Clostridium perfringens in central Colombia
Clostridium perfringens is an opportunistic bacterium that causes intestinal diseases in both humans and animals. This study aimed to assess the frequency of C. perfringens and the presence of toxin-encoding genes in fecal samples from individuals with or without gastrointestinal symptoms in the Dep...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2024
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/44788
- Acceso en línea:
- https://doi.org/10.1186/s13099-024-00629-5
https://repository.urosario.edu.co/handle/10336/44788
- Palabra clave:
- Clostridium perfringens
Asymptomatic
Risk factors
Symptomatic
Toxins
- Rights
- License
- Attribution-NonCommercial-NoDerivatives 4.0 International
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Clostridium perfringens in central Colombia |
| title |
Clostridium perfringens in central Colombia |
| spellingShingle |
Clostridium perfringens in central Colombia Clostridium perfringens Asymptomatic Risk factors Symptomatic Toxins |
| title_short |
Clostridium perfringens in central Colombia |
| title_full |
Clostridium perfringens in central Colombia |
| title_fullStr |
Clostridium perfringens in central Colombia |
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Clostridium perfringens in central Colombia |
| title_sort |
Clostridium perfringens in central Colombia |
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Clostridium perfringens Asymptomatic Risk factors Symptomatic Toxins |
| topic |
Clostridium perfringens Asymptomatic Risk factors Symptomatic Toxins |
| description |
Clostridium perfringens is an opportunistic bacterium that causes intestinal diseases in both humans and animals. This study aimed to assess the frequency of C. perfringens and the presence of toxin-encoding genes in fecal samples from individuals with or without gastrointestinal symptoms in the Department of Boyacá, Colombia. Additionally, risk factors associated with carriage and disease development were analyzed. A total of 114 stool samples were analyzed using a molecular test based on specific polymerase chain reaction (PCR) targeting 16S-rRNA and alpha toxin (cpa) genes. For individuals with a positive result for the PCR test, stool samples were cultured on Tryptose Sulfite Cycloserine (TSC) agar. Two to five colonies forming units were selected based on phenotypic characteristics, resulting in 56 bacterial isolates. These isolates were then analyzed for toxin-coding genes associated with gastrointestinal diseases. In addition, sociodemographic and clinical data from 77 individuals were also analyzed. The overall frequency of C. perfringens was 19.3% (n = 22/114). The detection frequency in 77 individuals with clinical data was 16.6% (n = 5/30) among symptomatic individuals and 21.2% (n = 10/47) among asymptomatic individuals. All 56 isolates obtained carried the cpa gene, while cpb2 was present in 10.7% (n = 6/56) |
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2024 |
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2024-12-01 |
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2024-12-01 |
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2025-01-26T18:27:07Z |
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2025-01-26T18:27:07Z |
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Artículo |
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https://doi.org/10.1186/s13099-024-00629-5 |
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https://repository.urosario.edu.co/handle/10336/44788 |
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https://doi.org/10.1186/s13099-024-00629-5 https://repository.urosario.edu.co/handle/10336/44788 |
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eng |
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eng |
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Gut Pathogens |
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Attribution-NonCommercial-NoDerivatives 4.0 International |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ |
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Universidad del Rosario |
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3b7490f7-a153-437b-aba0-f5692e5bde6438fe4844-3164-49a3-9219-95f3a07525a075bcb2e2-f97d-41e5-9466-4518896df6c0516d76b8-e747-4740-9b32-1c71150bc93eaad90867-d450-4783-9c0f-e21f40eb741bca0a975a-3863-419e-9272-ed74450660e6b74e961a-5cde-44c1-ba49-217dc919af68c67d367e-96c2-4566-8a39-374ead1a65f610117161186007dac049d-c747-4b91-849c-c2efae4da8242025-01-26T18:27:07Z2025-01-26T18:27:07Z2024-12-012024-12-01Clostridium perfringens is an opportunistic bacterium that causes intestinal diseases in both humans and animals. This study aimed to assess the frequency of C. perfringens and the presence of toxin-encoding genes in fecal samples from individuals with or without gastrointestinal symptoms in the Department of Boyacá, Colombia. Additionally, risk factors associated with carriage and disease development were analyzed. A total of 114 stool samples were analyzed using a molecular test based on specific polymerase chain reaction (PCR) targeting 16S-rRNA and alpha toxin (cpa) genes. For individuals with a positive result for the PCR test, stool samples were cultured on Tryptose Sulfite Cycloserine (TSC) agar. Two to five colonies forming units were selected based on phenotypic characteristics, resulting in 56 bacterial isolates. These isolates were then analyzed for toxin-coding genes associated with gastrointestinal diseases. In addition, sociodemographic and clinical data from 77 individuals were also analyzed. The overall frequency of C. perfringens was 19.3% (n = 22/114). The detection frequency in 77 individuals with clinical data was 16.6% (n = 5/30) among symptomatic individuals and 21.2% (n = 10/47) among asymptomatic individuals. All 56 isolates obtained carried the cpa gene, while cpb2 was present in 10.7% (n = 6/56)cpe and cpb genes were not detected. Notably, diabetes and autoimmune diseases are significantly associated with an increased risk of C. perfringens detection (adjusted OR 8.41: 95% CI 1.32–35.89). This study highlights an elevated frequency of C. perfringens and the presence of the cpb2 gene in asymptomatic individuals compared with their symptomatic counterparts. These findings offer insights into the distribution and virulence factors of C. perfringens at a micro-geographical level. This information supports the need for developing tailored prevention strategies based on local characteristics to promote active surveillance programs based on molecular epidemiology.application/pdfhttps://doi.org/10.1186/s13099-024-00629-5https://repository.urosario.edu.co/handle/10336/44788engGut PathogensGut PathogensAttribution-NonCommercial-NoDerivatives 4.0 InternationalAbierto (Texto Completo)http://creativecommons.org/licenses/by-nc-sa/4.0/http://purl.org/coar/access_right/c_abf2Gut Pathogensinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURClostridium perfringensAsymptomaticRisk factorsSymptomaticToxinsClostridium perfringens in central ColombiaarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Camargo, AnnyBohórquez, LauraLópez, Diana PaolaFerrebuz-Cardozo, AtilioCastellanos‑Rozo, JoséDíaz-Ovalle, JavierRada, MarianaCamargo, MilenaRamírez González, Juan DavidMuñoz, MarinaORIGINALClostridium_perfringens_in_central_Colombia_frequency_toxin_genes_and_risk_factors.pdfapplication/pdf1175187https://repository.urosario.edu.co/bitstreams/cd551e4e-344a-4371-b48c-e640f604c007/downloade018a5e7b09f75318c021fa463fb3b4dMD51TEXTClostridium_perfringens_in_central_Colombia_frequency_toxin_genes_and_risk_factors.pdf.txtClostridium_perfringens_in_central_Colombia_frequency_toxin_genes_and_risk_factors.pdf.txtExtracted texttext/plain45022https://repository.urosario.edu.co/bitstreams/2fb23e8d-7f59-406a-b537-67d401f21046/download51d975233b19757e7ef5c67a3d7dc49eMD52THUMBNAILClostridium_perfringens_in_central_Colombia_frequency_toxin_genes_and_risk_factors.pdf.jpgClostridium_perfringens_in_central_Colombia_frequency_toxin_genes_and_risk_factors.pdf.jpgGenerated Thumbnailimage/jpeg4295https://repository.urosario.edu.co/bitstreams/22184b19-feb6-42df-88d3-1ae477051f3e/download4b7a1dc69d0ec047050e5000c9ed4610MD5310336/44788oai:repository.urosario.edu.co:10336/447882025-03-05 08:35:28.333http://creativecommons.org/licenses/by-nc-sa/4.0/Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttps://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |