Toxoplasma gondii: Immunogenicity and protection by P30 peptides in a murine model

Vaccines are promising for the control of toxoplasmosis. Here, we evaluated the immunogenicity of 17 peptides derived from SAG1 surface protein of Toxoplasma gondii in CH3 mice. Only 8 of 16 peptides induced specific antibodies. After a lethal challenge, only the vaccination with 4 of 17 peptides th...

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Autores:
Tipo de recurso:
Fecha de publicación:
2006
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/24037
Acceso en línea:
https://doi.org/10.1016/j.exppara.2006.02.005
https://repository.urosario.edu.co/handle/10336/24037
Palabra clave:
Cysteinylisoleucyllysylglycylaspartylaspartylalanylglutaminylseryl cysteinylmethionylvalylthreonylvalylthreonylvalylglutaminylalanylarginylalanine
Isoleucylcysteinylprolylalanylglycylthreonylthreonylserylserylcysteinyl threonylseryllysylalanylvalylthreonylleucylserylserylleucine
Isoleucyllysylleucylthreonylvalylprolylisoleucylglutamyllysylphenylalanyl prolylvalylthreonylthreonylglutaminylthreonylphenylalanylvalylvalylglycine
Isoleucylprolylglutamylalanylglutamylaspartylseryltryptophanyltryptophanyl threonylglycylaspartylserylalanylserylleucylaspartylthreonylalanylglycine
Leucylarginylcysteinylglycylvalylmethionylalanylserylaspartylprolylprolyl leucylvalylalanylasparaginylglutaminylvalylvalylthreonylcysteine
Lysylalanylvalylarginylarginylalanylvalylthreonylalanylglycylvalyl phenylalanylalanylalanylprolylthreonylleucylmethionylserylphenylalanine
Lysylcysteinylprolyllysylthreonylalanylleucylthreonylglutamylprolylprolyl threonylleucylalanyltyrosylserylprolylasparaginylarginylglutamine
Lysylserylphenylalanyllysylaspartylisoleucylleucylprolyllysylleucyl threonylglutamylasparaginylprolyltryptophanylglutaminylglycylasparaginylalanyl serine
Lysylvalylprolylglutaminylaspartylasparaginylasparaginylglutaminyltyrosyl cysteinylserylglycylthreonylthreonylleucylthreonylglycylcysteinylasparaginyl glutamic acid
Membrane protein
Methionylserylvalylserylleucylhistidylhistidylphenylalanylisoleucyl isoleucylserylserylglycylphenylalanylleucylthreonylserylmethionylphenylalanyl proline
Peptide derivative
Phenylalanylalanylglycylalanylalanylglycylserylalanyllysylserylalanyl alanylglycylthreonylalanylserylhistidylvalylserylisoleucine
Phenylalanylalanylmethionylvalylisoleucylglycylleucylisoleucylglycylseryl isoleucylalanylalanylcysteinylvalylalanine
Prolylaspartyllysyllysylserylthreonylalanylalanylvalylisoleucylleucyl threonylprolylthreonylglutamylasparaginylhistidylphenylalanylthreonylleucine
Protein sag1
Serylaspartyllysylglycylalanylthreonylleucylthreonylisoleucyllysyllysyl glutamylalanylphenylalanylprolylalanylglutamylseryllysylserine
Serylserylvalylvalylasparaginylasparaginylalanylarginylcysteinylseryl tyrosylglycylalanylaspartylserylthreonylleucylglycylproline
Unclassified drug
Valylisoleucylisoleucylglycylcysteinylthreonylglycylglycylserylprolyl glutamyllysylhistidylhistidylcysteinylthreonylvalyllysylleucylglutamic acid
Valyllysylleucylserylalanylglutamylglycylprolylthreonylthreonylmethionyl threonylleucylvalylcysteinylglycyllysylaspartylglycylvaline
Animal experiment
Animal model
Article
Carboxy terminal sequence
Controlled study
Immunogenicity
Male
Mouse
Nonhuman
Nucleotide sequence
Priority journal
Survival
Toxoplasma gondii
Toxoplasmosis
Vaccination
Amino acid sequence
Animals
Male
Mice
Molecular sequence data
Peptides
Protozoan proteins
Protozoan vaccines
Toxoplasma
Murinae
Toxoplasma
Toxoplasma gondii
Murine model
P30
Peptides
Sag1
Toxoplasma gondii
Vaccines
animal
surface
secondary
protozoan
protozoan
inbred c3h
Antibodies
Antigens
Antigens
Immunization
Mice
Toxoplasmosis
Rights
License
Abierto (Texto Completo)
Description
Summary:Vaccines are promising for the control of toxoplasmosis. Here, we evaluated the immunogenicity of 17 peptides derived from SAG1 surface protein of Toxoplasma gondii in CH3 mice. Only 8 of 16 peptides induced specific antibodies. After a lethal challenge, only the vaccination with 4 of 17 peptides that were from the carboxy terminal end of the protein conferred significant survival. Our work shows that vaccination with peptides from the carboxy-terminal positions of SAG1 major surface protein of Toxoplasma protects mice against a lethal challenge. © 2006 Elsevier Inc. All rights reserved.