Actividad de beta-galactosidasa como marcador de senescencia en cultivos primarios del epitelio de la superficie ovárica

beta-galaetosidase activity reflects the rate of cellular aging in vitro. Such activity was quantified at pH 6 in ovarian epithelial cells from 28 donors without a history of cancer, by the chemoluminiscent method. The cells were serially cultured until they achieved the state of permanent growth ar...

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Autores:
Tipo de recurso:
Fecha de publicación:
2010
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
spa
OAI Identifier:
oai:repository.urosario.edu.co:10336/24728
Acceso en línea:
https://repository.urosario.edu.co/handle/10336/24728
Palabra clave:
beta-galactosidasa
senescencia replicativa
biomarcador
células epiteliales de ovario
Senescencia celular
Senescencia replicativa
Células
Cáncer
Biomarcador
Supresión
Incrementar
Biología
Años
beta-galactosidase
replicative senescence
biomarker
ovarian epithelial cells
Cellular senescence
Replicative senescence
Cells
Cancer
Biomarker
Suppression
Increase
Biology
Age
Rights
License
Bloqueado (Texto referencial)
Description
Summary:beta-galaetosidase activity reflects the rate of cellular aging in vitro. Such activity was quantified at pH 6 in ovarian epithelial cells from 28 donors without a history of cancer, by the chemoluminiscent method. The cells were serially cultured until they achieved the state of permanent growth arrest. During the exponential growth phase, all cultures showed a similar pattern of growth and low beta-galitetosidase activity. However, both in the onset of decrease replicative activity, as well as in the onset of the stationary phase, there was a significant rise in the enzyme activity. Our results showed that P-galactosidase activity can be considered as a replicative senescence marker of the ovarian surface epithelium at pH 6.