Actividad de beta-galactosidasa como marcador de senescencia en cultivos primarios del epitelio de la superficie ovárica

beta-galaetosidase activity reflects the rate of cellular aging in vitro. Such activity was quantified at pH 6 in ovarian epithelial cells from 28 donors without a history of cancer, by the chemoluminiscent method. The cells were serially cultured until they achieved the state of permanent growth arrest. During the exponential growth phase, all cultures showed a similar pattern of growth and low beta-galitetosidase activity. However, both in the onset of decrease replicative activity, as well as in the onset of the stationary phase, there was a significant rise in the enzyme activity. Our results showed that P-galactosidase activity can be considered as a replicative senescence marker of the ovarian surface epithelium at pH 6.

• Fecha de publicación: 2010
• Institución: Universidad del Rosario
• Repositorio: Repositorio EdocUR - U. Rosario
• Idioma: spa
• OAI Identifier: oai:repository.urosario.edu.co:10336/24728
• Acceso en línea: https://repository.urosario.edu.co/handle/10336/24728
• Palabra clave: beta-galactosidasa; senescencia replicativa; biomarcador; células epiteliales de ovario; Senescencia celular; Senescencia replicativa; Células; Cáncer; Biomarcador; Supresión; Incrementar; Biología; Años; beta-galactosidase; replicative senescence; biomarker; ovarian epithelial cells; Cellular senescence; Replicative senescence; Cells; Cancer; Biomarker; Suppression; Increase; Biology; Age
• License: Bloqueado (Texto referencial)