Detection of Entamoeba histolytica by recombinase polymerase amplification

Amebiasis is an important cause of diarrheal disease worldwide and has been associated with childhood malnutrition. Traditional microscopy approaches are neither sensitive nor specific for Entamoeba histolytica. Antigen assays are more specific, but many cases are missed unless tested by molecular m...

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Autores:
Tipo de recurso:
Fecha de publicación:
2015
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/19086
Acceso en línea:
http://repository.urosario.edu.co/handle/10336/19086
Palabra clave:
Recombinase
Protozoal DNA
Recombinase
Amebiasis
Biochemical equipment
Controlled study
Diagnostic test
Diagnostic test accuracy study
Entamoeba
Entamoeba dispar
Enfermedades
Disentería amebiana
Entamoeba
Entamoeba histolytica
Rights
License
Abierto (Texto completo)
Description
Summary:Amebiasis is an important cause of diarrheal disease worldwide and has been associated with childhood malnutrition. Traditional microscopy approaches are neither sensitive nor specific for Entamoeba histolytica. Antigen assays are more specific, but many cases are missed unless tested by molecular methods. Although polymerase chain reaction (PCR) is effective, the need for sophisticated, expensive equipment, infrastructure, and trained personnel limits its usefulness, especially in the resource-limited, endemic areas. Here, we report development of a recombinase polymerase amplification (RPA) method to detect E. histolytica specifically. Using visual detection by lateral flow (LF), the test was highly sensitive and specific and could be performed without additional equipment. The availability of this inexpensive, sensitive, and field-applicable diagnostic test could facilitate rapid diagnosis and treatment of amebiasis in endemic regions. Copyright © 2015 by The American Society of Tropical Medicine and Hygiene.