The DNA load of six high-risk human papillomavirus types and its association with cervical lesions
Background: Analysing human papillomavirus (HPV) viral load is important in determining the risk of developing cervical cancer (CC); most knowledge to date regarding HPV viral load and cervical lesions has been related to HPV-16. This study evaluated the association between the viral load of the six...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2015
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/21459
- Acceso en línea:
- https://doi.org/10.1186/s12885-015-1126-z
https://repository.urosario.edu.co/handle/10336/21459
- Palabra clave:
- HR-HPV
HPV DNA load
RT-PCR
Enfermedades
Cervical intraepithelial neoplasia
Neoplasias
Colposcopía
- Rights
- License
- Abierto (Texto Completo)
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|
dc.title.spa.fl_str_mv |
The DNA load of six high-risk human papillomavirus types and its association with cervical lesions |
title |
The DNA load of six high-risk human papillomavirus types and its association with cervical lesions |
spellingShingle |
The DNA load of six high-risk human papillomavirus types and its association with cervical lesions HR-HPV HPV DNA load RT-PCR Enfermedades Cervical intraepithelial neoplasia Neoplasias Colposcopía |
title_short |
The DNA load of six high-risk human papillomavirus types and its association with cervical lesions |
title_full |
The DNA load of six high-risk human papillomavirus types and its association with cervical lesions |
title_fullStr |
The DNA load of six high-risk human papillomavirus types and its association with cervical lesions |
title_full_unstemmed |
The DNA load of six high-risk human papillomavirus types and its association with cervical lesions |
title_sort |
The DNA load of six high-risk human papillomavirus types and its association with cervical lesions |
dc.subject.spa.fl_str_mv |
HR-HPV HPV DNA load RT-PCR |
topic |
HR-HPV HPV DNA load RT-PCR Enfermedades Cervical intraepithelial neoplasia Neoplasias Colposcopía |
dc.subject.ddc.spa.fl_str_mv |
Enfermedades |
dc.subject.keyword.spa.fl_str_mv |
Cervical intraepithelial neoplasia |
dc.subject.lemb.spa.fl_str_mv |
Neoplasias Colposcopía |
description |
Background: Analysing human papillomavirus (HPV) viral load is important in determining the risk of developing cervical cancer (CC); most knowledge to date regarding HPV viral load and cervical lesions has been related to HPV-16. This study evaluated the association between the viral load of the six most prevalent high-risk viral types in Colombia and cervical intraepithelial neoplasia (CIN) frequency. Methods: 114 women without CIN and 59 women having CIN confirmed by colposcopy, all of them positive by conventional PCR for HPV infection in the initial screening, were included in the study. Samples were tested for six high-risk HPV types to determine viral copy number by real-time PCR. Crude and adjusted odds ratios (ORa) were estimated for evaluating the association between each viral type's DNA load and the risk of cervical lesions occurring. Results: The highest viral loads were identified for HPV-33 in CIN patients and for HPV-31 in patients without lesions (9.33 HPV copies, 2.95 interquartile range (IQR); 9.41 HPV copies, 2.58 IQR). Lesions were more frequent in HPV-16 patients having a low viral load (3.53 ORa, 1.16-10.74 95%CI) compared to those having high HPV-16 load (2.62 ORa, 1.08-6.35 95%CI). High viral load in HPV-31 patients was associated with lower CIN frequency (0.34 ORa, 0.15-0.78 95%CI). Conclusions: An association between HPV DNA load and CIN frequency was seen to be type-specific and may have depended on the duration of infection. This analysis has provided information for understanding the effect of HPV DNA load on cervical lesion development. © 2015 applies to the data made available in this article, unless otherwise stated. |
publishDate |
2015 |
dc.date.created.none.fl_str_mv |
2015 |
dc.date.issued.none.fl_str_mv |
2015 |
dc.date.accessioned.none.fl_str_mv |
2020-04-03T01:52:06Z |
dc.date.available.none.fl_str_mv |
2020-04-03T01:52:06Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1186/s12885-015-1126-z |
dc.identifier.issn.none.fl_str_mv |
1471-2407 |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/21459 |
url |
https://doi.org/10.1186/s12885-015-1126-z https://repository.urosario.edu.co/handle/10336/21459 |
identifier_str_mv |
1471-2407 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.citationIssue.none.fl_str_mv |
No. 1 |
dc.relation.citationTitle.none.fl_str_mv |
BMC Cancer |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 15 |
dc.relation.ispartof.spa.fl_str_mv |
BMC Cancer, ISSN: 1471-2407 Vol. 15, No. 1 (2015) |
dc.relation.uri.spa.fl_str_mv |
https://bmccancer.biomedcentral.com/track/pdf/10.1186/s12885-015-1126-z |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.acceso.spa.fl_str_mv |
Abierto (Texto Completo) |
rights_invalid_str_mv |
Abierto (Texto Completo) http://purl.org/coar/access_right/c_abf2 |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
institution |
Universidad del Rosario |
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instname:Universidad del Rosario |
dc.source.reponame.none.fl_str_mv |
reponame:Repositorio Institucional EdocUR |
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spelling |
ef4c4e37-ca50-4c5e-9551-4cd91d0387856002f1edbfb-2e72-4b52-857b-dff41abaac25600c67d367e-96c2-4566-8a39-374ead1a65f6600a933d343-0788-4635-9002-4943747140c3600aa2fd24c-0eb4-4d73-8a15-c2b3025f602d600e38a19df-975a-4349-ac57-a8db8cdbadf46005f93fb9e-84e5-4c2e-8d87-5455ab50cd8c600796530656002020-04-03T01:52:06Z2020-04-03T01:52:06Z20152015Background: Analysing human papillomavirus (HPV) viral load is important in determining the risk of developing cervical cancer (CC); most knowledge to date regarding HPV viral load and cervical lesions has been related to HPV-16. This study evaluated the association between the viral load of the six most prevalent high-risk viral types in Colombia and cervical intraepithelial neoplasia (CIN) frequency. Methods: 114 women without CIN and 59 women having CIN confirmed by colposcopy, all of them positive by conventional PCR for HPV infection in the initial screening, were included in the study. Samples were tested for six high-risk HPV types to determine viral copy number by real-time PCR. Crude and adjusted odds ratios (ORa) were estimated for evaluating the association between each viral type's DNA load and the risk of cervical lesions occurring. Results: The highest viral loads were identified for HPV-33 in CIN patients and for HPV-31 in patients without lesions (9.33 HPV copies, 2.95 interquartile range (IQR); 9.41 HPV copies, 2.58 IQR). Lesions were more frequent in HPV-16 patients having a low viral load (3.53 ORa, 1.16-10.74 95%CI) compared to those having high HPV-16 load (2.62 ORa, 1.08-6.35 95%CI). High viral load in HPV-31 patients was associated with lower CIN frequency (0.34 ORa, 0.15-0.78 95%CI). Conclusions: An association between HPV DNA load and CIN frequency was seen to be type-specific and may have depended on the duration of infection. This analysis has provided information for understanding the effect of HPV DNA load on cervical lesion development. © 2015 applies to the data made available in this article, unless otherwise stated.application/pdfhttps://doi.org/10.1186/s12885-015-1126-z1471-2407https://repository.urosario.edu.co/handle/10336/21459engNo. 1BMC CancerVol. 15BMC Cancer, ISSN: 1471-2407 Vol. 15, No. 1 (2015)https://bmccancer.biomedcentral.com/track/pdf/10.1186/s12885-015-1126-zAbierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURHR-HPVHPV DNA loadRT-PCREnfermedades616600Cervical intraepithelial neoplasiaNeoplasiasColposcopíaThe DNA load of six high-risk human papillomavirus types and its association with cervical lesionsarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Río-Ospina, Luisa delSoto De León, Sara CeciliaCamargo, MilenaMoreno Pérez, Darwin AndrésSánchez, RicardoPérez Prados, AntonioPatarroyo, Manuel-ElkinPatarroyo, Manuel A.Del Río-Ospina, Luisa delSoto-De León, Sara CeciliaCamargo, MilenaMoreno-Pérez, Darwin AndrésSánchez, RicardoPérez-Prados, AntonioPatarroyo, Manuel ElkinPatarroyo, Manuel AlfonsoORIGINALThe_DNA_load_of_six_high-risk_human_papillomavirus_types_and_its_association_with_cervical_lesions.pdfapplication/pdf595764https://repository.urosario.edu.co/bitstreams/2a8f69e6-6536-47db-9019-750af12a0ca0/download50ae3d55a9bc4058fdcef9e818f9ba0fMD51TEXTThe_DNA_load_of_six_high-risk_human_papillomavirus_types_and_its_association_with_cervical_lesions.pdf.txtThe_DNA_load_of_six_high-risk_human_papillomavirus_types_and_its_association_with_cervical_lesions.pdf.txtExtracted texttext/plain52076https://repository.urosario.edu.co/bitstreams/554dcbbb-8e30-4868-98c0-1e2d974c2609/download54792b625a2a5ff2546ea394c73b69a8MD52THUMBNAILThe_DNA_load_of_six_high-risk_human_papillomavirus_types_and_its_association_with_cervical_lesions.pdf.jpgThe_DNA_load_of_six_high-risk_human_papillomavirus_types_and_its_association_with_cervical_lesions.pdf.jpgGenerated Thumbnailimage/jpeg4528https://repository.urosario.edu.co/bitstreams/efb4d8c8-08aa-4490-8072-1b0890a022f3/download7c9bdb7e34b1961e49f94fbfb0041889MD5310336/21459oai:repository.urosario.edu.co:10336/214592020-05-13 14:48:07.27https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |