Quantitative flow cytometric monitoring of invasion of epithelial cells by Mycobacterium tuberculosis

Current methods for evaluating mycobacterial invasion of target cells pose technical difficulties including a long turn-around time. Thus, new methodologies must be developed that allow rapid and reliable monitoring of host cell invasion. Here, the invasion of A549 cell line by SYBR safe-labeled Myc...

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Tipo de recurso:
Fecha de publicación:
2008
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/26834
Acceso en línea:
https://doi.org/10.2741/2709
https://repository.urosario.edu.co/handle/10336/26834
Palabra clave:
Mycobacterial invasion
Host cell
Mycobacterium tuberculosis
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License
Abierto (Texto Completo)
id EDOCUR2_575ea4fd32d6462100d3dabfa7e166cc
oai_identifier_str oai:repository.urosario.edu.co:10336/26834
network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
spelling 7ab10ad8-3da5-4c21-84e7-df1f0fb710a9-104c21c00-a97f-44e0-a84d-8869b0425802-13a0cc40a-2195-4f71-b1b6-76bdfe3757a8-1796530656002020-08-19T14:40:20Z2020-08-19T14:40:20Z2008-01-01Current methods for evaluating mycobacterial invasion of target cells pose technical difficulties including a long turn-around time. Thus, new methodologies must be developed that allow rapid and reliable monitoring of host cell invasion. Here, the invasion of A549 cell line by SYBR safe-labeled Mycobacterium tuberculosis (M. tuberculosis) H37Rv was assessed by flow cytometry and was expressed as the percentage of cells infected by M. tuberculosis. Over a third of A549 cells were invaded by M. tuberculosis, two hours after infection. The specificity of the invasion was confirmed in assays using red blood cells as target cells and Escherichia coli as the non-invasive bacterial control. These findings show that invasion of pulmonary epithelial cells by M. tuberculosis can rapidly and quantitatively be assessed with a great sensitivity by flow cytometric detection of SYBR safe-labeled M. tuberculosis.application/pdfhttps://doi.org/10.2741/2709ISSN: 1093-9946EISSN: 1093-4715https://repository.urosario.edu.co/handle/10336/26834engEuropa PMC656No. 3650Frontiers in Bioscience, Frontiers in Bioscience - LandmarkFrontiers in Bioscience, Frontiers in Bioscience - Landmark, ISSN: 1093-9946; EISSN: 1093-4715, No.3 (2008); pp. 650-656https://europepmc.org/article/med/17981577Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2Frontiers in Bioscience, Frontiers in Bioscience - Landmarkinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURMycobacterial invasionHost cellMycobacterium tuberculosisQuantitative flow cytometric monitoring of invasion of epithelial cells by Mycobacterium tuberculosisMonitorización cuantitativa por citometría de flujo de la invasión de células epiteliales por Mycobacterium tuberculosisarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Chapeton-Montes, JPlaza, D.FBarrero, C.APatarroyo, Manuel A.10336/26834oai:repository.urosario.edu.co:10336/268342021-06-03 00:50:00.495https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Quantitative flow cytometric monitoring of invasion of epithelial cells by Mycobacterium tuberculosis
dc.title.TranslatedTitle.spa.fl_str_mv Monitorización cuantitativa por citometría de flujo de la invasión de células epiteliales por Mycobacterium tuberculosis
title Quantitative flow cytometric monitoring of invasion of epithelial cells by Mycobacterium tuberculosis
spellingShingle Quantitative flow cytometric monitoring of invasion of epithelial cells by Mycobacterium tuberculosis
Mycobacterial invasion
Host cell
Mycobacterium tuberculosis
title_short Quantitative flow cytometric monitoring of invasion of epithelial cells by Mycobacterium tuberculosis
title_full Quantitative flow cytometric monitoring of invasion of epithelial cells by Mycobacterium tuberculosis
title_fullStr Quantitative flow cytometric monitoring of invasion of epithelial cells by Mycobacterium tuberculosis
title_full_unstemmed Quantitative flow cytometric monitoring of invasion of epithelial cells by Mycobacterium tuberculosis
title_sort Quantitative flow cytometric monitoring of invasion of epithelial cells by Mycobacterium tuberculosis
dc.subject.keyword.spa.fl_str_mv Mycobacterial invasion
Host cell
Mycobacterium tuberculosis
topic Mycobacterial invasion
Host cell
Mycobacterium tuberculosis
description Current methods for evaluating mycobacterial invasion of target cells pose technical difficulties including a long turn-around time. Thus, new methodologies must be developed that allow rapid and reliable monitoring of host cell invasion. Here, the invasion of A549 cell line by SYBR safe-labeled Mycobacterium tuberculosis (M. tuberculosis) H37Rv was assessed by flow cytometry and was expressed as the percentage of cells infected by M. tuberculosis. Over a third of A549 cells were invaded by M. tuberculosis, two hours after infection. The specificity of the invasion was confirmed in assays using red blood cells as target cells and Escherichia coli as the non-invasive bacterial control. These findings show that invasion of pulmonary epithelial cells by M. tuberculosis can rapidly and quantitatively be assessed with a great sensitivity by flow cytometric detection of SYBR safe-labeled M. tuberculosis.
publishDate 2008
dc.date.created.spa.fl_str_mv 2008-01-01
dc.date.accessioned.none.fl_str_mv 2020-08-19T14:40:20Z
dc.date.available.none.fl_str_mv 2020-08-19T14:40:20Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.2741/2709
dc.identifier.issn.none.fl_str_mv ISSN: 1093-9946
EISSN: 1093-4715
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/26834
url https://doi.org/10.2741/2709
https://repository.urosario.edu.co/handle/10336/26834
identifier_str_mv ISSN: 1093-9946
EISSN: 1093-4715
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 656
dc.relation.citationIssue.none.fl_str_mv No. 3
dc.relation.citationStartPage.none.fl_str_mv 650
dc.relation.citationTitle.none.fl_str_mv Frontiers in Bioscience, Frontiers in Bioscience - Landmark
dc.relation.ispartof.spa.fl_str_mv Frontiers in Bioscience, Frontiers in Bioscience - Landmark, ISSN: 1093-9946; EISSN: 1093-4715, No.3 (2008); pp. 650-656
dc.relation.uri.spa.fl_str_mv https://europepmc.org/article/med/17981577
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
rights_invalid_str_mv Abierto (Texto Completo)
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Europa PMC
dc.source.spa.fl_str_mv Frontiers in Bioscience, Frontiers in Bioscience - Landmark
institution Universidad del Rosario
dc.source.instname.none.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.none.fl_str_mv reponame:Repositorio Institucional EdocUR
repository.name.fl_str_mv Repositorio institucional EdocUR
repository.mail.fl_str_mv edocur@urosario.edu.co
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