The high immunogenicity induced by modified sporozoites' malarial peptides depends on their phi (?) and psi (?) angles

The importance of CSP- and STARP-derived ? and ? dihedral angles in mHABP structure was analysed by 1H NMR in the search for molecules which can be included as components of a first-line-of-defence Plasmodium falciparum sporozoite multi-epitope vaccine against the most lethal form of human malaria. Most of the aforementioned dihedral angles were left-hand-like polyproline type II (PPIIL) structures whilst others had right-hand-like ?-helix (?R), thus allowing mHABPS to fit better into MHCII molecules and thereby form an appropriate pMHCII complex and also establish the H-bonds which stabilise such complex and by this means induce an appropriate immune response. This information has great implications for vaccine development, malaria being one of them. © 2012 Elsevier Inc.

• Fecha de publicación: 2012
• Institución: Universidad del Rosario
• Repositorio: Repositorio EdocUR - U. Rosario
• Idioma: eng
• OAI Identifier: oai:repository.urosario.edu.co:10336/23512
• Acceso en línea: https://doi.org/10.1016/j.bbrc.2012.10.088; https://repository.urosario.edu.co/handle/10336/23512
• Palabra clave: Binding protein; Circumsporozoite protein; High activity binding peptide; Major histocompatibility antigen class 2; Proline; Protozoal protein; Sporozoite threonine and asparagine rich protein; Sporozoite vaccine; Unclassified drug; Alpha helix; Amino acid sequence; Article; Complex formation; Hydrogen bond; Immunogenicity; Plasmodium falciparum; Priority journal; Protein structure; Proton nuclear magnetic resonance; Amino acid sequence; Animals; Aotus trivirgatus; Hla-dr beta-chains; Humans; Malaria vaccines; Molecular sequence data; Peptide fragments; Peptides; Plasmodium falciparum; Protozoan proteins; Sporozoites; Plasmodium falciparum; ? and ? angles; Antimalarial-vaccine; Hladr?* molecules; Plasmodium falciparum; Pre-erythrocyte stage; secondary; protozoan; biomolecular; Antigens; Nuclear magnetic resonance; Protein structure
• License: Abierto (Texto Completo)