Reticulocyte count in red-blood-cell units stored in AS-1

Background and objectives: Previous data that showed maintenance of reticulocyte percentage in whole blood stored in CPDA-1 have led to the assumption that reticulocyte maturation becomes arrested during refrigerated storage. However, reticulocyte behaviour in red-blood-cell units stored in additive...

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Tipo de recurso:
Fecha de publicación:
2013
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/23483
Acceso en línea:
https://doi.org/10.1111/vox.12011
https://repository.urosario.edu.co/handle/10336/23483
Palabra clave:
Thiazole orange
Article
Erythrocyte concentrate
Erythrocyte preservation
Flow cytometry
Fluorescence analysis
Hemolysis
Priority journal
Reticulocyte count
Blood banks
Blood preservation
Erythrocytes
Flow cytometry
Humans
Reticulocyte count
Reticulocytes
Blood bank
High-fluorescence reticulocytes
Red-blood-cell unit
Reticulocyte
Rights
License
Abierto (Texto Completo)
id EDOCUR2_724241b21ab63034ebcae4b6260b2a8d
oai_identifier_str oai:repository.urosario.edu.co:10336/23483
network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
spelling 52542438600849a66d8-61c2-4f40-a4b4-1d2f792602612020-05-26T00:02:24Z2020-05-26T00:02:24Z2013Background and objectives: Previous data that showed maintenance of reticulocyte percentage in whole blood stored in CPDA-1 have led to the assumption that reticulocyte maturation becomes arrested during refrigerated storage. However, reticulocyte behaviour in red-blood-cell units stored in additive solutions has not yet been studied. This study was thus aimed at determining reticulocyte count and reticulocyte subtypes in red-blood-cells units stored in AS-1. Materials and methods: Reticulocyte percentage and subtypes were determined by flow cytometry with thiazole orange in six red-blood-cells units stored in AS-1. Results: Reticulocyte count was 26·8 ± 4·6 × 109/l at week 0·5 and 8·2 ±2·9 × 109/l at week 6. Total haemolysis during storage was 0·19 ± 0·08%. High-fluorescence reticulocytes were 2·0 ± 3·2 × 109/l at week 0·5 and decreased by weeks 2, 4 and 6. Low-fluorescence reticulocytes were 22·1 ± 3·1 × 109/l at week 0·5 and decreased by weeks 4 and 6. Conclusion: A significant decrease in reticulocytes occurred during red-blood-cells units' storage in AS-1. Even if it were assumed that all of haemolysed cells during storage were reticulocytes, there are a number of them whose disappearance cannot be explained by this mechanism. Changes observed in reticulocyte subtypes suggest that they mature during storage. © 2013 International Society of Blood Transfusion.application/pdfhttps://doi.org/10.1111/vox.120111423041000429007https://repository.urosario.edu.co/handle/10336/23483eng336No. 4331Vox SanguinisVol. 104Vox Sanguinis, ISSN:14230410, 00429007, Vol.104, No.4 (2013); pp. 331-336https://www.scopus.com/inward/record.uri?eid=2-s2.0-84876728529&doi=10.1111%2fvox.12011&partnerID=40&md5=ae20a760349d573f4a2f552851f86a01Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURThiazole orangeArticleErythrocyte concentrateErythrocyte preservationFlow cytometryFluorescence analysisHemolysisPriority journalReticulocyte countBlood banksBlood preservationErythrocytesFlow cytometryHumansReticulocyte countReticulocytesBlood bankHigh-fluorescence reticulocytesRed-blood-cell unitReticulocyteReticulocyte count in red-blood-cell units stored in AS-1articleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Urbina Bonilla, Adriana del PilarPalomino, F.10336/23483oai:repository.urosario.edu.co:10336/234832022-05-02 07:37:17.51279https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Reticulocyte count in red-blood-cell units stored in AS-1
title Reticulocyte count in red-blood-cell units stored in AS-1
spellingShingle Reticulocyte count in red-blood-cell units stored in AS-1
Thiazole orange
Article
Erythrocyte concentrate
Erythrocyte preservation
Flow cytometry
Fluorescence analysis
Hemolysis
Priority journal
Reticulocyte count
Blood banks
Blood preservation
Erythrocytes
Flow cytometry
Humans
Reticulocyte count
Reticulocytes
Blood bank
High-fluorescence reticulocytes
Red-blood-cell unit
Reticulocyte
title_short Reticulocyte count in red-blood-cell units stored in AS-1
title_full Reticulocyte count in red-blood-cell units stored in AS-1
title_fullStr Reticulocyte count in red-blood-cell units stored in AS-1
title_full_unstemmed Reticulocyte count in red-blood-cell units stored in AS-1
title_sort Reticulocyte count in red-blood-cell units stored in AS-1
dc.subject.keyword.spa.fl_str_mv Thiazole orange
Article
Erythrocyte concentrate
Erythrocyte preservation
Flow cytometry
Fluorescence analysis
Hemolysis
Priority journal
Reticulocyte count
Blood banks
Blood preservation
Erythrocytes
Flow cytometry
Humans
Reticulocyte count
Reticulocytes
Blood bank
High-fluorescence reticulocytes
Red-blood-cell unit
Reticulocyte
topic Thiazole orange
Article
Erythrocyte concentrate
Erythrocyte preservation
Flow cytometry
Fluorescence analysis
Hemolysis
Priority journal
Reticulocyte count
Blood banks
Blood preservation
Erythrocytes
Flow cytometry
Humans
Reticulocyte count
Reticulocytes
Blood bank
High-fluorescence reticulocytes
Red-blood-cell unit
Reticulocyte
description Background and objectives: Previous data that showed maintenance of reticulocyte percentage in whole blood stored in CPDA-1 have led to the assumption that reticulocyte maturation becomes arrested during refrigerated storage. However, reticulocyte behaviour in red-blood-cell units stored in additive solutions has not yet been studied. This study was thus aimed at determining reticulocyte count and reticulocyte subtypes in red-blood-cells units stored in AS-1. Materials and methods: Reticulocyte percentage and subtypes were determined by flow cytometry with thiazole orange in six red-blood-cells units stored in AS-1. Results: Reticulocyte count was 26·8 ± 4·6 × 109/l at week 0·5 and 8·2 ±2·9 × 109/l at week 6. Total haemolysis during storage was 0·19 ± 0·08%. High-fluorescence reticulocytes were 2·0 ± 3·2 × 109/l at week 0·5 and decreased by weeks 2, 4 and 6. Low-fluorescence reticulocytes were 22·1 ± 3·1 × 109/l at week 0·5 and decreased by weeks 4 and 6. Conclusion: A significant decrease in reticulocytes occurred during red-blood-cells units' storage in AS-1. Even if it were assumed that all of haemolysed cells during storage were reticulocytes, there are a number of them whose disappearance cannot be explained by this mechanism. Changes observed in reticulocyte subtypes suggest that they mature during storage. © 2013 International Society of Blood Transfusion.
publishDate 2013
dc.date.created.spa.fl_str_mv 2013
dc.date.accessioned.none.fl_str_mv 2020-05-26T00:02:24Z
dc.date.available.none.fl_str_mv 2020-05-26T00:02:24Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1111/vox.12011
dc.identifier.issn.none.fl_str_mv 14230410
00429007
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/23483
url https://doi.org/10.1111/vox.12011
https://repository.urosario.edu.co/handle/10336/23483
identifier_str_mv 14230410
00429007
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 336
dc.relation.citationIssue.none.fl_str_mv No. 4
dc.relation.citationStartPage.none.fl_str_mv 331
dc.relation.citationTitle.none.fl_str_mv Vox Sanguinis
dc.relation.citationVolume.none.fl_str_mv Vol. 104
dc.relation.ispartof.spa.fl_str_mv Vox Sanguinis, ISSN:14230410, 00429007, Vol.104, No.4 (2013); pp. 331-336
dc.relation.uri.spa.fl_str_mv https://www.scopus.com/inward/record.uri?eid=2-s2.0-84876728529&doi=10.1111%2fvox.12011&partnerID=40&md5=ae20a760349d573f4a2f552851f86a01
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
rights_invalid_str_mv Abierto (Texto Completo)
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
institution Universidad del Rosario
dc.source.instname.spa.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.spa.fl_str_mv reponame:Repositorio Institucional EdocUR
repository.name.fl_str_mv Repositorio institucional EdocUR
repository.mail.fl_str_mv edocur@urosario.edu.co
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