The DNA load of six high-risk human papillomavirus types and its association with cervical lesions

Background: Analysing human papillomavirus (HPV) viral load is important in determining the risk of developing cervical cancer (CC); most knowledge to date regarding HPV viral load and cervical lesions has been related to HPV-16. This study evaluated the association between the viral load of the six...

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Tipo de recurso:
Fecha de publicación:
2015
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/21459
Acceso en línea:
https://doi.org/10.1186/s12885-015-1126-z
https://repository.urosario.edu.co/handle/10336/21459
Palabra clave:
HR-HPV
HPV DNA load
RT-PCR
Enfermedades
Cervical intraepithelial neoplasia
Neoplasias
Colposcopía
Rights
License
Abierto (Texto Completo)
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oai_identifier_str oai:repository.urosario.edu.co:10336/21459
network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
dc.title.spa.fl_str_mv The DNA load of six high-risk human papillomavirus types and its association with cervical lesions
title The DNA load of six high-risk human papillomavirus types and its association with cervical lesions
spellingShingle The DNA load of six high-risk human papillomavirus types and its association with cervical lesions
HR-HPV
HPV DNA load
RT-PCR
Enfermedades
Cervical intraepithelial neoplasia
Neoplasias
Colposcopía
title_short The DNA load of six high-risk human papillomavirus types and its association with cervical lesions
title_full The DNA load of six high-risk human papillomavirus types and its association with cervical lesions
title_fullStr The DNA load of six high-risk human papillomavirus types and its association with cervical lesions
title_full_unstemmed The DNA load of six high-risk human papillomavirus types and its association with cervical lesions
title_sort The DNA load of six high-risk human papillomavirus types and its association with cervical lesions
dc.subject.spa.fl_str_mv HR-HPV
HPV DNA load
RT-PCR
topic HR-HPV
HPV DNA load
RT-PCR
Enfermedades
Cervical intraepithelial neoplasia
Neoplasias
Colposcopía
dc.subject.ddc.spa.fl_str_mv Enfermedades
dc.subject.keyword.spa.fl_str_mv Cervical intraepithelial neoplasia
dc.subject.lemb.spa.fl_str_mv Neoplasias
Colposcopía
description Background: Analysing human papillomavirus (HPV) viral load is important in determining the risk of developing cervical cancer (CC); most knowledge to date regarding HPV viral load and cervical lesions has been related to HPV-16. This study evaluated the association between the viral load of the six most prevalent high-risk viral types in Colombia and cervical intraepithelial neoplasia (CIN) frequency. Methods: 114 women without CIN and 59 women having CIN confirmed by colposcopy, all of them positive by conventional PCR for HPV infection in the initial screening, were included in the study. Samples were tested for six high-risk HPV types to determine viral copy number by real-time PCR. Crude and adjusted odds ratios (ORa) were estimated for evaluating the association between each viral type's DNA load and the risk of cervical lesions occurring. Results: The highest viral loads were identified for HPV-33 in CIN patients and for HPV-31 in patients without lesions (9.33 HPV copies, 2.95 interquartile range (IQR); 9.41 HPV copies, 2.58 IQR). Lesions were more frequent in HPV-16 patients having a low viral load (3.53 ORa, 1.16-10.74 95%CI) compared to those having high HPV-16 load (2.62 ORa, 1.08-6.35 95%CI). High viral load in HPV-31 patients was associated with lower CIN frequency (0.34 ORa, 0.15-0.78 95%CI). Conclusions: An association between HPV DNA load and CIN frequency was seen to be type-specific and may have depended on the duration of infection. This analysis has provided information for understanding the effect of HPV DNA load on cervical lesion development. © 2015 applies to the data made available in this article, unless otherwise stated.
publishDate 2015
dc.date.created.none.fl_str_mv 2015
dc.date.issued.none.fl_str_mv 2015
dc.date.accessioned.none.fl_str_mv 2020-04-03T01:52:06Z
dc.date.available.none.fl_str_mv 2020-04-03T01:52:06Z
dc.type.eng.fl_str_mv article
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dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1186/s12885-015-1126-z
dc.identifier.issn.none.fl_str_mv 1471-2407
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/21459
url https://doi.org/10.1186/s12885-015-1126-z
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identifier_str_mv 1471-2407
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationIssue.none.fl_str_mv No. 1
dc.relation.citationTitle.none.fl_str_mv BMC Cancer
dc.relation.citationVolume.none.fl_str_mv Vol. 15
dc.relation.ispartof.spa.fl_str_mv BMC Cancer, ISSN: 1471-2407 Vol. 15, No. 1 (2015)
dc.relation.uri.spa.fl_str_mv https://bmccancer.biomedcentral.com/track/pdf/10.1186/s12885-015-1126-z
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rights_invalid_str_mv Abierto (Texto Completo)
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institution Universidad del Rosario
dc.source.instname.none.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.none.fl_str_mv reponame:Repositorio Institucional EdocUR
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spelling ef4c4e37-ca50-4c5e-9551-4cd91d0387856002f1edbfb-2e72-4b52-857b-dff41abaac25600c67d367e-96c2-4566-8a39-374ead1a65f6600a933d343-0788-4635-9002-4943747140c3600aa2fd24c-0eb4-4d73-8a15-c2b3025f602d600e38a19df-975a-4349-ac57-a8db8cdbadf46005f93fb9e-84e5-4c2e-8d87-5455ab50cd8c600796530656002020-04-03T01:52:06Z2020-04-03T01:52:06Z20152015Background: Analysing human papillomavirus (HPV) viral load is important in determining the risk of developing cervical cancer (CC); most knowledge to date regarding HPV viral load and cervical lesions has been related to HPV-16. This study evaluated the association between the viral load of the six most prevalent high-risk viral types in Colombia and cervical intraepithelial neoplasia (CIN) frequency. Methods: 114 women without CIN and 59 women having CIN confirmed by colposcopy, all of them positive by conventional PCR for HPV infection in the initial screening, were included in the study. Samples were tested for six high-risk HPV types to determine viral copy number by real-time PCR. Crude and adjusted odds ratios (ORa) were estimated for evaluating the association between each viral type's DNA load and the risk of cervical lesions occurring. Results: The highest viral loads were identified for HPV-33 in CIN patients and for HPV-31 in patients without lesions (9.33 HPV copies, 2.95 interquartile range (IQR); 9.41 HPV copies, 2.58 IQR). Lesions were more frequent in HPV-16 patients having a low viral load (3.53 ORa, 1.16-10.74 95%CI) compared to those having high HPV-16 load (2.62 ORa, 1.08-6.35 95%CI). High viral load in HPV-31 patients was associated with lower CIN frequency (0.34 ORa, 0.15-0.78 95%CI). Conclusions: An association between HPV DNA load and CIN frequency was seen to be type-specific and may have depended on the duration of infection. This analysis has provided information for understanding the effect of HPV DNA load on cervical lesion development. © 2015 applies to the data made available in this article, unless otherwise stated.application/pdfhttps://doi.org/10.1186/s12885-015-1126-z1471-2407https://repository.urosario.edu.co/handle/10336/21459engNo. 1BMC CancerVol. 15BMC Cancer, ISSN: 1471-2407 Vol. 15, No. 1 (2015)https://bmccancer.biomedcentral.com/track/pdf/10.1186/s12885-015-1126-zAbierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURHR-HPVHPV DNA loadRT-PCREnfermedades616600Cervical intraepithelial neoplasiaNeoplasiasColposcopíaThe DNA load of six high-risk human papillomavirus types and its association with cervical lesionsarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Río-Ospina, Luisa delSoto De León, Sara CeciliaCamargo, MilenaMoreno Pérez, Darwin AndrésSánchez, RicardoPérez Prados, AntonioPatarroyo, Manuel-ElkinPatarroyo, Manuel A.Del Río-Ospina, Luisa delSoto-De León, Sara CeciliaCamargo, MilenaMoreno-Pérez, Darwin AndrésSánchez, RicardoPérez-Prados, AntonioPatarroyo, Manuel ElkinPatarroyo, Manuel AlfonsoORIGINALThe_DNA_load_of_six_high-risk_human_papillomavirus_types_and_its_association_with_cervical_lesions.pdfapplication/pdf595764https://repository.urosario.edu.co/bitstreams/2a8f69e6-6536-47db-9019-750af12a0ca0/download50ae3d55a9bc4058fdcef9e818f9ba0fMD51TEXTThe_DNA_load_of_six_high-risk_human_papillomavirus_types_and_its_association_with_cervical_lesions.pdf.txtThe_DNA_load_of_six_high-risk_human_papillomavirus_types_and_its_association_with_cervical_lesions.pdf.txtExtracted texttext/plain52076https://repository.urosario.edu.co/bitstreams/554dcbbb-8e30-4868-98c0-1e2d974c2609/download54792b625a2a5ff2546ea394c73b69a8MD52THUMBNAILThe_DNA_load_of_six_high-risk_human_papillomavirus_types_and_its_association_with_cervical_lesions.pdf.jpgThe_DNA_load_of_six_high-risk_human_papillomavirus_types_and_its_association_with_cervical_lesions.pdf.jpgGenerated Thumbnailimage/jpeg4528https://repository.urosario.edu.co/bitstreams/efb4d8c8-08aa-4490-8072-1b0890a022f3/download7c9bdb7e34b1961e49f94fbfb0041889MD5310336/21459oai:repository.urosario.edu.co:10336/214592020-05-13 14:48:07.27https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co