A rapid and sensitive assay for histone acetyl-transferase activity
Histone acetyl-transferases (HATs) seem to be key elements in the regulation of transcription. We have designed an enzymatic assay to quantify HAT enzymatic activity. In this assay, the substrate is a peptide corresponding to the 24 first amino acids of histone H4 which is coupled to biotin. After a...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 1998
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/27775
- Acceso en línea:
- https://doi.org/10.1093/nar/26.16.3869
https://repository.urosario.edu.co/handle/10336/27775
- Palabra clave:
- HATs
Enzymes
Peptide
- Rights
- License
- Abierto (Texto Completo)
| Summary: | Histone acetyl-transferases (HATs) seem to be key elements in the regulation of transcription. We have designed an enzymatic assay to quantify HAT enzymatic activity. In this assay, the substrate is a peptide corresponding to the 24 first amino acids of histone H4 which is coupled to biotin. After acetylation using [14C]acetyl-CoA, the peptide is purified on streptavidin beads and the associated radioactivity is measured. This assay is sensitive, rapid and convenient. |
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