Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites

Background: Plasmodium vivax is the most widespread malarial species, causing significant morbidity worldwide. Knowledge is limited regarding the molecular mechanism of invasion due to the lack of a continuous in vitro culture system for these species. Since protein-protein and host-cell interaction...

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Autores:
Tipo de recurso:
Fecha de publicación:
2018
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/20256
Acceso en línea:
http://repository.urosario.edu.co/handle/10336/20256
Palabra clave:
Complementary Dna
Msp1 Protein
Msp8 Protein
Plasmodium Vivax 12 Protein
Plasmodium Vivax 41 Protein
Rap1 Protein
Rbp1A Protein
Unclassified Drug
Amino Acid Sequence
Cell Organelle
Controlled Study
Merozoite
Molecular Cloning
Nonhuman
Nucleic Acid Programmable Protein Array
Plasmodium Vivax
Protein Analysis
Protein Assembly
Protein Expression
Protein Microarray
Protein Protein Interaction
Enfermedades
Article
Malaria
Plasmodium
Epidemiología
Rights
License
Abierto (Texto Completo)
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network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
dc.title.spa.fl_str_mv Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites
title Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites
spellingShingle Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites
Complementary Dna
Msp1 Protein
Msp8 Protein
Plasmodium Vivax 12 Protein
Plasmodium Vivax 41 Protein
Rap1 Protein
Rbp1A Protein
Unclassified Drug
Amino Acid Sequence
Cell Organelle
Controlled Study
Merozoite
Molecular Cloning
Nonhuman
Nucleic Acid Programmable Protein Array
Plasmodium Vivax
Protein Analysis
Protein Assembly
Protein Expression
Protein Microarray
Protein Protein Interaction
Enfermedades
Article
Malaria
Plasmodium
Epidemiología
title_short Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites
title_full Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites
title_fullStr Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites
title_full_unstemmed Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites
title_sort Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites
dc.subject.spa.fl_str_mv Complementary Dna
Msp1 Protein
Msp8 Protein
Plasmodium Vivax 12 Protein
Plasmodium Vivax 41 Protein
Rap1 Protein
Rbp1A Protein
Unclassified Drug
Amino Acid Sequence
Cell Organelle
Controlled Study
Merozoite
Molecular Cloning
Nonhuman
Nucleic Acid Programmable Protein Array
Plasmodium Vivax
Protein Analysis
Protein Assembly
Protein Expression
Protein Microarray
Protein Protein Interaction
topic Complementary Dna
Msp1 Protein
Msp8 Protein
Plasmodium Vivax 12 Protein
Plasmodium Vivax 41 Protein
Rap1 Protein
Rbp1A Protein
Unclassified Drug
Amino Acid Sequence
Cell Organelle
Controlled Study
Merozoite
Molecular Cloning
Nonhuman
Nucleic Acid Programmable Protein Array
Plasmodium Vivax
Protein Analysis
Protein Assembly
Protein Expression
Protein Microarray
Protein Protein Interaction
Enfermedades
Article
Malaria
Plasmodium
Epidemiología
dc.subject.ddc.spa.fl_str_mv Enfermedades
dc.subject.keyword.spa.fl_str_mv Article
dc.subject.lemb.spa.fl_str_mv Malaria
Plasmodium
Epidemiología
description Background: Plasmodium vivax is the most widespread malarial species, causing significant morbidity worldwide. Knowledge is limited regarding the molecular mechanism of invasion due to the lack of a continuous in vitro culture system for these species. Since protein-protein and host-cell interactions play an essential role in the microorganism’s invasion and replication, elucidating protein function during invasion is critical when developing more effective control methods. Nucleic acid programmable protein array (NAPPA) has thus become a suitable technology for studying protein-protein and host-protein interactions since producing proteins through the in vitro transcription/translation (IVTT) method overcomes most of the drawbacks encountered to date, such as heterologous protein production, stability and purification. Results: Twenty P. vivax proteins on merozoite surface or in secretory organelles were selected and successfully cloned using gateway technology. Most constructs were displayed in the array expressed in situ, using the IVTT method. The Pv12 protein was used as bait for evaluating array functionality and co-expressed with P. vivax cDNA display in the array. It was found that Pv12 interacted with Pv41 (as previously described), as well as PvMSP142kDa, PvRBP1a, PvMSP8 and PvRAP1. Conclusions: NAPPA is a high-performance technique enabling co-expression of bait and query in situ, thereby enabling interactions to be analysed rapidly and reproducibly. It offers a fresh alternative for studying protein-protein and ligand-receptor interactions regarding a parasite which is difficult to cultivate (i.e. P. vivax). © 2018 The Author(s).
publishDate 2018
dc.date.created.none.fl_str_mv 2018
dc.date.issued.none.fl_str_mv 2018
dc.date.accessioned.none.fl_str_mv 2019-09-11T17:25:36Z
dc.date.available.none.fl_str_mv 2019-09-11T17:25:36Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv 10.1186/s12936-018-2414-2
dc.identifier.issn.none.fl_str_mv 1475-2875
dc.identifier.uri.none.fl_str_mv http://repository.urosario.edu.co/handle/10336/20256
identifier_str_mv 10.1186/s12936-018-2414-2
1475-2875
url http://repository.urosario.edu.co/handle/10336/20256
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationTitle.none.fl_str_mv Malaria Journal
dc.relation.citationVolume.none.fl_str_mv Vol. 17
dc.relation.ispartof.spa.fl_str_mv Malaria Journal, ISSN:1475-2875, Vol. 17 (2018)
dc.relation.uri.spa.fl_str_mv https://malariajournal.biomedcentral.com/articles/10.1186/s12936-018-2414-2
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
rights_invalid_str_mv Abierto (Texto Completo)
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
institution Universidad del Rosario
dc.source.bibliographicCitation.spa.fl_str_mv Mueller, I., Galinski, M.R., Baird, J.K., Carlton, J.M., Kochar, D.K., Alonso, P.L., Key gaps in the knowledge of Plasmodium vivax, a neglected human malaria parasite (2009) Lancet Infect Dis, 9, pp. 555-566. , 19695492
dc.source.instname.none.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.none.fl_str_mv reponame:Repositorio Institucional EdocUR
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spelling eca483d3-a229-42c8-8a6d-3b5e6f002e5b60092138aaa-6055-466a-9ed9-872aac0519b060079613230600912255896002d34d5a2-f76a-49e9-9040-5b57a0fdf56f600e73aa667-2d69-4f85-b0e7-ccaa5e5d0bdc6009122558960079653065600a3b0ffee-3a7c-4b47-846e-edbe7e5a68736002019-09-11T17:25:36Z2019-09-11T17:25:36Z20182018Background: Plasmodium vivax is the most widespread malarial species, causing significant morbidity worldwide. Knowledge is limited regarding the molecular mechanism of invasion due to the lack of a continuous in vitro culture system for these species. Since protein-protein and host-cell interactions play an essential role in the microorganism’s invasion and replication, elucidating protein function during invasion is critical when developing more effective control methods. Nucleic acid programmable protein array (NAPPA) has thus become a suitable technology for studying protein-protein and host-protein interactions since producing proteins through the in vitro transcription/translation (IVTT) method overcomes most of the drawbacks encountered to date, such as heterologous protein production, stability and purification. Results: Twenty P. vivax proteins on merozoite surface or in secretory organelles were selected and successfully cloned using gateway technology. Most constructs were displayed in the array expressed in situ, using the IVTT method. The Pv12 protein was used as bait for evaluating array functionality and co-expressed with P. vivax cDNA display in the array. It was found that Pv12 interacted with Pv41 (as previously described), as well as PvMSP142kDa, PvRBP1a, PvMSP8 and PvRAP1. Conclusions: NAPPA is a high-performance technique enabling co-expression of bait and query in situ, thereby enabling interactions to be analysed rapidly and reproducibly. It offers a fresh alternative for studying protein-protein and ligand-receptor interactions regarding a parasite which is difficult to cultivate (i.e. P. vivax). © 2018 The Author(s).application/pdf10.1186/s12936-018-2414-21475-2875http://repository.urosario.edu.co/handle/10336/20256engMalaria JournalVol. 17Malaria Journal, ISSN:1475-2875, Vol. 17 (2018)https://malariajournal.biomedcentral.com/articles/10.1186/s12936-018-2414-2Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2Mueller, I., Galinski, M.R., Baird, J.K., Carlton, J.M., Kochar, D.K., Alonso, P.L., Key gaps in the knowledge of Plasmodium vivax, a neglected human malaria parasite (2009) Lancet Infect Dis, 9, pp. 555-566. , 19695492instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURComplementary DnaMsp1 ProteinMsp8 ProteinPlasmodium Vivax 12 ProteinPlasmodium Vivax 41 ProteinRap1 ProteinRbp1A ProteinUnclassified DrugAmino Acid SequenceCell OrganelleControlled StudyMerozoiteMolecular CloningNonhumanNucleic Acid Programmable Protein ArrayPlasmodium VivaxProtein AnalysisProtein AssemblyProtein ExpressionProtein MicroarrayProtein Protein InteractionEnfermedades616600ArticleMalariaPlasmodiumEpidemiologíaSelf-assembling functional programmable protein array for studying protein-protein interactions in malaria parasitesarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Arévalo-Pinzón, GabrielaGonzález-González, MaríaSuarez Martinez, Carlos FernandoCurtidor, HernandoCarabias-Sánchez, JavierMuro, AntonioLaBaer, JoshuaPatarroyo, Manuel A.Fuentes, ManuelArévalo‑Pinzón, GabrielaGonzález‑González, MaríaSuárez, Carlos FernandoCurtidor, HernandoCarabias‑Sánchez, JavierMuro, AntonioLaBaer, JoshuaPatarroyo, Manuel AlfonsoFuentes, ManuelORIGINAL28.pdfapplication/pdf2802625https://repository.urosario.edu.co/bitstreams/8a09dd59-1778-4df4-8ed2-a2e15440abed/download1350d0337f058b4461a902fe5c92bf75MD51TEXT28.pdf.txt28.pdf.txtExtracted texttext/plain65022https://repository.urosario.edu.co/bitstreams/2fcad401-7846-4297-883e-44dd06ec6881/downloadd859d9e96261092b73b2fe834cc8111cMD52THUMBNAIL28.pdf.jpg28.pdf.jpgGenerated Thumbnailimage/jpeg4860https://repository.urosario.edu.co/bitstreams/3037e55a-f0ce-4fcf-81b6-ac9e67fb7b32/download72a50eb4f6c618eb78dc354ed63b5eecMD5310336/20256oai:repository.urosario.edu.co:10336/202562019-09-19 07:38:03.190837https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co