Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites
Background: Plasmodium vivax is the most widespread malarial species, causing significant morbidity worldwide. Knowledge is limited regarding the molecular mechanism of invasion due to the lack of a continuous in vitro culture system for these species. Since protein-protein and host-cell interaction...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2018
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/20256
- Acceso en línea:
- http://repository.urosario.edu.co/handle/10336/20256
- Palabra clave:
- Complementary Dna
Msp1 Protein
Msp8 Protein
Plasmodium Vivax 12 Protein
Plasmodium Vivax 41 Protein
Rap1 Protein
Rbp1A Protein
Unclassified Drug
Amino Acid Sequence
Cell Organelle
Controlled Study
Merozoite
Molecular Cloning
Nonhuman
Nucleic Acid Programmable Protein Array
Plasmodium Vivax
Protein Analysis
Protein Assembly
Protein Expression
Protein Microarray
Protein Protein Interaction
Enfermedades
Article
Malaria
Plasmodium
Epidemiología
- Rights
- License
- Abierto (Texto Completo)
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Repositorio EdocUR - U. Rosario |
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|
dc.title.spa.fl_str_mv |
Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites |
title |
Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites |
spellingShingle |
Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites Complementary Dna Msp1 Protein Msp8 Protein Plasmodium Vivax 12 Protein Plasmodium Vivax 41 Protein Rap1 Protein Rbp1A Protein Unclassified Drug Amino Acid Sequence Cell Organelle Controlled Study Merozoite Molecular Cloning Nonhuman Nucleic Acid Programmable Protein Array Plasmodium Vivax Protein Analysis Protein Assembly Protein Expression Protein Microarray Protein Protein Interaction Enfermedades Article Malaria Plasmodium Epidemiología |
title_short |
Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites |
title_full |
Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites |
title_fullStr |
Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites |
title_full_unstemmed |
Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites |
title_sort |
Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites |
dc.subject.spa.fl_str_mv |
Complementary Dna Msp1 Protein Msp8 Protein Plasmodium Vivax 12 Protein Plasmodium Vivax 41 Protein Rap1 Protein Rbp1A Protein Unclassified Drug Amino Acid Sequence Cell Organelle Controlled Study Merozoite Molecular Cloning Nonhuman Nucleic Acid Programmable Protein Array Plasmodium Vivax Protein Analysis Protein Assembly Protein Expression Protein Microarray Protein Protein Interaction |
topic |
Complementary Dna Msp1 Protein Msp8 Protein Plasmodium Vivax 12 Protein Plasmodium Vivax 41 Protein Rap1 Protein Rbp1A Protein Unclassified Drug Amino Acid Sequence Cell Organelle Controlled Study Merozoite Molecular Cloning Nonhuman Nucleic Acid Programmable Protein Array Plasmodium Vivax Protein Analysis Protein Assembly Protein Expression Protein Microarray Protein Protein Interaction Enfermedades Article Malaria Plasmodium Epidemiología |
dc.subject.ddc.spa.fl_str_mv |
Enfermedades |
dc.subject.keyword.spa.fl_str_mv |
Article |
dc.subject.lemb.spa.fl_str_mv |
Malaria Plasmodium Epidemiología |
description |
Background: Plasmodium vivax is the most widespread malarial species, causing significant morbidity worldwide. Knowledge is limited regarding the molecular mechanism of invasion due to the lack of a continuous in vitro culture system for these species. Since protein-protein and host-cell interactions play an essential role in the microorganism’s invasion and replication, elucidating protein function during invasion is critical when developing more effective control methods. Nucleic acid programmable protein array (NAPPA) has thus become a suitable technology for studying protein-protein and host-protein interactions since producing proteins through the in vitro transcription/translation (IVTT) method overcomes most of the drawbacks encountered to date, such as heterologous protein production, stability and purification. Results: Twenty P. vivax proteins on merozoite surface or in secretory organelles were selected and successfully cloned using gateway technology. Most constructs were displayed in the array expressed in situ, using the IVTT method. The Pv12 protein was used as bait for evaluating array functionality and co-expressed with P. vivax cDNA display in the array. It was found that Pv12 interacted with Pv41 (as previously described), as well as PvMSP142kDa, PvRBP1a, PvMSP8 and PvRAP1. Conclusions: NAPPA is a high-performance technique enabling co-expression of bait and query in situ, thereby enabling interactions to be analysed rapidly and reproducibly. It offers a fresh alternative for studying protein-protein and ligand-receptor interactions regarding a parasite which is difficult to cultivate (i.e. P. vivax). © 2018 The Author(s). |
publishDate |
2018 |
dc.date.created.none.fl_str_mv |
2018 |
dc.date.issued.none.fl_str_mv |
2018 |
dc.date.accessioned.none.fl_str_mv |
2019-09-11T17:25:36Z |
dc.date.available.none.fl_str_mv |
2019-09-11T17:25:36Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.none.fl_str_mv |
10.1186/s12936-018-2414-2 |
dc.identifier.issn.none.fl_str_mv |
1475-2875 |
dc.identifier.uri.none.fl_str_mv |
http://repository.urosario.edu.co/handle/10336/20256 |
identifier_str_mv |
10.1186/s12936-018-2414-2 1475-2875 |
url |
http://repository.urosario.edu.co/handle/10336/20256 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.citationTitle.none.fl_str_mv |
Malaria Journal |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 17 |
dc.relation.ispartof.spa.fl_str_mv |
Malaria Journal, ISSN:1475-2875, Vol. 17 (2018) |
dc.relation.uri.spa.fl_str_mv |
https://malariajournal.biomedcentral.com/articles/10.1186/s12936-018-2414-2 |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.acceso.spa.fl_str_mv |
Abierto (Texto Completo) |
rights_invalid_str_mv |
Abierto (Texto Completo) http://purl.org/coar/access_right/c_abf2 |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
institution |
Universidad del Rosario |
dc.source.bibliographicCitation.spa.fl_str_mv |
Mueller, I., Galinski, M.R., Baird, J.K., Carlton, J.M., Kochar, D.K., Alonso, P.L., Key gaps in the knowledge of Plasmodium vivax, a neglected human malaria parasite (2009) Lancet Infect Dis, 9, pp. 555-566. , 19695492 |
dc.source.instname.none.fl_str_mv |
instname:Universidad del Rosario |
dc.source.reponame.none.fl_str_mv |
reponame:Repositorio Institucional EdocUR |
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eca483d3-a229-42c8-8a6d-3b5e6f002e5b60092138aaa-6055-466a-9ed9-872aac0519b060079613230600912255896002d34d5a2-f76a-49e9-9040-5b57a0fdf56f600e73aa667-2d69-4f85-b0e7-ccaa5e5d0bdc6009122558960079653065600a3b0ffee-3a7c-4b47-846e-edbe7e5a68736002019-09-11T17:25:36Z2019-09-11T17:25:36Z20182018Background: Plasmodium vivax is the most widespread malarial species, causing significant morbidity worldwide. Knowledge is limited regarding the molecular mechanism of invasion due to the lack of a continuous in vitro culture system for these species. Since protein-protein and host-cell interactions play an essential role in the microorganism’s invasion and replication, elucidating protein function during invasion is critical when developing more effective control methods. Nucleic acid programmable protein array (NAPPA) has thus become a suitable technology for studying protein-protein and host-protein interactions since producing proteins through the in vitro transcription/translation (IVTT) method overcomes most of the drawbacks encountered to date, such as heterologous protein production, stability and purification. Results: Twenty P. vivax proteins on merozoite surface or in secretory organelles were selected and successfully cloned using gateway technology. Most constructs were displayed in the array expressed in situ, using the IVTT method. The Pv12 protein was used as bait for evaluating array functionality and co-expressed with P. vivax cDNA display in the array. It was found that Pv12 interacted with Pv41 (as previously described), as well as PvMSP142kDa, PvRBP1a, PvMSP8 and PvRAP1. Conclusions: NAPPA is a high-performance technique enabling co-expression of bait and query in situ, thereby enabling interactions to be analysed rapidly and reproducibly. It offers a fresh alternative for studying protein-protein and ligand-receptor interactions regarding a parasite which is difficult to cultivate (i.e. P. vivax). © 2018 The Author(s).application/pdf10.1186/s12936-018-2414-21475-2875http://repository.urosario.edu.co/handle/10336/20256engMalaria JournalVol. 17Malaria Journal, ISSN:1475-2875, Vol. 17 (2018)https://malariajournal.biomedcentral.com/articles/10.1186/s12936-018-2414-2Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2Mueller, I., Galinski, M.R., Baird, J.K., Carlton, J.M., Kochar, D.K., Alonso, P.L., Key gaps in the knowledge of Plasmodium vivax, a neglected human malaria parasite (2009) Lancet Infect Dis, 9, pp. 555-566. , 19695492instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURComplementary DnaMsp1 ProteinMsp8 ProteinPlasmodium Vivax 12 ProteinPlasmodium Vivax 41 ProteinRap1 ProteinRbp1A ProteinUnclassified DrugAmino Acid SequenceCell OrganelleControlled StudyMerozoiteMolecular CloningNonhumanNucleic Acid Programmable Protein ArrayPlasmodium VivaxProtein AnalysisProtein AssemblyProtein ExpressionProtein MicroarrayProtein Protein InteractionEnfermedades616600ArticleMalariaPlasmodiumEpidemiologíaSelf-assembling functional programmable protein array for studying protein-protein interactions in malaria parasitesarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Arévalo-Pinzón, GabrielaGonzález-González, MaríaSuarez Martinez, Carlos FernandoCurtidor, HernandoCarabias-Sánchez, JavierMuro, AntonioLaBaer, JoshuaPatarroyo, Manuel A.Fuentes, ManuelArévalo‑Pinzón, GabrielaGonzález‑González, MaríaSuárez, Carlos FernandoCurtidor, HernandoCarabias‑Sánchez, JavierMuro, AntonioLaBaer, JoshuaPatarroyo, Manuel AlfonsoFuentes, ManuelORIGINAL28.pdfapplication/pdf2802625https://repository.urosario.edu.co/bitstreams/8a09dd59-1778-4df4-8ed2-a2e15440abed/download1350d0337f058b4461a902fe5c92bf75MD51TEXT28.pdf.txt28.pdf.txtExtracted texttext/plain65022https://repository.urosario.edu.co/bitstreams/2fcad401-7846-4297-883e-44dd06ec6881/downloadd859d9e96261092b73b2fe834cc8111cMD52THUMBNAIL28.pdf.jpg28.pdf.jpgGenerated Thumbnailimage/jpeg4860https://repository.urosario.edu.co/bitstreams/3037e55a-f0ce-4fcf-81b6-ac9e67fb7b32/download72a50eb4f6c618eb78dc354ed63b5eecMD5310336/20256oai:repository.urosario.edu.co:10336/202562019-09-19 07:38:03.190837https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |