A rapid and sensitive assay for histone acetyl-transferase activity

Histone acetyl-transferases (HATs) seem to be key elements in the regulation of transcription. We have designed an enzymatic assay to quantify HAT enzymatic activity. In this assay, the substrate is a peptide corresponding to the 24 first amino acids of histone H4 which is coupled to biotin. After a...

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Autores:
Tipo de recurso:
Fecha de publicación:
1998
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/27775
Acceso en línea:
https://doi.org/10.1093/nar/26.16.3869
https://repository.urosario.edu.co/handle/10336/27775
Palabra clave:
HATs
Enzymes
Peptide
Rights
License
Abierto (Texto Completo)
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spelling 56600cc5-d451-4c09-9961-1395cf5c65cb51866251600c42342f4-1e6f-42b0-9209-a5640e729742dfbae35f-65e0-498a-9b8d-6e3ae5cba5201646dccd-922b-4f40-b27d-cdac6c6e9c622020-08-19T14:43:49Z2020-08-19T14:43:49Z1998-08-01Histone acetyl-transferases (HATs) seem to be key elements in the regulation of transcription. We have designed an enzymatic assay to quantify HAT enzymatic activity. In this assay, the substrate is a peptide corresponding to the 24 first amino acids of histone H4 which is coupled to biotin. After acetylation using [14C]acetyl-CoA, the peptide is purified on streptavidin beads and the associated radioactivity is measured. This assay is sensitive, rapid and convenient.application/pdfhttps://doi.org/10.1093/nar/26.16.3869ISSN: 0305-1048EISSN: 1362-4962https://repository.urosario.edu.co/handle/10336/27775engOxford University Press3870No. 163869Nucleic Acids ResearchVol. 26Nucleic Acids Research, ISSN: 0305-1048;EISSN: 1362-4962, Vol.26, No.16 (1 August 1998); pp. 3869–3870https://watermark.silverchair.com/26-16-3869.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAq4wggKqBgkqhkiG9w0BBwagggKbMIIClwIBADCCApAGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMimF92GpOeCO7A1OkAgEQgIICYQZsEVMIAXB0Y-56NtOexbqJAJnxIKDkCVqfk-UM_axWffOKl75mA-Gw4FUb5hb6cgkVhBukhhhsQzBo3Pn5BOh8fca5jDWhQTWsncSqhLAYgyfaq9wbAk4hSJvwkUvlktYq7SDYMUhIa8GoEdp1Zl0NI_cxoi5TszeAfORhH4scta8CZcoBRh076KeslNVAs6tt6u9DoWSnkoO_RvUwmOzK_JPDtWhwQT2VsE1n-jsqjty7K3bnxoJIysFJBM78ul3blTh14k8oE_gMYoOJNCVnZDpnYuq1mlNKk8XcBvPluCmPADuymVCtxSoIOudSw_OM0Rhg2MsxDDoTxG-JYtc94AFfIp8qnNQE7Dib4O5Ho-YBRkNTroKRVxgLD2e97Qg2s05f4-o3xmuV1DkZfW6tnjKCX5zFcj2UerStnFETDF8NS4vaIoKruNBQc7aOdIl7CtS4OooA6tEbVsM8ZYgLbL8d935RtdiljSoMqT4wH_pcF3-rrHf8RPUxc6jbilhLVMuzzxwqwdcm8uO4XmgNhq4X5U4m8RuaF93K5Wfm8kpRW7z5j8oVh6R0kG8cAytMkuNNcjZSGjtRcETKceY47GVvGv1G8vHWLU32KM7FomndOUeBCg0OUkpsEa0ZKsmsTxzW9ae_rsioNwHIGpavOyYrYuZB-WCt3M59roKYFK_Or1O8S2u7ZzXYqemcamWubnsUvSyg3Ks8by2pE1DP9Zy7Ul8WwvFeB-a34jK8U2QxDUzGROipyS8tWCRrAds6NrwJMnZlesPPIiMDcruZKZiDT98j626g1efKKTVKzgAbierto (Texto Completo)http://purl.org/coar/access_right/c_abf2Nucleic Acids Researchinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURHATsEnzymesPeptideA rapid and sensitive assay for histone acetyl-transferase activityUn ensayo rápido y sensible para la actividad de la histona acetiltransferasaarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Ait-Si-Ali, S.Ramírez Clavijo, Sandra RocíoRobin, P.Trouche, D.Harel-Bellan, A.ORIGINAL26-16-3869.pdfapplication/pdf62297https://repository.urosario.edu.co/bitstreams/5f2f9839-e9b1-4ccf-8e53-44ef1552eeb6/download30ba7fef9e1c4ab445797e51aa1631d1MD51TEXT26-16-3869.pdf.txt26-16-3869.pdf.txtExtracted texttext/plain8962https://repository.urosario.edu.co/bitstreams/812c549f-0cb1-43c0-8e51-32727c69ff30/download61f230272bdeda2865db2f52e3202278MD52THUMBNAIL26-16-3869.pdf.jpg26-16-3869.pdf.jpgGenerated Thumbnailimage/jpeg5154https://repository.urosario.edu.co/bitstreams/44f4ff1a-996e-4ec1-a568-4b5614f9fb2c/download0bb3f34f0bf3db29c2e6f29f2eda7939MD5310336/27775oai:repository.urosario.edu.co:10336/277752021-10-05 16:41:28.483https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv A rapid and sensitive assay for histone acetyl-transferase activity
dc.title.TranslatedTitle.spa.fl_str_mv Un ensayo rápido y sensible para la actividad de la histona acetiltransferasa
title A rapid and sensitive assay for histone acetyl-transferase activity
spellingShingle A rapid and sensitive assay for histone acetyl-transferase activity
HATs
Enzymes
Peptide
title_short A rapid and sensitive assay for histone acetyl-transferase activity
title_full A rapid and sensitive assay for histone acetyl-transferase activity
title_fullStr A rapid and sensitive assay for histone acetyl-transferase activity
title_full_unstemmed A rapid and sensitive assay for histone acetyl-transferase activity
title_sort A rapid and sensitive assay for histone acetyl-transferase activity
dc.subject.keyword.spa.fl_str_mv HATs
Enzymes
Peptide
topic HATs
Enzymes
Peptide
description Histone acetyl-transferases (HATs) seem to be key elements in the regulation of transcription. We have designed an enzymatic assay to quantify HAT enzymatic activity. In this assay, the substrate is a peptide corresponding to the 24 first amino acids of histone H4 which is coupled to biotin. After acetylation using [14C]acetyl-CoA, the peptide is purified on streptavidin beads and the associated radioactivity is measured. This assay is sensitive, rapid and convenient.
publishDate 1998
dc.date.created.spa.fl_str_mv 1998-08-01
dc.date.accessioned.none.fl_str_mv 2020-08-19T14:43:49Z
dc.date.available.none.fl_str_mv 2020-08-19T14:43:49Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
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dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1093/nar/26.16.3869
dc.identifier.issn.none.fl_str_mv ISSN: 0305-1048
EISSN: 1362-4962
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/27775
url https://doi.org/10.1093/nar/26.16.3869
https://repository.urosario.edu.co/handle/10336/27775
identifier_str_mv ISSN: 0305-1048
EISSN: 1362-4962
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 3870
dc.relation.citationIssue.none.fl_str_mv No. 16
dc.relation.citationStartPage.none.fl_str_mv 3869
dc.relation.citationTitle.none.fl_str_mv Nucleic Acids Research
dc.relation.citationVolume.none.fl_str_mv Vol. 26
dc.relation.ispartof.spa.fl_str_mv Nucleic Acids Research, ISSN: 0305-1048;EISSN: 1362-4962, Vol.26, No.16 (1 August 1998); pp. 3869–3870
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dc.publisher.spa.fl_str_mv Oxford University Press
dc.source.spa.fl_str_mv Nucleic Acids Research
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