The in vitro antigenicity of Plasmodium vivax rhoptry neck protein 2 (PvRON2) B- and T-epitopes selected by HLA-DRB1 binding profile

Malaria caused by Plasmodium vivax is a neglected disease which is responsible for the highest morbidity in both Americas and Asia. Despite continuous public health efforts to prevent malarial infection, an effective antimalarial vaccine is still urgently needed. P. vivax vaccine development involve...

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Autores:
Tipo de recurso:
Fecha de publicación:
2018
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/24079
Acceso en línea:
https://doi.org/10.3389/fcimb.2018.00156
https://repository.urosario.edu.co/handle/10336/24079
Palabra clave:
Alkaline phosphatase
Epitope
Gamma interferon
Hla drb1 antigen
Interleukin 10
Interleukin 6
Plasmodium vivax rhoptry neck protein 2
Protein
Streptavidin
Tumor necrosis factor
Unclassified drug
Cytokine
Epitope
Hla drb1 antigen
Immunoglobulin g
Parasite antigen
Peptide
Protozoal protein
Antigenicity
Article
Bioinformatics
Cell isolation
Cell proliferation
Cytokine release
Enzyme linked immunospot assay
Human
Human cell
Humoral immunity
Ic50
Immune response
Immunofluorescence
Peripheral blood mononuclear cell
Plasmodium vivax
Western blotting
Biology
Blood
Colombia
Immunology
Metabolism
Plasmodium falciparum
Plasmodium vivax
Plasmodium vivax malaria
Cell proliferation
Colombia
Computational biology
Cytokines
Hla-drb1 chains
Humans
Immunoglobulin g
Inhibitory concentration 50
Interferon-gamma
Interleukin-10
Interleukin-6
Peptides
Plasmodium falciparum
Plasmodium vivax
Protozoan proteins
Antigenicity
Cellular and humoral response
Epitope
Hla-drb1 typing
Plasmodium vivax
Pvron2
Synthetic peptide
humoral
vivax
t-lymphocyte
protozoan
b-lymphocyte
Antigens
Epitopes
Epitopes
Immunity
Malaria
Rights
License
Abierto (Texto Completo)
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network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
dc.title.spa.fl_str_mv The in vitro antigenicity of Plasmodium vivax rhoptry neck protein 2 (PvRON2) B- and T-epitopes selected by HLA-DRB1 binding profile
title The in vitro antigenicity of Plasmodium vivax rhoptry neck protein 2 (PvRON2) B- and T-epitopes selected by HLA-DRB1 binding profile
spellingShingle The in vitro antigenicity of Plasmodium vivax rhoptry neck protein 2 (PvRON2) B- and T-epitopes selected by HLA-DRB1 binding profile
Alkaline phosphatase
Epitope
Gamma interferon
Hla drb1 antigen
Interleukin 10
Interleukin 6
Plasmodium vivax rhoptry neck protein 2
Protein
Streptavidin
Tumor necrosis factor
Unclassified drug
Cytokine
Epitope
Hla drb1 antigen
Immunoglobulin g
Parasite antigen
Peptide
Protozoal protein
Antigenicity
Article
Bioinformatics
Cell isolation
Cell proliferation
Cytokine release
Enzyme linked immunospot assay
Human
Human cell
Humoral immunity
Ic50
Immune response
Immunofluorescence
Peripheral blood mononuclear cell
Plasmodium vivax
Western blotting
Biology
Blood
Colombia
Immunology
Metabolism
Plasmodium falciparum
Plasmodium vivax
Plasmodium vivax malaria
Cell proliferation
Colombia
Computational biology
Cytokines
Hla-drb1 chains
Humans
Immunoglobulin g
Inhibitory concentration 50
Interferon-gamma
Interleukin-10
Interleukin-6
Peptides
Plasmodium falciparum
Plasmodium vivax
Protozoan proteins
Antigenicity
Cellular and humoral response
Epitope
Hla-drb1 typing
Plasmodium vivax
Pvron2
Synthetic peptide
humoral
vivax
t-lymphocyte
protozoan
b-lymphocyte
Antigens
Epitopes
Epitopes
Immunity
Malaria
title_short The in vitro antigenicity of Plasmodium vivax rhoptry neck protein 2 (PvRON2) B- and T-epitopes selected by HLA-DRB1 binding profile
title_full The in vitro antigenicity of Plasmodium vivax rhoptry neck protein 2 (PvRON2) B- and T-epitopes selected by HLA-DRB1 binding profile
title_fullStr The in vitro antigenicity of Plasmodium vivax rhoptry neck protein 2 (PvRON2) B- and T-epitopes selected by HLA-DRB1 binding profile
title_full_unstemmed The in vitro antigenicity of Plasmodium vivax rhoptry neck protein 2 (PvRON2) B- and T-epitopes selected by HLA-DRB1 binding profile
title_sort The in vitro antigenicity of Plasmodium vivax rhoptry neck protein 2 (PvRON2) B- and T-epitopes selected by HLA-DRB1 binding profile
dc.subject.keyword.spa.fl_str_mv Alkaline phosphatase
Epitope
Gamma interferon
Hla drb1 antigen
Interleukin 10
Interleukin 6
Plasmodium vivax rhoptry neck protein 2
Protein
Streptavidin
Tumor necrosis factor
Unclassified drug
Cytokine
Epitope
Hla drb1 antigen
Immunoglobulin g
Parasite antigen
Peptide
Protozoal protein
Antigenicity
Article
Bioinformatics
Cell isolation
Cell proliferation
Cytokine release
Enzyme linked immunospot assay
Human
Human cell
Humoral immunity
Ic50
Immune response
Immunofluorescence
Peripheral blood mononuclear cell
Plasmodium vivax
Western blotting
Biology
Blood
Colombia
Immunology
Metabolism
Plasmodium falciparum
Plasmodium vivax
Plasmodium vivax malaria
Cell proliferation
Colombia
Computational biology
Cytokines
Hla-drb1 chains
Humans
Immunoglobulin g
Inhibitory concentration 50
Interferon-gamma
Interleukin-10
Interleukin-6
Peptides
Plasmodium falciparum
Plasmodium vivax
Protozoan proteins
Antigenicity
Cellular and humoral response
Epitope
Hla-drb1 typing
Plasmodium vivax
Pvron2
Synthetic peptide
topic Alkaline phosphatase
Epitope
Gamma interferon
Hla drb1 antigen
Interleukin 10
Interleukin 6
Plasmodium vivax rhoptry neck protein 2
Protein
Streptavidin
Tumor necrosis factor
Unclassified drug
Cytokine
Epitope
Hla drb1 antigen
Immunoglobulin g
Parasite antigen
Peptide
Protozoal protein
Antigenicity
Article
Bioinformatics
Cell isolation
Cell proliferation
Cytokine release
Enzyme linked immunospot assay
Human
Human cell
Humoral immunity
Ic50
Immune response
Immunofluorescence
Peripheral blood mononuclear cell
Plasmodium vivax
Western blotting
Biology
Blood
Colombia
Immunology
Metabolism
Plasmodium falciparum
Plasmodium vivax
Plasmodium vivax malaria
Cell proliferation
Colombia
Computational biology
Cytokines
Hla-drb1 chains
Humans
Immunoglobulin g
Inhibitory concentration 50
Interferon-gamma
Interleukin-10
Interleukin-6
Peptides
Plasmodium falciparum
Plasmodium vivax
Protozoan proteins
Antigenicity
Cellular and humoral response
Epitope
Hla-drb1 typing
Plasmodium vivax
Pvron2
Synthetic peptide
humoral
vivax
t-lymphocyte
protozoan
b-lymphocyte
Antigens
Epitopes
Epitopes
Immunity
Malaria
dc.subject.keyword.eng.fl_str_mv humoral
vivax
t-lymphocyte
protozoan
b-lymphocyte
Antigens
Epitopes
Epitopes
Immunity
Malaria
description Malaria caused by Plasmodium vivax is a neglected disease which is responsible for the highest morbidity in both Americas and Asia. Despite continuous public health efforts to prevent malarial infection, an effective antimalarial vaccine is still urgently needed. P. vivax vaccine development involves analyzing naturally-infected patients' immune response to the specific proteins involved in red blood cell invasion. The P. vivax rhoptry neck protein 2 (PvRON2) is a highly conserved protein which is expressed in late schizont rhoptries; it interacts directly with AMA-1 and might be involved in moving-junction formation. Bioinformatics approaches were used here to select B- and T-cell epitopes. Eleven high-affinity binding peptides were selected using the NetMHCIIpan-3.0 in silico prediction tool; their in vitro binding to HLA-DRB1*0401, HLA-DRB1*0701, HLA-DRB1*1101 or HLA-DRB1*1302 was experimentally assessed. Four peptides (39152 (HLA-DRB1*04 and 11), 39047 (HLA-DRB1*07), 39154 (HLADRB1*13) and universal peptide 39153) evoked a naturally-acquired T-cell immune response in P. vivax-exposed individuals from two endemic areas in Colombia. All four peptides had an SI greater than 2 in proliferation assays; however, only peptides 39154 and 39153 had significant differences compared to the control group. Peptide 39047 was able to significantly stimulate TNF and IL-10 production while 39154 stimulated TNF production. Allele-specific peptides (but not the universal one) were able to stimulate IL-6 production; however, none induced IFN-? production. The Bepipred 1.0 tool was used for selecting four B-cell epitopes in silico regarding humoral response. Peptide 39041 was the only one recognized by P. vivax-exposed individuals' sera and had significant differences concerning IgG subclasses; an IgG2 > IgG4 profile was observed for this peptide, agreeing with a protection-inducing role against P. falciparum and P. vivax as previously described for antigens such as RESA and MSP2. The bioinformatics results and in vitro evaluation reported here highlighted two T-cell epitopes (39047 and 39154) being recognized by memory cells and a B-cell epitope (39041) identified by P. vivax-exposed individuals' sera which could be used as potential candidates when designing a subunit-based vaccine. © 2018 López, Yepes-Pérez, Díaz-Arévalo, Patarroyo and Patarroyo.
publishDate 2018
dc.date.created.spa.fl_str_mv 2018
dc.date.accessioned.none.fl_str_mv 2020-05-26T00:08:21Z
dc.date.available.none.fl_str_mv 2020-05-26T00:08:21Z
dc.type.eng.fl_str_mv article
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dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.3389/fcimb.2018.00156
dc.identifier.issn.none.fl_str_mv 22352988
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/24079
url https://doi.org/10.3389/fcimb.2018.00156
https://repository.urosario.edu.co/handle/10336/24079
identifier_str_mv 22352988
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationIssue.none.fl_str_mv No. MAY
dc.relation.citationTitle.none.fl_str_mv Frontiers in Cellular and Infection Microbiology
dc.relation.citationVolume.none.fl_str_mv Vol. 8
dc.relation.ispartof.spa.fl_str_mv Frontiers in Cellular and Infection Microbiology, ISSN:22352988, Vol.8, No.MAY (2018)
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dc.publisher.spa.fl_str_mv Frontiers Media S.A.
institution Universidad del Rosario
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dc.source.reponame.spa.fl_str_mv reponame:Repositorio Institucional EdocUR
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spelling f04dd5c4-d685-4cee-a3d9-d2c759ef003f-1cefe4575-665d-4fc3-8e86-c6715477a2fd-110ecd4f9-843f-4ef2-bec0-7d39d3381a13-179653065-1518948826002020-05-26T00:08:21Z2020-05-26T00:08:21Z2018Malaria caused by Plasmodium vivax is a neglected disease which is responsible for the highest morbidity in both Americas and Asia. Despite continuous public health efforts to prevent malarial infection, an effective antimalarial vaccine is still urgently needed. P. vivax vaccine development involves analyzing naturally-infected patients' immune response to the specific proteins involved in red blood cell invasion. The P. vivax rhoptry neck protein 2 (PvRON2) is a highly conserved protein which is expressed in late schizont rhoptries; it interacts directly with AMA-1 and might be involved in moving-junction formation. Bioinformatics approaches were used here to select B- and T-cell epitopes. Eleven high-affinity binding peptides were selected using the NetMHCIIpan-3.0 in silico prediction tool; their in vitro binding to HLA-DRB1*0401, HLA-DRB1*0701, HLA-DRB1*1101 or HLA-DRB1*1302 was experimentally assessed. Four peptides (39152 (HLA-DRB1*04 and 11), 39047 (HLA-DRB1*07), 39154 (HLADRB1*13) and universal peptide 39153) evoked a naturally-acquired T-cell immune response in P. vivax-exposed individuals from two endemic areas in Colombia. All four peptides had an SI greater than 2 in proliferation assays; however, only peptides 39154 and 39153 had significant differences compared to the control group. Peptide 39047 was able to significantly stimulate TNF and IL-10 production while 39154 stimulated TNF production. Allele-specific peptides (but not the universal one) were able to stimulate IL-6 production; however, none induced IFN-? production. The Bepipred 1.0 tool was used for selecting four B-cell epitopes in silico regarding humoral response. Peptide 39041 was the only one recognized by P. vivax-exposed individuals' sera and had significant differences concerning IgG subclasses; an IgG2 > IgG4 profile was observed for this peptide, agreeing with a protection-inducing role against P. falciparum and P. vivax as previously described for antigens such as RESA and MSP2. The bioinformatics results and in vitro evaluation reported here highlighted two T-cell epitopes (39047 and 39154) being recognized by memory cells and a B-cell epitope (39041) identified by P. vivax-exposed individuals' sera which could be used as potential candidates when designing a subunit-based vaccine. © 2018 López, Yepes-Pérez, Díaz-Arévalo, Patarroyo and Patarroyo.application/pdfhttps://doi.org/10.3389/fcimb.2018.0015622352988https://repository.urosario.edu.co/handle/10336/24079engFrontiers Media S.A.No. MAYFrontiers in Cellular and Infection MicrobiologyVol. 8Frontiers in Cellular and Infection Microbiology, ISSN:22352988, Vol.8, No.MAY (2018)https://www.scopus.com/inward/record.uri?eid=2-s2.0-85047117634&doi=10.3389%2ffcimb.2018.00156&partnerID=40&md5=3c2d0b3ab958e4fa878817f10b199d44Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURAlkaline phosphataseEpitopeGamma interferonHla drb1 antigenInterleukin 10Interleukin 6Plasmodium vivax rhoptry neck protein 2ProteinStreptavidinTumor necrosis factorUnclassified drugCytokineEpitopeHla drb1 antigenImmunoglobulin gParasite antigenPeptideProtozoal proteinAntigenicityArticleBioinformaticsCell isolationCell proliferationCytokine releaseEnzyme linked immunospot assayHumanHuman cellHumoral immunityIc50Immune responseImmunofluorescencePeripheral blood mononuclear cellPlasmodium vivaxWestern blottingBiologyBloodColombiaImmunologyMetabolismPlasmodium falciparumPlasmodium vivaxPlasmodium vivax malariaCell proliferationColombiaComputational biologyCytokinesHla-drb1 chainsHumansImmunoglobulin gInhibitory concentration 50Interferon-gammaInterleukin-10Interleukin-6PeptidesPlasmodium falciparumPlasmodium vivaxProtozoan proteinsAntigenicityCellular and humoral responseEpitopeHla-drb1 typingPlasmodium vivaxPvron2Synthetic peptidehumoralvivaxt-lymphocyteprotozoanb-lymphocyteAntigensEpitopesEpitopesImmunityMalariaThe in vitro antigenicity of Plasmodium vivax rhoptry neck protein 2 (PvRON2) B- and T-epitopes selected by HLA-DRB1 binding profilearticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501López, CarolinaYepes-Pérez, YoelisPatarroyo, Manuel E.Patarroyo, Manuel A.Díaz Arévalo, DianaORIGINALfcimb-08-00156.pdfapplication/pdf2800525https://repository.urosario.edu.co/bitstreams/b0146dde-b4c0-4127-bd06-e4708f0f0a11/download747a819e0eb49e56fa06f402156f683fMD51TEXTfcimb-08-00156.pdf.txtfcimb-08-00156.pdf.txtExtracted texttext/plain86985https://repository.urosario.edu.co/bitstreams/b73e83b0-f7e1-4275-a1ff-4a478aa6030c/download74ea3870c466c0efc5e8a3a4f43191afMD52THUMBNAILfcimb-08-00156.pdf.jpgfcimb-08-00156.pdf.jpgGenerated Thumbnailimage/jpeg4392https://repository.urosario.edu.co/bitstreams/2ec7b7cc-6672-4237-9d5d-e658456c65f7/download2697c56833a0bed3edc0561211d3dca8MD5310336/24079oai:repository.urosario.edu.co:10336/240792022-05-02 07:37:14.910614https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co