The diagnosis of fungal neglected tropical diseases (fungal NTDs) and the role of investigation and laboratory tests: An expert consensus report

The diagnosis of fungal Neglected Tropical Diseases (NTD) is primarily based on initial visual recognition of a suspected case followed by confirmatory laboratory testing, which is often limited to specialized facilities. Although molecular and serodiagnostic tools have advanced, a substantial gap r...

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Autores:
Tipo de recurso:
Fecha de publicación:
2019
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/23397
Acceso en línea:
https://doi.org/10.3390/tropicalmed4040122
https://repository.urosario.edu.co/handle/10336/23397
Palabra clave:
Impression material
Article
Biopsy
Chromomycosis
Consensus
Fungus culture
Health care utilization
Histopathology
Human
Laboratory diagnosis
Laboratory test
Microscopy
Mycetoma
Mycosis
Neglected disease
Nonhuman
Patient referral
Point of care testing
Sporotrichosis
Tropical disease
Chromoblastomycosis
Fungal ntds
Integrated approaches
Laboratory diagnosis
Mycetoma
Sporotrichosis
Rights
License
Abierto (Texto Completo)
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dc.title.spa.fl_str_mv The diagnosis of fungal neglected tropical diseases (fungal NTDs) and the role of investigation and laboratory tests: An expert consensus report
title The diagnosis of fungal neglected tropical diseases (fungal NTDs) and the role of investigation and laboratory tests: An expert consensus report
spellingShingle The diagnosis of fungal neglected tropical diseases (fungal NTDs) and the role of investigation and laboratory tests: An expert consensus report
Impression material
Article
Biopsy
Chromomycosis
Consensus
Fungus culture
Health care utilization
Histopathology
Human
Laboratory diagnosis
Laboratory test
Microscopy
Mycetoma
Mycosis
Neglected disease
Nonhuman
Patient referral
Point of care testing
Sporotrichosis
Tropical disease
Chromoblastomycosis
Fungal ntds
Integrated approaches
Laboratory diagnosis
Mycetoma
Sporotrichosis
title_short The diagnosis of fungal neglected tropical diseases (fungal NTDs) and the role of investigation and laboratory tests: An expert consensus report
title_full The diagnosis of fungal neglected tropical diseases (fungal NTDs) and the role of investigation and laboratory tests: An expert consensus report
title_fullStr The diagnosis of fungal neglected tropical diseases (fungal NTDs) and the role of investigation and laboratory tests: An expert consensus report
title_full_unstemmed The diagnosis of fungal neglected tropical diseases (fungal NTDs) and the role of investigation and laboratory tests: An expert consensus report
title_sort The diagnosis of fungal neglected tropical diseases (fungal NTDs) and the role of investigation and laboratory tests: An expert consensus report
dc.subject.keyword.spa.fl_str_mv Impression material
Article
Biopsy
Chromomycosis
Consensus
Fungus culture
Health care utilization
Histopathology
Human
Laboratory diagnosis
Laboratory test
Microscopy
Mycetoma
Mycosis
Neglected disease
Nonhuman
Patient referral
Point of care testing
Sporotrichosis
Tropical disease
Chromoblastomycosis
Fungal ntds
Integrated approaches
Laboratory diagnosis
Mycetoma
Sporotrichosis
topic Impression material
Article
Biopsy
Chromomycosis
Consensus
Fungus culture
Health care utilization
Histopathology
Human
Laboratory diagnosis
Laboratory test
Microscopy
Mycetoma
Mycosis
Neglected disease
Nonhuman
Patient referral
Point of care testing
Sporotrichosis
Tropical disease
Chromoblastomycosis
Fungal ntds
Integrated approaches
Laboratory diagnosis
Mycetoma
Sporotrichosis
description The diagnosis of fungal Neglected Tropical Diseases (NTD) is primarily based on initial visual recognition of a suspected case followed by confirmatory laboratory testing, which is often limited to specialized facilities. Although molecular and serodiagnostic tools have advanced, a substantial gap remains between the desirable and the practical in endemic settings. To explore this issue further, we conducted a survey of subject matter experts on the optimal diagnostic methods sufficient to initiate treatment in well-equipped versus basic healthcare settings, as well as optimal sampling methods, for three fungal NTDs: mycetoma, chromoblastomycosis, and sporotrichosis. A survey of 23 centres found consensus on the key role of semi-invasive sampling methods such as biopsy diagnosis as compared with swabs or impression smears, and on the importance of histopathology, direct microscopy, and culture for mycetoma and chromoblastomycosis confirmation in well-equipped laboratories. In basic healthcare settings, direct microscopy combined with clinical signs were reported to be the most useful diagnostic indicators to prompt referral for treatment. The survey identified that the diagnosis of sporotrichosis is the most problematic with poor sensitivity across the most widely available laboratory tests except fungal culture, highlighting the need to improve mycological diagnostic capacity and to develop innovative diagnostic solutions. Fungal microscopy and culture are now recognized as WHO essential diagnostic tests and better training in their application will help improve the situation. For mycetoma and sporotrichosis, in particular, advances in identifying specific marker antigens or genomic sequences may pave the way for new laboratory-based or point-of-care tests, although this is a formidable task given the large number of different organisms that can cause fungal NTDs. © 2019 by the authors.
publishDate 2019
dc.date.created.spa.fl_str_mv 2019
dc.date.accessioned.none.fl_str_mv 2020-05-26T00:01:42Z
dc.date.available.none.fl_str_mv 2020-05-26T00:01:42Z
dc.type.eng.fl_str_mv article
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dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.3390/tropicalmed4040122
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/23397
url https://doi.org/10.3390/tropicalmed4040122
https://repository.urosario.edu.co/handle/10336/23397
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationIssue.none.fl_str_mv No. 4
dc.relation.citationTitle.none.fl_str_mv Tropical Medicine and Infectious Disease
dc.relation.citationVolume.none.fl_str_mv Vol. 4
dc.relation.ispartof.spa.fl_str_mv Tropical Medicine and Infectious Disease, Vol.4, No.4 (2019)
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spelling 25a7066e-8c09-4c4a-b302-4102ecb2bd7f-11a64ea7f-fb33-4fb3-9ba9-c7d9c276e4c0-1560d0acd-cfde-491e-8828-706fc98ef1dd-10bbd4983-1013-4dce-9267-5538746a74af-1f716a5ea-7d2e-4f78-ba54-e75d1b309d12-129ffb56f-be8d-455e-86d8-d20dcc8121c1-1b9cd9d60-59d6-4b7e-8b2d-c07020051573-1254403b3-919c-4e75-bd88-b7a907d9fc64-1c17e7749-9fca-4197-88da-13268e1275c8-14b4f2196-508b-44d9-8683-1a84cb7e7a78-121774735-3d29-4a64-a9b8-c85f9547878e-1be1f7e98-f639-4e15-9b8e-5659148ddc55-1fec3876d-4df4-4efc-9bca-05e787b4a171-14ce0e8a3-32d8-48c5-b27e-5eac38c86ac4-1538fcbe0-b96c-4cea-8e57-57cf8c2be157-159f2cb96-3787-452a-adaf-bb4deb180a3b-138501a47-61c7-49e7-b1ab-db627dc7e26f-1baa92ab0-68c4-464e-aa4b-e222a94bc841-105e4b014-5c7f-4151-9c5f-ae28873bca9e-15496f64f-a07d-46a5-8bee-043a5bcbeebd-1b950cdc4-6b9f-4101-a12d-6dcf3e8665fa-1430461946002020-05-26T00:01:42Z2020-05-26T00:01:42Z2019The diagnosis of fungal Neglected Tropical Diseases (NTD) is primarily based on initial visual recognition of a suspected case followed by confirmatory laboratory testing, which is often limited to specialized facilities. Although molecular and serodiagnostic tools have advanced, a substantial gap remains between the desirable and the practical in endemic settings. To explore this issue further, we conducted a survey of subject matter experts on the optimal diagnostic methods sufficient to initiate treatment in well-equipped versus basic healthcare settings, as well as optimal sampling methods, for three fungal NTDs: mycetoma, chromoblastomycosis, and sporotrichosis. A survey of 23 centres found consensus on the key role of semi-invasive sampling methods such as biopsy diagnosis as compared with swabs or impression smears, and on the importance of histopathology, direct microscopy, and culture for mycetoma and chromoblastomycosis confirmation in well-equipped laboratories. In basic healthcare settings, direct microscopy combined with clinical signs were reported to be the most useful diagnostic indicators to prompt referral for treatment. The survey identified that the diagnosis of sporotrichosis is the most problematic with poor sensitivity across the most widely available laboratory tests except fungal culture, highlighting the need to improve mycological diagnostic capacity and to develop innovative diagnostic solutions. Fungal microscopy and culture are now recognized as WHO essential diagnostic tests and better training in their application will help improve the situation. For mycetoma and sporotrichosis, in particular, advances in identifying specific marker antigens or genomic sequences may pave the way for new laboratory-based or point-of-care tests, although this is a formidable task given the large number of different organisms that can cause fungal NTDs. © 2019 by the authors.application/pdfhttps://doi.org/10.3390/tropicalmed4040122https://repository.urosario.edu.co/handle/10336/23397engMDPI AGNo. 4Tropical Medicine and Infectious DiseaseVol. 4Tropical Medicine and Infectious Disease, Vol.4, No.4 (2019)https://www.scopus.com/inward/record.uri?eid=2-s2.0-85073010116&doi=10.3390%2ftropicalmed4040122&partnerID=40&md5=2fc4f786ab07dab130cf2a45e326c304Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURImpression materialArticleBiopsyChromomycosisConsensusFungus cultureHealth care utilizationHistopathologyHumanLaboratory diagnosisLaboratory testMicroscopyMycetomaMycosisNeglected diseaseNonhumanPatient referralPoint of care testingSporotrichosisTropical diseaseChromoblastomycosisFungal ntdsIntegrated approachesLaboratory diagnosisMycetomaSporotrichosisThe diagnosis of fungal neglected tropical diseases (fungal NTDs) and the role of investigation and laboratory tests: An expert consensus reportarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Hay, RoderickDenning, David WBonifaz, AlexandroQueiroz-Telles, FlavioBeer, KarlynBustamante, BeatrizChakrabarti, Arunalokede Guadalupe Chavez-Lopez, MariaChiller, TomCornet, MurielEstrada, RobertoEstrada-Chavez, GuadalupeFahal, AhmedLi, RuoyuMahabeer, YesholataMosam, AnisaRamarozatovo, Lala SoavinaAndrianarivelo, Mala RakotoRabenja, Fahafahantsoa Rapelanorovan de Sande, WendyZijlstra, Eduard EGómez Giraldo, Beatríz LucíaORIGINALtropicalmed-04-00122.pdfapplication/pdf2077265https://repository.urosario.edu.co/bitstreams/b5f66f6d-63aa-4ecc-93bb-8d73533395bf/download445b3d0c6e5f6d555b31db9bb63610b2MD51TEXTtropicalmed-04-00122.pdf.txttropicalmed-04-00122.pdf.txtExtracted texttext/plain46605https://repository.urosario.edu.co/bitstreams/4eb5d5cf-87c0-47e4-a725-f3dbbbfa664f/downloada90c94a174d395f8d3823ad9f5b80fceMD52THUMBNAILtropicalmed-04-00122.pdf.jpgtropicalmed-04-00122.pdf.jpgGenerated Thumbnailimage/jpeg4955https://repository.urosario.edu.co/bitstreams/1f9fc2dd-daaf-4e88-9ca5-2eb431be3998/download295bb2c060a1bf46f4fe4cbea871583fMD5310336/23397oai:repository.urosario.edu.co:10336/233972022-05-02 07:37:20.952485https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co