The effect of Lucilia sericata- and Sarconesiopsis magellanica-derived larval therapy on Leishmania panamensis
This study's main objective was to evaluate the action of larval therapy derived from Lucilia sericata and Sarconesiopsis magellanica (blowflies) regarding Leishmania panamensis using an in vivo model. Eighteen golden hamsters (Mesocricetus auratus) were used; they were divided into 6 groups. T...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2016
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/23663
- Acceso en línea:
- https://doi.org/10.1016/j.actatropica.2016.09.020
https://repository.urosario.edu.co/handle/10336/23663
- Palabra clave:
- Experimental study
Fly
Histopathology
Larva
Parasite
Rodent
Animal experiment
Animal model
Animal tissue
Antibacterial activity
Article
Calliphoridae
Clinical effectiveness
Controlled study
Disease severity
Escherichia coli infection
In vivo study
Klebsiella pneumoniae infection
Leishmania panamensis
Lucilia sericata
Maggot therapy
Mixed infection
Nonhuman
Sarconesiopsis magellanica
Scar formation
Skin leishmaniasis
Skin ulcer
Syrian hamster
Animal
Biological therapy
Debridement
Diptera
Human
Larva
Leishmania guyanensis
Mesocricetus
Mixed infection
Parasitology
Procedures
Secretion (process)
Treatment outcome
Ulcer
Animalia
Bacteria (microorganisms)
Calliphoridae
Cricetinae
Leishmania panamensis
Lucilia sericata
Mesocricetus auratus
Antiinfective agent
Insect protein
Animals
Anti-bacterial agents
Biological therapy
Coinfection
Debridement
Diptera
Humans
Insect proteins
Larva
Leishmania guyanensis
Mesocricetus
Treatment outcome
Ulcer
Co-infection
Hamster
Larval excretions and secretions
Larval therapy
Leishmania panamensis
Lucilia sericata
Sarconesiopsis magellanica
cutaneous
cutaneous
Leishmaniasis
Leishmaniasis
- Rights
- License
- Abierto (Texto Completo)
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3a39d4a1-8a8d-431d-951a-63c54f374456-1be5e3bfd-0002-4551-8df5-71f3e76905dc-11ae314a6-f168-4186-a182-160d2a3559e2-1abb2d15e-6cdc-4efd-aab5-1e89ef146464-102cc4282-7e7f-4921-a50c-2e17247ee6a8-1bcda3ce4-3ed7-466e-beed-dda61974c622-179653065-1743756f9-9669-44b0-8a52-23524ddfcda2-12020-05-26T00:04:09Z2020-05-26T00:04:09Z2016This study's main objective was to evaluate the action of larval therapy derived from Lucilia sericata and Sarconesiopsis magellanica (blowflies) regarding Leishmania panamensis using an in vivo model. Eighteen golden hamsters (Mesocricetus auratus) were used; they were divided into 6 groups. The first three groups consisted of 4 animals each; these, in turn, were internally distributed into subgroups consisting of 2 hamsters to be used separately in treatments derived from each blowfly species. Group 1 was used in treating leishmanial lesions with larval therapy (LT), whilst the other two groups were used for evaluating the used of larval excretions and secretions (ES) after the ulcers had formed (group 2) and before they appeared (group 3). The three remaining groups (4, 5 and 6), consisting of two animals, were used as controls in the experiments. Biopsies were taken for histopathological and molecular analysis before, during and after the treatments; biopsies and smears were taken for assessing parasite presence and bacterial co-infection. LT and larval ES proved effective in treating the ulcers caused by the parasite. There were no statistically significant differences between the blowfly species regarding the ulcer cicatrisation parameters. There were granulomas in samples taken from lesions at the end of the treatments. The antibacterial action of larval treatment regarding co-infection in lesions caused by the parasite was also verified. These results potentially validate effective LT treatment against cutaneous leishmaniasis aimed at using it with humans in the future. © 2016 Elsevier B.V.application/pdfhttps://doi.org/10.1016/j.actatropica.2016.09.0200001706Xhttps://repository.urosario.edu.co/handle/10336/23663engElsevier B.V.289280Acta TropicaVol. 164Acta Tropica, ISSN:0001706X, Vol.164,(2016); pp. 280-289https://www.scopus.com/inward/record.uri?eid=2-s2.0-84989179388&doi=10.1016%2fj.actatropica.2016.09.020&partnerID=40&md5=9226cb2a5bf9da6828288f2a78aec7a1Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURExperimental studyFlyHistopathologyLarvaParasiteRodentAnimal experimentAnimal modelAnimal tissueAntibacterial activityArticleCalliphoridaeClinical effectivenessControlled studyDisease severityEscherichia coli infectionIn vivo studyKlebsiella pneumoniae infectionLeishmania panamensisLucilia sericataMaggot therapyMixed infectionNonhumanSarconesiopsis magellanicaScar formationSkin leishmaniasisSkin ulcerSyrian hamsterAnimalBiological therapyDebridementDipteraHumanLarvaLeishmania guyanensisMesocricetusMixed infectionParasitologyProceduresSecretion (process)Treatment outcomeUlcerAnimaliaBacteria (microorganisms)CalliphoridaeCricetinaeLeishmania panamensisLucilia sericataMesocricetus auratusAntiinfective agentInsect proteinAnimalsAnti-bacterial agentsBiological therapyCoinfectionDebridementDipteraHumansInsect proteinsLarvaLeishmania guyanensisMesocricetusTreatment outcomeUlcerCo-infectionHamsterLarval excretions and secretionsLarval therapyLeishmania panamensisLucilia sericataSarconesiopsis magellanicacutaneouscutaneousLeishmaniasisLeishmaniasisThe effect of Lucilia sericata- and Sarconesiopsis magellanica-derived larval therapy on Leishmania panamensisarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Cruz-Saavedra, LissaDíaz-Roa, AndreaGaona, María A.Cruz, Mónica L.Ayala, MarthaCortés-Vecino, Jesús A.Patarroyo, Manuel A.Bello, Felio J.10336/23663oai:repository.urosario.edu.co:10336/236632022-05-02 07:37:14.633531https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |
dc.title.spa.fl_str_mv |
The effect of Lucilia sericata- and Sarconesiopsis magellanica-derived larval therapy on Leishmania panamensis |
title |
The effect of Lucilia sericata- and Sarconesiopsis magellanica-derived larval therapy on Leishmania panamensis |
spellingShingle |
The effect of Lucilia sericata- and Sarconesiopsis magellanica-derived larval therapy on Leishmania panamensis Experimental study Fly Histopathology Larva Parasite Rodent Animal experiment Animal model Animal tissue Antibacterial activity Article Calliphoridae Clinical effectiveness Controlled study Disease severity Escherichia coli infection In vivo study Klebsiella pneumoniae infection Leishmania panamensis Lucilia sericata Maggot therapy Mixed infection Nonhuman Sarconesiopsis magellanica Scar formation Skin leishmaniasis Skin ulcer Syrian hamster Animal Biological therapy Debridement Diptera Human Larva Leishmania guyanensis Mesocricetus Mixed infection Parasitology Procedures Secretion (process) Treatment outcome Ulcer Animalia Bacteria (microorganisms) Calliphoridae Cricetinae Leishmania panamensis Lucilia sericata Mesocricetus auratus Antiinfective agent Insect protein Animals Anti-bacterial agents Biological therapy Coinfection Debridement Diptera Humans Insect proteins Larva Leishmania guyanensis Mesocricetus Treatment outcome Ulcer Co-infection Hamster Larval excretions and secretions Larval therapy Leishmania panamensis Lucilia sericata Sarconesiopsis magellanica cutaneous cutaneous Leishmaniasis Leishmaniasis |
title_short |
The effect of Lucilia sericata- and Sarconesiopsis magellanica-derived larval therapy on Leishmania panamensis |
title_full |
The effect of Lucilia sericata- and Sarconesiopsis magellanica-derived larval therapy on Leishmania panamensis |
title_fullStr |
The effect of Lucilia sericata- and Sarconesiopsis magellanica-derived larval therapy on Leishmania panamensis |
title_full_unstemmed |
The effect of Lucilia sericata- and Sarconesiopsis magellanica-derived larval therapy on Leishmania panamensis |
title_sort |
The effect of Lucilia sericata- and Sarconesiopsis magellanica-derived larval therapy on Leishmania panamensis |
dc.subject.keyword.spa.fl_str_mv |
Experimental study Fly Histopathology Larva Parasite Rodent Animal experiment Animal model Animal tissue Antibacterial activity Article Calliphoridae Clinical effectiveness Controlled study Disease severity Escherichia coli infection In vivo study Klebsiella pneumoniae infection Leishmania panamensis Lucilia sericata Maggot therapy Mixed infection Nonhuman Sarconesiopsis magellanica Scar formation Skin leishmaniasis Skin ulcer Syrian hamster Animal Biological therapy Debridement Diptera Human Larva Leishmania guyanensis Mesocricetus Mixed infection Parasitology Procedures Secretion (process) Treatment outcome Ulcer Animalia Bacteria (microorganisms) Calliphoridae Cricetinae Leishmania panamensis Lucilia sericata Mesocricetus auratus Antiinfective agent Insect protein Animals Anti-bacterial agents Biological therapy Coinfection Debridement Diptera Humans Insect proteins Larva Leishmania guyanensis Mesocricetus Treatment outcome Ulcer Co-infection Hamster Larval excretions and secretions Larval therapy Leishmania panamensis Lucilia sericata Sarconesiopsis magellanica |
topic |
Experimental study Fly Histopathology Larva Parasite Rodent Animal experiment Animal model Animal tissue Antibacterial activity Article Calliphoridae Clinical effectiveness Controlled study Disease severity Escherichia coli infection In vivo study Klebsiella pneumoniae infection Leishmania panamensis Lucilia sericata Maggot therapy Mixed infection Nonhuman Sarconesiopsis magellanica Scar formation Skin leishmaniasis Skin ulcer Syrian hamster Animal Biological therapy Debridement Diptera Human Larva Leishmania guyanensis Mesocricetus Mixed infection Parasitology Procedures Secretion (process) Treatment outcome Ulcer Animalia Bacteria (microorganisms) Calliphoridae Cricetinae Leishmania panamensis Lucilia sericata Mesocricetus auratus Antiinfective agent Insect protein Animals Anti-bacterial agents Biological therapy Coinfection Debridement Diptera Humans Insect proteins Larva Leishmania guyanensis Mesocricetus Treatment outcome Ulcer Co-infection Hamster Larval excretions and secretions Larval therapy Leishmania panamensis Lucilia sericata Sarconesiopsis magellanica cutaneous cutaneous Leishmaniasis Leishmaniasis |
dc.subject.keyword.eng.fl_str_mv |
cutaneous cutaneous Leishmaniasis Leishmaniasis |
description |
This study's main objective was to evaluate the action of larval therapy derived from Lucilia sericata and Sarconesiopsis magellanica (blowflies) regarding Leishmania panamensis using an in vivo model. Eighteen golden hamsters (Mesocricetus auratus) were used; they were divided into 6 groups. The first three groups consisted of 4 animals each; these, in turn, were internally distributed into subgroups consisting of 2 hamsters to be used separately in treatments derived from each blowfly species. Group 1 was used in treating leishmanial lesions with larval therapy (LT), whilst the other two groups were used for evaluating the used of larval excretions and secretions (ES) after the ulcers had formed (group 2) and before they appeared (group 3). The three remaining groups (4, 5 and 6), consisting of two animals, were used as controls in the experiments. Biopsies were taken for histopathological and molecular analysis before, during and after the treatments; biopsies and smears were taken for assessing parasite presence and bacterial co-infection. LT and larval ES proved effective in treating the ulcers caused by the parasite. There were no statistically significant differences between the blowfly species regarding the ulcer cicatrisation parameters. There were granulomas in samples taken from lesions at the end of the treatments. The antibacterial action of larval treatment regarding co-infection in lesions caused by the parasite was also verified. These results potentially validate effective LT treatment against cutaneous leishmaniasis aimed at using it with humans in the future. © 2016 Elsevier B.V. |
publishDate |
2016 |
dc.date.created.spa.fl_str_mv |
2016 |
dc.date.accessioned.none.fl_str_mv |
2020-05-26T00:04:09Z |
dc.date.available.none.fl_str_mv |
2020-05-26T00:04:09Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1016/j.actatropica.2016.09.020 |
dc.identifier.issn.none.fl_str_mv |
0001706X |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/23663 |
url |
https://doi.org/10.1016/j.actatropica.2016.09.020 https://repository.urosario.edu.co/handle/10336/23663 |
identifier_str_mv |
0001706X |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.citationEndPage.none.fl_str_mv |
289 |
dc.relation.citationStartPage.none.fl_str_mv |
280 |
dc.relation.citationTitle.none.fl_str_mv |
Acta Tropica |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 164 |
dc.relation.ispartof.spa.fl_str_mv |
Acta Tropica, ISSN:0001706X, Vol.164,(2016); pp. 280-289 |
dc.relation.uri.spa.fl_str_mv |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84989179388&doi=10.1016%2fj.actatropica.2016.09.020&partnerID=40&md5=9226cb2a5bf9da6828288f2a78aec7a1 |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.acceso.spa.fl_str_mv |
Abierto (Texto Completo) |
rights_invalid_str_mv |
Abierto (Texto Completo) http://purl.org/coar/access_right/c_abf2 |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Elsevier B.V. |
institution |
Universidad del Rosario |
dc.source.instname.spa.fl_str_mv |
instname:Universidad del Rosario |
dc.source.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional EdocUR |
repository.name.fl_str_mv |
Repositorio institucional EdocUR |
repository.mail.fl_str_mv |
edocur@urosario.edu.co |
_version_ |
1814167709275914240 |