Identification and evaluation of universal epitopes in Plasmodium vivax Duffy binding protein

Selected PvDBP-derived synthetic peptides were tested in competition assays with HLA molecules in order to identify and evaluate their binding to a wide range of MHC class II molecules. Binding was evaluated as the peptide's ability to displace the biotinylated control peptide (HA306-318) and was detected by a conventional ELISA. Thus, one epitope for the HLA-DR1 molecule, two epitopes for the HLA-DR4 molecule, six epitopes for the HLA-DR7 molecule and three epitopes for the HLA-DR11 molecule displaying a high binding percentage (above 50%) were experimentally obtained. The in vitro results were compared with the epitope prediction results. Two peptides behaved as universal epitopes since they bound to a larger number of HLA-DR molecules. Given that these peptides are located in the conserved PvDBP region II, they could be considered good candidates to be included in the design of a synthetic vaccine against Plasmodium vivax malaria. © 2008 Elsevier Inc. All rights reserved.

• Fecha de publicación: 2008
• Institución: Universidad del Rosario
• Repositorio: Repositorio EdocUR - U. Rosario
• Idioma: eng
• OAI Identifier: oai:repository.urosario.edu.co:10336/23504
• Acceso en línea: https://doi.org/10.1016/j.bbrc.2008.10.153; https://repository.urosario.edu.co/handle/10336/23504
• Palabra clave: Duffy binding protein; Epitope; Hla dr1 antigen; Hla dr11 antigen; Hla dr4 antigen; Hla dr7 antigen; Unclassified drug; Antigen binding; Article; Biotinylation; Controlled study; Enzyme linked immunosorbent assay; Nucleotide sequence; Plasmodium vivax; Priority journal; Protein analysis; Amino acid sequence; Animals; Epitope mapping; Hla-dr antigens; Immunodominant epitopes; Malaria vaccines; Molecular sequence data; Peptides; Plasmodium vivax; Protein conformation; Protozoan proteins; Receptors, cell surface; Sequence alignment; Vaccines, synthetic; Plasmodium vivax; Duffy binding protein (dbp); Malaria; Plasmodium vivax; Universal epitopes; cell surface; protozoan; synthetic
• License: Abierto (Texto Completo)