Therapeutic Use of the Antimicrobial Peptide PNR20 to Resolve Disseminated Candidiasis in a Murine Model
Invasive fungal infections (IFIs) caused by Candida species are an emerging threat globally, given that patients at-risk and antifungal resistance are increasing. Antimicrobial peptides (AMPs) have shown good therapeutic capacity against different multidrug-resistant (MDR) microorganisms. This study...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2023
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/42175
- Acceso en línea:
- https://repository.urosario.edu.co/handle/10336/42175
- Palabra clave:
- Candida auris
Candida spp.
Antimicrobial peptides
Resistance
Candidiasis
- Rights
- License
- Attribution-NonCommercial-ShareAlike 4.0 International
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5253bc4c-9b54-4ac0-b1ef-6f8e8d03c3561732f406-6491-4485-947d-83702ad7cfe1eca483d3-a229-42c8-8a6d-3b5e6f002e5b34d38329-bb82-4719-a3c4-b339eafa17734006ae6f356-80a6-4e45-a359-5f1518edb34c4b1785ee-53ef-4121-be5f-a0483a46b241d83acbec-f2e1-4cc0-9106-bbd514d2edf82024-01-31T18:35:53Z2024-01-31T18:35:53Z2023-12-012023Invasive fungal infections (IFIs) caused by Candida species are an emerging threat globally, given that patients at-risk and antifungal resistance are increasing. Antimicrobial peptides (AMPs) have shown good therapeutic capacity against different multidrug-resistant (MDR) microorganisms. This study evaluated the activity of the synthetic peptide, PNR20, against Candida albicans ATCC 10231 and a MDR Colombian clinical isolate of Candida auris. Perturbation of yeast cell surface was evaluated using scanning electron microscopy. Cell viability of Vero cells was determined to assess peptide toxicity. Additionally, survival, fungal burden, and histopathology of BALB/c mice infected intravenously with each Candida species and treated with PNR20 were analyzed. Morphological alterations were identified in both species, demonstrating the antifungal effect of PNR20. In vitro, Vero cells’ viability was not affected by PNR20. All mice infected with either C. albicans or C. aurisand treated with PNR20 survived and had a significant reduction in the fungal burden in the kidney compared to the control group. The histopathological analysis in mice infected and treated with PNR20 showed more preserved tissues, without the presence of yeast, compared to the control groups. This work shows that the utilization of PNR20 is a promising therapeutic alternative against disseminated candidiasis.application/pdf10.3390/jof91211492309-608Xhttps://repository.urosario.edu.co/handle/10336/42175engUniversidad del Rosariohttps://www.mdpi.com/2309-608X/9/12/1149Attribution-NonCommercial-ShareAlike 4.0 InternationalAbierto (Texto Completo)http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2Journal of Fungiinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURCandida aurisCandida spp.Antimicrobial peptidesResistanceCandidiasisTherapeutic Use of the Antimicrobial Peptide PNR20 to Resolve Disseminated Candidiasis in a Murine ModelarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Micelly-Moreno, J.Barreto-Santamaría, A.Arévalo-Pinzón, GabrielaGómez, Beatríz.L.Escandón, Patricia.Muñoz R. JulianORIGINALTherapeutic Use of the Antimicrobial.pdfapplication/pdf3045522https://repository.urosario.edu.co/bitstreams/eaa84020-35d6-4544-9693-5f68d2a1d93f/downloadc35d6ef318efc056521bab93905805cdMD51TEXTTherapeutic Use of the Antimicrobial.pdf.txtTherapeutic Use of the Antimicrobial.pdf.txtExtracted texttext/plain65477https://repository.urosario.edu.co/bitstreams/ca4bfecb-73b0-4052-ab57-d284bf3db807/download645e4e5e2520f4f1926e59a9830a34d3MD52THUMBNAILTherapeutic Use of the Antimicrobial.pdf.jpgTherapeutic Use of the Antimicrobial.pdf.jpgGenerated Thumbnailimage/jpeg4885https://repository.urosario.edu.co/bitstreams/1dc1b759-07dc-4404-bc2d-aeabe7cf8828/download782e9fe64d1b42e92fc394e52a13c73bMD5310336/42175oai:repository.urosario.edu.co:10336/421752024-02-01 03:04:56.534http://creativecommons.org/licenses/by/4.0/Attribution-NonCommercial-ShareAlike 4.0 Internationalhttps://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |
dc.title.spa.fl_str_mv |
Therapeutic Use of the Antimicrobial Peptide PNR20 to Resolve Disseminated Candidiasis in a Murine Model |
title |
Therapeutic Use of the Antimicrobial Peptide PNR20 to Resolve Disseminated Candidiasis in a Murine Model |
spellingShingle |
Therapeutic Use of the Antimicrobial Peptide PNR20 to Resolve Disseminated Candidiasis in a Murine Model Candida auris Candida spp. Antimicrobial peptides Resistance Candidiasis |
title_short |
Therapeutic Use of the Antimicrobial Peptide PNR20 to Resolve Disseminated Candidiasis in a Murine Model |
title_full |
Therapeutic Use of the Antimicrobial Peptide PNR20 to Resolve Disseminated Candidiasis in a Murine Model |
title_fullStr |
Therapeutic Use of the Antimicrobial Peptide PNR20 to Resolve Disseminated Candidiasis in a Murine Model |
title_full_unstemmed |
Therapeutic Use of the Antimicrobial Peptide PNR20 to Resolve Disseminated Candidiasis in a Murine Model |
title_sort |
Therapeutic Use of the Antimicrobial Peptide PNR20 to Resolve Disseminated Candidiasis in a Murine Model |
dc.creator.spa.fl_str_mv |
|
author |
|
author_facet |
|
author_role |
author |
dc.subject.spa.fl_str_mv |
Candida auris Candida spp. Antimicrobial peptides Resistance Candidiasis |
topic |
Candida auris Candida spp. Antimicrobial peptides Resistance Candidiasis |
description |
Invasive fungal infections (IFIs) caused by Candida species are an emerging threat globally, given that patients at-risk and antifungal resistance are increasing. Antimicrobial peptides (AMPs) have shown good therapeutic capacity against different multidrug-resistant (MDR) microorganisms. This study evaluated the activity of the synthetic peptide, PNR20, against Candida albicans ATCC 10231 and a MDR Colombian clinical isolate of Candida auris. Perturbation of yeast cell surface was evaluated using scanning electron microscopy. Cell viability of Vero cells was determined to assess peptide toxicity. Additionally, survival, fungal burden, and histopathology of BALB/c mice infected intravenously with each Candida species and treated with PNR20 were analyzed. Morphological alterations were identified in both species, demonstrating the antifungal effect of PNR20. In vitro, Vero cells’ viability was not affected by PNR20. All mice infected with either C. albicans or C. aurisand treated with PNR20 survived and had a significant reduction in the fungal burden in the kidney compared to the control group. The histopathological analysis in mice infected and treated with PNR20 showed more preserved tissues, without the presence of yeast, compared to the control groups. This work shows that the utilization of PNR20 is a promising therapeutic alternative against disseminated candidiasis. |
publishDate |
2023 |
dc.date.created.spa.fl_str_mv |
2023-12-01 |
dc.date.issued.spa.fl_str_mv |
2023 |
dc.date.accessioned.none.fl_str_mv |
2024-01-31T18:35:53Z |
dc.date.available.none.fl_str_mv |
2024-01-31T18:35:53Z |
dc.type.spa.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.spa.fl_str_mv |
10.3390/jof9121149 |
dc.identifier.issn.spa.fl_str_mv |
2309-608X |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/42175 |
identifier_str_mv |
10.3390/jof9121149 2309-608X |
url |
https://repository.urosario.edu.co/handle/10336/42175 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.uri.spa.fl_str_mv |
https://www.mdpi.com/2309-608X/9/12/1149 |
dc.rights.spa.fl_str_mv |
Attribution-NonCommercial-ShareAlike 4.0 International |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.acceso.spa.fl_str_mv |
Abierto (Texto Completo) |
dc.rights.uri.spa.fl_str_mv |
http://creativecommons.org/licenses/by/4.0/ |
rights_invalid_str_mv |
Attribution-NonCommercial-ShareAlike 4.0 International Abierto (Texto Completo) http://creativecommons.org/licenses/by/4.0/ http://purl.org/coar/access_right/c_abf2 |
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application/pdf |
dc.publisher.spa.fl_str_mv |
Universidad del Rosario |
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Journal of Fungi |
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Universidad del Rosario |
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instname:Universidad del Rosario |
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reponame:Repositorio Institucional EdocUR |
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