Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae)

Insect cell cultures are an important biotechnological tool for basic and applied studies. The objective of this work was to establish and characterise a new cell line from Culex quinquefasciatus embryonic tissues. Embryonated eggs were taken as a source of tissue to make explants that were seeded i...

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Tipo de recurso:
Fecha de publicación:
2012
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/22579
Acceso en línea:
https://doi.org/10.1590/S0074-02762012000100013
https://repository.urosario.edu.co/handle/10336/22579
Palabra clave:
Culex pipiens quinquefasciatus
Culicidae
Diptera
Hexapoda
Culture media
Embryonic tissues
Isoenzymatic patterns
Karyotype
RAPD-PCR
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License
Abierto (Texto Completo)
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spelling eaafab20-9002-4382-be1b-201f1742bec7-142fbddf0-7fbd-48cc-ae3d-009f774153c8-139d67619-1582-413c-a7da-7c3706659e05-1a00c7523-8e04-40b1-8983-a597d58eb894-12020-05-25T23:57:00Z2020-05-25T23:57:00Z2012Insect cell cultures are an important biotechnological tool for basic and applied studies. The objective of this work was to establish and characterise a new cell line from Culex quinquefasciatus embryonic tissues. Embryonated eggs were taken as a source of tissue to make explants that were seeded in L-15, Grace's, Grace's/L-15, MM/VP12, Schneider's and DMEM culture media with a pH range from 6.7-6.9 and incubated at 28°C. The morphological, cytogenetic, biochemical and molecular characteristics of the cell cultures were examined by observing the cell shapes, obtaining the karyotypes, using a cellulose-acetate electrophoretic system and performing random amplified polymorphic DNA-polymerase chain reaction analysis, respectively. The Grace's/L-15 medium provided the optimal nutritional conditions for cell adhesion and proliferation. Approximately 40-60 days following the explant procedure, a confluent monolayer was formed. Cellular morphology in the primary cultures and the subcultures was heterogeneous, but in the monolayer the epithelioid morphology type predominated. A karyotype with a diploid number of six chromosomes (2n = 6) was observed. Isoenzymatic and molecular patterns of the mosquito cell cultures matched those obtained from the immature and adult forms of the same species. Eighteen subcultures were generated. These cell cultures potentially constitute a useful tool for use in biomedical applications.application/pdfhttps://doi.org/10.1590/S0074-027620120001000131678806000740276https://repository.urosario.edu.co/handle/10336/22579engFundacao Oswaldo Cruz95No. 189Memorias do Instituto Oswaldo CruzVol. 107Memorias do Instituto Oswaldo Cruz, ISSN:16788060, 00740276, Vol.107, No.1 (2012); pp. 89-95https://www.scopus.com/inward/record.uri?eid=2-s2.0-84857099648&doi=10.1590%2fS0074-02762012000100013&partnerID=40&md5=95cc2f5423366c796bea1f24c5e7dfa2Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURCulex pipiens quinquefasciatusCulicidaeDipteraHexapodaCulture mediaEmbryonic tissuesIsoenzymatic patternsKaryotypeRAPD-PCREstablishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae)articleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Segura, Nidya ASantamaría, ErikaCabrera, Olga LBello, FelioORIGINAL13.pdfapplication/pdf675310https://repository.urosario.edu.co/bitstreams/103dd855-10cf-488d-bf45-622d7057f246/download161dba7c75092df43a54c05c175eb605MD51TEXT13.pdf.txt13.pdf.txtExtracted texttext/plain34004https://repository.urosario.edu.co/bitstreams/dae0e068-8bfe-484d-92bd-c265bd284bfd/downloaddfc5d2ba589c7b50aff23c3151e807a4MD52THUMBNAIL13.pdf.jpg13.pdf.jpgGenerated Thumbnailimage/jpeg4689https://repository.urosario.edu.co/bitstreams/9348cc9d-c86f-42be-acbe-30ed645039de/download2685524d3e4187793c4b2af3ab3d6becMD5310336/22579oai:repository.urosario.edu.co:10336/225792022-05-02 07:37:20.500828https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae)
title Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae)
spellingShingle Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae)
Culex pipiens quinquefasciatus
Culicidae
Diptera
Hexapoda
Culture media
Embryonic tissues
Isoenzymatic patterns
Karyotype
RAPD-PCR
title_short Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae)
title_full Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae)
title_fullStr Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae)
title_full_unstemmed Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae)
title_sort Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae)
dc.subject.keyword.spa.fl_str_mv Culex pipiens quinquefasciatus
Culicidae
Diptera
Hexapoda
Culture media
Embryonic tissues
Isoenzymatic patterns
Karyotype
RAPD-PCR
topic Culex pipiens quinquefasciatus
Culicidae
Diptera
Hexapoda
Culture media
Embryonic tissues
Isoenzymatic patterns
Karyotype
RAPD-PCR
description Insect cell cultures are an important biotechnological tool for basic and applied studies. The objective of this work was to establish and characterise a new cell line from Culex quinquefasciatus embryonic tissues. Embryonated eggs were taken as a source of tissue to make explants that were seeded in L-15, Grace's, Grace's/L-15, MM/VP12, Schneider's and DMEM culture media with a pH range from 6.7-6.9 and incubated at 28°C. The morphological, cytogenetic, biochemical and molecular characteristics of the cell cultures were examined by observing the cell shapes, obtaining the karyotypes, using a cellulose-acetate electrophoretic system and performing random amplified polymorphic DNA-polymerase chain reaction analysis, respectively. The Grace's/L-15 medium provided the optimal nutritional conditions for cell adhesion and proliferation. Approximately 40-60 days following the explant procedure, a confluent monolayer was formed. Cellular morphology in the primary cultures and the subcultures was heterogeneous, but in the monolayer the epithelioid morphology type predominated. A karyotype with a diploid number of six chromosomes (2n = 6) was observed. Isoenzymatic and molecular patterns of the mosquito cell cultures matched those obtained from the immature and adult forms of the same species. Eighteen subcultures were generated. These cell cultures potentially constitute a useful tool for use in biomedical applications.
publishDate 2012
dc.date.created.spa.fl_str_mv 2012
dc.date.accessioned.none.fl_str_mv 2020-05-25T23:57:00Z
dc.date.available.none.fl_str_mv 2020-05-25T23:57:00Z
dc.type.eng.fl_str_mv article
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dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1590/S0074-02762012000100013
dc.identifier.issn.none.fl_str_mv 16788060
00740276
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url https://doi.org/10.1590/S0074-02762012000100013
https://repository.urosario.edu.co/handle/10336/22579
identifier_str_mv 16788060
00740276
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 95
dc.relation.citationIssue.none.fl_str_mv No. 1
dc.relation.citationStartPage.none.fl_str_mv 89
dc.relation.citationTitle.none.fl_str_mv Memorias do Instituto Oswaldo Cruz
dc.relation.citationVolume.none.fl_str_mv Vol. 107
dc.relation.ispartof.spa.fl_str_mv Memorias do Instituto Oswaldo Cruz, ISSN:16788060, 00740276, Vol.107, No.1 (2012); pp. 89-95
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dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
rights_invalid_str_mv Abierto (Texto Completo)
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dc.publisher.spa.fl_str_mv Fundacao Oswaldo Cruz
institution Universidad del Rosario
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dc.source.reponame.spa.fl_str_mv reponame:Repositorio Institucional EdocUR
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