Detection of Entamoeba histolytica by recombinase polymerase amplification
Amebiasis is an important cause of diarrheal disease worldwide and has been associated with childhood malnutrition. Traditional microscopy approaches are neither sensitive nor specific for Entamoeba histolytica. Antigen assays are more specific, but many cases are missed unless tested by molecular m...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2015
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/19086
- Acceso en línea:
- http://repository.urosario.edu.co/handle/10336/19086
- Palabra clave:
- Recombinase
Protozoal DNA
Recombinase
Amebiasis
Biochemical equipment
Controlled study
Diagnostic test
Diagnostic test accuracy study
Entamoeba
Entamoeba dispar
Enfermedades
Disentería amebiana
Entamoeba
Entamoeba histolytica
- Rights
- License
- Abierto (Texto completo)
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b35b15cd-3272-422f-8150-0c75a8fa72df-1fe1fc740-d7c9-40d8-922f-47c31e7bb094-16cfa230b-198f-47a3-bbff-283ede248ab1600c55ce785-da1b-41b4-ab2d-b1a4751fd418-16b59e0f3-b846-4db8-bb1d-3cc01f4154ec-1b7036fa7-1d07-495c-ae0b-6bd4263bc4e1-11011716118600f06fcd85-95ed-473f-bba2-1caed2af41a8-1f5f9e283-a635-44e3-96c9-79368de79abc-12019-02-15T17:19:14Z2019-02-15T17:19:14Z20152015Amebiasis is an important cause of diarrheal disease worldwide and has been associated with childhood malnutrition. Traditional microscopy approaches are neither sensitive nor specific for Entamoeba histolytica. Antigen assays are more specific, but many cases are missed unless tested by molecular methods. Although polymerase chain reaction (PCR) is effective, the need for sophisticated, expensive equipment, infrastructure, and trained personnel limits its usefulness, especially in the resource-limited, endemic areas. Here, we report development of a recombinase polymerase amplification (RPA) method to detect E. histolytica specifically. Using visual detection by lateral flow (LF), the test was highly sensitive and specific and could be performed without additional equipment. The availability of this inexpensive, sensitive, and field-applicable diagnostic test could facilitate rapid diagnosis and treatment of amebiasis in endemic regions. Copyright © 2015 by The American Society of Tropical Medicine and Hygiene.application/pdf10.4269/ajtmh.15-02760002-9637http://repository.urosario.edu.co/handle/10336/19086eng595No. 3591American Journal of Tropical Medicine and HygieneVol. 93American Journal of Tropical Medicine and Hygiene, ISSN: 0002-9637 Vol. 93, No. 3 (2015) pp. 591-595http://www.ajtmh.org/docserver/fulltext/14761645/93/3/591.pdf?expires=1544545793&id=id&accname=guest&checksum=FFD734F21CCF67D5AEC1F47F5ADC65C6Abierto (Texto completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURRecombinaseProtozoal DNARecombinaseAmebiasisBiochemical equipmentControlled studyDiagnostic testDiagnostic test accuracy studyEntamoebaEntamoeba disparEnfermedades616600Disentería amebianaEntamoebaEntamoeba histolyticaDetection of Entamoeba histolytica by recombinase polymerase amplificationarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Nair, GayatriRebolledo, MauricioClinton White, A.Crannell, ZacharyRichards-Kortum, R. RebeccaPinilla, A. ElizabethRamírez, Juan DavidLópez, M. ConsueloCastellanos-Gonzalez, AlejandroNair, GayatriRebolledo, MauricioClinton White, A.Crannell, ZacharyRichards-Kortum, R. RebeccaPinilla, A. ElizabethRamírez, Juan DavidLópez, M. ConsueloCastellanos-Gonzalez, AlejandroORIGINALDetection_of_entamoeba_histolytica_by_recombinase_polymerase_amplification.pdfapplication/pdf646787https://repository.urosario.edu.co/bitstreams/25f2aa21-8603-4358-8cad-af035b730bcf/download660a3c841ed6dc51838a11a2b65c8774MD51TEXTDetection_of_entamoeba_histolytica_by_recombinase_polymerase_amplification.pdf.txtDetection_of_entamoeba_histolytica_by_recombinase_polymerase_amplification.pdf.txtExtracted texttext/plain27292https://repository.urosario.edu.co/bitstreams/73298863-7bfa-4067-8e49-47dff7f428c7/download5825ef4b868e918b37b7dac4625e2ecaMD52THUMBNAILDetection_of_entamoeba_histolytica_by_recombinase_polymerase_amplification.pdf.jpgDetection_of_entamoeba_histolytica_by_recombinase_polymerase_amplification.pdf.jpgGenerated Thumbnailimage/jpeg4459https://repository.urosario.edu.co/bitstreams/74f9aa14-5661-44d8-985c-99237f82cb59/downloade368d956a48d927f16cc639a4500621fMD5310336/19086oai:repository.urosario.edu.co:10336/190862021-06-03 00:49:17.331https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |
dc.title.spa.fl_str_mv |
Detection of Entamoeba histolytica by recombinase polymerase amplification |
title |
Detection of Entamoeba histolytica by recombinase polymerase amplification |
spellingShingle |
Detection of Entamoeba histolytica by recombinase polymerase amplification Recombinase Protozoal DNA Recombinase Amebiasis Biochemical equipment Controlled study Diagnostic test Diagnostic test accuracy study Entamoeba Entamoeba dispar Enfermedades Disentería amebiana Entamoeba Entamoeba histolytica |
title_short |
Detection of Entamoeba histolytica by recombinase polymerase amplification |
title_full |
Detection of Entamoeba histolytica by recombinase polymerase amplification |
title_fullStr |
Detection of Entamoeba histolytica by recombinase polymerase amplification |
title_full_unstemmed |
Detection of Entamoeba histolytica by recombinase polymerase amplification |
title_sort |
Detection of Entamoeba histolytica by recombinase polymerase amplification |
dc.subject.spa.fl_str_mv |
Recombinase Protozoal DNA Recombinase Amebiasis Biochemical equipment Controlled study Diagnostic test Diagnostic test accuracy study Entamoeba Entamoeba dispar |
topic |
Recombinase Protozoal DNA Recombinase Amebiasis Biochemical equipment Controlled study Diagnostic test Diagnostic test accuracy study Entamoeba Entamoeba dispar Enfermedades Disentería amebiana Entamoeba Entamoeba histolytica |
dc.subject.ddc.none.fl_str_mv |
Enfermedades |
dc.subject.lemb.spa.fl_str_mv |
Disentería amebiana Entamoeba Entamoeba histolytica |
description |
Amebiasis is an important cause of diarrheal disease worldwide and has been associated with childhood malnutrition. Traditional microscopy approaches are neither sensitive nor specific for Entamoeba histolytica. Antigen assays are more specific, but many cases are missed unless tested by molecular methods. Although polymerase chain reaction (PCR) is effective, the need for sophisticated, expensive equipment, infrastructure, and trained personnel limits its usefulness, especially in the resource-limited, endemic areas. Here, we report development of a recombinase polymerase amplification (RPA) method to detect E. histolytica specifically. Using visual detection by lateral flow (LF), the test was highly sensitive and specific and could be performed without additional equipment. The availability of this inexpensive, sensitive, and field-applicable diagnostic test could facilitate rapid diagnosis and treatment of amebiasis in endemic regions. Copyright © 2015 by The American Society of Tropical Medicine and Hygiene. |
publishDate |
2015 |
dc.date.created.spa.fl_str_mv |
2015 |
dc.date.issued.none.fl_str_mv |
2015 |
dc.date.accessioned.none.fl_str_mv |
2019-02-15T17:19:14Z |
dc.date.available.none.fl_str_mv |
2019-02-15T17:19:14Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.none.fl_str_mv |
10.4269/ajtmh.15-0276 |
dc.identifier.issn.none.fl_str_mv |
0002-9637 |
dc.identifier.uri.none.fl_str_mv |
http://repository.urosario.edu.co/handle/10336/19086 |
identifier_str_mv |
10.4269/ajtmh.15-0276 0002-9637 |
url |
http://repository.urosario.edu.co/handle/10336/19086 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.citationEndPage.none.fl_str_mv |
595 |
dc.relation.citationIssue.none.fl_str_mv |
No. 3 |
dc.relation.citationStartPage.none.fl_str_mv |
591 |
dc.relation.citationTitle.none.fl_str_mv |
American Journal of Tropical Medicine and Hygiene |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 93 |
dc.relation.ispartof.spa.fl_str_mv |
American Journal of Tropical Medicine and Hygiene, ISSN: 0002-9637 Vol. 93, No. 3 (2015) pp. 591-595 |
dc.relation.uri.spa.fl_str_mv |
http://www.ajtmh.org/docserver/fulltext/14761645/93/3/591.pdf?expires=1544545793&id=id&accname=guest&checksum=FFD734F21CCF67D5AEC1F47F5ADC65C6 |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.acceso.spa.fl_str_mv |
Abierto (Texto completo) |
rights_invalid_str_mv |
Abierto (Texto completo) http://purl.org/coar/access_right/c_abf2 |
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application/pdf |
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Universidad del Rosario |
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