Slight temperature changes cause rapid transcriptomic responses in Trypanosoma cruzi metacyclic trypomastigotes.

BACKGROUND: Severe changes in temperature can affect the behavior and ecology of some infectious agents. Trypanosoma cruzi is a protozoan that causes Chagas disease. This parasite has high genetic variability and can be divided into six discrete typing units (DTUs). Trypanosoma cruzi also has a comp...

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Tipo de recurso:
Fecha de publicación:
2020
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/25027
Acceso en línea:
https://doi.org/10.1186/s13071-020-04125-y
https://repository.urosario.edu.co/handle/10336/25027
Palabra clave:
DTUs
Metacyclic trypomastigotes
RNAseq
Temperature
Transcriptomic
Trypanosoma cruzi
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License
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id EDOCUR2_3bc81c63055b7fb83a1a83661f44b2c9
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spelling 3a39d4a1-8a8d-431d-951a-63c54f374456-15f0bfa81-3587-4e7d-9a77-20bc03da8208-1b8f144ac-9415-4272-8914-26a08b37dfef-1fa0d2708-a3d5-4e7f-a200-9e4565bf39ef-1fe062efa-2c45-40ad-9076-805a8d1e00ac-110117161186002020-06-11T13:22:07Z2020-06-11T13:22:07Z2020BACKGROUND: Severe changes in temperature can affect the behavior and ecology of some infectious agents. Trypanosoma cruzi is a protozoan that causes Chagas disease. This parasite has high genetic variability and can be divided into six discrete typing units (DTUs). Trypanosoma cruzi also has a complex life-cycle, which includes the process of metacyclogenesis when non-infective epimastigote forms are differentiated into infective metacyclic trypomastigotes (MT). Studies in triatomines have shown that changes in temperature also affect the number and viability of MT.METHODS: The objective of this study was to evaluate how temperature affects the transcriptional profiles of T. cruzi I and II (TcI and TcII) MT by exposing parasites to two temperatures (27°C and 28°C) and comparing those to normal culture conditions at 26°C. Subsequently, RNA-seq was conducted and differentially expressed genes were quantified and associated to metabolic pathways.RESULTS: A statistically significant difference was observed in the number of MT between the temperatures evaluated and the control, TcII DTU was not strongly affected to exposure to high temperatures compared to TcI. Similar results were found when we analyzed gene expression in this DTU, with the greatest number of differentially expressed genes being observed at 28°C, which could indicate a dysregulation of different signaling pathways under this temperature. Chromosome analysis indicated that chromosome 1 harbored the highest number of changes for both DTUs for all thermal treatments. Finally, gene ontology (GO) analyses showed a decrease in the coding RNAs involved in the regulation of processes related to the metabolism of lipids and carbohydrates, the evasion of oxidative stress, and proteolysis and phosphorylation processes, and a decrease in RNAs coding to ribosomal proteins in TcI and TcII, along with an increase in the expression of surface metalloprotease GP63 in TcII.CONCLUSIONS: Slight temperature shifts lead to increased cell death of metacyclic trypomastigotes because of the deregulation of gene expression of different processes essential for the TcI and TcII DTUs of T. cruzi.application/pdfhttps://doi.org/10.1186/s13071-020-04125-y1756-3305https://repository.urosario.edu.co/handle/10336/25027engBMC255No. 1255Parasites & vectorsVol. 13Parasites & vectors, ISSN:1756-3305, Vol.13, No.1 (2020); pp. 255-255Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURDTUsMetacyclic trypomastigotesRNAseqTemperatureTranscriptomicTrypanosoma cruziSlight temperature changes cause rapid transcriptomic responses in Trypanosoma cruzi metacyclic trypomastigotes.articleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Cruz-Saavedra, LissaMunoz, MarinaPatino, Luz HelenaVallejo, Gustavo AGuhl, FelipeRamírez, Juan DavidORIGINALs13071-020-04125-y.pdfapplication/pdf1916833https://repository.urosario.edu.co/bitstreams/7891af47-4407-4da8-aa7c-3b04177eeda7/download889ff7593c5e7b179e3246cf0e40bd73MD51TEXTs13071-020-04125-y.pdf.txts13071-020-04125-y.pdf.txtExtracted texttext/plain78683https://repository.urosario.edu.co/bitstreams/6d356694-5018-4e4a-aa0c-fed6b52d9c48/download51adc133a60f9cc4afd2384b31ab084fMD52THUMBNAILs13071-020-04125-y.pdf.jpgs13071-020-04125-y.pdf.jpgGenerated Thumbnailimage/jpeg4527https://repository.urosario.edu.co/bitstreams/dce29ebe-a441-47e7-a758-467e61834232/download5a699671e0c0b784e3c011111986a749MD5310336/25027oai:repository.urosario.edu.co:10336/250272021-06-03 00:50:52.669https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Slight temperature changes cause rapid transcriptomic responses in Trypanosoma cruzi metacyclic trypomastigotes.
title Slight temperature changes cause rapid transcriptomic responses in Trypanosoma cruzi metacyclic trypomastigotes.
spellingShingle Slight temperature changes cause rapid transcriptomic responses in Trypanosoma cruzi metacyclic trypomastigotes.
DTUs
Metacyclic trypomastigotes
RNAseq
Temperature
Transcriptomic
Trypanosoma cruzi
title_short Slight temperature changes cause rapid transcriptomic responses in Trypanosoma cruzi metacyclic trypomastigotes.
title_full Slight temperature changes cause rapid transcriptomic responses in Trypanosoma cruzi metacyclic trypomastigotes.
title_fullStr Slight temperature changes cause rapid transcriptomic responses in Trypanosoma cruzi metacyclic trypomastigotes.
title_full_unstemmed Slight temperature changes cause rapid transcriptomic responses in Trypanosoma cruzi metacyclic trypomastigotes.
title_sort Slight temperature changes cause rapid transcriptomic responses in Trypanosoma cruzi metacyclic trypomastigotes.
dc.subject.keyword.spa.fl_str_mv DTUs
Metacyclic trypomastigotes
RNAseq
Temperature
Transcriptomic
Trypanosoma cruzi
topic DTUs
Metacyclic trypomastigotes
RNAseq
Temperature
Transcriptomic
Trypanosoma cruzi
description BACKGROUND: Severe changes in temperature can affect the behavior and ecology of some infectious agents. Trypanosoma cruzi is a protozoan that causes Chagas disease. This parasite has high genetic variability and can be divided into six discrete typing units (DTUs). Trypanosoma cruzi also has a complex life-cycle, which includes the process of metacyclogenesis when non-infective epimastigote forms are differentiated into infective metacyclic trypomastigotes (MT). Studies in triatomines have shown that changes in temperature also affect the number and viability of MT.METHODS: The objective of this study was to evaluate how temperature affects the transcriptional profiles of T. cruzi I and II (TcI and TcII) MT by exposing parasites to two temperatures (27°C and 28°C) and comparing those to normal culture conditions at 26°C. Subsequently, RNA-seq was conducted and differentially expressed genes were quantified and associated to metabolic pathways.RESULTS: A statistically significant difference was observed in the number of MT between the temperatures evaluated and the control, TcII DTU was not strongly affected to exposure to high temperatures compared to TcI. Similar results were found when we analyzed gene expression in this DTU, with the greatest number of differentially expressed genes being observed at 28°C, which could indicate a dysregulation of different signaling pathways under this temperature. Chromosome analysis indicated that chromosome 1 harbored the highest number of changes for both DTUs for all thermal treatments. Finally, gene ontology (GO) analyses showed a decrease in the coding RNAs involved in the regulation of processes related to the metabolism of lipids and carbohydrates, the evasion of oxidative stress, and proteolysis and phosphorylation processes, and a decrease in RNAs coding to ribosomal proteins in TcI and TcII, along with an increase in the expression of surface metalloprotease GP63 in TcII.CONCLUSIONS: Slight temperature shifts lead to increased cell death of metacyclic trypomastigotes because of the deregulation of gene expression of different processes essential for the TcI and TcII DTUs of T. cruzi.
publishDate 2020
dc.date.accessioned.none.fl_str_mv 2020-06-11T13:22:07Z
dc.date.available.none.fl_str_mv 2020-06-11T13:22:07Z
dc.date.created.spa.fl_str_mv 2020
dc.type.eng.fl_str_mv article
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dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1186/s13071-020-04125-y
dc.identifier.issn.none.fl_str_mv 1756-3305
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/25027
url https://doi.org/10.1186/s13071-020-04125-y
https://repository.urosario.edu.co/handle/10336/25027
identifier_str_mv 1756-3305
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 255
dc.relation.citationIssue.none.fl_str_mv No. 1
dc.relation.citationStartPage.none.fl_str_mv 255
dc.relation.citationTitle.none.fl_str_mv Parasites & vectors
dc.relation.citationVolume.none.fl_str_mv Vol. 13
dc.relation.ispartof.spa.fl_str_mv Parasites & vectors, ISSN:1756-3305, Vol.13, No.1 (2020); pp. 255-255
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rights_invalid_str_mv Abierto (Texto Completo)
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