In vitro and in vivo studies for assessing the immune response and protection-inducing ability conferred by Fasciola hepatica-derived synthetic peptides containing B- and T-cell epitopes

Fasciolosis is considered the most widespread trematode disease affecting grazing animals around the world; it is currently recognised by the World Health Organisation as an emergent human pathogen. Triclabendazole is still the most effective drug against this disease; however, resistant strains hav...

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Autores:
Tipo de recurso:
Fecha de publicación:
2014
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/18685
Acceso en línea:
https://doi.org/10.1371/journal.pone.0105323
http://repository.urosario.edu.co/handle/10336/18685
Palabra clave:
Fasciola hepática
Enzimología
Parasitología
Cytokine
Epitope
Helminth Antibody
Immunoglobulin G
Peptide
Protozoal Vaccine
Amino Acid Sequence
Animal
Blood
Chemistry
Cluster Analysis
Disease Model
Fasciola Hepatica
Fascioliasis
Female
Gene Expression Profiling
Genetics
Immunology
Metabolism
Mortality
Mouse
Parasitology
Synthesis
Amino Acid Sequence
Animals
Fasciola Hepatica
Fascioliasis
Gene Expression Profiling
Mice
Peptides
Protozoan Vaccines
Animal
T-Lymphocyte
B-Lymphocyte
Disease Models
Epitopes
Epitopes
Rights
License
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Description
Summary:Fasciolosis is considered the most widespread trematode disease affecting grazing animals around the world; it is currently recognised by the World Health Organisation as an emergent human pathogen. Triclabendazole is still the most effective drug against this disease; however, resistant strains have appeared and developing an effective vaccine against this disease has increasingly become a priority. Several bioinformatics tools were here used for predicting B- and T-cell epitopes according to the available data for Fasciola hepatica protein amino acid sequences. BALB/c mice were immunised with the synthetic peptides by using the ADAD vaccination system and several immune response parameters were measured (antibody titres, cytokine levels, T-cell populations) to evaluate their ability to elicit an immune response. Based on the immunogenicity results so obtained, seven peptides were selected to assess their protection-inducing ability against experimental infection with F. hepatica metacercariae. Twenty-four B- or T-epitope-containing peptides were predicted and chemically synthesised. Immunisation of mice with peptides so-called B1, B2, B5, B6, T14, T15 and T16 induced high levels of total IgG, IgG1 and IgG2a (p<0.05) and a mixed Th1/Th2/Th17/Treg immune response, according to IFN-γ, IL-4, IL-17 and IL-10 levels, accompanied by increased CD62L+ T-cell populations. A high level of protection was obtained in mice vaccinated with peptides B2, B5, B6 and T15 formulated in the ADAD vaccination system with the AA0029 immunomodulator. The bioinformatics approach used in the present study led to the identification of seven peptides as vaccine candidates against the infection caused by Fasciola hepatica (a liver-fluke trematode). However, vaccine efficacy must be evaluated in other host species, including those having veterinary importance. © 2014 Rojas-Caraballo et al.