas” han proporcionado información crucial con respecto a los mecanismos de resistencia y adaptación utilizados por estos parásitos frente a los antimoniales. Sin embargo, este conocimiento en especies de Leishmania del nuevo mundo son escasos, así como estudios que permitan describir la arquitectura genómica intra-especifica en estas especies. Teniendo en cuenta lo anterior, este estudio evaluó el comportamiento genómico y transcriptómico de las principales especies de Leishmania que circulan en Colombia bajo la presión al N-metil glucamina (Glucantime® (SbIII), así como la arquitectura genómica de estas especies, obtenidas a partir de aislamientos clínicos. Los resultados producto de esta tesis permitieron describir que (i) la distribución de las especies de Leishmania a nivel urbano/selvático es diferente, (ii) los cambios genómicos y transcriptómicos en respuesta a los antimoniales trivalentes es una característica compartida entre especies del viejo y del nuevo mundo y (iii) describir la baja variación estructural encontrada en Leishmania braziliensis y Leishmania panamensis. Finalmente, consideramos que estudios adicionales son necesarios con el propósito de seguir ampliando el conocimiento acerca de los mecanismos de adaptación de este parásito.
publishDate 2019
dc.date.accessioned.none.fl_str_mv 2019-10-07T12:32:03Z
dc.date.available.none.fl_str_mv 2019-10-07T12:32:03Z
dc.date.created.none.fl_str_mv 2019-09-26
dc.type.eng.fl_str_mv doctoralThesis
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_db06
dc.type.document.spa.fl_str_mv Tesis
dc.type.spa.spa.fl_str_mv Tesis de doctorado
dc.identifier.doi.none.fl_str_mv https://doi.org/10.48713/10336_20397
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/20397
url https://doi.org/10.48713/10336_20397
https://repository.urosario.edu.co/handle/10336/20397
dc.language.iso.spa.fl_str_mv spa
language spa
dc.rights.spa.fl_str_mv Atribución-NoComercial-SinDerivadas 2.5 Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
dc.rights.uri.none.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/co/
rights_invalid_str_mv Atribución-NoComercial-SinDerivadas 2.5 Colombia
Abierto (Texto Completo)
http://creativecommons.org/licenses/by-nc-nd/2.5/co/
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad del Rosario
dc.publisher.department.spa.fl_str_mv Facultad de Ciencias Naturales y Matemáticas
dc.publisher.program.spa.fl_str_mv Doctorado en Ciencias Biomédicas y Biológicas
institution Universidad del Rosario
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spelling Pavia Velandia, Paula Ximenaf21a13de-5c7d-4591-be9e-47a4a987b05a-1Ramírez, Juan David1011716118600Patiño, Luz H.Doctor en Ciencias Biomédicas y BiológicasFull timebb8af714-296a-4fac-ac7b-7eb715be6bad6002019-10-07T12:32:03Z2019-10-07T12:32:03Z2019-09-26La Leishmaniasis es una enfermedad tropical ocasionada por parásitos protozoarios del género Leishmania. En Colombia esta enfermedad representa un grave problema de salud pública debido al elevado número de casos reportados anualmente, la cantidad de especies encontradas y la aparición de cepas resistentes, principalmente frente a los medicamentos de primera línea: antimoniato N-metil glucamina (Glucantime®). Hasta el momento, varios estudios utilizando técnicas de secuenciación de última generación y basados en herramientas como las “omicas” han proporcionado información crucial con respecto a los mecanismos de resistencia y adaptación utilizados por estos parásitos frente a los antimoniales. Sin embargo, este conocimiento en especies de Leishmania del nuevo mundo son escasos, así como estudios que permitan describir la arquitectura genómica intra-especifica en estas especies. Teniendo en cuenta lo anterior, este estudio evaluó el comportamiento genómico y transcriptómico de las principales especies de Leishmania que circulan en Colombia bajo la presión al N-metil glucamina (Glucantime® (SbIII), así como la arquitectura genómica de estas especies, obtenidas a partir de aislamientos clínicos. Los resultados producto de esta tesis permitieron describir que (i) la distribución de las especies de Leishmania a nivel urbano/selvático es diferente, (ii) los cambios genómicos y transcriptómicos en respuesta a los antimoniales trivalentes es una característica compartida entre especies del viejo y del nuevo mundo y (iii) describir la baja variación estructural encontrada en Leishmania braziliensis y Leishmania panamensis. Finalmente, consideramos que estudios adicionales son necesarios con el propósito de seguir ampliando el conocimiento acerca de los mecanismos de adaptación de este parásito.Leishmaniasis is a tropical disease caused by protozoan parasites of the genus Leishmania. In Colombia, this disease represents a serious public health problem due to the high number of cases reported annually, the number of species found and the appearance of resistant strains, mainly compared to first-line drugs: N-methyl glucamine antimoniato (Glucantime®) . So far, several studies using next generation sequencing and tools based on “omicas” have provided crucial information regarding the resistance and adaptation mechanisms used by these parasites against antimonials. However, this knowledge in Leishmania species of the new world is scarce, as well as studies that allow to describe the intra-specific genomic architecture in these species. Taking into account the above, this study evaluated the genomic and transcriptomic behavior of the main Leishmania species that circulate in Colombia under the pressure of N-methyl glucamine (Glucantime® (SbIII), as well as the genomic architecture of these species, obtained at from clinical isolates. The results of this thesis allowed us to describe that (i) the distribution of Leishmania species at urban / jungle level is different, (ii) genomic and transcriptomic changes in response to trivalent antimonials is a characteristic shared between species of the old and of the new world and (iii) describe the low structural variation found in Leishmania braziliensis and Leishmania panamensis. Finally, we consider that additional studies are necessary in order to continue expanding the knowledge about the adaptation mechanisms of this parasite. Departamento administrativo de Ciencia, Tecnología e Innovación (Colciencias), dentro del marco del Programa Nacional para promover la formación en la investigación (convocatoria 647)Dirección Académica de la Universidad del RosarioDirección de Investigación e Innovación de la Universidad del Rosarioapplication/pdfhttps://doi.org/10.48713/10336_20397 https://repository.urosario.edu.co/handle/10336/20397spaUniversidad del RosarioFacultad de Ciencias Naturales y MatemáticasDoctorado en Ciencias Biomédicas y BiológicasAtribución-NoComercial-SinDerivadas 2.5 ColombiaAbierto (Texto Completo)PARGRAFO: En caso de presentarse cualquier reclamación o acción por parte de un tercero en cuanto a los derechos de autor sobre la obra en cuestión, EL AUTOR, asumirá toda la responsabilidad, y saldrá en defensa de los derechos aquí autorizados; para todos los efectos la universidad actúa como un tercero de buena fe.http://creativecommons.org/licenses/by-nc-nd/2.5/co/http://purl.org/coar/access_right/c_abf2Herwaldt BL: Leishmaniasis. 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Scientific reports 2017, 7:40804.Real F, Vidal RO, Carazzolle MF, Mondego JM, Costa GG, Herai RH, Wurtele M, de Carvalho LM, Carmona e Ferreira R, Mortara RA et al: The genome sequence of Leishmania (Leishmania) amazonensis: functional annotation and extended analysis of gene models. 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