TCR-contacting residues orientation and HLA-DR?* binding preference determine long-lasting protective immunity against malaria

Fully-protective, long-lasting, immunological (FPLLI) memory against Plasmodium falciparum malaria regarding immune protection-inducing protein structures (IMPIPS) vaccinated into monkeys previously challenged and re-challenged 60 days later with a lethal Aotus monkey-adapted P. falciparum strain was found to be associated with preferential high binding capacity to HLA-DR?1* allelic molecules of the major histocompatibility class II (MHC-II), rather than HLA-DR?3*, ?4*, ?5* alleles. Complete PPIIL 3D structure, a longer distance (26.5 Å ± 1.5 Å) between residues perfectly fitting into HLA-DR?1*PBR pockets 1 and 9, a gauche? rotamer orientation in p8 TCR-contacting polar residue and a larger volume of polar p2 residues was also found. This data, in association with previously-described p3 and p7 apolar residues having gauche+ orientation to form a perfect MHC-II-peptide-TCR complex, determines the stereo-electronic and topochemical characteristics associated with FPLLI immunological memory. © 2016 Elsevier Inc.

• Fecha de publicación: 2016
• Institución: Universidad del Rosario
• Repositorio: Repositorio EdocUR - U. Rosario
• Idioma: eng
• OAI Identifier: oai:repository.urosario.edu.co:10336/23515
• Acceso en línea: https://doi.org/10.1016/j.bbrc.2016.06.115; https://repository.urosario.edu.co/handle/10336/23515
• Palabra clave: Binding protein; Hla dr antigen; Hla drb3 antigen; Hla drb4 antigen; Hla drb5 antigen; Immunogenic protection inducing peptide structure; Major histocompatibility antigen class 2; Malaria vaccine; Multiprotein complex; Parasite antibody; Quinine; Recombinant protein; T lymphocyte receptor; Unclassified drug; Hla dr antigen; Lymphocyte antigen receptor; Protein binding; Animal experiment; Animal model; Antibody response; Antibody titer; Antigen binding; Article; Binding affinity; Controlled study; Immunogenetics; Immunological memory; Malaria falciparum; Monkey model; Nonhuman; Parasitemia; Plasmodium falciparum; Priority journal; Provocation test; Animal; Aotus; Binding site; Chemistry; Immunological memory; Immunology; Innate immunity; Malaria; Structure activity relation; Animals; Aotus trivirgatus; Binding sites; Hla-dr beta-chains; Immunologic memory; Malaria; Plasmodium falciparum; Protein binding; Structure-activity relationship; Antimalarial-vaccine; Immunological memory; Mhc-ii; Rotamer-orientation; T-cell-receptor; innate; antigen; t-cell; Immunity; Receptors
• License: Abierto (Texto Completo)