IMPIPS : The Immune Protection-Inducing Protein Structure concept in the search for steric-electron and topochemical principles for complete fully-protective chemically synthesised vaccine development

Determining immune protection-inducing protein structures (IMPIPS) involves defining the stereo-electron and topochemical characteristics which are essential in MHC-p-TCR complex formation. Modified high activity binding peptides (mHABP) were thus synthesised to produce a large panel of IMPIPS measu...

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Autores:
Tipo de recurso:
Fecha de publicación:
2015
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/19030
Acceso en línea:
https://doi.org/10.1371/journal.pone.0123249
http://repository.urosario.edu.co/handle/10336/19030
Palabra clave:
Proteína de unión
epítopo
Antígeno Hla Dr
Hidrógeno
Vacuna contra la malaria
Nitrógeno
Oxígeno
Prolina
Cloruro de sodio
Péptido sintético
Vacuna recombinante
Animal Experiment
Modelo animal
Reacción de anticuerpo de antígeno
Aoto
Sitio de unión
Estudio controlado
Estructura de la droga
Electrón
Enlace de hidrógeno
Estructura proteica inductora de protección inmunitaria
Respuesta inmune
inmunoensayo
inmunogenicidad
Malaria
No humano
Virulencia del parásito
Síntesis de péptidos
Química Física
Plasmodium falciparum
Enlace proteico
Estructura de la proteína
Animal
Chemistry
Haplorhin
Conformación de proteínas
Biología
Synthetic
Vaccines
Proline
Synthetic Peptide
Sodium Chloride
Recombinant Vaccine
Animal Model
Animal Experiment
Antigen Antibody Reaction
Aotus
Binding Site
Controlled Study
Drug Structure
Electron
Hydrogen Bond
Immune Protection Inducing Protein
Structure
Immune Response
Immunoassay
Binding Protein
Epitope
Hla Dr Antigen
Hydrogen
Malaria Vaccine
Oxygen
Immunogenicity
Nonhuman
Parasite Virulence
Peptide Synthesis
Physical Chemistry
Plasmodium Falciparum
Protein Binding
Protein Structure
Chemistry
Protein Conformation
Haplorhini
Animals
Electrons
Nitrogen
Inmunología
Proteínas portadoras
Antígenos
Rights
License
Abierto (Texto Completo)
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network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
dc.title.spa.fl_str_mv IMPIPS : The Immune Protection-Inducing Protein Structure concept in the search for steric-electron and topochemical principles for complete fully-protective chemically synthesised vaccine development
title IMPIPS : The Immune Protection-Inducing Protein Structure concept in the search for steric-electron and topochemical principles for complete fully-protective chemically synthesised vaccine development
spellingShingle IMPIPS : The Immune Protection-Inducing Protein Structure concept in the search for steric-electron and topochemical principles for complete fully-protective chemically synthesised vaccine development
Proteína de unión
epítopo
Antígeno Hla Dr
Hidrógeno
Vacuna contra la malaria
Nitrógeno
Oxígeno
Prolina
Cloruro de sodio
Péptido sintético
Vacuna recombinante
Animal Experiment
Modelo animal
Reacción de anticuerpo de antígeno
Aoto
Sitio de unión
Estudio controlado
Estructura de la droga
Electrón
Enlace de hidrógeno
Estructura proteica inductora de protección inmunitaria
Respuesta inmune
inmunoensayo
inmunogenicidad
Malaria
No humano
Virulencia del parásito
Síntesis de péptidos
Química Física
Plasmodium falciparum
Enlace proteico
Estructura de la proteína
Animal
Chemistry
Haplorhin
Conformación de proteínas
Biología
Synthetic
Vaccines
Proline
Synthetic Peptide
Sodium Chloride
Recombinant Vaccine
Animal Model
Animal Experiment
Antigen Antibody Reaction
Aotus
Binding Site
Controlled Study
Drug Structure
Electron
Hydrogen Bond
Immune Protection Inducing Protein
Structure
Immune Response
Immunoassay
Binding Protein
Epitope
Hla Dr Antigen
Hydrogen
Malaria Vaccine
Oxygen
Immunogenicity
Nonhuman
Parasite Virulence
Peptide Synthesis
Physical Chemistry
Plasmodium Falciparum
Protein Binding
Protein Structure
Chemistry
Protein Conformation
Haplorhini
Animals
Electrons
Nitrogen
Inmunología
Proteínas portadoras
Antígenos
title_short IMPIPS : The Immune Protection-Inducing Protein Structure concept in the search for steric-electron and topochemical principles for complete fully-protective chemically synthesised vaccine development
title_full IMPIPS : The Immune Protection-Inducing Protein Structure concept in the search for steric-electron and topochemical principles for complete fully-protective chemically synthesised vaccine development
title_fullStr IMPIPS : The Immune Protection-Inducing Protein Structure concept in the search for steric-electron and topochemical principles for complete fully-protective chemically synthesised vaccine development
title_full_unstemmed IMPIPS : The Immune Protection-Inducing Protein Structure concept in the search for steric-electron and topochemical principles for complete fully-protective chemically synthesised vaccine development
title_sort IMPIPS : The Immune Protection-Inducing Protein Structure concept in the search for steric-electron and topochemical principles for complete fully-protective chemically synthesised vaccine development
dc.subject.spa.fl_str_mv Proteína de unión
epítopo
Antígeno Hla Dr
Hidrógeno
Vacuna contra la malaria
Nitrógeno
Oxígeno
Prolina
Cloruro de sodio
Péptido sintético
Vacuna recombinante
Animal Experiment
Modelo animal
Reacción de anticuerpo de antígeno
Aoto
Sitio de unión
Estudio controlado
Estructura de la droga
Electrón
Enlace de hidrógeno
Estructura proteica inductora de protección inmunitaria
Respuesta inmune
inmunoensayo
inmunogenicidad
Malaria
No humano
Virulencia del parásito
Síntesis de péptidos
Química Física
Plasmodium falciparum
Enlace proteico
Estructura de la proteína
Animal
Chemistry
Haplorhin
Conformación de proteínas
topic Proteína de unión
epítopo
Antígeno Hla Dr
Hidrógeno
Vacuna contra la malaria
Nitrógeno
Oxígeno
Prolina
Cloruro de sodio
Péptido sintético
Vacuna recombinante
Animal Experiment
Modelo animal
Reacción de anticuerpo de antígeno
Aoto
Sitio de unión
Estudio controlado
Estructura de la droga
Electrón
Enlace de hidrógeno
Estructura proteica inductora de protección inmunitaria
Respuesta inmune
inmunoensayo
inmunogenicidad
Malaria
No humano
Virulencia del parásito
Síntesis de péptidos
Química Física
Plasmodium falciparum
Enlace proteico
Estructura de la proteína
Animal
Chemistry
Haplorhin
Conformación de proteínas
Biología
Synthetic
Vaccines
Proline
Synthetic Peptide
Sodium Chloride
Recombinant Vaccine
Animal Model
Animal Experiment
Antigen Antibody Reaction
Aotus
Binding Site
Controlled Study
Drug Structure
Electron
Hydrogen Bond
Immune Protection Inducing Protein
Structure
Immune Response
Immunoassay
Binding Protein
Epitope
Hla Dr Antigen
Hydrogen
Malaria Vaccine
Oxygen
Immunogenicity
Nonhuman
Parasite Virulence
Peptide Synthesis
Physical Chemistry
Plasmodium Falciparum
Protein Binding
Protein Structure
Chemistry
Protein Conformation
Haplorhini
Animals
Electrons
Nitrogen
Inmunología
Proteínas portadoras
Antígenos
dc.subject.ddc.spa.fl_str_mv Biología
dc.subject.keyword.eng.fl_str_mv Synthetic
Vaccines
Proline
Synthetic Peptide
Sodium Chloride
Recombinant Vaccine
Animal Model
Animal Experiment
Antigen Antibody Reaction
Aotus
Binding Site
Controlled Study
Drug Structure
Electron
Hydrogen Bond
Immune Protection Inducing Protein
Structure
Immune Response
Immunoassay
Binding Protein
Epitope
Hla Dr Antigen
Hydrogen
Malaria Vaccine
Oxygen
Immunogenicity
Nonhuman
Parasite Virulence
Peptide Synthesis
Physical Chemistry
Plasmodium Falciparum
Protein Binding
Protein Structure
Chemistry
Protein Conformation
Haplorhini
Animals
Electrons
dc.subject.keyword.spa.fl_str_mv Nitrogen
dc.subject.lemb.spa.fl_str_mv Inmunología
Proteínas portadoras
Antígenos
description Determining immune protection-inducing protein structures (IMPIPS) involves defining the stereo-electron and topochemical characteristics which are essential in MHC-p-TCR complex formation. Modified high activity binding peptides (mHABP) were thus synthesised to produce a large panel of IMPIPS measuring 26.5 ±3.5Å between the farthest atoms fitting into Pockets 1 to 9 of HLA-DRβ1∗structures. They displayed a polyproline II-like (PPIIL) structure with their backbone O and N atoms orientated to establish H-bonds with specific residues from HLA-DRβ∗-peptide binding regions (PBR). Residues having specific charge and gauche+ orientation regarding p3χ1, p5χ2, and p7χ1 angles determined appropriate rotamer orientation for perfectly fitting into the TCR to induce an appropriate immune response. Immunological assays in Aotus monkeys involving IMPIPS mixtures led to promising results; taken together with the aforementioned physicochemical principles, noninterfering, long-lasting, protection-inducing, multi-epitope, multistage, minimal subunit-based chemically-synthesised peptides can be designed against diseases scourging humankind. © 2015 Patarroyo et al.
publishDate 2015
dc.date.created.none.fl_str_mv 2015
dc.date.issued.none.fl_str_mv 2015-04-16
dc.date.accessioned.none.fl_str_mv 2019-02-08T19:45:56Z
dc.date.available.none.fl_str_mv 2019-02-08T19:45:56Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1371/journal.pone.0123249
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.uri.none.fl_str_mv http://repository.urosario.edu.co/handle/10336/19030
url https://doi.org/10.1371/journal.pone.0123249
http://repository.urosario.edu.co/handle/10336/19030
identifier_str_mv 1932-6203
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationIssue.none.fl_str_mv No. 4
dc.relation.citationTitle.none.fl_str_mv PLoS ONE
dc.relation.citationVolume.none.fl_str_mv Vol. 10
dc.relation.ispartof.spa.fl_str_mv PLoS ONE, ISSN: 1932-6203, Vol. 10/No. 4 (2015)
dc.relation.uri.spa.fl_str_mv https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0123249&type=printable
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
dc.rights.cc.spa.fl_str_mv https://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv Abierto (Texto Completo)
https://creativecommons.org/licenses/by/4.0/
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
institution Universidad del Rosario
dc.source.bibliographicCitation.spa.fl_str_mv Murray, C.J., Rosenfeld, L.C., Lim, S.S., Andrews, K.G., Foreman, K.J., Haring, D., Global malaria mortality between 1980 and 2010: A systematic analysis (2012) Lancet, 379, pp. 413-431. , PMID: 22305225
dc.source.instname.none.fl_str_mv instname:Universidad del Rosario
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spelling 9e3ba9df-fe89-48fe-9521-cc8f452d56f560051890881600e6f90bd2-8cc5-4a2b-ae26-6d77808b3ce66005172101860001a1c86e-e857-46fd-8fa7-3664f825e9ba6006884bc3a-3c67-485f-989c-93c12b92025d600796530656002019-02-08T19:45:56Z2019-02-08T19:45:56Z20152015-04-16Determining immune protection-inducing protein structures (IMPIPS) involves defining the stereo-electron and topochemical characteristics which are essential in MHC-p-TCR complex formation. Modified high activity binding peptides (mHABP) were thus synthesised to produce a large panel of IMPIPS measuring 26.5 ±3.5Å between the farthest atoms fitting into Pockets 1 to 9 of HLA-DRβ1∗structures. They displayed a polyproline II-like (PPIIL) structure with their backbone O and N atoms orientated to establish H-bonds with specific residues from HLA-DRβ∗-peptide binding regions (PBR). Residues having specific charge and gauche+ orientation regarding p3χ1, p5χ2, and p7χ1 angles determined appropriate rotamer orientation for perfectly fitting into the TCR to induce an appropriate immune response. Immunological assays in Aotus monkeys involving IMPIPS mixtures led to promising results; taken together with the aforementioned physicochemical principles, noninterfering, long-lasting, protection-inducing, multi-epitope, multistage, minimal subunit-based chemically-synthesised peptides can be designed against diseases scourging humankind. © 2015 Patarroyo et al.application/pdfhttps://doi.org/10.1371/journal.pone.01232491932-6203http://repository.urosario.edu.co/handle/10336/19030engNo. 4PLoS ONEVol. 10PLoS ONE, ISSN: 1932-6203, Vol. 10/No. 4 (2015)https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0123249&type=printableAbierto (Texto Completo)https://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2Murray, C.J., Rosenfeld, L.C., Lim, S.S., Andrews, K.G., Foreman, K.J., Haring, D., Global malaria mortality between 1980 and 2010: A systematic analysis (2012) Lancet, 379, pp. 413-431. , PMID: 22305225instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURProteína de uniónepítopoAntígeno Hla DrHidrógenoVacuna contra la malariaNitrógenoOxígenoProlinaCloruro de sodioPéptido sintéticoVacuna recombinanteAnimal ExperimentModelo animalReacción de anticuerpo de antígenoAotoSitio de uniónEstudio controladoEstructura de la drogaElectrónEnlace de hidrógenoEstructura proteica inductora de protección inmunitariaRespuesta inmuneinmunoensayoinmunogenicidadMalariaNo humanoVirulencia del parásitoSíntesis de péptidosQuímica FísicaPlasmodium falciparumEnlace proteicoEstructura de la proteínaAnimalChemistryHaplorhinConformación de proteínasBiología574600SyntheticVaccinesProlineSynthetic PeptideSodium ChlorideRecombinant VaccineAnimal ModelAnimal ExperimentAntigen Antibody ReactionAotusBinding SiteControlled StudyDrug StructureElectronHydrogen BondImmune Protection Inducing ProteinStructureImmune ResponseImmunoassayBinding ProteinEpitopeHla Dr AntigenHydrogenMalaria VaccineOxygenImmunogenicityNonhumanParasite VirulencePeptide SynthesisPhysical ChemistryPlasmodium FalciparumProtein BindingProtein StructureChemistryProtein ConformationHaplorhiniAnimalsElectronsNitrogenInmunologíaProteínas portadorasAntígenosIMPIPS : The Immune Protection-Inducing Protein Structure concept in the search for steric-electron and topochemical principles for complete fully-protective chemically synthesised vaccine developmentarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Patarroyo, Manuel ElkinBermudez, AdrianaAlba, Martha PatriciaVanegas, MagnoliaMoreno-Vranich, ArmandoPoloche, Luis AntonioPatarroyo, Manuel A.Patarroyo, Manuel ElkinBermudez, AdrianaAlba, Martha PatriciaVanegas, MagnoliaMoreno-Vranich, ArmandoPoloche, Luis AntonioPatarroyo, Manuel AlfonsoORIGINAL85.pdfapplication/pdf8109969https://repository.urosario.edu.co/bitstreams/5c3e4b9d-c51c-45c0-ab7e-ab08947f093e/downloada629897ae0795a1ade2d9bd4575348c7MD51TEXT85.pdf.txt85.pdf.txtExtracted texttext/plain105098https://repository.urosario.edu.co/bitstreams/cddf8af5-7e9a-473d-af4a-d3501a318e9d/download2eecaaeca1331fe3a896e524b22d58efMD52THUMBNAIL85.pdf.jpg85.pdf.jpgGenerated Thumbnailimage/jpeg4309https://repository.urosario.edu.co/bitstreams/58b1f5bc-f1c8-48e0-9474-355a488df457/download178ebe141e7cb07c3cf8b81b73f19cffMD5310336/19030oai:repository.urosario.edu.co:10336/190302022-08-25 16:34:30.841https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co