Análisis comparativo de las respuestas transcripcionales de cinco especies de Leishmania frente al antimonio trivalente.
La leishmaniasis es considerada una enfermedad tropical desatendida para la cual no se cuenta con una vacuna. Por otra parte, las drogas de primera elección han presentado un aumento en las fallas terapéuticas, entre otras causas por la adquisición de resistencia por parte de su agente etiológico de...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2021
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- spa
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/30923
- Acceso en línea:
- https://doi.org/10.48713/10336_30923
https://repository.urosario.edu.co/handle/10336/30923
- Palabra clave:
- Leishmania donovani
Leishmania infantum
Leishmania amazonensis
Leishmania Viannia
Leishmania panamensis
Leishmania braziliensis
Efectividad del Antimonio trivalente (SbIII) frente leishmania
Genómica y transcriptómica comparativa de Leishmania
Perfil transcriptómico de leishmania
Microbiología
Leishmania donovani
Leishmania infantum
Leishmania amazonensis
Leishmania Viannia
Leishmania panamensis
Leishmania braziliensis
Effectiveness of trivalent antimony (SbIII) against leishmania
Comparative genomics and transcriptomics of Leishmania
Leishmania transcriptomic profile
- Rights
- License
- Atribución-SinDerivadas 2.5 Colombia
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EDOCUR2_4c8a25816de6e495fcc736f7ca40a72f |
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oai:repository.urosario.edu.co:10336/30923 |
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EDOCUR2 |
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Repositorio EdocUR - U. Rosario |
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|
| dc.title.spa.fl_str_mv |
Análisis comparativo de las respuestas transcripcionales de cinco especies de Leishmania frente al antimonio trivalente. |
| dc.title.TranslatedTitle.eng.fl_str_mv |
Comparative analysis of the transcriptional responses of five Leishmania species against trivalent antimony. |
| title |
Análisis comparativo de las respuestas transcripcionales de cinco especies de Leishmania frente al antimonio trivalente. |
| spellingShingle |
Análisis comparativo de las respuestas transcripcionales de cinco especies de Leishmania frente al antimonio trivalente. Leishmania donovani Leishmania infantum Leishmania amazonensis Leishmania Viannia Leishmania panamensis Leishmania braziliensis Efectividad del Antimonio trivalente (SbIII) frente leishmania Genómica y transcriptómica comparativa de Leishmania Perfil transcriptómico de leishmania Microbiología Leishmania donovani Leishmania infantum Leishmania amazonensis Leishmania Viannia Leishmania panamensis Leishmania braziliensis Effectiveness of trivalent antimony (SbIII) against leishmania Comparative genomics and transcriptomics of Leishmania Leishmania transcriptomic profile |
| title_short |
Análisis comparativo de las respuestas transcripcionales de cinco especies de Leishmania frente al antimonio trivalente. |
| title_full |
Análisis comparativo de las respuestas transcripcionales de cinco especies de Leishmania frente al antimonio trivalente. |
| title_fullStr |
Análisis comparativo de las respuestas transcripcionales de cinco especies de Leishmania frente al antimonio trivalente. |
| title_full_unstemmed |
Análisis comparativo de las respuestas transcripcionales de cinco especies de Leishmania frente al antimonio trivalente. |
| title_sort |
Análisis comparativo de las respuestas transcripcionales de cinco especies de Leishmania frente al antimonio trivalente. |
| dc.contributor.advisor.none.fl_str_mv |
Ramírez, Juan David |
| dc.subject.spa.fl_str_mv |
Leishmania donovani Leishmania infantum Leishmania amazonensis Leishmania Viannia Leishmania panamensis Leishmania braziliensis Efectividad del Antimonio trivalente (SbIII) frente leishmania Genómica y transcriptómica comparativa de Leishmania Perfil transcriptómico de leishmania |
| topic |
Leishmania donovani Leishmania infantum Leishmania amazonensis Leishmania Viannia Leishmania panamensis Leishmania braziliensis Efectividad del Antimonio trivalente (SbIII) frente leishmania Genómica y transcriptómica comparativa de Leishmania Perfil transcriptómico de leishmania Microbiología Leishmania donovani Leishmania infantum Leishmania amazonensis Leishmania Viannia Leishmania panamensis Leishmania braziliensis Effectiveness of trivalent antimony (SbIII) against leishmania Comparative genomics and transcriptomics of Leishmania Leishmania transcriptomic profile |
| dc.subject.ddc.spa.fl_str_mv |
Microbiología |
| dc.subject.keyword.spa.fl_str_mv |
Leishmania donovani Leishmania infantum Leishmania amazonensis Leishmania Viannia Leishmania panamensis Leishmania braziliensis Effectiveness of trivalent antimony (SbIII) against leishmania Comparative genomics and transcriptomics of Leishmania Leishmania transcriptomic profile |
| description |
La leishmaniasis es considerada una enfermedad tropical desatendida para la cual no se cuenta con una vacuna. Por otra parte, las drogas de primera elección han presentado un aumento en las fallas terapéuticas, entre otras causas por la adquisición de resistencia por parte de su agente etiológico dependiente de las características propias de cada especie (ej. manifestación clínica y distribución geográfica). Así, que comprender el mecanismo usado por el parásito para sobrevivir bajo la presión de tratamientos identificando probables blancos terapéuticos comunes y específicos es importante para el control de la leishmaniasis. Sin embargo, hasta el momento no se ha realizado un análisis donde se compare la expresión génica entre especies de Leishmania que exhiben diferentes características genéticas y biológicas reflejadas en las manifestaciones clínicas diferenciales asociadas. Aquí, aplicamos análisis comparativos de los perfiles transcriptómicos de líneas con resistencia inducida experimentalmente al antimonio trivalente (SbIII) de cinco especies de importancia médica (Subgénero L. (Leishmania): L. donovani, L. infantum y L. amazonensis; Subgénero L. (Viannia): L. panamensis y L. braziliensis), causantes de diferentes manifestaciones clínicas (que generalmente son Leishmaniasis cutánea para L. panamensis y L. amazonensis, mucocutánea para L. braziliensis y visceral para L. donovani y L. infantum) a partir de análisis funcionales de ontología y asignación de grupos ortólogos. Las líneas resistentes tenían respuestas diferenciales principalmente en procesos metabólicos, unión a compuestos y componentes membranales respecto a su contraparte sensible. Predominaron los genes ortólogos diferencialmente expresados asignados a las respuestas especie especificas con un total de 1426 genes propios. A nivel de la respuesta por subgénero no se encontraron genes compartidos entre las especies pertenecientes a L. (Leishmania) y solo 7 lo fueron entre aquellas pertenecientes a L. (Viannia). No se halló ningún gen diferencialmente expresado en común entre las 5 especies, pero se encontraron dos genes ortólogos sobrerregulados comunes entre 4 especies (L. donovani, L. braziliensis, L. amazonensis y L. panamensis) referidos a una proteína de unión a RNA y al complejo NAD(P)H citocromo B5 oxidorreductasa, asociados al control transcripcional y a la síntesis de novo del ácido linoleico, importantes en los mecanismos de resistencia a los antimoniales. Estos patrones obedecen probablemente el fenómeno multifactorial de la resistencia a drogas, dependiente de características intrínsecas del parásito y el entorno. Por ende, las aproximaciones especie específicas resultan más recomendables para la proposición de potenciales blancos terapéuticos. |
| publishDate |
2021 |
| dc.date.accessioned.none.fl_str_mv |
2021-02-16T23:44:21Z |
| dc.date.available.none.fl_str_mv |
2021-02-16T23:44:21Z |
| dc.date.created.none.fl_str_mv |
2021-01-25 |
| dc.type.eng.fl_str_mv |
bachelorThesis |
| dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_7a1f |
| dc.type.document.spa.fl_str_mv |
Artículo |
| dc.type.spa.spa.fl_str_mv |
Trabajo de grado |
| dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.48713/10336_30923 |
| dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/30923 |
| url |
https://doi.org/10.48713/10336_30923 https://repository.urosario.edu.co/handle/10336/30923 |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.rights.spa.fl_str_mv |
Atribución-SinDerivadas 2.5 Colombia |
| dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
| dc.rights.acceso.spa.fl_str_mv |
Abierto (Texto Completo) |
| dc.rights.uri.none.fl_str_mv |
http://creativecommons.org/licenses/by-nd/2.5/co/ |
| rights_invalid_str_mv |
Atribución-SinDerivadas 2.5 Colombia Abierto (Texto Completo) http://creativecommons.org/licenses/by-nd/2.5/co/ http://purl.org/coar/access_right/c_abf2 |
| dc.format.mimetype.none.fl_str_mv |
application/pdf |
| dc.publisher.spa.fl_str_mv |
Universidad del Rosario |
| dc.publisher.department.spa.fl_str_mv |
Facultad de Ciencias Naturales y Matemáticas |
| dc.publisher.program.spa.fl_str_mv |
Biología |
| institution |
Universidad del Rosario |
| dc.source.bibliographicCitation.spa.fl_str_mv |
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Ramírez, Juan David1011716118600Medina Velasquez, Julián EstebanBiólogoFull timef12cac92-fc6f-4b21-a221-fbb43a1641956002021-02-16T23:44:21Z2021-02-16T23:44:21Z2021-01-25La leishmaniasis es considerada una enfermedad tropical desatendida para la cual no se cuenta con una vacuna. Por otra parte, las drogas de primera elección han presentado un aumento en las fallas terapéuticas, entre otras causas por la adquisición de resistencia por parte de su agente etiológico dependiente de las características propias de cada especie (ej. manifestación clínica y distribución geográfica). Así, que comprender el mecanismo usado por el parásito para sobrevivir bajo la presión de tratamientos identificando probables blancos terapéuticos comunes y específicos es importante para el control de la leishmaniasis. Sin embargo, hasta el momento no se ha realizado un análisis donde se compare la expresión génica entre especies de Leishmania que exhiben diferentes características genéticas y biológicas reflejadas en las manifestaciones clínicas diferenciales asociadas. Aquí, aplicamos análisis comparativos de los perfiles transcriptómicos de líneas con resistencia inducida experimentalmente al antimonio trivalente (SbIII) de cinco especies de importancia médica (Subgénero L. (Leishmania): L. donovani, L. infantum y L. amazonensis; Subgénero L. (Viannia): L. panamensis y L. braziliensis), causantes de diferentes manifestaciones clínicas (que generalmente son Leishmaniasis cutánea para L. panamensis y L. amazonensis, mucocutánea para L. braziliensis y visceral para L. donovani y L. infantum) a partir de análisis funcionales de ontología y asignación de grupos ortólogos. Las líneas resistentes tenían respuestas diferenciales principalmente en procesos metabólicos, unión a compuestos y componentes membranales respecto a su contraparte sensible. Predominaron los genes ortólogos diferencialmente expresados asignados a las respuestas especie especificas con un total de 1426 genes propios. A nivel de la respuesta por subgénero no se encontraron genes compartidos entre las especies pertenecientes a L. (Leishmania) y solo 7 lo fueron entre aquellas pertenecientes a L. (Viannia). No se halló ningún gen diferencialmente expresado en común entre las 5 especies, pero se encontraron dos genes ortólogos sobrerregulados comunes entre 4 especies (L. donovani, L. braziliensis, L. amazonensis y L. panamensis) referidos a una proteína de unión a RNA y al complejo NAD(P)H citocromo B5 oxidorreductasa, asociados al control transcripcional y a la síntesis de novo del ácido linoleico, importantes en los mecanismos de resistencia a los antimoniales. Estos patrones obedecen probablemente el fenómeno multifactorial de la resistencia a drogas, dependiente de características intrínsecas del parásito y el entorno. Por ende, las aproximaciones especie específicas resultan más recomendables para la proposición de potenciales blancos terapéuticos.Leishmaniasis is considered a neglected tropical disease for which there is no vaccine. On the other hand, first-line drugs have shown an increase in therapeutic failures, among other causes due to the acquisition of resistance by their etiological agent, depending on the characteristics of each species (eg, clinical manifestation and geographic distribution). Thus, understanding the mechanism used by the parasite to survive under the pressure of treatments by identifying probable common and specific therapeutic targets is important for the control of leishmaniasis. However, to date no analysis has been performed comparing gene expression between Leishmania species that exhibit different genetic and biological characteristics reflected in the associated differential clinical manifestations. Here, we apply comparative analyzes of the transcriptomic profiles of lines with experimentally induced resistance to trivalent antimony (SbIII) of five species of medical importance (Subgenus L. (Leishmania): L. donovani, L. infantum and L. amazonensis; Subgenus L. (Viannia): L. panamensis and L. braziliensis), causing different clinical manifestations (which are generally cutaneous Leishmaniasis for L. panamensis and L. amazonensis, mucocutaneous for L. braziliensis and visceral for L. donovani and L. infantum) a starting from functional analysis of ontology and assignment of orthologous groups. The resistant lines had differential responses mainly in metabolic processes, compound binding and membrane components with respect to their sensitive counterpart. metabolic, binding to membrane compounds and components relative to their sensitive counterpart. Differentially expressed orthologous genes assigned to species-specific responses predominated with a total of 1426 self genes. At the level of the response by subgenus, no shared genes were found among the species belonging to L. (Leishmania) and only 7 were found among those belonging to L. (Viannia). No differentially expressed gene was found in common among the 5 species, but two common upregulated orthologous genes were found among 4 species (L. donovani, L. braziliensis, L. amazonensis and L. panamensis) referred to an RNA-binding protein and the NAD (P) H cytochrome B5 oxidoreductase complex, associated with transcriptional control and de novo synthesis of linoleic acid, important in the mechanisms of resistance to antimonials. These patterns probably obey the multifactorial phenomenon of drug resistance, dependent on the intrinsic characteristics of the parasite and the environment. Therefore, species-specific approaches are more advisable for proposing potential therapeutic targets.application/pdfhttps://doi.org/10.48713/10336_30923 https://repository.urosario.edu.co/handle/10336/30923spaUniversidad del RosarioFacultad de Ciencias Naturales y MatemáticasBiologíaAtribución-SinDerivadas 2.5 ColombiaAbierto (Texto Completo)EL AUTOR, manifiesta que la obra objeto de la presente autorización es original y la realizó sin violar o usurpar derechos de autor de terceros, por lo tanto la obra es de exclusiva autoría y tiene la titularidad sobre la misma.http://creativecommons.org/licenses/by-nd/2.5/co/http://purl.org/coar/access_right/c_abf2Acino Brettmann, E. (2017). The Role of RNA Interference in the Control of Leishmania RNA virus 1 Infection. Retrieved from https://openscholarship.wustl.edu/art_sci_etds/1090Akhoundi, M., Kuhls, K., Cannet, A., Votýpka, J., Marty, P., Delaunay, P., & Sereno, D. (2016). 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