Therapeutic Use of the Antimicrobial Peptide PNR20 to Resolve Disseminated Candidiasis in a Murine Model

Invasive fungal infections (IFIs) caused by Candida species are an emerging threat globally, given that patients at-risk and antifungal resistance are increasing. Antimicrobial peptides (AMPs) have shown good therapeutic capacity against different multidrug-resistant (MDR) microorganisms. This study...

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Autores:
Tipo de recurso:
Fecha de publicación:
2023
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/42175
Acceso en línea:
https://repository.urosario.edu.co/handle/10336/42175
Palabra clave:
Candida auris
Candida spp.
Antimicrobial peptides
Resistance
Candidiasis
Rights
License
Attribution-NonCommercial-ShareAlike 4.0 International
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dc.title.spa.fl_str_mv Therapeutic Use of the Antimicrobial Peptide PNR20 to Resolve Disseminated Candidiasis in a Murine Model
title Therapeutic Use of the Antimicrobial Peptide PNR20 to Resolve Disseminated Candidiasis in a Murine Model
spellingShingle Therapeutic Use of the Antimicrobial Peptide PNR20 to Resolve Disseminated Candidiasis in a Murine Model

Candida auris
Candida spp.
Antimicrobial peptides
Resistance
Candidiasis
title_short Therapeutic Use of the Antimicrobial Peptide PNR20 to Resolve Disseminated Candidiasis in a Murine Model
title_full Therapeutic Use of the Antimicrobial Peptide PNR20 to Resolve Disseminated Candidiasis in a Murine Model
title_fullStr Therapeutic Use of the Antimicrobial Peptide PNR20 to Resolve Disseminated Candidiasis in a Murine Model
title_full_unstemmed Therapeutic Use of the Antimicrobial Peptide PNR20 to Resolve Disseminated Candidiasis in a Murine Model
title_sort Therapeutic Use of the Antimicrobial Peptide PNR20 to Resolve Disseminated Candidiasis in a Murine Model
dc.creator.spa.fl_str_mv
author
author_facet
author_role author
dc.subject.spa.fl_str_mv Candida auris
Candida spp.
Antimicrobial peptides
Resistance
Candidiasis
topic Candida auris
Candida spp.
Antimicrobial peptides
Resistance
Candidiasis
description Invasive fungal infections (IFIs) caused by Candida species are an emerging threat globally, given that patients at-risk and antifungal resistance are increasing. Antimicrobial peptides (AMPs) have shown good therapeutic capacity against different multidrug-resistant (MDR) microorganisms. This study evaluated the activity of the synthetic peptide, PNR20, against Candida albicans ATCC 10231 and a MDR Colombian clinical isolate of Candida auris. Perturbation of yeast cell surface was evaluated using scanning electron microscopy. Cell viability of Vero cells was determined to assess peptide toxicity. Additionally, survival, fungal burden, and histopathology of BALB/c mice infected intravenously with each Candida species and treated with PNR20 were analyzed. Morphological alterations were identified in both species, demonstrating the antifungal effect of PNR20. In vitro, Vero cells’ viability was not affected by PNR20. All mice infected with either C. albicans or C. aurisand treated with PNR20 survived and had a significant reduction in the fungal burden in the kidney compared to the control group. The histopathological analysis in mice infected and treated with PNR20 showed more preserved tissues, without the presence of yeast, compared to the control groups. This work shows that the utilization of PNR20 is a promising therapeutic alternative against disseminated candidiasis.
publishDate 2023
dc.date.created.spa.fl_str_mv 2023-12-01
dc.date.issued.spa.fl_str_mv 2023
dc.date.accessioned.none.fl_str_mv 2024-01-31T18:35:53Z
dc.date.available.none.fl_str_mv 2024-01-31T18:35:53Z
dc.type.spa.fl_str_mv article
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dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.spa.fl_str_mv 10.3390/jof9121149
dc.identifier.issn.spa.fl_str_mv 2309-608X
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/42175
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dc.language.iso.spa.fl_str_mv eng
language eng
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dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
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rights_invalid_str_mv Attribution-NonCommercial-ShareAlike 4.0 International
Abierto (Texto Completo)
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http://purl.org/coar/access_right/c_abf2
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dc.publisher.spa.fl_str_mv Universidad del Rosario
dc.source.spa.fl_str_mv Journal of Fungi
institution Universidad del Rosario
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dc.source.reponame.spa.fl_str_mv reponame:Repositorio Institucional EdocUR
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