Evaluation of microphthalmia associated transcription factor (MITF) expression in peripheral blood of a population with malign melanoma and control population and cell lines

Background: The incidence of malign melanoma tumours has increased more rapidly than any other type of cancer; this has intensified the searching for tools that facilitate early detection of melanoma. Microphthalmia associated transcription factor (MITF) is currently known as being a master melanocy...

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Autores:
Tipo de recurso:
Fecha de publicación:
2009
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/23728
Acceso en línea:
https://repository.urosario.edu.co/handle/10336/23728
Palabra clave:
Microphthalmia associated transcription factor
Rna
Article
Blood sampling
Cancer cell culture
Circulation
Clinical article
Controlled study
Gene expression
Housekeeping gene
Human
Melanoma
Protein blood level
Real time polymerase chain reaction
Rna extraction
Stomach adenocarcinoma
Tumor cell
Cancer
Circulation tumor cells
Melanoma
Mitf
Qpcr
Rights
License
Abierto (Texto Completo)
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network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
spelling 297e20e9-7c4a-48f3-9a53-3f8366a352c86bc0f0d6-9b4d-4f7a-848d-9768a9a5c6b0518662516002020-05-26T00:04:52Z2020-05-26T00:04:52Z2009Background: The incidence of malign melanoma tumours has increased more rapidly than any other type of cancer; this has intensified the searching for tools that facilitate early detection of melanoma. Microphthalmia associated transcription factor (MITF) is currently known as being a master melanocyte regulator. The article analyses MITF gene expression in peripheral blood of individuals suffering from melanoma, compared to people without any type of cancer and some cell lines. Materials and methods: Thirty one samples of peripheral blood were used: 19 from patients having melanoma and 12 from healthy people. Then RNA was extracted from these samples. MITF and housekeeping genes (?2M and GAPDH) expression levels were then quantified by real-time PCR. Five cell lines were also used to determine the MITF expression. Results: MITF gene expression could be observed in all individuals, though no statistical significant differences were found among expression levels in the groups studied (p=0.09). Even so, MITF expression in the group of patients suffering from melanoma was much more variable than that observed in the group of cancer-free people. Expression was detected in the cell line AGS (gastric adenocarcinoma), not yet described. Conclusions: MITF gene expression levels were detected in the peripheral blood from both people suffering from melanoma and people without any type of cancer. However, variability in the number of molecules in MITF gene expression was observed in people with melanoma, this suggests the presence of tumour cells in circulation. © 2009 Universidad del Valle, Facultad de Salud.application/pdf1657953401208322https://repository.urosario.edu.co/handle/10336/23728eng33No. 116Colombia MedicaVol. 40Colombia Medica, ISSN:16579534, 01208322, Vol.40, No.1 (2009); pp. 16-33https://www.scopus.com/inward/record.uri?eid=2-s2.0-64549093414&partnerID=40&md5=3a75af35d9c919580d61ed839cbae02fAbierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURMicrophthalmia associated transcription factorRnaArticleBlood samplingCancer cell cultureCirculationClinical articleControlled studyGene expressionHousekeeping geneHumanMelanomaProtein blood levelReal time polymerase chain reactionRna extractionStomach adenocarcinomaTumor cellCancerCirculation tumor cellsMelanomaMitfQpcrEvaluation of microphthalmia associated transcription factor (MITF) expression in peripheral blood of a population with malign melanoma and control population and cell linesEvaluacuón de la expresión del factor de transcripción asociado con microftalmia (MITF) en sangre periférica de individuos con y sin melanoma maligno y en líneas celularesarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Rangel N.Rondón M.Ramírez Clavijo, Sandra RocíoORIGINAL626-Manuscript-1902-1-10-20091202.pdfapplication/pdf231200https://repository.urosario.edu.co/bitstreams/fa7db47a-a643-426c-a053-14705c732d35/downloadbcafec7f8c6275a4355bcad568878105MD51TEXT626-Manuscript-1902-1-10-20091202.pdf.txt626-Manuscript-1902-1-10-20091202.pdf.txtExtracted texttext/plain34738https://repository.urosario.edu.co/bitstreams/884f290e-3bbb-4f18-a52b-d9a6d787096d/download3f46b96ae984ad0f111759489c027ea0MD52THUMBNAIL626-Manuscript-1902-1-10-20091202.pdf.jpg626-Manuscript-1902-1-10-20091202.pdf.jpgGenerated Thumbnailimage/jpeg4052https://repository.urosario.edu.co/bitstreams/dec369e4-4f6e-41ff-910c-e38a7a7cb557/downloadf874523acce2489772580a9455f3ead7MD5310336/23728oai:repository.urosario.edu.co:10336/237282022-05-02 07:37:16.61528https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Evaluation of microphthalmia associated transcription factor (MITF) expression in peripheral blood of a population with malign melanoma and control population and cell lines
dc.title.TranslatedTitle.spa.fl_str_mv Evaluacuón de la expresión del factor de transcripción asociado con microftalmia (MITF) en sangre periférica de individuos con y sin melanoma maligno y en líneas celulares
title Evaluation of microphthalmia associated transcription factor (MITF) expression in peripheral blood of a population with malign melanoma and control population and cell lines
spellingShingle Evaluation of microphthalmia associated transcription factor (MITF) expression in peripheral blood of a population with malign melanoma and control population and cell lines
Microphthalmia associated transcription factor
Rna
Article
Blood sampling
Cancer cell culture
Circulation
Clinical article
Controlled study
Gene expression
Housekeeping gene
Human
Melanoma
Protein blood level
Real time polymerase chain reaction
Rna extraction
Stomach adenocarcinoma
Tumor cell
Cancer
Circulation tumor cells
Melanoma
Mitf
Qpcr
title_short Evaluation of microphthalmia associated transcription factor (MITF) expression in peripheral blood of a population with malign melanoma and control population and cell lines
title_full Evaluation of microphthalmia associated transcription factor (MITF) expression in peripheral blood of a population with malign melanoma and control population and cell lines
title_fullStr Evaluation of microphthalmia associated transcription factor (MITF) expression in peripheral blood of a population with malign melanoma and control population and cell lines
title_full_unstemmed Evaluation of microphthalmia associated transcription factor (MITF) expression in peripheral blood of a population with malign melanoma and control population and cell lines
title_sort Evaluation of microphthalmia associated transcription factor (MITF) expression in peripheral blood of a population with malign melanoma and control population and cell lines
dc.subject.keyword.spa.fl_str_mv Microphthalmia associated transcription factor
Rna
Article
Blood sampling
Cancer cell culture
Circulation
Clinical article
Controlled study
Gene expression
Housekeeping gene
Human
Melanoma
Protein blood level
Real time polymerase chain reaction
Rna extraction
Stomach adenocarcinoma
Tumor cell
Cancer
Circulation tumor cells
Melanoma
Mitf
Qpcr
topic Microphthalmia associated transcription factor
Rna
Article
Blood sampling
Cancer cell culture
Circulation
Clinical article
Controlled study
Gene expression
Housekeeping gene
Human
Melanoma
Protein blood level
Real time polymerase chain reaction
Rna extraction
Stomach adenocarcinoma
Tumor cell
Cancer
Circulation tumor cells
Melanoma
Mitf
Qpcr
description Background: The incidence of malign melanoma tumours has increased more rapidly than any other type of cancer; this has intensified the searching for tools that facilitate early detection of melanoma. Microphthalmia associated transcription factor (MITF) is currently known as being a master melanocyte regulator. The article analyses MITF gene expression in peripheral blood of individuals suffering from melanoma, compared to people without any type of cancer and some cell lines. Materials and methods: Thirty one samples of peripheral blood were used: 19 from patients having melanoma and 12 from healthy people. Then RNA was extracted from these samples. MITF and housekeeping genes (?2M and GAPDH) expression levels were then quantified by real-time PCR. Five cell lines were also used to determine the MITF expression. Results: MITF gene expression could be observed in all individuals, though no statistical significant differences were found among expression levels in the groups studied (p=0.09). Even so, MITF expression in the group of patients suffering from melanoma was much more variable than that observed in the group of cancer-free people. Expression was detected in the cell line AGS (gastric adenocarcinoma), not yet described. Conclusions: MITF gene expression levels were detected in the peripheral blood from both people suffering from melanoma and people without any type of cancer. However, variability in the number of molecules in MITF gene expression was observed in people with melanoma, this suggests the presence of tumour cells in circulation. © 2009 Universidad del Valle, Facultad de Salud.
publishDate 2009
dc.date.created.spa.fl_str_mv 2009
dc.date.accessioned.none.fl_str_mv 2020-05-26T00:04:52Z
dc.date.available.none.fl_str_mv 2020-05-26T00:04:52Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.issn.none.fl_str_mv 16579534
01208322
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/23728
identifier_str_mv 16579534
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url https://repository.urosario.edu.co/handle/10336/23728
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 33
dc.relation.citationIssue.none.fl_str_mv No. 1
dc.relation.citationStartPage.none.fl_str_mv 16
dc.relation.citationTitle.none.fl_str_mv Colombia Medica
dc.relation.citationVolume.none.fl_str_mv Vol. 40
dc.relation.ispartof.spa.fl_str_mv Colombia Medica, ISSN:16579534, 01208322, Vol.40, No.1 (2009); pp. 16-33
dc.relation.uri.spa.fl_str_mv https://www.scopus.com/inward/record.uri?eid=2-s2.0-64549093414&partnerID=40&md5=3a75af35d9c919580d61ed839cbae02f
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rights_invalid_str_mv Abierto (Texto Completo)
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institution Universidad del Rosario
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