Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae)
Insect cell cultures are an important biotechnological tool for basic and applied studies. The objective of this work was to establish and characterise a new cell line from Culex quinquefasciatus embryonic tissues. Embryonated eggs were taken as a source of tissue to make explants that were seeded i...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2012
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/22579
- Acceso en línea:
- https://doi.org/10.1590/S0074-02762012000100013
https://repository.urosario.edu.co/handle/10336/22579
- Palabra clave:
- Culex pipiens quinquefasciatus
Culicidae
Diptera
Hexapoda
Culture media
Embryonic tissues
Isoenzymatic patterns
Karyotype
RAPD-PCR
- Rights
- License
- Abierto (Texto Completo)
id |
EDOCUR2_6352221082682cab5d1d8d44a8431749 |
---|---|
oai_identifier_str |
oai:repository.urosario.edu.co:10336/22579 |
network_acronym_str |
EDOCUR2 |
network_name_str |
Repositorio EdocUR - U. Rosario |
repository_id_str |
|
spelling |
eaafab20-9002-4382-be1b-201f1742bec7-142fbddf0-7fbd-48cc-ae3d-009f774153c8-139d67619-1582-413c-a7da-7c3706659e05-1a00c7523-8e04-40b1-8983-a597d58eb894-12020-05-25T23:57:00Z2020-05-25T23:57:00Z2012Insect cell cultures are an important biotechnological tool for basic and applied studies. The objective of this work was to establish and characterise a new cell line from Culex quinquefasciatus embryonic tissues. Embryonated eggs were taken as a source of tissue to make explants that were seeded in L-15, Grace's, Grace's/L-15, MM/VP12, Schneider's and DMEM culture media with a pH range from 6.7-6.9 and incubated at 28°C. The morphological, cytogenetic, biochemical and molecular characteristics of the cell cultures were examined by observing the cell shapes, obtaining the karyotypes, using a cellulose-acetate electrophoretic system and performing random amplified polymorphic DNA-polymerase chain reaction analysis, respectively. The Grace's/L-15 medium provided the optimal nutritional conditions for cell adhesion and proliferation. Approximately 40-60 days following the explant procedure, a confluent monolayer was formed. Cellular morphology in the primary cultures and the subcultures was heterogeneous, but in the monolayer the epithelioid morphology type predominated. A karyotype with a diploid number of six chromosomes (2n = 6) was observed. Isoenzymatic and molecular patterns of the mosquito cell cultures matched those obtained from the immature and adult forms of the same species. Eighteen subcultures were generated. These cell cultures potentially constitute a useful tool for use in biomedical applications.application/pdfhttps://doi.org/10.1590/S0074-027620120001000131678806000740276https://repository.urosario.edu.co/handle/10336/22579engFundacao Oswaldo Cruz95No. 189Memorias do Instituto Oswaldo CruzVol. 107Memorias do Instituto Oswaldo Cruz, ISSN:16788060, 00740276, Vol.107, No.1 (2012); pp. 89-95https://www.scopus.com/inward/record.uri?eid=2-s2.0-84857099648&doi=10.1590%2fS0074-02762012000100013&partnerID=40&md5=95cc2f5423366c796bea1f24c5e7dfa2Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURCulex pipiens quinquefasciatusCulicidaeDipteraHexapodaCulture mediaEmbryonic tissuesIsoenzymatic patternsKaryotypeRAPD-PCREstablishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae)articleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Segura, Nidya ASantamaría, ErikaCabrera, Olga LBello, FelioORIGINAL13.pdfapplication/pdf675310https://repository.urosario.edu.co/bitstreams/103dd855-10cf-488d-bf45-622d7057f246/download161dba7c75092df43a54c05c175eb605MD51TEXT13.pdf.txt13.pdf.txtExtracted texttext/plain34004https://repository.urosario.edu.co/bitstreams/dae0e068-8bfe-484d-92bd-c265bd284bfd/downloaddfc5d2ba589c7b50aff23c3151e807a4MD52THUMBNAIL13.pdf.jpg13.pdf.jpgGenerated Thumbnailimage/jpeg4689https://repository.urosario.edu.co/bitstreams/9348cc9d-c86f-42be-acbe-30ed645039de/download2685524d3e4187793c4b2af3ab3d6becMD5310336/22579oai:repository.urosario.edu.co:10336/225792022-05-02 07:37:20.500828https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |
dc.title.spa.fl_str_mv |
Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae) |
title |
Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae) |
spellingShingle |
Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae) Culex pipiens quinquefasciatus Culicidae Diptera Hexapoda Culture media Embryonic tissues Isoenzymatic patterns Karyotype RAPD-PCR |
title_short |
Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae) |
title_full |
Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae) |
title_fullStr |
Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae) |
title_full_unstemmed |
Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae) |
title_sort |
Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae) |
dc.subject.keyword.spa.fl_str_mv |
Culex pipiens quinquefasciatus Culicidae Diptera Hexapoda Culture media Embryonic tissues Isoenzymatic patterns Karyotype RAPD-PCR |
topic |
Culex pipiens quinquefasciatus Culicidae Diptera Hexapoda Culture media Embryonic tissues Isoenzymatic patterns Karyotype RAPD-PCR |
description |
Insect cell cultures are an important biotechnological tool for basic and applied studies. The objective of this work was to establish and characterise a new cell line from Culex quinquefasciatus embryonic tissues. Embryonated eggs were taken as a source of tissue to make explants that were seeded in L-15, Grace's, Grace's/L-15, MM/VP12, Schneider's and DMEM culture media with a pH range from 6.7-6.9 and incubated at 28°C. The morphological, cytogenetic, biochemical and molecular characteristics of the cell cultures were examined by observing the cell shapes, obtaining the karyotypes, using a cellulose-acetate electrophoretic system and performing random amplified polymorphic DNA-polymerase chain reaction analysis, respectively. The Grace's/L-15 medium provided the optimal nutritional conditions for cell adhesion and proliferation. Approximately 40-60 days following the explant procedure, a confluent monolayer was formed. Cellular morphology in the primary cultures and the subcultures was heterogeneous, but in the monolayer the epithelioid morphology type predominated. A karyotype with a diploid number of six chromosomes (2n = 6) was observed. Isoenzymatic and molecular patterns of the mosquito cell cultures matched those obtained from the immature and adult forms of the same species. Eighteen subcultures were generated. These cell cultures potentially constitute a useful tool for use in biomedical applications. |
publishDate |
2012 |
dc.date.created.spa.fl_str_mv |
2012 |
dc.date.accessioned.none.fl_str_mv |
2020-05-25T23:57:00Z |
dc.date.available.none.fl_str_mv |
2020-05-25T23:57:00Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1590/S0074-02762012000100013 |
dc.identifier.issn.none.fl_str_mv |
16788060 00740276 |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/22579 |
url |
https://doi.org/10.1590/S0074-02762012000100013 https://repository.urosario.edu.co/handle/10336/22579 |
identifier_str_mv |
16788060 00740276 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.citationEndPage.none.fl_str_mv |
95 |
dc.relation.citationIssue.none.fl_str_mv |
No. 1 |
dc.relation.citationStartPage.none.fl_str_mv |
89 |
dc.relation.citationTitle.none.fl_str_mv |
Memorias do Instituto Oswaldo Cruz |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 107 |
dc.relation.ispartof.spa.fl_str_mv |
Memorias do Instituto Oswaldo Cruz, ISSN:16788060, 00740276, Vol.107, No.1 (2012); pp. 89-95 |
dc.relation.uri.spa.fl_str_mv |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84857099648&doi=10.1590%2fS0074-02762012000100013&partnerID=40&md5=95cc2f5423366c796bea1f24c5e7dfa2 |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.acceso.spa.fl_str_mv |
Abierto (Texto Completo) |
rights_invalid_str_mv |
Abierto (Texto Completo) http://purl.org/coar/access_right/c_abf2 |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Fundacao Oswaldo Cruz |
institution |
Universidad del Rosario |
dc.source.instname.spa.fl_str_mv |
instname:Universidad del Rosario |
dc.source.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional EdocUR |
bitstream.url.fl_str_mv |
https://repository.urosario.edu.co/bitstreams/103dd855-10cf-488d-bf45-622d7057f246/download https://repository.urosario.edu.co/bitstreams/dae0e068-8bfe-484d-92bd-c265bd284bfd/download https://repository.urosario.edu.co/bitstreams/9348cc9d-c86f-42be-acbe-30ed645039de/download |
bitstream.checksum.fl_str_mv |
161dba7c75092df43a54c05c175eb605 dfc5d2ba589c7b50aff23c3151e807a4 2685524d3e4187793c4b2af3ab3d6bec |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositorio institucional EdocUR |
repository.mail.fl_str_mv |
edocur@urosario.edu.co |
_version_ |
1814167736518967296 |