Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina

In this study, we describe the frequency of Acanthamoeba keratitis (AK) in patients that assisted in the Ophthalmology Department and determine the species/genotypes of free living amoebas (FLA) isolates. FLA from Corneal scrapes (CS) and contact lens (CL) wearers were studied by morphological and m...

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Autores:
Tipo de recurso:
Fecha de publicación:
2017
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/24304
Acceso en línea:
https://doi.org/10.1016/j.meegid.2017.06.031
https://repository.urosario.edu.co/handle/10336/24304
Palabra clave:
Acanthamoeba keratitis
Acanthamoeba polyphaga
Adult
Aged
Argentina
Article
Contact lens
Controlled study
Cornea
Demography
Disease association
Disease severity
Environmental factor
Eye redness
Female
Genotype phenotype correlation
Human
Human tissue
Major clinical study
Male
Morphology
Nonhuman
Parasite identification
Parasite isolation
Photophobia
Phylogenetic tree
Priority journal
Sequence analysis
Social status
Acanthamoeba
Acanthamoeba keratitis
Classification
Contact lens
Genetics
Genotype
Middle aged
Parasite load
Parasitology
Phylogeny
Very elderly
Acanthamoeba
Acanthamoeba Keratitis
Adult
Aged
Aged, 80 and over
Argentina
Contact Lenses
Female
Genotype
Humans
Male
Middle Aged
Parasite Load
Phylogeny
Acanthamoeba
Argentina
Contact lens
Genotype
Keratitis
Rights
License
Abierto (Texto Completo)
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network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
spelling ef33b241-53ab-4dc5-ac9a-12f8dfe002375c3469da-b624-4400-91a7-117356441bfa681a9041-805e-46cb-aca9-7a90e027069b7cd3df44-511d-43b0-9e23-b8526066194dbcac1aa0-838c-49b8-855e-938890133ab4a7a226c4-009f-458b-9617-a78d965500944ded0f64-16ed-4bb5-8fc9-392470215d3010117161186002020-05-26T00:11:28Z2020-05-26T00:11:28Z2017In this study, we describe the frequency of Acanthamoeba keratitis (AK) in patients that assisted in the Ophthalmology Department and determine the species/genotypes of free living amoebas (FLA) isolates. FLA from Corneal scrapes (CS) and contact lens (CL) wearers were studied by morphological and molecular characterization. A database was constructed with sociodemographic, clinical findings and history of use of CL variables. During January 2000 and September 2016 patients with corneal pathology admitted to the Ophthalmology Service of the University Hospital in Córdoba city, Argentina were included in the study. FLA were detected in 1.5% (11/739) and in 17% (11/65) of CS and CL analyzed respectively. FLA isolates from CL users evidenced an 80.9% of inappropriate lens maintenance, 4.8% (1/21) were not CL users that have been in contact with waters in outdoor environment and 14,3% (3/21) with no data about CL users. Acanthamoeba was confirmed in 100% and 82% of CS and LC respectively. The most frequent symptom associated with AK was red eye and photophobia. FLA from CS belonged to group II but 82% (9/11) and 18% (2/11) from CL belonged to group II and III respectively. T4 genotype and A. polyphaga species were detected in 100% of Acanthamoeba isolates. Poor CL hygiene practices, highlights the need for improved education about the severity of AK and consequences of improper CL hygiene. Genotype T4 detected in 100% of both CS and CL samples, consistently with previous findings indicating that this genotype is by far the most prevalent isolated from ocular infection. © 2017 Elsevier B.V.application/pdfhttps://doi.org/10.1016/j.meegid.2017.06.03115671348https://repository.urosario.edu.co/handle/10336/24304engElsevier B.V.175170Infection, Genetics and EvolutionVol. 54Infection, Genetics and Evolution, ISSN:15671348, Vol.54,(2017); pp. 170-175https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021950076&doi=10.1016%2fj.meegid.2017.06.031&partnerID=40&md5=598b0f96a6d0b51ab656bf83cdcbd1d5Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURAcanthamoeba keratitisAcanthamoeba polyphagaAdultAgedArgentinaArticleContact lensControlled studyCorneaDemographyDisease associationDisease severityEnvironmental factorEye rednessFemaleGenotype phenotype correlationHumanHuman tissueMajor clinical studyMaleMorphologyNonhumanParasite identificationParasite isolationPhotophobiaPhylogenetic treePriority journalSequence analysisSocial statusAcanthamoebaAcanthamoeba keratitisClassificationContact lensGeneticsGenotypeMiddle agedParasite loadParasitologyPhylogenyVery elderlyAcanthamoebaAcanthamoeba KeratitisAdultAgedAged, 80 and overArgentinaContact LensesFemaleGenotypeHumansMaleMiddle AgedParasite LoadPhylogenyAcanthamoebaArgentinaContact lensGenotypeKeratitisMolecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in ArgentinaarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Casero R.D.Mongi F.Laconte L.Rivero F.Sastre D.Teherán A.Herrera G.Ramírez, Juan David10336/24304oai:repository.urosario.edu.co:10336/243042022-05-02 07:37:16.484564https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina
title Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina
spellingShingle Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina
Acanthamoeba keratitis
Acanthamoeba polyphaga
Adult
Aged
Argentina
Article
Contact lens
Controlled study
Cornea
Demography
Disease association
Disease severity
Environmental factor
Eye redness
Female
Genotype phenotype correlation
Human
Human tissue
Major clinical study
Male
Morphology
Nonhuman
Parasite identification
Parasite isolation
Photophobia
Phylogenetic tree
Priority journal
Sequence analysis
Social status
Acanthamoeba
Acanthamoeba keratitis
Classification
Contact lens
Genetics
Genotype
Middle aged
Parasite load
Parasitology
Phylogeny
Very elderly
Acanthamoeba
Acanthamoeba Keratitis
Adult
Aged
Aged, 80 and over
Argentina
Contact Lenses
Female
Genotype
Humans
Male
Middle Aged
Parasite Load
Phylogeny
Acanthamoeba
Argentina
Contact lens
Genotype
Keratitis
title_short Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina
title_full Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina
title_fullStr Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina
title_full_unstemmed Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina
title_sort Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina
dc.subject.keyword.spa.fl_str_mv Acanthamoeba keratitis
Acanthamoeba polyphaga
Adult
Aged
Argentina
Article
Contact lens
Controlled study
Cornea
Demography
Disease association
Disease severity
Environmental factor
Eye redness
Female
Genotype phenotype correlation
Human
Human tissue
Major clinical study
Male
Morphology
Nonhuman
Parasite identification
Parasite isolation
Photophobia
Phylogenetic tree
Priority journal
Sequence analysis
Social status
Acanthamoeba
Acanthamoeba keratitis
Classification
Contact lens
Genetics
Genotype
Middle aged
Parasite load
Parasitology
Phylogeny
Very elderly
Acanthamoeba
Acanthamoeba Keratitis
Adult
Aged
Aged, 80 and over
Argentina
Contact Lenses
Female
Genotype
Humans
Male
Middle Aged
Parasite Load
Phylogeny
Acanthamoeba
Argentina
Contact lens
Genotype
Keratitis
topic Acanthamoeba keratitis
Acanthamoeba polyphaga
Adult
Aged
Argentina
Article
Contact lens
Controlled study
Cornea
Demography
Disease association
Disease severity
Environmental factor
Eye redness
Female
Genotype phenotype correlation
Human
Human tissue
Major clinical study
Male
Morphology
Nonhuman
Parasite identification
Parasite isolation
Photophobia
Phylogenetic tree
Priority journal
Sequence analysis
Social status
Acanthamoeba
Acanthamoeba keratitis
Classification
Contact lens
Genetics
Genotype
Middle aged
Parasite load
Parasitology
Phylogeny
Very elderly
Acanthamoeba
Acanthamoeba Keratitis
Adult
Aged
Aged, 80 and over
Argentina
Contact Lenses
Female
Genotype
Humans
Male
Middle Aged
Parasite Load
Phylogeny
Acanthamoeba
Argentina
Contact lens
Genotype
Keratitis
description In this study, we describe the frequency of Acanthamoeba keratitis (AK) in patients that assisted in the Ophthalmology Department and determine the species/genotypes of free living amoebas (FLA) isolates. FLA from Corneal scrapes (CS) and contact lens (CL) wearers were studied by morphological and molecular characterization. A database was constructed with sociodemographic, clinical findings and history of use of CL variables. During January 2000 and September 2016 patients with corneal pathology admitted to the Ophthalmology Service of the University Hospital in Córdoba city, Argentina were included in the study. FLA were detected in 1.5% (11/739) and in 17% (11/65) of CS and CL analyzed respectively. FLA isolates from CL users evidenced an 80.9% of inappropriate lens maintenance, 4.8% (1/21) were not CL users that have been in contact with waters in outdoor environment and 14,3% (3/21) with no data about CL users. Acanthamoeba was confirmed in 100% and 82% of CS and LC respectively. The most frequent symptom associated with AK was red eye and photophobia. FLA from CS belonged to group II but 82% (9/11) and 18% (2/11) from CL belonged to group II and III respectively. T4 genotype and A. polyphaga species were detected in 100% of Acanthamoeba isolates. Poor CL hygiene practices, highlights the need for improved education about the severity of AK and consequences of improper CL hygiene. Genotype T4 detected in 100% of both CS and CL samples, consistently with previous findings indicating that this genotype is by far the most prevalent isolated from ocular infection. © 2017 Elsevier B.V.
publishDate 2017
dc.date.created.spa.fl_str_mv 2017
dc.date.accessioned.none.fl_str_mv 2020-05-26T00:11:28Z
dc.date.available.none.fl_str_mv 2020-05-26T00:11:28Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1016/j.meegid.2017.06.031
dc.identifier.issn.none.fl_str_mv 15671348
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/24304
url https://doi.org/10.1016/j.meegid.2017.06.031
https://repository.urosario.edu.co/handle/10336/24304
identifier_str_mv 15671348
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 175
dc.relation.citationStartPage.none.fl_str_mv 170
dc.relation.citationTitle.none.fl_str_mv Infection, Genetics and Evolution
dc.relation.citationVolume.none.fl_str_mv Vol. 54
dc.relation.ispartof.spa.fl_str_mv Infection, Genetics and Evolution, ISSN:15671348, Vol.54,(2017); pp. 170-175
dc.relation.uri.spa.fl_str_mv https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021950076&doi=10.1016%2fj.meegid.2017.06.031&partnerID=40&md5=598b0f96a6d0b51ab656bf83cdcbd1d5
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
rights_invalid_str_mv Abierto (Texto Completo)
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Elsevier B.V.
institution Universidad del Rosario
dc.source.instname.spa.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.spa.fl_str_mv reponame:Repositorio Institucional EdocUR
repository.name.fl_str_mv Repositorio institucional EdocUR
repository.mail.fl_str_mv edocur@urosario.edu.co
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