The effect of Lucilia sericata- and Sarconesiopsis magellanica-derived larval therapy on Leishmania panamensis

This study's main objective was to evaluate the action of larval therapy derived from Lucilia sericata and Sarconesiopsis magellanica (blowflies) regarding Leishmania panamensis using an in vivo model. Eighteen golden hamsters (Mesocricetus auratus) were used; they were divided into 6 groups. T...

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Autores:
Tipo de recurso:
Fecha de publicación:
2016
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/23663
Acceso en línea:
https://doi.org/10.1016/j.actatropica.2016.09.020
https://repository.urosario.edu.co/handle/10336/23663
Palabra clave:
Experimental study
Fly
Histopathology
Larva
Parasite
Rodent
Animal experiment
Animal model
Animal tissue
Antibacterial activity
Article
Calliphoridae
Clinical effectiveness
Controlled study
Disease severity
Escherichia coli infection
In vivo study
Klebsiella pneumoniae infection
Leishmania panamensis
Lucilia sericata
Maggot therapy
Mixed infection
Nonhuman
Sarconesiopsis magellanica
Scar formation
Skin leishmaniasis
Skin ulcer
Syrian hamster
Animal
Biological therapy
Debridement
Diptera
Human
Larva
Leishmania guyanensis
Mesocricetus
Mixed infection
Parasitology
Procedures
Secretion (process)
Treatment outcome
Ulcer
Animalia
Bacteria (microorganisms)
Calliphoridae
Cricetinae
Leishmania panamensis
Lucilia sericata
Mesocricetus auratus
Antiinfective agent
Insect protein
Animals
Anti-bacterial agents
Biological therapy
Coinfection
Debridement
Diptera
Humans
Insect proteins
Larva
Leishmania guyanensis
Mesocricetus
Treatment outcome
Ulcer
Co-infection
Hamster
Larval excretions and secretions
Larval therapy
Leishmania panamensis
Lucilia sericata
Sarconesiopsis magellanica
cutaneous
cutaneous
Leishmaniasis
Leishmaniasis
Rights
License
Abierto (Texto Completo)
id EDOCUR2_7021d9106ff444e272814f84359df4e8
oai_identifier_str oai:repository.urosario.edu.co:10336/23663
network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
spelling 3a39d4a1-8a8d-431d-951a-63c54f374456-1be5e3bfd-0002-4551-8df5-71f3e76905dc-11ae314a6-f168-4186-a182-160d2a3559e2-1abb2d15e-6cdc-4efd-aab5-1e89ef146464-102cc4282-7e7f-4921-a50c-2e17247ee6a8-1bcda3ce4-3ed7-466e-beed-dda61974c622-179653065-1743756f9-9669-44b0-8a52-23524ddfcda2-12020-05-26T00:04:09Z2020-05-26T00:04:09Z2016This study's main objective was to evaluate the action of larval therapy derived from Lucilia sericata and Sarconesiopsis magellanica (blowflies) regarding Leishmania panamensis using an in vivo model. Eighteen golden hamsters (Mesocricetus auratus) were used; they were divided into 6 groups. The first three groups consisted of 4 animals each; these, in turn, were internally distributed into subgroups consisting of 2 hamsters to be used separately in treatments derived from each blowfly species. Group 1 was used in treating leishmanial lesions with larval therapy (LT), whilst the other two groups were used for evaluating the used of larval excretions and secretions (ES) after the ulcers had formed (group 2) and before they appeared (group 3). The three remaining groups (4, 5 and 6), consisting of two animals, were used as controls in the experiments. Biopsies were taken for histopathological and molecular analysis before, during and after the treatments; biopsies and smears were taken for assessing parasite presence and bacterial co-infection. LT and larval ES proved effective in treating the ulcers caused by the parasite. There were no statistically significant differences between the blowfly species regarding the ulcer cicatrisation parameters. There were granulomas in samples taken from lesions at the end of the treatments. The antibacterial action of larval treatment regarding co-infection in lesions caused by the parasite was also verified. These results potentially validate effective LT treatment against cutaneous leishmaniasis aimed at using it with humans in the future. © 2016 Elsevier B.V.application/pdfhttps://doi.org/10.1016/j.actatropica.2016.09.0200001706Xhttps://repository.urosario.edu.co/handle/10336/23663engElsevier B.V.289280Acta TropicaVol. 164Acta Tropica, ISSN:0001706X, Vol.164,(2016); pp. 280-289https://www.scopus.com/inward/record.uri?eid=2-s2.0-84989179388&doi=10.1016%2fj.actatropica.2016.09.020&partnerID=40&md5=9226cb2a5bf9da6828288f2a78aec7a1Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURExperimental studyFlyHistopathologyLarvaParasiteRodentAnimal experimentAnimal modelAnimal tissueAntibacterial activityArticleCalliphoridaeClinical effectivenessControlled studyDisease severityEscherichia coli infectionIn vivo studyKlebsiella pneumoniae infectionLeishmania panamensisLucilia sericataMaggot therapyMixed infectionNonhumanSarconesiopsis magellanicaScar formationSkin leishmaniasisSkin ulcerSyrian hamsterAnimalBiological therapyDebridementDipteraHumanLarvaLeishmania guyanensisMesocricetusMixed infectionParasitologyProceduresSecretion (process)Treatment outcomeUlcerAnimaliaBacteria (microorganisms)CalliphoridaeCricetinaeLeishmania panamensisLucilia sericataMesocricetus auratusAntiinfective agentInsect proteinAnimalsAnti-bacterial agentsBiological therapyCoinfectionDebridementDipteraHumansInsect proteinsLarvaLeishmania guyanensisMesocricetusTreatment outcomeUlcerCo-infectionHamsterLarval excretions and secretionsLarval therapyLeishmania panamensisLucilia sericataSarconesiopsis magellanicacutaneouscutaneousLeishmaniasisLeishmaniasisThe effect of Lucilia sericata- and Sarconesiopsis magellanica-derived larval therapy on Leishmania panamensisarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Cruz-Saavedra, LissaDíaz-Roa, AndreaGaona, María A.Cruz, Mónica L.Ayala, MarthaCortés-Vecino, Jesús A.Patarroyo, Manuel A.Bello, Felio J.10336/23663oai:repository.urosario.edu.co:10336/236632022-05-02 07:37:14.633531https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv The effect of Lucilia sericata- and Sarconesiopsis magellanica-derived larval therapy on Leishmania panamensis
title The effect of Lucilia sericata- and Sarconesiopsis magellanica-derived larval therapy on Leishmania panamensis
spellingShingle The effect of Lucilia sericata- and Sarconesiopsis magellanica-derived larval therapy on Leishmania panamensis
Experimental study
Fly
Histopathology
Larva
Parasite
Rodent
Animal experiment
Animal model
Animal tissue
Antibacterial activity
Article
Calliphoridae
Clinical effectiveness
Controlled study
Disease severity
Escherichia coli infection
In vivo study
Klebsiella pneumoniae infection
Leishmania panamensis
Lucilia sericata
Maggot therapy
Mixed infection
Nonhuman
Sarconesiopsis magellanica
Scar formation
Skin leishmaniasis
Skin ulcer
Syrian hamster
Animal
Biological therapy
Debridement
Diptera
Human
Larva
Leishmania guyanensis
Mesocricetus
Mixed infection
Parasitology
Procedures
Secretion (process)
Treatment outcome
Ulcer
Animalia
Bacteria (microorganisms)
Calliphoridae
Cricetinae
Leishmania panamensis
Lucilia sericata
Mesocricetus auratus
Antiinfective agent
Insect protein
Animals
Anti-bacterial agents
Biological therapy
Coinfection
Debridement
Diptera
Humans
Insect proteins
Larva
Leishmania guyanensis
Mesocricetus
Treatment outcome
Ulcer
Co-infection
Hamster
Larval excretions and secretions
Larval therapy
Leishmania panamensis
Lucilia sericata
Sarconesiopsis magellanica
cutaneous
cutaneous
Leishmaniasis
Leishmaniasis
title_short The effect of Lucilia sericata- and Sarconesiopsis magellanica-derived larval therapy on Leishmania panamensis
title_full The effect of Lucilia sericata- and Sarconesiopsis magellanica-derived larval therapy on Leishmania panamensis
title_fullStr The effect of Lucilia sericata- and Sarconesiopsis magellanica-derived larval therapy on Leishmania panamensis
title_full_unstemmed The effect of Lucilia sericata- and Sarconesiopsis magellanica-derived larval therapy on Leishmania panamensis
title_sort The effect of Lucilia sericata- and Sarconesiopsis magellanica-derived larval therapy on Leishmania panamensis
dc.subject.keyword.spa.fl_str_mv Experimental study
Fly
Histopathology
Larva
Parasite
Rodent
Animal experiment
Animal model
Animal tissue
Antibacterial activity
Article
Calliphoridae
Clinical effectiveness
Controlled study
Disease severity
Escherichia coli infection
In vivo study
Klebsiella pneumoniae infection
Leishmania panamensis
Lucilia sericata
Maggot therapy
Mixed infection
Nonhuman
Sarconesiopsis magellanica
Scar formation
Skin leishmaniasis
Skin ulcer
Syrian hamster
Animal
Biological therapy
Debridement
Diptera
Human
Larva
Leishmania guyanensis
Mesocricetus
Mixed infection
Parasitology
Procedures
Secretion (process)
Treatment outcome
Ulcer
Animalia
Bacteria (microorganisms)
Calliphoridae
Cricetinae
Leishmania panamensis
Lucilia sericata
Mesocricetus auratus
Antiinfective agent
Insect protein
Animals
Anti-bacterial agents
Biological therapy
Coinfection
Debridement
Diptera
Humans
Insect proteins
Larva
Leishmania guyanensis
Mesocricetus
Treatment outcome
Ulcer
Co-infection
Hamster
Larval excretions and secretions
Larval therapy
Leishmania panamensis
Lucilia sericata
Sarconesiopsis magellanica
topic Experimental study
Fly
Histopathology
Larva
Parasite
Rodent
Animal experiment
Animal model
Animal tissue
Antibacterial activity
Article
Calliphoridae
Clinical effectiveness
Controlled study
Disease severity
Escherichia coli infection
In vivo study
Klebsiella pneumoniae infection
Leishmania panamensis
Lucilia sericata
Maggot therapy
Mixed infection
Nonhuman
Sarconesiopsis magellanica
Scar formation
Skin leishmaniasis
Skin ulcer
Syrian hamster
Animal
Biological therapy
Debridement
Diptera
Human
Larva
Leishmania guyanensis
Mesocricetus
Mixed infection
Parasitology
Procedures
Secretion (process)
Treatment outcome
Ulcer
Animalia
Bacteria (microorganisms)
Calliphoridae
Cricetinae
Leishmania panamensis
Lucilia sericata
Mesocricetus auratus
Antiinfective agent
Insect protein
Animals
Anti-bacterial agents
Biological therapy
Coinfection
Debridement
Diptera
Humans
Insect proteins
Larva
Leishmania guyanensis
Mesocricetus
Treatment outcome
Ulcer
Co-infection
Hamster
Larval excretions and secretions
Larval therapy
Leishmania panamensis
Lucilia sericata
Sarconesiopsis magellanica
cutaneous
cutaneous
Leishmaniasis
Leishmaniasis
dc.subject.keyword.eng.fl_str_mv cutaneous
cutaneous
Leishmaniasis
Leishmaniasis
description This study's main objective was to evaluate the action of larval therapy derived from Lucilia sericata and Sarconesiopsis magellanica (blowflies) regarding Leishmania panamensis using an in vivo model. Eighteen golden hamsters (Mesocricetus auratus) were used; they were divided into 6 groups. The first three groups consisted of 4 animals each; these, in turn, were internally distributed into subgroups consisting of 2 hamsters to be used separately in treatments derived from each blowfly species. Group 1 was used in treating leishmanial lesions with larval therapy (LT), whilst the other two groups were used for evaluating the used of larval excretions and secretions (ES) after the ulcers had formed (group 2) and before they appeared (group 3). The three remaining groups (4, 5 and 6), consisting of two animals, were used as controls in the experiments. Biopsies were taken for histopathological and molecular analysis before, during and after the treatments; biopsies and smears were taken for assessing parasite presence and bacterial co-infection. LT and larval ES proved effective in treating the ulcers caused by the parasite. There were no statistically significant differences between the blowfly species regarding the ulcer cicatrisation parameters. There were granulomas in samples taken from lesions at the end of the treatments. The antibacterial action of larval treatment regarding co-infection in lesions caused by the parasite was also verified. These results potentially validate effective LT treatment against cutaneous leishmaniasis aimed at using it with humans in the future. © 2016 Elsevier B.V.
publishDate 2016
dc.date.created.spa.fl_str_mv 2016
dc.date.accessioned.none.fl_str_mv 2020-05-26T00:04:09Z
dc.date.available.none.fl_str_mv 2020-05-26T00:04:09Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1016/j.actatropica.2016.09.020
dc.identifier.issn.none.fl_str_mv 0001706X
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/23663
url https://doi.org/10.1016/j.actatropica.2016.09.020
https://repository.urosario.edu.co/handle/10336/23663
identifier_str_mv 0001706X
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 289
dc.relation.citationStartPage.none.fl_str_mv 280
dc.relation.citationTitle.none.fl_str_mv Acta Tropica
dc.relation.citationVolume.none.fl_str_mv Vol. 164
dc.relation.ispartof.spa.fl_str_mv Acta Tropica, ISSN:0001706X, Vol.164,(2016); pp. 280-289
dc.relation.uri.spa.fl_str_mv https://www.scopus.com/inward/record.uri?eid=2-s2.0-84989179388&doi=10.1016%2fj.actatropica.2016.09.020&partnerID=40&md5=9226cb2a5bf9da6828288f2a78aec7a1
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
rights_invalid_str_mv Abierto (Texto Completo)
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Elsevier B.V.
institution Universidad del Rosario
dc.source.instname.spa.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.spa.fl_str_mv reponame:Repositorio Institucional EdocUR
repository.name.fl_str_mv Repositorio institucional EdocUR
repository.mail.fl_str_mv edocur@urosario.edu.co
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