Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina
In this study, we describe the frequency of Acanthamoeba keratitis (AK) in patients that assisted in the Ophthalmology Department and determine the species/genotypes of free living amoebas (FLA) isolates. FLA from Corneal scrapes (CS) and contact lens (CL) wearers were studied by morphological and m...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2017
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/24304
- Acceso en línea:
- https://doi.org/10.1016/j.meegid.2017.06.031
https://repository.urosario.edu.co/handle/10336/24304
- Palabra clave:
- Acanthamoeba keratitis
Acanthamoeba polyphaga
Adult
Aged
Argentina
Article
Contact lens
Controlled study
Cornea
Demography
Disease association
Disease severity
Environmental factor
Eye redness
Female
Genotype phenotype correlation
Human
Human tissue
Major clinical study
Male
Morphology
Nonhuman
Parasite identification
Parasite isolation
Photophobia
Phylogenetic tree
Priority journal
Sequence analysis
Social status
Acanthamoeba
Acanthamoeba keratitis
Classification
Contact lens
Genetics
Genotype
Middle aged
Parasite load
Parasitology
Phylogeny
Very elderly
Acanthamoeba
Acanthamoeba Keratitis
Adult
Aged
Aged, 80 and over
Argentina
Contact Lenses
Female
Genotype
Humans
Male
Middle Aged
Parasite Load
Phylogeny
Acanthamoeba
Argentina
Contact lens
Genotype
Keratitis
- Rights
- License
- Abierto (Texto Completo)
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ef33b241-53ab-4dc5-ac9a-12f8dfe002375c3469da-b624-4400-91a7-117356441bfa681a9041-805e-46cb-aca9-7a90e027069b7cd3df44-511d-43b0-9e23-b8526066194dbcac1aa0-838c-49b8-855e-938890133ab4a7a226c4-009f-458b-9617-a78d965500944ded0f64-16ed-4bb5-8fc9-392470215d3010117161186002020-05-26T00:11:28Z2020-05-26T00:11:28Z2017In this study, we describe the frequency of Acanthamoeba keratitis (AK) in patients that assisted in the Ophthalmology Department and determine the species/genotypes of free living amoebas (FLA) isolates. FLA from Corneal scrapes (CS) and contact lens (CL) wearers were studied by morphological and molecular characterization. A database was constructed with sociodemographic, clinical findings and history of use of CL variables. During January 2000 and September 2016 patients with corneal pathology admitted to the Ophthalmology Service of the University Hospital in Córdoba city, Argentina were included in the study. FLA were detected in 1.5% (11/739) and in 17% (11/65) of CS and CL analyzed respectively. FLA isolates from CL users evidenced an 80.9% of inappropriate lens maintenance, 4.8% (1/21) were not CL users that have been in contact with waters in outdoor environment and 14,3% (3/21) with no data about CL users. Acanthamoeba was confirmed in 100% and 82% of CS and LC respectively. The most frequent symptom associated with AK was red eye and photophobia. FLA from CS belonged to group II but 82% (9/11) and 18% (2/11) from CL belonged to group II and III respectively. T4 genotype and A. polyphaga species were detected in 100% of Acanthamoeba isolates. Poor CL hygiene practices, highlights the need for improved education about the severity of AK and consequences of improper CL hygiene. Genotype T4 detected in 100% of both CS and CL samples, consistently with previous findings indicating that this genotype is by far the most prevalent isolated from ocular infection. © 2017 Elsevier B.V.application/pdfhttps://doi.org/10.1016/j.meegid.2017.06.03115671348https://repository.urosario.edu.co/handle/10336/24304engElsevier B.V.175170Infection, Genetics and EvolutionVol. 54Infection, Genetics and Evolution, ISSN:15671348, Vol.54,(2017); pp. 170-175https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021950076&doi=10.1016%2fj.meegid.2017.06.031&partnerID=40&md5=598b0f96a6d0b51ab656bf83cdcbd1d5Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURAcanthamoeba keratitisAcanthamoeba polyphagaAdultAgedArgentinaArticleContact lensControlled studyCorneaDemographyDisease associationDisease severityEnvironmental factorEye rednessFemaleGenotype phenotype correlationHumanHuman tissueMajor clinical studyMaleMorphologyNonhumanParasite identificationParasite isolationPhotophobiaPhylogenetic treePriority journalSequence analysisSocial statusAcanthamoebaAcanthamoeba keratitisClassificationContact lensGeneticsGenotypeMiddle agedParasite loadParasitologyPhylogenyVery elderlyAcanthamoebaAcanthamoeba KeratitisAdultAgedAged, 80 and overArgentinaContact LensesFemaleGenotypeHumansMaleMiddle AgedParasite LoadPhylogenyAcanthamoebaArgentinaContact lensGenotypeKeratitisMolecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in ArgentinaarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Casero R.D.Mongi F.Laconte L.Rivero F.Sastre D.Teherán A.Herrera G.Ramírez, Juan David10336/24304oai:repository.urosario.edu.co:10336/243042022-05-02 07:37:16.484564https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |
dc.title.spa.fl_str_mv |
Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina |
title |
Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina |
spellingShingle |
Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina Acanthamoeba keratitis Acanthamoeba polyphaga Adult Aged Argentina Article Contact lens Controlled study Cornea Demography Disease association Disease severity Environmental factor Eye redness Female Genotype phenotype correlation Human Human tissue Major clinical study Male Morphology Nonhuman Parasite identification Parasite isolation Photophobia Phylogenetic tree Priority journal Sequence analysis Social status Acanthamoeba Acanthamoeba keratitis Classification Contact lens Genetics Genotype Middle aged Parasite load Parasitology Phylogeny Very elderly Acanthamoeba Acanthamoeba Keratitis Adult Aged Aged, 80 and over Argentina Contact Lenses Female Genotype Humans Male Middle Aged Parasite Load Phylogeny Acanthamoeba Argentina Contact lens Genotype Keratitis |
title_short |
Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina |
title_full |
Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina |
title_fullStr |
Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina |
title_full_unstemmed |
Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina |
title_sort |
Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina |
dc.subject.keyword.spa.fl_str_mv |
Acanthamoeba keratitis Acanthamoeba polyphaga Adult Aged Argentina Article Contact lens Controlled study Cornea Demography Disease association Disease severity Environmental factor Eye redness Female Genotype phenotype correlation Human Human tissue Major clinical study Male Morphology Nonhuman Parasite identification Parasite isolation Photophobia Phylogenetic tree Priority journal Sequence analysis Social status Acanthamoeba Acanthamoeba keratitis Classification Contact lens Genetics Genotype Middle aged Parasite load Parasitology Phylogeny Very elderly Acanthamoeba Acanthamoeba Keratitis Adult Aged Aged, 80 and over Argentina Contact Lenses Female Genotype Humans Male Middle Aged Parasite Load Phylogeny Acanthamoeba Argentina Contact lens Genotype Keratitis |
topic |
Acanthamoeba keratitis Acanthamoeba polyphaga Adult Aged Argentina Article Contact lens Controlled study Cornea Demography Disease association Disease severity Environmental factor Eye redness Female Genotype phenotype correlation Human Human tissue Major clinical study Male Morphology Nonhuman Parasite identification Parasite isolation Photophobia Phylogenetic tree Priority journal Sequence analysis Social status Acanthamoeba Acanthamoeba keratitis Classification Contact lens Genetics Genotype Middle aged Parasite load Parasitology Phylogeny Very elderly Acanthamoeba Acanthamoeba Keratitis Adult Aged Aged, 80 and over Argentina Contact Lenses Female Genotype Humans Male Middle Aged Parasite Load Phylogeny Acanthamoeba Argentina Contact lens Genotype Keratitis |
description |
In this study, we describe the frequency of Acanthamoeba keratitis (AK) in patients that assisted in the Ophthalmology Department and determine the species/genotypes of free living amoebas (FLA) isolates. FLA from Corneal scrapes (CS) and contact lens (CL) wearers were studied by morphological and molecular characterization. A database was constructed with sociodemographic, clinical findings and history of use of CL variables. During January 2000 and September 2016 patients with corneal pathology admitted to the Ophthalmology Service of the University Hospital in Córdoba city, Argentina were included in the study. FLA were detected in 1.5% (11/739) and in 17% (11/65) of CS and CL analyzed respectively. FLA isolates from CL users evidenced an 80.9% of inappropriate lens maintenance, 4.8% (1/21) were not CL users that have been in contact with waters in outdoor environment and 14,3% (3/21) with no data about CL users. Acanthamoeba was confirmed in 100% and 82% of CS and LC respectively. The most frequent symptom associated with AK was red eye and photophobia. FLA from CS belonged to group II but 82% (9/11) and 18% (2/11) from CL belonged to group II and III respectively. T4 genotype and A. polyphaga species were detected in 100% of Acanthamoeba isolates. Poor CL hygiene practices, highlights the need for improved education about the severity of AK and consequences of improper CL hygiene. Genotype T4 detected in 100% of both CS and CL samples, consistently with previous findings indicating that this genotype is by far the most prevalent isolated from ocular infection. © 2017 Elsevier B.V. |
publishDate |
2017 |
dc.date.created.spa.fl_str_mv |
2017 |
dc.date.accessioned.none.fl_str_mv |
2020-05-26T00:11:28Z |
dc.date.available.none.fl_str_mv |
2020-05-26T00:11:28Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1016/j.meegid.2017.06.031 |
dc.identifier.issn.none.fl_str_mv |
15671348 |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/24304 |
url |
https://doi.org/10.1016/j.meegid.2017.06.031 https://repository.urosario.edu.co/handle/10336/24304 |
identifier_str_mv |
15671348 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.citationEndPage.none.fl_str_mv |
175 |
dc.relation.citationStartPage.none.fl_str_mv |
170 |
dc.relation.citationTitle.none.fl_str_mv |
Infection, Genetics and Evolution |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 54 |
dc.relation.ispartof.spa.fl_str_mv |
Infection, Genetics and Evolution, ISSN:15671348, Vol.54,(2017); pp. 170-175 |
dc.relation.uri.spa.fl_str_mv |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021950076&doi=10.1016%2fj.meegid.2017.06.031&partnerID=40&md5=598b0f96a6d0b51ab656bf83cdcbd1d5 |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.acceso.spa.fl_str_mv |
Abierto (Texto Completo) |
rights_invalid_str_mv |
Abierto (Texto Completo) http://purl.org/coar/access_right/c_abf2 |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Elsevier B.V. |
institution |
Universidad del Rosario |
dc.source.instname.spa.fl_str_mv |
instname:Universidad del Rosario |
dc.source.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional EdocUR |
repository.name.fl_str_mv |
Repositorio institucional EdocUR |
repository.mail.fl_str_mv |
edocur@urosario.edu.co |
_version_ |
1814167738805911552 |