Genomic survey of pathogenicity determinants and VNTR markers in the cassava bacterial pathogen Xanthomonas axonopodis pv. manihotis strain CIO151

l pasado 21 de abril de 2009 la Honorable Consiliatura dio su aprobación al proyecto denominado Escuela de Medicina y Ciencias de la Salud

Autores:

Tipo de recurso:

Fecha de publicación:: 2013

Institución:: Universidad del Rosario

Repositorio:: Repositorio EdocUR - U. Rosario

Idioma:: eng

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dc.title.spa.fl_str_mv	Genomic survey of pathogenicity determinants and VNTR markers in the cassava bacterial pathogen Xanthomonas axonopodis pv. manihotis strain CIO151
dc.title.TranslatedTitle.spa.fl_str_mv	Estudio genómico de los determinantes de patogenicidad y marcadores VNTR en el patógeno bacteriano de la yuca Xanthomonas axonopodis pv. Manihotis Strain CIO151
title	Genomic survey of pathogenicity determinants and VNTR markers in the cassava bacterial pathogen Xanthomonas axonopodis pv. manihotis strain CIO151
spellingShingle	Genomic survey of pathogenicity determinants and VNTR markers in the cassava bacterial pathogen Xanthomonas axonopodis pv. manihotis strain CIO151 Determinantes de patogenicidad Marcadores VNTR Xanthomonas axonopodis pv. Manihotis Strain CIO151 Xanthomonas axonopodis pv. manihotis (Xam) Xam strain CIO151
title_short	Genomic survey of pathogenicity determinants and VNTR markers in the cassava bacterial pathogen Xanthomonas axonopodis pv. manihotis strain CIO151
title_full	Genomic survey of pathogenicity determinants and VNTR markers in the cassava bacterial pathogen Xanthomonas axonopodis pv. manihotis strain CIO151
title_fullStr	Genomic survey of pathogenicity determinants and VNTR markers in the cassava bacterial pathogen Xanthomonas axonopodis pv. manihotis strain CIO151
title_full_unstemmed	Genomic survey of pathogenicity determinants and VNTR markers in the cassava bacterial pathogen Xanthomonas axonopodis pv. manihotis strain CIO151
title_sort	Genomic survey of pathogenicity determinants and VNTR markers in the cassava bacterial pathogen Xanthomonas axonopodis pv. manihotis strain CIO151
dc.subject.spa.fl_str_mv	Determinantes de patogenicidad Marcadores VNTR Xanthomonas axonopodis pv. Manihotis Strain CIO151
topic	Determinantes de patogenicidad Marcadores VNTR Xanthomonas axonopodis pv. Manihotis Strain CIO151 Xanthomonas axonopodis pv. manihotis (Xam) Xam strain CIO151
dc.subject.keyword.spa.fl_str_mv	Xanthomonas axonopodis pv. manihotis (Xam) Xam strain CIO151
description	l pasado 21 de abril de 2009 la Honorable Consiliatura dio su aprobación al proyecto denominado Escuela de Medicina y Ciencias de la Salud
publishDate	2013
dc.date.created.spa.fl_str_mv	2013-11-22
dc.date.accessioned.none.fl_str_mv	2020-08-19T14:43:49Z
dc.date.available.none.fl_str_mv	2020-08-19T14:43:49Z
dc.type.eng.fl_str_mv	article
dc.type.coarversion.fl_str_mv	http://purl.org/coar/version/c_970fb48d4fbd8a85
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dc.type.spa.spa.fl_str_mv	Artículo
dc.identifier.doi.none.fl_str_mv	https://doi.org/10.1371/journal.pone.0079704
dc.identifier.issn.none.fl_str_mv	ISSN: 1549-1277
dc.identifier.uri.none.fl_str_mv	https://repository.urosario.edu.co/handle/10336/27776
url	https://doi.org/10.1371/journal.pone.0079704 https://repository.urosario.edu.co/handle/10336/27776
identifier_str_mv	ISSN: 1549-1277
dc.language.iso.spa.fl_str_mv	eng
language	eng
dc.relation.citationEndPage.none.fl_str_mv	18
dc.relation.citationIssue.none.fl_str_mv	No. 11
dc.relation.citationStartPage.none.fl_str_mv	1
dc.relation.citationTitle.none.fl_str_mv	PLoS One
dc.relation.citationVolume.none.fl_str_mv	Vol. 8
dc.relation.ispartof.spa.fl_str_mv	PlosOne, ISSN:1549-1277, Vol.8, No.11( Noviembre 2013);pp.1-18
dc.relation.uri.spa.fl_str_mv	https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0079704&type=printable
dc.rights.coar.fl_str_mv	http://purl.org/coar/access_right/c_abf2
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rights_invalid_str_mv	Abierto (Texto Completo) http://purl.org/coar/access_right/c_abf2
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dc.publisher.spa.fl_str_mv	Plos
dc.source.spa.fl_str_mv	PLoS One
institution	Universidad del Rosario
dc.source.instname.none.fl_str_mv	instname:Universidad del Rosario
dc.source.reponame.none.fl_str_mv	reponame:Repositorio Institucional EdocUR
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spelling	2a122d8b-d724-4066-83bd-ed0ab630fb35-122a7e01f-fc8b-469f-8b94-0dbbd6e178df-18aafd342-a120-456d-b48b-f1712518b412-16d30096f-8a95-40a2-8519-5aeeef0845a9-1c2a0dd59-b703-4131-8c69-dbc9f36f803f-185580663-e7ee-4732-9e54-29c47b4f521d-1a3abe484-03cc-47fc-8b5d-8ef3a4e7762b-104baae22-9c37-4dff-868b-a78cae452cce-1531073116002020-08-19T14:43:49Z2020-08-19T14:43:49Z2013-11-22l pasado 21 de abril de 2009 la Honorable Consiliatura dio su aprobación al proyecto denominado Escuela de Medicina y Ciencias de la SaludXanthomonas axonopodis pv. manihotis (Xam) is the causal agent of bacterial blight of cassava, which is among the main components of human diet in Africa and South America. Current information about the molecular pathogenicity factors involved in the infection process of this organism is limited. Previous studies in other bacteria in this genus suggest that advanced draft genome sequences are valuable resources for molecular studies on their interaction with plants and could provide valuable tools for diagnostics and detection. Here we have generated the first manually annotated high-quality draft genome sequence of Xam strain CIO151. Its genomic structure is similar to that of other xanthomonads, especially Xanthomonas euvesicatoria and Xanthomonas citri pv. citri species. Several putative pathogenicity factors were identified, including type III effectors, cell wall-degrading enzymes and clusters encoding protein secretion systems. Specific characteristics in this genome include changes in the xanthomonadin cluster that could explain the lack of typical yellow color in all strains of this pathovar and the presence of 50 regions in the genome with atypical nucleotide composition. The genome sequence was used to predict and evaluate 22 variable number of tandem repeat (VNTR) loci that were subsequently demonstrated as polymorphic in representative Xam strains. Our results demonstrate that Xanthomonas axonopodis pv. manihotis strain CIO151 possesses ten clusters of pathogenicity factors conserved within the genus Xanthomonas. We report 126 genes that are potentially unique to Xam, as well as potential horizontal transfer events in the history of the genome. The relation of these regions with virulence and pathogenicity could explain several aspects of the biology of this pathogen, including its ability to colonize both vascular and non-vascular tissues of cassava plants. A set of 16 robust, polymorphic VNTR loci will be useful to develop a multi-locus VNTR analysis scheme for epidemiological surveillance of this disease.application/pdfhttps://doi.org/10.1371/journal.pone.0079704ISSN: 1549-1277https://repository.urosario.edu.co/handle/10336/27776engPlos18No. 111PLoS OneVol. 8PlosOne, ISSN:1549-1277, Vol.8, No.11( Noviembre 2013);pp.1-18https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0079704&type=printableAbierto (Texto Completo)http://purl.org/coar/access_right/c_abf2PLoS Oneinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURDeterminantes de patogenicidadMarcadores VNTRXanthomonas axonopodis pv. Manihotis Strain CIO151Xanthomonas axonopodis pv. manihotis (Xam)Xam strain CIO151Genomic survey of pathogenicity determinants and VNTR markers in the cassava bacterial pathogen Xanthomonas axonopodis pv. manihotis strain CIO151Estudio genómico de los determinantes de patogenicidad y marcadores VNTR en el patógeno bacteriano de la yuca Xanthomonas axonopodis pv. Manihotis Strain CIO151articleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Arrieta-Ortiz, Mario L.Rodríguez-R, Luis M.Pérez-Quintero, Álvaro L.Poulin, LucieDíaz, Ana C.Arias Rojas, NathaliaTrujillo, CesarRestrepo Benavides, MarianaPardo Díaz, Geimy CarolinaORIGINALjournal-pone-0079704.pdfapplication/pdf1541707https://repository.urosario.edu.co/bitstreams/7acc9b75-e48b-4ab5-b49f-1a6a221fa484/download5dc9a0370fcd130cd00701fcf29e3f57MD51TEXTjournal-pone-0079704.pdf.txtjournal-pone-0079704.pdf.txtExtracted texttext/plain100692https://repository.urosario.edu.co/bitstreams/07938b2a-a6a4-44ea-a0ca-92a71f6fa1ba/download1a6fc5109dd1c9be7a636c7db2265f81MD52THUMBNAILjournal-pone-0079704.pdf.jpgjournal-pone-0079704.pdf.jpgGenerated Thumbnailimage/jpeg5276https://repository.urosario.edu.co/bitstreams/7112313f-a5d3-4569-a181-3bb8249de622/downloada54fb33a7b94fed7b798aee7cbea4c5dMD5310336/27776oai:repository.urosario.edu.co:10336/277762021-06-03 00:50:58.721https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co

Genomic survey of pathogenicity determinants and VNTR markers in the cassava bacterial pathogen Xanthomonas axonopodis pv. manihotis strain CIO151

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