Toxoplasma gondii: Immunogenicity and protection by P30 peptides in a murine model

Vaccines are promising for the control of toxoplasmosis. Here, we evaluated the immunogenicity of 17 peptides derived from SAG1 surface protein of Toxoplasma gondii in CH3 mice. Only 8 of 16 peptides induced specific antibodies. After a lethal challenge, only the vaccination with 4 of 17 peptides that were from the carboxy terminal end of the protein conferred significant survival. Our work shows that vaccination with peptides from the carboxy-terminal positions of SAG1 major surface protein of Toxoplasma protects mice against a lethal challenge. © 2006 Elsevier Inc. All rights reserved.

• Fecha de publicación: 2006
• Institución: Universidad del Rosario
• Repositorio: Repositorio EdocUR - U. Rosario
• Idioma: eng
• OAI Identifier: oai:repository.urosario.edu.co:10336/24037
• Acceso en línea: https://doi.org/10.1016/j.exppara.2006.02.005; https://repository.urosario.edu.co/handle/10336/24037
• Palabra clave: Cysteinylisoleucyllysylglycylaspartylaspartylalanylglutaminylseryl cysteinylmethionylvalylthreonylvalylthreonylvalylglutaminylalanylarginylalanine; Isoleucylcysteinylprolylalanylglycylthreonylthreonylserylserylcysteinyl threonylseryllysylalanylvalylthreonylleucylserylserylleucine; Isoleucyllysylleucylthreonylvalylprolylisoleucylglutamyllysylphenylalanyl prolylvalylthreonylthreonylglutaminylthreonylphenylalanylvalylvalylglycine; Isoleucylprolylglutamylalanylglutamylaspartylseryltryptophanyltryptophanyl threonylglycylaspartylserylalanylserylleucylaspartylthreonylalanylglycine; Leucylarginylcysteinylglycylvalylmethionylalanylserylaspartylprolylprolyl leucylvalylalanylasparaginylglutaminylvalylvalylthreonylcysteine; Lysylalanylvalylarginylarginylalanylvalylthreonylalanylglycylvalyl phenylalanylalanylalanylprolylthreonylleucylmethionylserylphenylalanine; Lysylcysteinylprolyllysylthreonylalanylleucylthreonylglutamylprolylprolyl threonylleucylalanyltyrosylserylprolylasparaginylarginylglutamine; Lysylserylphenylalanyllysylaspartylisoleucylleucylprolyllysylleucyl threonylglutamylasparaginylprolyltryptophanylglutaminylglycylasparaginylalanyl serine; Lysylvalylprolylglutaminylaspartylasparaginylasparaginylglutaminyltyrosyl cysteinylserylglycylthreonylthreonylleucylthreonylglycylcysteinylasparaginyl glutamic acid; Membrane protein; Methionylserylvalylserylleucylhistidylhistidylphenylalanylisoleucyl isoleucylserylserylglycylphenylalanylleucylthreonylserylmethionylphenylalanyl proline; Peptide derivative; Phenylalanylalanylglycylalanylalanylglycylserylalanyllysylserylalanyl alanylglycylthreonylalanylserylhistidylvalylserylisoleucine; Phenylalanylalanylmethionylvalylisoleucylglycylleucylisoleucylglycylseryl isoleucylalanylalanylcysteinylvalylalanine; Prolylaspartyllysyllysylserylthreonylalanylalanylvalylisoleucylleucyl threonylprolylthreonylglutamylasparaginylhistidylphenylalanylthreonylleucine; Protein sag1; Serylaspartyllysylglycylalanylthreonylleucylthreonylisoleucyllysyllysyl glutamylalanylphenylalanylprolylalanylglutamylseryllysylserine; Serylserylvalylvalylasparaginylasparaginylalanylarginylcysteinylseryl tyrosylglycylalanylaspartylserylthreonylleucylglycylproline; Unclassified drug; Valylisoleucylisoleucylglycylcysteinylthreonylglycylglycylserylprolyl glutamyllysylhistidylhistidylcysteinylthreonylvalyllysylleucylglutamic acid; Valyllysylleucylserylalanylglutamylglycylprolylthreonylthreonylmethionyl threonylleucylvalylcysteinylglycyllysylaspartylglycylvaline; Animal experiment; Animal model; Article; Carboxy terminal sequence; Controlled study; Immunogenicity; Male; Mouse; Nonhuman; Nucleotide sequence; Priority journal; Survival; Toxoplasma gondii; Toxoplasmosis; Vaccination; Amino acid sequence; Animals; Male; Mice; Molecular sequence data; Peptides; Protozoan proteins; Protozoan vaccines; Toxoplasma; Murinae; Toxoplasma; Toxoplasma gondii; Murine model; P30; Peptides; Sag1; Toxoplasma gondii; Vaccines; animal; surface; secondary; protozoan; protozoan; inbred c3h; Antibodies; Antigens; Antigens; Immunization; Mice; Toxoplasmosis
• License: Abierto (Texto Completo)