Validation of Oxford nanopore sequencing for improved New World Leishmania species identification via analysis of 70-kDA heat shock protein

Leishmaniasis is a parasitic disease caused by obligate intracellular protozoa of the genus Leishmania. This infection is characterized by a wide range of clinical manifestations, with symptoms greatly dependent on the causal parasitic species. Here we present the design and application of a new 70-...

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Autores:
Tipo de recurso:
Fecha de publicación:
2023
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/42177
Acceso en línea:
https://repository.urosario.edu.co/handle/10336/42177
Palabra clave:
Leishmania
MinION sequencing
HSP70-Long
HSP70-Short
Cutaneous Leishmaniasis
Rights
License
Attribution-NonCommercial-ShareAlike 4.0 International
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dc.title.spa.fl_str_mv Validation of Oxford nanopore sequencing for improved New World Leishmania species identification via analysis of 70-kDA heat shock protein
title Validation of Oxford nanopore sequencing for improved New World Leishmania species identification via analysis of 70-kDA heat shock protein
spellingShingle Validation of Oxford nanopore sequencing for improved New World Leishmania species identification via analysis of 70-kDA heat shock protein
Leishmania
MinION sequencing
HSP70-Long
HSP70-Short
Cutaneous Leishmaniasis
title_short Validation of Oxford nanopore sequencing for improved New World Leishmania species identification via analysis of 70-kDA heat shock protein
title_full Validation of Oxford nanopore sequencing for improved New World Leishmania species identification via analysis of 70-kDA heat shock protein
title_fullStr Validation of Oxford nanopore sequencing for improved New World Leishmania species identification via analysis of 70-kDA heat shock protein
title_full_unstemmed Validation of Oxford nanopore sequencing for improved New World Leishmania species identification via analysis of 70-kDA heat shock protein
title_sort Validation of Oxford nanopore sequencing for improved New World Leishmania species identification via analysis of 70-kDA heat shock protein
dc.subject.spa.fl_str_mv Leishmania
MinION sequencing
HSP70-Long
HSP70-Short
Cutaneous Leishmaniasis
topic Leishmania
MinION sequencing
HSP70-Long
HSP70-Short
Cutaneous Leishmaniasis
description Leishmaniasis is a parasitic disease caused by obligate intracellular protozoa of the genus Leishmania. This infection is characterized by a wide range of clinical manifestations, with symptoms greatly dependent on the causal parasitic species. Here we present the design and application of a new 70-kDa heat shock protein gene (hsp70)-based marker of 771 bp (HSP70-Long). We evaluated its sensitivity, specificity and diagnostic performance employing an amplicon-based MinION™ DNA sequencing assay to identify different Leishmania species in clinical samples from humans and reservoirs with cutaneous leishmaniasis (CL) and visceral leishmaniasis (VL). We also conducted a comparative analysis between our novel marker and a previously published HSP70 marker known as HSP70-Short, which spans 330 bp.
publishDate 2023
dc.date.created.spa.fl_str_mv 2023-12-01
dc.date.issued.spa.fl_str_mv 2023
dc.date.accessioned.none.fl_str_mv 2024-01-31T18:36:10Z
dc.date.available.none.fl_str_mv 2024-01-31T18:36:10Z
dc.type.spa.fl_str_mv article
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dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.spa.fl_str_mv 10.1186/s13071-023-06073-9
dc.identifier.issn.spa.fl_str_mv 1756-3305
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/42177
identifier_str_mv 10.1186/s13071-023-06073-9
1756-3305
url https://repository.urosario.edu.co/handle/10336/42177
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.uri.spa.fl_str_mv https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-023-06073-9
dc.rights.spa.fl_str_mv Attribution-NonCommercial-ShareAlike 4.0 International
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dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
dc.rights.uri.spa.fl_str_mv http://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv Attribution-NonCommercial-ShareAlike 4.0 International
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dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad del Rosario
dc.source.spa.fl_str_mv Parasites and Vectors
institution Universidad del Rosario
dc.source.instname.spa.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.spa.fl_str_mv reponame:Repositorio Institucional EdocUR
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