A Maurer's cleft-associated Plasmodium falciparum membrane-associated histidine-rich protein peptide specifically interacts with the erythrocyte membrane

The membrane-associated histidine-rich protein-1 (MAHRP-1) is a Maurer's cleft-resident molecule that has been recently described as an important protein for the trafficking of PfEMP-1 to infected erythrocyte membrane, a major virulence factor. We have studied the specific interactions between 20-mer-long synthetic peptides spanning the complete MAHRP-1 sequence and erythrocytes. A high-activity binding peptide (HABP) with saturable binding to a 46-kDa erythrocyte membrane protein was identified and its binding was affected by chymotrypsin treatment. Random coil and ?-helical features were found in the HABP's structure. Our results suggest that MAHRP-1 specifically interacts with erythrocyte membrane through a 20-mer-long amino acid region, raising questions about this region's potential as a therapeutic target against malaria. © 2009 Elsevier Inc. All rights reserved.

• Fecha de publicación: 2009
• Institución: Universidad del Rosario
• Repositorio: Repositorio EdocUR - U. Rosario
• Idioma: eng
• OAI Identifier: oai:repository.urosario.edu.co:10336/23505
• Acceso en línea: https://doi.org/10.1016/j.bbrc.2009.01.050; https://repository.urosario.edu.co/handle/10336/23505
• Palabra clave: Carrier proteins and binding proteins; Chymotrypsin; Erythrocyte membrane protein 1; Protein habp; Protein mahrp 1; Protozoal protein; Unclassified drug; Amino acid sequence; Article; Controlled study; Erythrocyte membrane; Nonhuman; Plasmodium falciparum; Priority journal; Protein analysis; Protein binding; Protein interaction; Protein localization; Protein structure; Amino acid sequence; Animals; Carrier proteins; Erythrocyte membrane; Molecular sequence data; Peptides; Plasmodium falciparum; Protozoan proteins; Plasmodium falciparum; Antimalarial candidate; High-activity binding peptide; Mahrp-1; Malaria; Maurer's clefts; Plasmodium falciparum
• License: Abierto (Texto Completo)