A DNA barcode for land plants

DNA barcoding involves sequencing a standard region of DNA as a tool for species identification. However, there has been no agreement on which region(s) should be used for barcoding land plants. To provide a community recommendation on a standard plant barcode, we have compared the performance of 7...

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Tipo de recurso:
Fecha de publicación:
2009
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/27409
Acceso en línea:
https://doi.org/10.1073/pnas.0905845106
https://repository.urosario.edu.co/handle/10336/27409
Palabra clave:
Mitochondrial
Barcoding
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License
Abierto (Texto Completo)
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spelling 3593286001b96e5c9-e518-4077-98b7-e7b73fa08d538a2577ee-ed01-43b9-827e-08dbd5004a97ce234a75-a3bf-47dd-9727-8bdd038a18272020-08-19T14:42:05Z2020-08-19T14:42:05Z2009-05-27DNA barcoding involves sequencing a standard region of DNA as a tool for species identification. However, there has been no agreement on which region(s) should be used for barcoding land plants. To provide a community recommendation on a standard plant barcode, we have compared the performance of 7 leading candidate plastid DNA regions (atpF–atpH spacer, matK gene, rbcL gene, rpoB gene, rpoC1 gene, psbK–psbI spacer, and trnH–psbA spacer). Based on assessments of recoverability, sequence quality, and levels of species discrimination, we recommend the 2-locus combination of rbcL matK as the plant barcode. This core 2-locus barcode will provide a universal framework for the routine use of DNA sequence data to identify specimens and contribute toward the discovery of overlooked species of land plants.application/pdfhttps://doi.org/10.1073/pnas.0905845106ISSN: 0027-8424EISSN: 1091-6490https://repository.urosario.edu.co/handle/10336/27409engProceedings of the National Academy of Sciences of the United States of America12797No. 3112794Proceedings of the National Academy of Sciences of the United States of AmericaVol. 106Proceedings of the National Academy of Sciences of the United States of America, ISSN: 0027-8424; EISSN: 1091-6490, Vol.106, No.31 (2009); pp. 12794 –12797https://www.pnas.org/content/pnas/106/31/12794.full.pdfAbierto (Texto Completo)http://purl.org/coar/access_right/c_abf2Proceedings of the National Academy of Sciences of the United States of Americainstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURMitochondrialBarcodingA DNA barcode for land plantsUn código de barras de ADN para plantas terrestresarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Richardson, James-EdwardErickson, David L.Burgess, KevinLong, David G.10336/27409oai:repository.urosario.edu.co:10336/274092021-10-07 23:33:58.852https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv A DNA barcode for land plants
dc.title.TranslatedTitle.spa.fl_str_mv Un código de barras de ADN para plantas terrestres
title A DNA barcode for land plants
spellingShingle A DNA barcode for land plants
Mitochondrial
Barcoding
title_short A DNA barcode for land plants
title_full A DNA barcode for land plants
title_fullStr A DNA barcode for land plants
title_full_unstemmed A DNA barcode for land plants
title_sort A DNA barcode for land plants
dc.subject.keyword.spa.fl_str_mv Mitochondrial
Barcoding
topic Mitochondrial
Barcoding
description DNA barcoding involves sequencing a standard region of DNA as a tool for species identification. However, there has been no agreement on which region(s) should be used for barcoding land plants. To provide a community recommendation on a standard plant barcode, we have compared the performance of 7 leading candidate plastid DNA regions (atpF–atpH spacer, matK gene, rbcL gene, rpoB gene, rpoC1 gene, psbK–psbI spacer, and trnH–psbA spacer). Based on assessments of recoverability, sequence quality, and levels of species discrimination, we recommend the 2-locus combination of rbcL matK as the plant barcode. This core 2-locus barcode will provide a universal framework for the routine use of DNA sequence data to identify specimens and contribute toward the discovery of overlooked species of land plants.
publishDate 2009
dc.date.created.spa.fl_str_mv 2009-05-27
dc.date.accessioned.none.fl_str_mv 2020-08-19T14:42:05Z
dc.date.available.none.fl_str_mv 2020-08-19T14:42:05Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1073/pnas.0905845106
dc.identifier.issn.none.fl_str_mv ISSN: 0027-8424
EISSN: 1091-6490
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/27409
url https://doi.org/10.1073/pnas.0905845106
https://repository.urosario.edu.co/handle/10336/27409
identifier_str_mv ISSN: 0027-8424
EISSN: 1091-6490
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 12797
dc.relation.citationIssue.none.fl_str_mv No. 31
dc.relation.citationStartPage.none.fl_str_mv 12794
dc.relation.citationTitle.none.fl_str_mv Proceedings of the National Academy of Sciences of the United States of America
dc.relation.citationVolume.none.fl_str_mv Vol. 106
dc.relation.ispartof.spa.fl_str_mv Proceedings of the National Academy of Sciences of the United States of America, ISSN: 0027-8424; EISSN: 1091-6490, Vol.106, No.31 (2009); pp. 12794 –12797
dc.relation.uri.spa.fl_str_mv https://www.pnas.org/content/pnas/106/31/12794.full.pdf
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
rights_invalid_str_mv Abierto (Texto Completo)
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Proceedings of the National Academy of Sciences of the United States of America
dc.source.spa.fl_str_mv Proceedings of the National Academy of Sciences of the United States of America
institution Universidad del Rosario
dc.source.instname.none.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.none.fl_str_mv reponame:Repositorio Institucional EdocUR
repository.name.fl_str_mv Repositorio institucional EdocUR
repository.mail.fl_str_mv edocur@urosario.edu.co
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