Characterizing viral species in mosquitoes (Culicidae) in the Colombian Orinoco
Mosquitoes (Diptera: Culicidae) are primary vectors of arthropod-borne viruses (arboviruses) that pose signifcant public health threats. Recent advances in sequencing technology emphasize the importance of understanding the arboviruses and nsect-specifc viruses (ISVs) hosted by mosquitoes, collectiv...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2023
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/42099
- Acceso en línea:
- https://repository.urosario.edu.co/handle/10336/42099
- Palabra clave:
- Viral species in mosquitoes
Virome
Metagenomic study
- Rights
- License
- Attribution-NonCommercial-ShareAlike 4.0 International
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|
dc.title.spa.fl_str_mv |
Characterizing viral species in mosquitoes (Culicidae) in the Colombian Orinoco |
title |
Characterizing viral species in mosquitoes (Culicidae) in the Colombian Orinoco |
spellingShingle |
Characterizing viral species in mosquitoes (Culicidae) in the Colombian Orinoco Viral species in mosquitoes Virome Metagenomic study |
title_short |
Characterizing viral species in mosquitoes (Culicidae) in the Colombian Orinoco |
title_full |
Characterizing viral species in mosquitoes (Culicidae) in the Colombian Orinoco |
title_fullStr |
Characterizing viral species in mosquitoes (Culicidae) in the Colombian Orinoco |
title_full_unstemmed |
Characterizing viral species in mosquitoes (Culicidae) in the Colombian Orinoco |
title_sort |
Characterizing viral species in mosquitoes (Culicidae) in the Colombian Orinoco |
dc.subject.spa.fl_str_mv |
Viral species in mosquitoes Virome Metagenomic study |
topic |
Viral species in mosquitoes Virome Metagenomic study |
description |
Mosquitoes (Diptera: Culicidae) are primary vectors of arthropod-borne viruses (arboviruses) that pose signifcant public health threats. Recent advances in sequencing technology emphasize the importance of understanding the arboviruses and nsect-specifc viruses (ISVs) hosted by mosquitoes, collectively called the “virome”. Colombia, a tropical country with favorable conditions for the development and adaptation of multiple species of Culicidae, ofers a favorable scenario for the transmission of epidemiologically important arboviruses. However, entomovirological surveillance studies are scarce in rural areas of the country, where humans, mosquitoes, and animals (both domestic and wild) coexist, leading to a higher risk of transmission of zoonotic diseases to humans. Thus, our study aimed to perform a preliminary metagenomic analysis of the mosquitoes of special relevance to public health belonging to the genera Ochlerotatus, Culex, Limatus, Mansonia, Psorophora, and Sabethes, within a rural savanna ecosystem in the Colombian Orinoco. We employed third-generation sequencing technology (Oxford Nanopore Technologies; ONT) to describe the virome of mosquitoes samples. Our results revealed that the virome was primarily shaped by insectspecifc viruses (ISVs), with the Ifaviridae family being the most prevalent across all mosquito samples. Furthermore, we identifed a group of ISVs that were common in all mosquito species tested, displaying the highest relative abundance concerning other groups of viruses. Notably, Hanko ifavirus-1 was especially prevalent in Culex eknomios (88.4%) and Ochlerotatus serratus (88.0%). Additionally, other ISVs, such as Guadalupe mosquito virus (GMV), Hubei mosquito virus1 (HMV1), Uxmal virus, Tanay virus, Cordoba virus, and Castlerea virus (all belonging to the Negevirus genus), were found as common viral species among the mosquitoes, although in lower proportions. These initial fndings contribute to our nderstanding of ISVs within mosquito vectors of the Culicidae family in the Eastern Plains of Colombia. We recommend that future research explore deeper into ISV species shared among diverse vector species, and their potential interactions with arboviruses. In addition, we also showed the need for a thorough exploration of the infuence of local rural habitat conditions on the shape of the virome in mosquito vectors. |
publishDate |
2023 |
dc.date.created.spa.fl_str_mv |
2023-12-01 |
dc.date.issued.spa.fl_str_mv |
2023 |
dc.date.accessioned.none.fl_str_mv |
2024-01-31T18:21:42Z |
dc.date.available.none.fl_str_mv |
2024-01-31T18:21:42Z |
dc.type.spa.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.spa.fl_str_mv |
10.1038/s41598-023-49232-9 |
dc.identifier.issn.spa.fl_str_mv |
2045-2322 |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/42099 |
identifier_str_mv |
10.1038/s41598-023-49232-9 2045-2322 |
url |
https://repository.urosario.edu.co/handle/10336/42099 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.uri.spa.fl_str_mv |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10716246/pdf/41598_2023_Article_49232.pdf |
dc.rights.spa.fl_str_mv |
Attribution-NonCommercial-ShareAlike 4.0 International |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.acceso.spa.fl_str_mv |
Abierto (Texto Completo) |
dc.rights.uri.spa.fl_str_mv |
https://creativecommons.org/licenses/by/4.0/ |
rights_invalid_str_mv |
Attribution-NonCommercial-ShareAlike 4.0 International Abierto (Texto Completo) https://creativecommons.org/licenses/by/4.0/ http://purl.org/coar/access_right/c_abf2 |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Universidad del Rosario |
dc.source.spa.fl_str_mv |
Scientific Reports |
institution |
Universidad del Rosario |
dc.source.instname.spa.fl_str_mv |
instname:Universidad del Rosario |
dc.source.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional EdocUR |
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4e9a295b-a84c-4a61-8d2f-40ad823a34feaf64b3c9-3edc-4233-9dc4-c48d5372530c49f61fa5-b446-4c68-8531-7b775aeab8b90d24305f-f936-4f3b-9919-57ea5a1349feba4ee81e-e15f-4e11-a6e4-000d3acda6896fac5401-cfd3-4a47-bd30-267447b53799b86f4a3e-6288-4193-8bf3-6e2f05987b9b7dac049d-c747-4b91-849c-c2efae4da8246fc54d6b-dd0c-4577-b64c-62640c7cdb472024-01-31T18:21:42Z2024-01-31T18:21:42Z2023-12-012023Mosquitoes (Diptera: Culicidae) are primary vectors of arthropod-borne viruses (arboviruses) that pose signifcant public health threats. Recent advances in sequencing technology emphasize the importance of understanding the arboviruses and nsect-specifc viruses (ISVs) hosted by mosquitoes, collectively called the “virome”. Colombia, a tropical country with favorable conditions for the development and adaptation of multiple species of Culicidae, ofers a favorable scenario for the transmission of epidemiologically important arboviruses. However, entomovirological surveillance studies are scarce in rural areas of the country, where humans, mosquitoes, and animals (both domestic and wild) coexist, leading to a higher risk of transmission of zoonotic diseases to humans. Thus, our study aimed to perform a preliminary metagenomic analysis of the mosquitoes of special relevance to public health belonging to the genera Ochlerotatus, Culex, Limatus, Mansonia, Psorophora, and Sabethes, within a rural savanna ecosystem in the Colombian Orinoco. We employed third-generation sequencing technology (Oxford Nanopore Technologies; ONT) to describe the virome of mosquitoes samples. Our results revealed that the virome was primarily shaped by insectspecifc viruses (ISVs), with the Ifaviridae family being the most prevalent across all mosquito samples. Furthermore, we identifed a group of ISVs that were common in all mosquito species tested, displaying the highest relative abundance concerning other groups of viruses. Notably, Hanko ifavirus-1 was especially prevalent in Culex eknomios (88.4%) and Ochlerotatus serratus (88.0%). Additionally, other ISVs, such as Guadalupe mosquito virus (GMV), Hubei mosquito virus1 (HMV1), Uxmal virus, Tanay virus, Cordoba virus, and Castlerea virus (all belonging to the Negevirus genus), were found as common viral species among the mosquitoes, although in lower proportions. These initial fndings contribute to our nderstanding of ISVs within mosquito vectors of the Culicidae family in the Eastern Plains of Colombia. We recommend that future research explore deeper into ISV species shared among diverse vector species, and their potential interactions with arboviruses. In addition, we also showed the need for a thorough exploration of the infuence of local rural habitat conditions on the shape of the virome in mosquito vectors.application/pdf10.1038/s41598-023-49232-92045-2322https://repository.urosario.edu.co/handle/10336/42099engUniversidad del Rosariohttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10716246/pdf/41598_2023_Article_49232.pdfAttribution-NonCommercial-ShareAlike 4.0 InternationalAbierto (Texto Completo)https://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2Scientific Reportsinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURViral species in mosquitoesViromeMetagenomic studyCharacterizing viral species in mosquitoes (Culicidae) in the Colombian OrinocoarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Gómez, MarcelaMartínez, DavidPáez Triana, LuisaLuna, NicolásDe las Salas, Jorge LuisHernández, CarolinaZamora FlórezMuñoz, MarinaRamírez González, AlejandroORIGINALCharacterizing viral species.pdfapplication/pdf3029026https://repository.urosario.edu.co/bitstreams/4f5c8cb1-aeca-4ab4-ba74-fc303acaf164/download24733cecfcc2d620e5704f9b0fa28910MD51TEXTCharacterizing viral species.pdf.txtCharacterizing viral species.pdf.txtExtracted texttext/plain62218https://repository.urosario.edu.co/bitstreams/6ce58141-517c-4eb4-a8a8-b520afff7fab/download4ed99c62f152e72e39e5c4185b65b7d6MD52THUMBNAILCharacterizing viral species.pdf.jpgCharacterizing viral species.pdf.jpgGenerated Thumbnailimage/jpeg4861https://repository.urosario.edu.co/bitstreams/8e440896-b181-41db-a783-a5370d6bc8c0/download62f4fd2d9f8e246c609e0e3d471e7fe4MD5310336/42099oai:repository.urosario.edu.co:10336/420992024-02-01 03:06:26.328https://creativecommons.org/licenses/by/4.0/Attribution-NonCommercial-ShareAlike 4.0 Internationalhttps://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |