Reticulocyte count in red-blood-cell units stored in AS-1
Background and objectives: Previous data that showed maintenance of reticulocyte percentage in whole blood stored in CPDA-1 have led to the assumption that reticulocyte maturation becomes arrested during refrigerated storage. However, reticulocyte behaviour in red-blood-cell units stored in additive...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2013
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/23483
- Acceso en línea:
- https://doi.org/10.1111/vox.12011
https://repository.urosario.edu.co/handle/10336/23483
- Palabra clave:
- Thiazole orange
Article
Erythrocyte concentrate
Erythrocyte preservation
Flow cytometry
Fluorescence analysis
Hemolysis
Priority journal
Reticulocyte count
Blood banks
Blood preservation
Erythrocytes
Flow cytometry
Humans
Reticulocyte count
Reticulocytes
Blood bank
High-fluorescence reticulocytes
Red-blood-cell unit
Reticulocyte
- Rights
- License
- Abierto (Texto Completo)
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EDOCUR2 |
network_name_str |
Repositorio EdocUR - U. Rosario |
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52542438600849a66d8-61c2-4f40-a4b4-1d2f792602612020-05-26T00:02:24Z2020-05-26T00:02:24Z2013Background and objectives: Previous data that showed maintenance of reticulocyte percentage in whole blood stored in CPDA-1 have led to the assumption that reticulocyte maturation becomes arrested during refrigerated storage. However, reticulocyte behaviour in red-blood-cell units stored in additive solutions has not yet been studied. This study was thus aimed at determining reticulocyte count and reticulocyte subtypes in red-blood-cells units stored in AS-1. Materials and methods: Reticulocyte percentage and subtypes were determined by flow cytometry with thiazole orange in six red-blood-cells units stored in AS-1. Results: Reticulocyte count was 26·8 ± 4·6 × 109/l at week 0·5 and 8·2 ±2·9 × 109/l at week 6. Total haemolysis during storage was 0·19 ± 0·08%. High-fluorescence reticulocytes were 2·0 ± 3·2 × 109/l at week 0·5 and decreased by weeks 2, 4 and 6. Low-fluorescence reticulocytes were 22·1 ± 3·1 × 109/l at week 0·5 and decreased by weeks 4 and 6. Conclusion: A significant decrease in reticulocytes occurred during red-blood-cells units' storage in AS-1. Even if it were assumed that all of haemolysed cells during storage were reticulocytes, there are a number of them whose disappearance cannot be explained by this mechanism. Changes observed in reticulocyte subtypes suggest that they mature during storage. © 2013 International Society of Blood Transfusion.application/pdfhttps://doi.org/10.1111/vox.120111423041000429007https://repository.urosario.edu.co/handle/10336/23483eng336No. 4331Vox SanguinisVol. 104Vox Sanguinis, ISSN:14230410, 00429007, Vol.104, No.4 (2013); pp. 331-336https://www.scopus.com/inward/record.uri?eid=2-s2.0-84876728529&doi=10.1111%2fvox.12011&partnerID=40&md5=ae20a760349d573f4a2f552851f86a01Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURThiazole orangeArticleErythrocyte concentrateErythrocyte preservationFlow cytometryFluorescence analysisHemolysisPriority journalReticulocyte countBlood banksBlood preservationErythrocytesFlow cytometryHumansReticulocyte countReticulocytesBlood bankHigh-fluorescence reticulocytesRed-blood-cell unitReticulocyteReticulocyte count in red-blood-cell units stored in AS-1articleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Urbina Bonilla, Adriana del PilarPalomino, F.10336/23483oai:repository.urosario.edu.co:10336/234832022-05-02 07:37:17.51279https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |
dc.title.spa.fl_str_mv |
Reticulocyte count in red-blood-cell units stored in AS-1 |
title |
Reticulocyte count in red-blood-cell units stored in AS-1 |
spellingShingle |
Reticulocyte count in red-blood-cell units stored in AS-1 Thiazole orange Article Erythrocyte concentrate Erythrocyte preservation Flow cytometry Fluorescence analysis Hemolysis Priority journal Reticulocyte count Blood banks Blood preservation Erythrocytes Flow cytometry Humans Reticulocyte count Reticulocytes Blood bank High-fluorescence reticulocytes Red-blood-cell unit Reticulocyte |
title_short |
Reticulocyte count in red-blood-cell units stored in AS-1 |
title_full |
Reticulocyte count in red-blood-cell units stored in AS-1 |
title_fullStr |
Reticulocyte count in red-blood-cell units stored in AS-1 |
title_full_unstemmed |
Reticulocyte count in red-blood-cell units stored in AS-1 |
title_sort |
Reticulocyte count in red-blood-cell units stored in AS-1 |
dc.subject.keyword.spa.fl_str_mv |
Thiazole orange Article Erythrocyte concentrate Erythrocyte preservation Flow cytometry Fluorescence analysis Hemolysis Priority journal Reticulocyte count Blood banks Blood preservation Erythrocytes Flow cytometry Humans Reticulocyte count Reticulocytes Blood bank High-fluorescence reticulocytes Red-blood-cell unit Reticulocyte |
topic |
Thiazole orange Article Erythrocyte concentrate Erythrocyte preservation Flow cytometry Fluorescence analysis Hemolysis Priority journal Reticulocyte count Blood banks Blood preservation Erythrocytes Flow cytometry Humans Reticulocyte count Reticulocytes Blood bank High-fluorescence reticulocytes Red-blood-cell unit Reticulocyte |
description |
Background and objectives: Previous data that showed maintenance of reticulocyte percentage in whole blood stored in CPDA-1 have led to the assumption that reticulocyte maturation becomes arrested during refrigerated storage. However, reticulocyte behaviour in red-blood-cell units stored in additive solutions has not yet been studied. This study was thus aimed at determining reticulocyte count and reticulocyte subtypes in red-blood-cells units stored in AS-1. Materials and methods: Reticulocyte percentage and subtypes were determined by flow cytometry with thiazole orange in six red-blood-cells units stored in AS-1. Results: Reticulocyte count was 26·8 ± 4·6 × 109/l at week 0·5 and 8·2 ±2·9 × 109/l at week 6. Total haemolysis during storage was 0·19 ± 0·08%. High-fluorescence reticulocytes were 2·0 ± 3·2 × 109/l at week 0·5 and decreased by weeks 2, 4 and 6. Low-fluorescence reticulocytes were 22·1 ± 3·1 × 109/l at week 0·5 and decreased by weeks 4 and 6. Conclusion: A significant decrease in reticulocytes occurred during red-blood-cells units' storage in AS-1. Even if it were assumed that all of haemolysed cells during storage were reticulocytes, there are a number of them whose disappearance cannot be explained by this mechanism. Changes observed in reticulocyte subtypes suggest that they mature during storage. © 2013 International Society of Blood Transfusion. |
publishDate |
2013 |
dc.date.created.spa.fl_str_mv |
2013 |
dc.date.accessioned.none.fl_str_mv |
2020-05-26T00:02:24Z |
dc.date.available.none.fl_str_mv |
2020-05-26T00:02:24Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1111/vox.12011 |
dc.identifier.issn.none.fl_str_mv |
14230410 00429007 |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/23483 |
url |
https://doi.org/10.1111/vox.12011 https://repository.urosario.edu.co/handle/10336/23483 |
identifier_str_mv |
14230410 00429007 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.citationEndPage.none.fl_str_mv |
336 |
dc.relation.citationIssue.none.fl_str_mv |
No. 4 |
dc.relation.citationStartPage.none.fl_str_mv |
331 |
dc.relation.citationTitle.none.fl_str_mv |
Vox Sanguinis |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 104 |
dc.relation.ispartof.spa.fl_str_mv |
Vox Sanguinis, ISSN:14230410, 00429007, Vol.104, No.4 (2013); pp. 331-336 |
dc.relation.uri.spa.fl_str_mv |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84876728529&doi=10.1111%2fvox.12011&partnerID=40&md5=ae20a760349d573f4a2f552851f86a01 |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.acceso.spa.fl_str_mv |
Abierto (Texto Completo) |
rights_invalid_str_mv |
Abierto (Texto Completo) http://purl.org/coar/access_right/c_abf2 |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
institution |
Universidad del Rosario |
dc.source.instname.spa.fl_str_mv |
instname:Universidad del Rosario |
dc.source.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional EdocUR |
repository.name.fl_str_mv |
Repositorio institucional EdocUR |
repository.mail.fl_str_mv |
edocur@urosario.edu.co |
_version_ |
1814167687624916992 |