Evaluation of microphthalmia associated transcription factor (MITF) expression in peripheral blood of a population with malign melanoma and control population and cell lines
Background: The incidence of malign melanoma tumours has increased more rapidly than any other type of cancer; this has intensified the searching for tools that facilitate early detection of melanoma. Microphthalmia associated transcription factor (MITF) is currently known as being a master melanocy...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2009
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/23728
- Acceso en línea:
- https://repository.urosario.edu.co/handle/10336/23728
- Palabra clave:
- Microphthalmia associated transcription factor
Rna
Article
Blood sampling
Cancer cell culture
Circulation
Clinical article
Controlled study
Gene expression
Housekeeping gene
Human
Melanoma
Protein blood level
Real time polymerase chain reaction
Rna extraction
Stomach adenocarcinoma
Tumor cell
Cancer
Circulation tumor cells
Melanoma
Mitf
Qpcr
- Rights
- License
- Abierto (Texto Completo)
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297e20e9-7c4a-48f3-9a53-3f8366a352c86bc0f0d6-9b4d-4f7a-848d-9768a9a5c6b0518662516002020-05-26T00:04:52Z2020-05-26T00:04:52Z2009Background: The incidence of malign melanoma tumours has increased more rapidly than any other type of cancer; this has intensified the searching for tools that facilitate early detection of melanoma. Microphthalmia associated transcription factor (MITF) is currently known as being a master melanocyte regulator. The article analyses MITF gene expression in peripheral blood of individuals suffering from melanoma, compared to people without any type of cancer and some cell lines. Materials and methods: Thirty one samples of peripheral blood were used: 19 from patients having melanoma and 12 from healthy people. Then RNA was extracted from these samples. MITF and housekeeping genes (?2M and GAPDH) expression levels were then quantified by real-time PCR. Five cell lines were also used to determine the MITF expression. Results: MITF gene expression could be observed in all individuals, though no statistical significant differences were found among expression levels in the groups studied (p=0.09). Even so, MITF expression in the group of patients suffering from melanoma was much more variable than that observed in the group of cancer-free people. Expression was detected in the cell line AGS (gastric adenocarcinoma), not yet described. Conclusions: MITF gene expression levels were detected in the peripheral blood from both people suffering from melanoma and people without any type of cancer. However, variability in the number of molecules in MITF gene expression was observed in people with melanoma, this suggests the presence of tumour cells in circulation. © 2009 Universidad del Valle, Facultad de Salud.application/pdf1657953401208322https://repository.urosario.edu.co/handle/10336/23728eng33No. 116Colombia MedicaVol. 40Colombia Medica, ISSN:16579534, 01208322, Vol.40, No.1 (2009); pp. 16-33https://www.scopus.com/inward/record.uri?eid=2-s2.0-64549093414&partnerID=40&md5=3a75af35d9c919580d61ed839cbae02fAbierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURMicrophthalmia associated transcription factorRnaArticleBlood samplingCancer cell cultureCirculationClinical articleControlled studyGene expressionHousekeeping geneHumanMelanomaProtein blood levelReal time polymerase chain reactionRna extractionStomach adenocarcinomaTumor cellCancerCirculation tumor cellsMelanomaMitfQpcrEvaluation of microphthalmia associated transcription factor (MITF) expression in peripheral blood of a population with malign melanoma and control population and cell linesEvaluacuón de la expresión del factor de transcripción asociado con microftalmia (MITF) en sangre periférica de individuos con y sin melanoma maligno y en líneas celularesarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Rangel N.Rondón M.Ramírez Clavijo, Sandra RocíoORIGINAL626-Manuscript-1902-1-10-20091202.pdfapplication/pdf231200https://repository.urosario.edu.co/bitstreams/fa7db47a-a643-426c-a053-14705c732d35/downloadbcafec7f8c6275a4355bcad568878105MD51TEXT626-Manuscript-1902-1-10-20091202.pdf.txt626-Manuscript-1902-1-10-20091202.pdf.txtExtracted texttext/plain34738https://repository.urosario.edu.co/bitstreams/884f290e-3bbb-4f18-a52b-d9a6d787096d/download3f46b96ae984ad0f111759489c027ea0MD52THUMBNAIL626-Manuscript-1902-1-10-20091202.pdf.jpg626-Manuscript-1902-1-10-20091202.pdf.jpgGenerated Thumbnailimage/jpeg4052https://repository.urosario.edu.co/bitstreams/dec369e4-4f6e-41ff-910c-e38a7a7cb557/downloadf874523acce2489772580a9455f3ead7MD5310336/23728oai:repository.urosario.edu.co:10336/237282022-05-02 07:37:16.61528https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |
dc.title.spa.fl_str_mv |
Evaluation of microphthalmia associated transcription factor (MITF) expression in peripheral blood of a population with malign melanoma and control population and cell lines |
dc.title.TranslatedTitle.spa.fl_str_mv |
Evaluacuón de la expresión del factor de transcripción asociado con microftalmia (MITF) en sangre periférica de individuos con y sin melanoma maligno y en líneas celulares |
title |
Evaluation of microphthalmia associated transcription factor (MITF) expression in peripheral blood of a population with malign melanoma and control population and cell lines |
spellingShingle |
Evaluation of microphthalmia associated transcription factor (MITF) expression in peripheral blood of a population with malign melanoma and control population and cell lines Microphthalmia associated transcription factor Rna Article Blood sampling Cancer cell culture Circulation Clinical article Controlled study Gene expression Housekeeping gene Human Melanoma Protein blood level Real time polymerase chain reaction Rna extraction Stomach adenocarcinoma Tumor cell Cancer Circulation tumor cells Melanoma Mitf Qpcr |
title_short |
Evaluation of microphthalmia associated transcription factor (MITF) expression in peripheral blood of a population with malign melanoma and control population and cell lines |
title_full |
Evaluation of microphthalmia associated transcription factor (MITF) expression in peripheral blood of a population with malign melanoma and control population and cell lines |
title_fullStr |
Evaluation of microphthalmia associated transcription factor (MITF) expression in peripheral blood of a population with malign melanoma and control population and cell lines |
title_full_unstemmed |
Evaluation of microphthalmia associated transcription factor (MITF) expression in peripheral blood of a population with malign melanoma and control population and cell lines |
title_sort |
Evaluation of microphthalmia associated transcription factor (MITF) expression in peripheral blood of a population with malign melanoma and control population and cell lines |
dc.subject.keyword.spa.fl_str_mv |
Microphthalmia associated transcription factor Rna Article Blood sampling Cancer cell culture Circulation Clinical article Controlled study Gene expression Housekeeping gene Human Melanoma Protein blood level Real time polymerase chain reaction Rna extraction Stomach adenocarcinoma Tumor cell Cancer Circulation tumor cells Melanoma Mitf Qpcr |
topic |
Microphthalmia associated transcription factor Rna Article Blood sampling Cancer cell culture Circulation Clinical article Controlled study Gene expression Housekeeping gene Human Melanoma Protein blood level Real time polymerase chain reaction Rna extraction Stomach adenocarcinoma Tumor cell Cancer Circulation tumor cells Melanoma Mitf Qpcr |
description |
Background: The incidence of malign melanoma tumours has increased more rapidly than any other type of cancer; this has intensified the searching for tools that facilitate early detection of melanoma. Microphthalmia associated transcription factor (MITF) is currently known as being a master melanocyte regulator. The article analyses MITF gene expression in peripheral blood of individuals suffering from melanoma, compared to people without any type of cancer and some cell lines. Materials and methods: Thirty one samples of peripheral blood were used: 19 from patients having melanoma and 12 from healthy people. Then RNA was extracted from these samples. MITF and housekeeping genes (?2M and GAPDH) expression levels were then quantified by real-time PCR. Five cell lines were also used to determine the MITF expression. Results: MITF gene expression could be observed in all individuals, though no statistical significant differences were found among expression levels in the groups studied (p=0.09). Even so, MITF expression in the group of patients suffering from melanoma was much more variable than that observed in the group of cancer-free people. Expression was detected in the cell line AGS (gastric adenocarcinoma), not yet described. Conclusions: MITF gene expression levels were detected in the peripheral blood from both people suffering from melanoma and people without any type of cancer. However, variability in the number of molecules in MITF gene expression was observed in people with melanoma, this suggests the presence of tumour cells in circulation. © 2009 Universidad del Valle, Facultad de Salud. |
publishDate |
2009 |
dc.date.created.spa.fl_str_mv |
2009 |
dc.date.accessioned.none.fl_str_mv |
2020-05-26T00:04:52Z |
dc.date.available.none.fl_str_mv |
2020-05-26T00:04:52Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.issn.none.fl_str_mv |
16579534 01208322 |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/23728 |
identifier_str_mv |
16579534 01208322 |
url |
https://repository.urosario.edu.co/handle/10336/23728 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.citationEndPage.none.fl_str_mv |
33 |
dc.relation.citationIssue.none.fl_str_mv |
No. 1 |
dc.relation.citationStartPage.none.fl_str_mv |
16 |
dc.relation.citationTitle.none.fl_str_mv |
Colombia Medica |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 40 |
dc.relation.ispartof.spa.fl_str_mv |
Colombia Medica, ISSN:16579534, 01208322, Vol.40, No.1 (2009); pp. 16-33 |
dc.relation.uri.spa.fl_str_mv |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-64549093414&partnerID=40&md5=3a75af35d9c919580d61ed839cbae02f |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
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Abierto (Texto Completo) |
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Abierto (Texto Completo) http://purl.org/coar/access_right/c_abf2 |
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