Sexual forms obtained in a continuous in vitro cultured Colombian strain of Plasmodium falciparum (FCB2)

Background: The epidemiological control of malaria has been hampered by the appearance of parasite resistance to anti-malarial drugs and by the resistance of mosquito vectors to control measures. This has also been associated with weak transmission control, mostly due to poor control of asymptomatic...

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Autores:
Tipo de recurso:
Fecha de publicación:
2020
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/22550
Acceso en línea:
https://doi.org/10.1186/s12936-020-3142-y
https://repository.urosario.edu.co/handle/10336/22550
Palabra clave:
Antimalarial agent
Malaria vaccine
Animal cell
Anopheles
Anopheles albimanus
Anopheles albimanus infection
Article
Colombia
Comparative study
Controlled study
Erythrocyte
Female
Gametocyte
Gene
Gene expression
Genetic analysis
Human
Human cell
In vitro study
Incubation time
Infection
Low temperature
Microbial morphology
Midgut
Nonhuman
Oocyst
Parasite examination
Parasite transmission
Pfap2g gene
Pfg25 gene
Pfg27 gene
Pfs16 gene
Pfs25 gene
Plasmodium falciparum
Plasmodium falciparum 3d7
Plasmodium falciparum fcb2
Real time polymerase chain reaction
Sex differentiation
Stimulus
Fcb2
Gametocyte
Malaria
Mosquito infectivity
Oocyst
Plasmodium falciparum
Sexual differentiation
Rights
License
Abierto (Texto Completo)
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dc.title.spa.fl_str_mv Sexual forms obtained in a continuous in vitro cultured Colombian strain of Plasmodium falciparum (FCB2)
title Sexual forms obtained in a continuous in vitro cultured Colombian strain of Plasmodium falciparum (FCB2)
spellingShingle Sexual forms obtained in a continuous in vitro cultured Colombian strain of Plasmodium falciparum (FCB2)
Antimalarial agent
Malaria vaccine
Animal cell
Anopheles
Anopheles albimanus
Anopheles albimanus infection
Article
Colombia
Comparative study
Controlled study
Erythrocyte
Female
Gametocyte
Gene
Gene expression
Genetic analysis
Human
Human cell
In vitro study
Incubation time
Infection
Low temperature
Microbial morphology
Midgut
Nonhuman
Oocyst
Parasite examination
Parasite transmission
Pfap2g gene
Pfg25 gene
Pfg27 gene
Pfs16 gene
Pfs25 gene
Plasmodium falciparum
Plasmodium falciparum 3d7
Plasmodium falciparum fcb2
Real time polymerase chain reaction
Sex differentiation
Stimulus
Fcb2
Gametocyte
Malaria
Mosquito infectivity
Oocyst
Plasmodium falciparum
Sexual differentiation
title_short Sexual forms obtained in a continuous in vitro cultured Colombian strain of Plasmodium falciparum (FCB2)
title_full Sexual forms obtained in a continuous in vitro cultured Colombian strain of Plasmodium falciparum (FCB2)
title_fullStr Sexual forms obtained in a continuous in vitro cultured Colombian strain of Plasmodium falciparum (FCB2)
title_full_unstemmed Sexual forms obtained in a continuous in vitro cultured Colombian strain of Plasmodium falciparum (FCB2)
title_sort Sexual forms obtained in a continuous in vitro cultured Colombian strain of Plasmodium falciparum (FCB2)
dc.subject.keyword.spa.fl_str_mv Antimalarial agent
Malaria vaccine
Animal cell
Anopheles
Anopheles albimanus
Anopheles albimanus infection
Article
Colombia
Comparative study
Controlled study
Erythrocyte
Female
Gametocyte
Gene
Gene expression
Genetic analysis
Human
Human cell
In vitro study
Incubation time
Infection
Low temperature
Microbial morphology
Midgut
Nonhuman
Oocyst
Parasite examination
Parasite transmission
Pfap2g gene
Pfg25 gene
Pfg27 gene
Pfs16 gene
Pfs25 gene
Plasmodium falciparum
Plasmodium falciparum 3d7
Plasmodium falciparum fcb2
Real time polymerase chain reaction
Sex differentiation
Stimulus
Fcb2
Gametocyte
Malaria
Mosquito infectivity
Oocyst
Plasmodium falciparum
Sexual differentiation
topic Antimalarial agent
Malaria vaccine
Animal cell
Anopheles
Anopheles albimanus
Anopheles albimanus infection
Article
Colombia
Comparative study
Controlled study
Erythrocyte
Female
Gametocyte
Gene
Gene expression
Genetic analysis
Human
Human cell
In vitro study
Incubation time
Infection
Low temperature
Microbial morphology
Midgut
Nonhuman
Oocyst
Parasite examination
Parasite transmission
Pfap2g gene
Pfg25 gene
Pfg27 gene
Pfs16 gene
Pfs25 gene
Plasmodium falciparum
Plasmodium falciparum 3d7
Plasmodium falciparum fcb2
Real time polymerase chain reaction
Sex differentiation
Stimulus
Fcb2
Gametocyte
Malaria
Mosquito infectivity
Oocyst
Plasmodium falciparum
Sexual differentiation
description Background: The epidemiological control of malaria has been hampered by the appearance of parasite resistance to anti-malarial drugs and by the resistance of mosquito vectors to control measures. This has also been associated with weak transmission control, mostly due to poor control of asymptomatic patients associated with host-vector transmission. This highlights the importance of studying the parasite's sexual forms (gametocytes) which are involved in this phase of the parasite's life-cycle. Some African and Asian strains of Plasmodium falciparum have been fully characterized regarding sexual forms' production; however, few Latin-American strains have been so characterized. This study was aimed at characterizing the Colombian FCB2 strain as a gametocyte producer able to infect mosquitoes. Methods: Gametocyte production was induced in in vitro cultured P. falciparum FCB2 and 3D7 strains. Pfap2g and Pfs25 gene expression was detected in FCB2 strain gametocyte culture by RT-PCR. Comparative analysis of gametocytes obtained from both strains was made (counts and morphological changes). In vitro zygote formation from FCB2 gametocytes was induced by incubating a gametocyte culture sample at 27 °C for 20 min. A controlled Anopheles albimanus infection was made using an artificial feed system with cultured FCB2 gametocytes (14-15 days old). Mosquito midgut dissection was then carried out for analyzing oocysts. Results: The FCB2 strain expressed Pfap2g, Pfs16, Pfg27/25 and Pfs25 sexual differentiation-related genes after in vitro sexual differentiation induction, producing gametocytes that conserved the expected morphological features. The amount of FCB2 gametocytes produced was similar to that from the 3D7 strain. FCB2 gametocytes were differentiated into zygotes and ookinetes after an in vitro low-temperature stimulus and infected An. albimanus mosquitoes, developing to oocyst stage. Conclusions: Even with the history of long-term FCB2 strain in vitro culture maintenance, it has retained its sexual differentiation ability. The gametocytes produced here preserved these parasite forms' usual characteristics and An. albimanus infection capability, thus enabling its use as a tool for studying sexual form biology, An. albimanus infection comparative analysis and anti-malarial drug and vaccine development. © 2020 The Author(s).
publishDate 2020
dc.date.accessioned.none.fl_str_mv 2020-05-25T23:56:53Z
dc.date.available.none.fl_str_mv 2020-05-25T23:56:53Z
dc.date.created.spa.fl_str_mv 2020
dc.type.eng.fl_str_mv article
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dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1186/s12936-020-3142-y
dc.identifier.issn.none.fl_str_mv 14752875
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/22550
url https://doi.org/10.1186/s12936-020-3142-y
https://repository.urosario.edu.co/handle/10336/22550
identifier_str_mv 14752875
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationIssue.none.fl_str_mv No. 1
dc.relation.citationTitle.none.fl_str_mv Malaria Journal
dc.relation.citationVolume.none.fl_str_mv Vol. 19
dc.relation.ispartof.spa.fl_str_mv Malaria Journal, ISSN:14752875, Vol.19, No.1 (2020)
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dc.publisher.spa.fl_str_mv BioMed Central Ltd.
institution Universidad del Rosario
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dc.source.reponame.spa.fl_str_mv reponame:Repositorio Institucional EdocUR
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