Specific erythrocyte binding capacity and biological activity of plasmodium falciparum-derived rhoptry-associated protein 1 peptides

Rhoptry-associated protein 1 (RAP1) is a merozoite antigen within Plasmodium falciparum rhoptries as yet having no specific function described for it. Synthetic peptides spanning the RAP1 sequence were tested in erythrocyte binding assays to identify possible RAP1 functional regions. Five high activ...

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Autores:
Tipo de recurso:
Fecha de publicación:
2004
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/26988
Acceso en línea:
https://doi.org/10.1016/j.vaccine.2003.07.019
https://repository.urosario.edu.co/handle/10336/26988
Palabra clave:
RAP1
Peptides
Malaria
Rights
License
Restringido (Acceso a grupos específicos)
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oai_identifier_str oai:repository.urosario.edu.co:10336/26988
network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
spelling 91225589-1fa33b517-50cf-4bb5-a980-3773caf84cd8-15e82e691-ba88-4d28-b934-b924d1350834-1c558796b-4d23-4bf9-b8b3-7d818293f920-166a49df9-ed6d-49a8-8822-34997a9c17e5-134b82e95-53e0-4ba5-a5c5-d4191164afa1-164e2fd99-86b7-4e34-9d38-c4ae6270a272-1efc03fc3-85c7-4fd4-b781-5082e60208ef-1f2d05f42-2948-4c51-8389-8c3817c2d0f1-11450e86b-c3ae-413a-bffa-4caa6fae204a-14272869d-a644-44e9-a7a4-9c26d936bb9e-110ecd4f9-843f-4ef2-bec0-7d39d3381a13-1518488266002020-08-19T14:40:41Z2020-08-19T14:40:41Z2004-02-25Rhoptry-associated protein 1 (RAP1) is a merozoite antigen within Plasmodium falciparum rhoptries as yet having no specific function described for it. Synthetic peptides spanning the RAP1 sequence were tested in erythrocyte binding assays to identify possible RAP1 functional regions. Five high activity binding peptides (HABPs) were identified; 26201, 26202, 26203 and 26204 spanned residues –K540 within RAP1 Cys region, whilst 26188 (–Y220) was located in p67 amino terminal. The results showed that peptide binding was saturable, some HABPs inhibited in vitro merozoite invasion and specifically bound to a 72 kDa protein in red blood cell membrane. HABP possible function in merozoite invasion of erythrocytes is also discussed.application/pdfhttps://doi.org/10.1016/j.vaccine.2003.07.019ISSN: 0264-410XEISSN: 1873-2518https://repository.urosario.edu.co/handle/10336/26988engThe Japanese Society for VaccinologyElsevier1062No. 81054VaccineVol. 22Vaccine, ISSN: 0264-410X;EISSN: 1873-2518, Vol.22, No.8 (2004); pp. 1054-1062 https://www.sciencedirect.com/science/article/pii/S0264410X03006625?via%3DihubRestringido (Acceso a grupos específicos)http://purl.org/coar/access_right/c_16ecVaccineinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURRAP1PeptidesMalariaSpecific erythrocyte binding capacity and biological activity of plasmodium falciparum-derived rhoptry-associated protein 1 peptidesCapacidad de unión de eritrocitos específica y actividad biológica de péptidos de proteína 1 asociados a rhoptry derivados de Plasmodium falciparumarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Curtidor, HernandoTovar, DianaLópez, RamsesGarc??a, JavierValbuena, JhonVera, RicardoSuárez, JorgeRodr??guez, Luis E.Puentes, ÁlvaroGuzmán, FannyTorres, ElizabethPatarroyo, Manuel E.Ocampo, Marisol10336/26988oai:repository.urosario.edu.co:10336/269882022-05-02 07:37:13.509112https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Specific erythrocyte binding capacity and biological activity of plasmodium falciparum-derived rhoptry-associated protein 1 peptides
dc.title.TranslatedTitle.spa.fl_str_mv Capacidad de unión de eritrocitos específica y actividad biológica de péptidos de proteína 1 asociados a rhoptry derivados de Plasmodium falciparum
title Specific erythrocyte binding capacity and biological activity of plasmodium falciparum-derived rhoptry-associated protein 1 peptides
spellingShingle Specific erythrocyte binding capacity and biological activity of plasmodium falciparum-derived rhoptry-associated protein 1 peptides
RAP1
Peptides
Malaria
title_short Specific erythrocyte binding capacity and biological activity of plasmodium falciparum-derived rhoptry-associated protein 1 peptides
title_full Specific erythrocyte binding capacity and biological activity of plasmodium falciparum-derived rhoptry-associated protein 1 peptides
title_fullStr Specific erythrocyte binding capacity and biological activity of plasmodium falciparum-derived rhoptry-associated protein 1 peptides
title_full_unstemmed Specific erythrocyte binding capacity and biological activity of plasmodium falciparum-derived rhoptry-associated protein 1 peptides
title_sort Specific erythrocyte binding capacity and biological activity of plasmodium falciparum-derived rhoptry-associated protein 1 peptides
dc.subject.keyword.spa.fl_str_mv RAP1
Peptides
Malaria
topic RAP1
Peptides
Malaria
description Rhoptry-associated protein 1 (RAP1) is a merozoite antigen within Plasmodium falciparum rhoptries as yet having no specific function described for it. Synthetic peptides spanning the RAP1 sequence were tested in erythrocyte binding assays to identify possible RAP1 functional regions. Five high activity binding peptides (HABPs) were identified; 26201, 26202, 26203 and 26204 spanned residues –K540 within RAP1 Cys region, whilst 26188 (–Y220) was located in p67 amino terminal. The results showed that peptide binding was saturable, some HABPs inhibited in vitro merozoite invasion and specifically bound to a 72 kDa protein in red blood cell membrane. HABP possible function in merozoite invasion of erythrocytes is also discussed.
publishDate 2004
dc.date.created.spa.fl_str_mv 2004-02-25
dc.date.accessioned.none.fl_str_mv 2020-08-19T14:40:41Z
dc.date.available.none.fl_str_mv 2020-08-19T14:40:41Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1016/j.vaccine.2003.07.019
dc.identifier.issn.none.fl_str_mv ISSN: 0264-410X
EISSN: 1873-2518
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/26988
url https://doi.org/10.1016/j.vaccine.2003.07.019
https://repository.urosario.edu.co/handle/10336/26988
identifier_str_mv ISSN: 0264-410X
EISSN: 1873-2518
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 1062
dc.relation.citationIssue.none.fl_str_mv No. 8
dc.relation.citationStartPage.none.fl_str_mv 1054
dc.relation.citationTitle.none.fl_str_mv Vaccine
dc.relation.citationVolume.none.fl_str_mv Vol. 22
dc.relation.ispartof.spa.fl_str_mv Vaccine, ISSN: 0264-410X;EISSN: 1873-2518, Vol.22, No.8 (2004); pp. 1054-1062
dc.relation.uri.spa.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0264410X03006625?via%3Dihub
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_16ec
dc.rights.acceso.spa.fl_str_mv Restringido (Acceso a grupos específicos)
rights_invalid_str_mv Restringido (Acceso a grupos específicos)
http://purl.org/coar/access_right/c_16ec
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv The Japanese Society for Vaccinology
Elsevier
dc.source.spa.fl_str_mv Vaccine
institution Universidad del Rosario
dc.source.instname.none.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.none.fl_str_mv reponame:Repositorio Institucional EdocUR
repository.name.fl_str_mv Repositorio institucional EdocUR
repository.mail.fl_str_mv edocur@urosario.edu.co
_version_ 1814167591650852864

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