Identification and characterisation of a cDNA encoding a 17-kDa isoform of rat myelin basic protein

The myelin basic proteins (MBPs) are the major proteins of the myelin membrane. Multiple MBP mRNAs and protein isoforms are generated by alternative RNA splicing. Here we describe the isolation and characterisation of a cDNA clone encoding a 17-kDa MBP isoform from the rat (Rattus norvegicus). The i...

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Tipo de recurso:
Fecha de publicación:
2003
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/25960
Acceso en línea:
https://doi.org/10.1016/j.bbaexp.2003.08.010
https://repository.urosario.edu.co/handle/10336/25960
Palabra clave:
Myelin basic protein
cDNA cloning
Developmental expression
Brain
mRNA
Rat
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License
Restringido (Acceso a grupos específicos)
id EDOCUR2_294b0fd7375a8e0d923bf3e76eaecc68
oai_identifier_str oai:repository.urosario.edu.co:10336/25960
network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
spelling 51803354600f419c26e-d62c-476b-b5e7-2c7c2f04b6dc2020-08-06T16:20:19Z2020-08-06T16:20:19Z2003-10-20The myelin basic proteins (MBPs) are the major proteins of the myelin membrane. Multiple MBP mRNAs and protein isoforms are generated by alternative RNA splicing. Here we describe the isolation and characterisation of a cDNA clone encoding a 17-kDa MBP isoform from the rat (Rattus norvegicus). The isoform is a 158-amino acid protein consisting of exons 1, 3, 4, 6 and 7 of the MBP gene. RT-PCR analysis of brain mRNA showed that transcripts encoding the 17-kDa isoform were expressed at higher levels early in post-natal development, up to 7 days post-partum.application/pdfhttps://doi.org/10.1016/j.bbaexp.2003.08.010ISSN: 0167-4781EISSN: 1879-2634https://repository.urosario.edu.co/handle/10336/25960engElsevier53No. 147Biochimica et Biophysica Acta (BBA) - Gene Structure and ExpressionVol. 1630Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, ISSN:0167-4781;EISSN: 1879-2634, Vol.1630, No.1 (2003) pp.47-53https://www.sciencedirect.com/science/article/abs/pii/S0167478103001842Restringido (Acceso a grupos específicos)http://purl.org/coar/access_right/c_16ecBiochimica et Biophysica Acta (BBA) - Gene Structure and Expressioninstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURMyelin basic proteincDNA cloningDevelopmental expressionBrainmRNARatIdentification and characterisation of a cDNA encoding a 17-kDa isoform of rat myelin basic proteinIdentificación y caracterización de un ADNc que codifica una isoforma de 17 kDa de proteína básica de mielina de rataarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Matheus Merino, Luisa MarinaBlair, G. Eric10336/25960oai:repository.urosario.edu.co:10336/259602021-09-13 01:23:11.723https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Identification and characterisation of a cDNA encoding a 17-kDa isoform of rat myelin basic protein
dc.title.TranslatedTitle.spa.fl_str_mv Identificación y caracterización de un ADNc que codifica una isoforma de 17 kDa de proteína básica de mielina de rata
title Identification and characterisation of a cDNA encoding a 17-kDa isoform of rat myelin basic protein
spellingShingle Identification and characterisation of a cDNA encoding a 17-kDa isoform of rat myelin basic protein
Myelin basic protein
cDNA cloning
Developmental expression
Brain
mRNA
Rat
title_short Identification and characterisation of a cDNA encoding a 17-kDa isoform of rat myelin basic protein
title_full Identification and characterisation of a cDNA encoding a 17-kDa isoform of rat myelin basic protein
title_fullStr Identification and characterisation of a cDNA encoding a 17-kDa isoform of rat myelin basic protein
title_full_unstemmed Identification and characterisation of a cDNA encoding a 17-kDa isoform of rat myelin basic protein
title_sort Identification and characterisation of a cDNA encoding a 17-kDa isoform of rat myelin basic protein
dc.subject.keyword.spa.fl_str_mv Myelin basic protein
cDNA cloning
Developmental expression
Brain
mRNA
Rat
topic Myelin basic protein
cDNA cloning
Developmental expression
Brain
mRNA
Rat
description The myelin basic proteins (MBPs) are the major proteins of the myelin membrane. Multiple MBP mRNAs and protein isoforms are generated by alternative RNA splicing. Here we describe the isolation and characterisation of a cDNA clone encoding a 17-kDa MBP isoform from the rat (Rattus norvegicus). The isoform is a 158-amino acid protein consisting of exons 1, 3, 4, 6 and 7 of the MBP gene. RT-PCR analysis of brain mRNA showed that transcripts encoding the 17-kDa isoform were expressed at higher levels early in post-natal development, up to 7 days post-partum.
publishDate 2003
dc.date.created.spa.fl_str_mv 2003-10-20
dc.date.accessioned.none.fl_str_mv 2020-08-06T16:20:19Z
dc.date.available.none.fl_str_mv 2020-08-06T16:20:19Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1016/j.bbaexp.2003.08.010
dc.identifier.issn.none.fl_str_mv ISSN: 0167-4781
EISSN: 1879-2634
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/25960
url https://doi.org/10.1016/j.bbaexp.2003.08.010
https://repository.urosario.edu.co/handle/10336/25960
identifier_str_mv ISSN: 0167-4781
EISSN: 1879-2634
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 53
dc.relation.citationIssue.none.fl_str_mv No. 1
dc.relation.citationStartPage.none.fl_str_mv 47
dc.relation.citationTitle.none.fl_str_mv Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression
dc.relation.citationVolume.none.fl_str_mv Vol. 1630
dc.relation.ispartof.spa.fl_str_mv Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, ISSN:0167-4781;EISSN: 1879-2634, Vol.1630, No.1 (2003) pp.47-53
dc.relation.uri.spa.fl_str_mv https://www.sciencedirect.com/science/article/abs/pii/S0167478103001842
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_16ec
dc.rights.acceso.spa.fl_str_mv Restringido (Acceso a grupos específicos)
rights_invalid_str_mv Restringido (Acceso a grupos específicos)
http://purl.org/coar/access_right/c_16ec
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Elsevier
dc.source.spa.fl_str_mv Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression
institution Universidad del Rosario
dc.source.instname.none.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.none.fl_str_mv reponame:Repositorio Institucional EdocUR
repository.name.fl_str_mv Repositorio institucional EdocUR
repository.mail.fl_str_mv edocur@urosario.edu.co
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