Characterizing viral species in mosquitoes (Culicidae) in the Colombian Orinoco

Mosquitoes (Diptera: Culicidae) are primary vectors of arthropod-borne viruses (arboviruses) that pose signifcant public health threats. Recent advances in sequencing technology emphasize the importance of understanding the arboviruses and nsect-specifc viruses (ISVs) hosted by mosquitoes, collectiv...

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Fecha de publicación:
2023
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/42099
Acceso en línea:
https://repository.urosario.edu.co/handle/10336/42099
Palabra clave:
Viral species in mosquitoes
Virome
Metagenomic study
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Attribution-NonCommercial-ShareAlike 4.0 International
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network_acronym_str EDOCUR2
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dc.title.spa.fl_str_mv Characterizing viral species in mosquitoes (Culicidae) in the Colombian Orinoco
title Characterizing viral species in mosquitoes (Culicidae) in the Colombian Orinoco
spellingShingle Characterizing viral species in mosquitoes (Culicidae) in the Colombian Orinoco
Viral species in mosquitoes
Virome
Metagenomic study
title_short Characterizing viral species in mosquitoes (Culicidae) in the Colombian Orinoco
title_full Characterizing viral species in mosquitoes (Culicidae) in the Colombian Orinoco
title_fullStr Characterizing viral species in mosquitoes (Culicidae) in the Colombian Orinoco
title_full_unstemmed Characterizing viral species in mosquitoes (Culicidae) in the Colombian Orinoco
title_sort Characterizing viral species in mosquitoes (Culicidae) in the Colombian Orinoco
dc.subject.spa.fl_str_mv Viral species in mosquitoes
Virome
Metagenomic study
topic Viral species in mosquitoes
Virome
Metagenomic study
description Mosquitoes (Diptera: Culicidae) are primary vectors of arthropod-borne viruses (arboviruses) that pose signifcant public health threats. Recent advances in sequencing technology emphasize the importance of understanding the arboviruses and nsect-specifc viruses (ISVs) hosted by mosquitoes, collectively called the “virome”. Colombia, a tropical country with favorable conditions for the development and adaptation of multiple species of Culicidae, ofers a favorable scenario for the transmission of epidemiologically important arboviruses. However, entomovirological surveillance studies are scarce in rural areas of the country, where humans, mosquitoes, and animals (both domestic and wild) coexist, leading to a higher risk of transmission of zoonotic diseases to humans. Thus, our study aimed to perform a preliminary metagenomic analysis of the mosquitoes of special relevance to public health belonging to the genera Ochlerotatus, Culex, Limatus, Mansonia, Psorophora, and Sabethes, within a rural savanna ecosystem in the Colombian Orinoco. We employed third-generation sequencing technology (Oxford Nanopore Technologies; ONT) to describe the virome of mosquitoes samples. Our results revealed that the virome was primarily shaped by insectspecifc viruses (ISVs), with the Ifaviridae family being the most prevalent across all mosquito samples. Furthermore, we identifed a group of ISVs that were common in all mosquito species tested, displaying the highest relative abundance concerning other groups of viruses. Notably, Hanko ifavirus-1 was especially prevalent in Culex eknomios (88.4%) and Ochlerotatus serratus (88.0%). Additionally, other ISVs, such as Guadalupe mosquito virus (GMV), Hubei mosquito virus1 (HMV1), Uxmal virus, Tanay virus, Cordoba virus, and Castlerea virus (all belonging to the Negevirus genus), were found as common viral species among the mosquitoes, although in lower proportions. These initial fndings contribute to our nderstanding of ISVs within mosquito vectors of the Culicidae family in the Eastern Plains of Colombia. We recommend that future research explore deeper into ISV species shared among diverse vector species, and their potential interactions with arboviruses. In addition, we also showed the need for a thorough exploration of the infuence of local rural habitat conditions on the shape of the virome in mosquito vectors.
publishDate 2023
dc.date.created.spa.fl_str_mv 2023-12-01
dc.date.issued.spa.fl_str_mv 2023
dc.date.accessioned.none.fl_str_mv 2024-01-31T18:21:42Z
dc.date.available.none.fl_str_mv 2024-01-31T18:21:42Z
dc.type.spa.fl_str_mv article
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dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.spa.fl_str_mv 10.1038/s41598-023-49232-9
dc.identifier.issn.spa.fl_str_mv 2045-2322
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/42099
identifier_str_mv 10.1038/s41598-023-49232-9
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url https://repository.urosario.edu.co/handle/10336/42099
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.uri.spa.fl_str_mv https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10716246/pdf/41598_2023_Article_49232.pdf
dc.rights.spa.fl_str_mv Attribution-NonCommercial-ShareAlike 4.0 International
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dc.publisher.spa.fl_str_mv Universidad del Rosario
dc.source.spa.fl_str_mv Scientific Reports
institution Universidad del Rosario
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