Reticulocyte count in red-blood-cell units stored in AS-1

Background and objectives: Previous data that showed maintenance of reticulocyte percentage in whole blood stored in CPDA-1 have led to the assumption that reticulocyte maturation becomes arrested during refrigerated storage. However, reticulocyte behaviour in red-blood-cell units stored in additive...

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Autores:
Tipo de recurso:
Fecha de publicación:
2013
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/23483
Acceso en línea:
https://doi.org/10.1111/vox.12011
https://repository.urosario.edu.co/handle/10336/23483
Palabra clave:
Thiazole orange
Article
Erythrocyte concentrate
Erythrocyte preservation
Flow cytometry
Fluorescence analysis
Hemolysis
Priority journal
Reticulocyte count
Blood banks
Blood preservation
Erythrocytes
Flow cytometry
Humans
Reticulocyte count
Reticulocytes
Blood bank
High-fluorescence reticulocytes
Red-blood-cell unit
Reticulocyte
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License
Abierto (Texto Completo)
Description
Summary:Background and objectives: Previous data that showed maintenance of reticulocyte percentage in whole blood stored in CPDA-1 have led to the assumption that reticulocyte maturation becomes arrested during refrigerated storage. However, reticulocyte behaviour in red-blood-cell units stored in additive solutions has not yet been studied. This study was thus aimed at determining reticulocyte count and reticulocyte subtypes in red-blood-cells units stored in AS-1. Materials and methods: Reticulocyte percentage and subtypes were determined by flow cytometry with thiazole orange in six red-blood-cells units stored in AS-1. Results: Reticulocyte count was 26·8 ± 4·6 × 109/l at week 0·5 and 8·2 ±2·9 × 109/l at week 6. Total haemolysis during storage was 0·19 ± 0·08%. High-fluorescence reticulocytes were 2·0 ± 3·2 × 109/l at week 0·5 and decreased by weeks 2, 4 and 6. Low-fluorescence reticulocytes were 22·1 ± 3·1 × 109/l at week 0·5 and decreased by weeks 4 and 6. Conclusion: A significant decrease in reticulocytes occurred during red-blood-cells units' storage in AS-1. Even if it were assumed that all of haemolysed cells during storage were reticulocytes, there are a number of them whose disappearance cannot be explained by this mechanism. Changes observed in reticulocyte subtypes suggest that they mature during storage. © 2013 International Society of Blood Transfusion.