Molecular epidemiology of entamoeba : First description of Entamoeba moshkovskii in a rural area from central Colombia

Background Entamoeba histolytica, E. dispar and E. moshkovskii are the most frequent species described in human infection where E. histolytica is the only true pathogen. The epidemiology of this infection is complex due to the absence of a routine exam that allows a correct discrimination of the Ent...

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Fecha de publicación:
2015
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/19018
Acceso en línea:
https://doi.org/10.1371/journal.pone.0140302
http://repository.urosario.edu.co/handle/10336/19018
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ARN 18C
Adolescente
Amebiasis
Infección amebiana
Infección asintomática
Niño
Estudio transversal
Extracción de ADN
Secuencia de ADN
Entamoeba Dispar
Entamoeba Dispar Infection
Infección por Entamoeba muscovensis
Análisis de heces
Humano
Estudio clínico principal
Masculino
Microscopía
Infección mixta
Epidemiología Molecular
Reacción en cadena de la polimerasa
Área rural
Clasificación
Entamoeba
Heces
Genética
Recién nacido
Parasitología
Filogenia
Niño preescolar
Predominio
Población rural
Coinfección
Incidencia & prevención de la enfermedad
Adult
Coinfection
Female
Humans
Rural Population
Prevalence
Preschool Child
Phylogeny
Parasitology
Newborn
Genetics
Classification
Feces
Rural Area
Polymerase Chain Reaction
Molecular Epidemiology
Microscopy
Mixed Infection
Major Clinical Study
Male
Human
Adolescent
Feces Analysis
Dna Sequence
Dna Extraction
Cross-Sectional Study
Child
Asymptomatic Infection
Amebic Infection
Amebiasis
Rna 18S
Entamoeba Moshkovskii Infection
Epidemiología
Infecciones
Entamoeba
Rights
License
Abierto (Texto Completo)
id EDOCUR2_1f74aaecf6948357b2bafaf62cad6119
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network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
dc.title.spa.fl_str_mv Molecular epidemiology of entamoeba : First description of Entamoeba moshkovskii in a rural area from central Colombia
title Molecular epidemiology of entamoeba : First description of Entamoeba moshkovskii in a rural area from central Colombia
spellingShingle Molecular epidemiology of entamoeba : First description of Entamoeba moshkovskii in a rural area from central Colombia
ARN 18C
Adolescente
Amebiasis
Infección amebiana
Infección asintomática
Niño
Estudio transversal
Extracción de ADN
Secuencia de ADN
Entamoeba Dispar
Entamoeba Dispar Infection
Infección por Entamoeba muscovensis
Análisis de heces
Humano
Estudio clínico principal
Masculino
Microscopía
Infección mixta
Epidemiología Molecular
Reacción en cadena de la polimerasa
Área rural
Clasificación
Entamoeba
Heces
Genética
Recién nacido
Parasitología
Filogenia
Niño preescolar
Predominio
Población rural
Coinfección
Incidencia & prevención de la enfermedad
Adult
Coinfection
Female
Humans
Rural Population
Prevalence
Preschool Child
Phylogeny
Parasitology
Newborn
Genetics
Classification
Feces
Rural Area
Polymerase Chain Reaction
Molecular Epidemiology
Microscopy
Mixed Infection
Major Clinical Study
Male
Human
Adolescent
Feces Analysis
Dna Sequence
Dna Extraction
Cross-Sectional Study
Child
Asymptomatic Infection
Amebic Infection
Amebiasis
Rna 18S
Entamoeba Moshkovskii Infection
Epidemiología
Infecciones
Entamoeba
title_short Molecular epidemiology of entamoeba : First description of Entamoeba moshkovskii in a rural area from central Colombia
title_full Molecular epidemiology of entamoeba : First description of Entamoeba moshkovskii in a rural area from central Colombia
title_fullStr Molecular epidemiology of entamoeba : First description of Entamoeba moshkovskii in a rural area from central Colombia
title_full_unstemmed Molecular epidemiology of entamoeba : First description of Entamoeba moshkovskii in a rural area from central Colombia
title_sort Molecular epidemiology of entamoeba : First description of Entamoeba moshkovskii in a rural area from central Colombia
dc.subject.spa.fl_str_mv ARN 18C
Adolescente
Amebiasis
Infección amebiana
Infección asintomática
Niño
Estudio transversal
Extracción de ADN
Secuencia de ADN
Entamoeba Dispar
Entamoeba Dispar Infection
Infección por Entamoeba muscovensis
Análisis de heces
Humano
Estudio clínico principal
Masculino
Microscopía
Infección mixta
Epidemiología Molecular
Reacción en cadena de la polimerasa
Área rural
Clasificación
Entamoeba
Heces
Genética
Recién nacido
Parasitología
Filogenia
Niño preescolar
Predominio
Población rural
Coinfección
topic ARN 18C
Adolescente
Amebiasis
Infección amebiana
Infección asintomática
Niño
Estudio transversal
Extracción de ADN
Secuencia de ADN
Entamoeba Dispar
Entamoeba Dispar Infection
Infección por Entamoeba muscovensis
Análisis de heces
Humano
Estudio clínico principal
Masculino
Microscopía
Infección mixta
Epidemiología Molecular
Reacción en cadena de la polimerasa
Área rural
Clasificación
Entamoeba
Heces
Genética
Recién nacido
Parasitología
Filogenia
Niño preescolar
Predominio
Población rural
Coinfección
Incidencia & prevención de la enfermedad
Adult
Coinfection
Female
Humans
Rural Population
Prevalence
Preschool Child
Phylogeny
Parasitology
Newborn
Genetics
Classification
Feces
Rural Area
Polymerase Chain Reaction
Molecular Epidemiology
Microscopy
Mixed Infection
Major Clinical Study
Male
Human
Adolescent
Feces Analysis
Dna Sequence
Dna Extraction
Cross-Sectional Study
Child
Asymptomatic Infection
Amebic Infection
Amebiasis
Rna 18S
Entamoeba Moshkovskii Infection
Epidemiología
Infecciones
Entamoeba
dc.subject.ddc.spa.fl_str_mv Incidencia & prevención de la enfermedad
dc.subject.keyword.eng.fl_str_mv Adult
Coinfection
Female
Humans
Rural Population
Prevalence
Preschool Child
Phylogeny
Parasitology
Newborn
Genetics
Classification
Feces
Rural Area
Polymerase Chain Reaction
Molecular Epidemiology
Microscopy
Mixed Infection
Major Clinical Study
Male
Human
Adolescent
Feces Analysis
Dna Sequence
Dna Extraction
Cross-Sectional Study
Child
Asymptomatic Infection
Amebic Infection
Amebiasis
Rna 18S
dc.subject.keyword.ben.fl_str_mv Entamoeba Moshkovskii Infection
dc.subject.lemb.spa.fl_str_mv Epidemiología
Infecciones
Entamoeba
description Background Entamoeba histolytica, E. dispar and E. moshkovskii are the most frequent species described in human infection where E. histolytica is the only true pathogen. The epidemiology of this infection is complex due to the absence of a routine exam that allows a correct discrimination of the Entamoeba species complex. Therefore, molecular methods appear as the unique epidemiological tool to accomplish the species discrimination. Herein, we conducted a cross-sectional study to determine the frequency of Entamoeba species infections in a group of asymptomatic individuals from a rural area in central Colombia. Methodology/Principal Findings A total of 181 fecal samples from asymptomatic children under 16 years old from the hamlet La Vírgen, Cundinamarca (Colombia) that voluntarily accepted to participate in the study were collected. The fecal samples were examined by light microscopy and DNA-extracted, subsequently submitted to molecular discrimination of E. dispar/E. histolytica/E. moshkovskii infection based on a multiplex PCR assay targeting the 18S rRNA fragment. To confirm the species description, twenty samples were randomly submitted to DNA sequencing of the aforementioned fragment. By direct microscopic examination, frequency of the complex E. histolytica/E. dispar/E. moshkovskii was 18.8%(34/181). PCR showed a frequency of 49.1% (89/181), discriminated as 23.2%(42/181) that were positive for E. dispar, 25.4% (46/181) for E. moshkovskii and 0.55% (1/ 181) for E. histolytica. Also, mixed infections were detected between E. dispar and E. moshkovskii at 4.42% (8/181) of the samples. Molecular barcoding confirmed the diagnosis depicted by the multiplex PCR assay. Conclusions/Significance This is the first description of E. moshkovskii in Colombia and the second report in South- America to our knowledge. Our results suggest the need to unravel the true epidemiology of Entamoeba infections around the world, including the real pathogenic role that E. moshkovskii may have. Copyright: © 2015 López et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
publishDate 2015
dc.date.created.none.fl_str_mv 2015
dc.date.issued.none.fl_str_mv 2015
dc.date.accessioned.none.fl_str_mv 2019-02-06T15:26:00Z
dc.date.available.none.fl_str_mv 2019-02-06T15:26:00Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
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dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1371/journal.pone.0140302
dc.identifier.issn.none.fl_str_mv ISSN 1932-6203
dc.identifier.uri.none.fl_str_mv http://repository.urosario.edu.co/handle/10336/19018
url https://doi.org/10.1371/journal.pone.0140302
http://repository.urosario.edu.co/handle/10336/19018
identifier_str_mv ISSN 1932-6203
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationIssue.none.fl_str_mv No. 10
dc.relation.citationTitle.none.fl_str_mv PLoS ONE
dc.relation.citationVolume.none.fl_str_mv Vol. 10
dc.relation.ispartof.spa.fl_str_mv PLoS ONE, ISSN: 1932-6203, Vol. 10/No. 10 (2015)
dc.relation.uri.spa.fl_str_mv https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0140302&type=printable
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
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dc.rights.cc.spa.fl_str_mv https://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv Abierto (Texto Completo)
https://creativecommons.org/licenses/by/4.0/
http://purl.org/coar/access_right/c_abf2
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institution Universidad del Rosario
dc.source.bibliographicCitation.spa.fl_str_mv WHO/PAHO/UNESCO report of a consultation with experts on amoebiasis (1997) Epidemiol Bull., 18, pp. 13-14
dc.source.instname.none.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.none.fl_str_mv reponame:Repositorio Institucional EdocUR
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spelling 5d6d52a3-9519-49c7-9e3e-dc3150a7ea8a6009e515a17-bef3-4b2e-91b8-1e711719314d600739d11d5-f9dc-408c-9049-179c18b58e91600432e86d0-339d-49b7-a9b1-eea86c63cc456006d1ccf89-8ea7-4398-aaa7-e0de44531a5b600903edccb-a237-40d8-bec0-b7373aafae0860010117161186002019-02-06T15:26:00Z2019-02-06T15:26:00Z20152015Background Entamoeba histolytica, E. dispar and E. moshkovskii are the most frequent species described in human infection where E. histolytica is the only true pathogen. The epidemiology of this infection is complex due to the absence of a routine exam that allows a correct discrimination of the Entamoeba species complex. Therefore, molecular methods appear as the unique epidemiological tool to accomplish the species discrimination. Herein, we conducted a cross-sectional study to determine the frequency of Entamoeba species infections in a group of asymptomatic individuals from a rural area in central Colombia. Methodology/Principal Findings A total of 181 fecal samples from asymptomatic children under 16 years old from the hamlet La Vírgen, Cundinamarca (Colombia) that voluntarily accepted to participate in the study were collected. The fecal samples were examined by light microscopy and DNA-extracted, subsequently submitted to molecular discrimination of E. dispar/E. histolytica/E. moshkovskii infection based on a multiplex PCR assay targeting the 18S rRNA fragment. To confirm the species description, twenty samples were randomly submitted to DNA sequencing of the aforementioned fragment. By direct microscopic examination, frequency of the complex E. histolytica/E. dispar/E. moshkovskii was 18.8%(34/181). PCR showed a frequency of 49.1% (89/181), discriminated as 23.2%(42/181) that were positive for E. dispar, 25.4% (46/181) for E. moshkovskii and 0.55% (1/ 181) for E. histolytica. Also, mixed infections were detected between E. dispar and E. moshkovskii at 4.42% (8/181) of the samples. Molecular barcoding confirmed the diagnosis depicted by the multiplex PCR assay. Conclusions/Significance This is the first description of E. moshkovskii in Colombia and the second report in South- America to our knowledge. Our results suggest the need to unravel the true epidemiology of Entamoeba infections around the world, including the real pathogenic role that E. moshkovskii may have. Copyright: © 2015 López et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.application/pdfhttps://doi.org/10.1371/journal.pone.0140302ISSN 1932-6203http://repository.urosario.edu.co/handle/10336/19018engNo. 10PLoS ONEVol. 10PLoS ONE, ISSN: 1932-6203, Vol. 10/No. 10 (2015)https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0140302&type=printableAbierto (Texto Completo)https://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2WHO/PAHO/UNESCO report of a consultation with experts on amoebiasis (1997) Epidemiol Bull., 18, pp. 13-14instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURARN 18CAdolescenteAmebiasisInfección amebianaInfección asintomáticaNiñoEstudio transversalExtracción de ADNSecuencia de ADNEntamoeba DisparEntamoeba Dispar InfectionInfección por Entamoeba muscovensisAnálisis de hecesHumanoEstudio clínico principalMasculinoMicroscopíaInfección mixtaEpidemiología MolecularReacción en cadena de la polimerasaÁrea ruralClasificaciónEntamoebaHecesGenéticaRecién nacidoParasitologíaFilogeniaNiño preescolarPredominioPoblación ruralCoinfecciónIncidencia & prevención de la enfermedad614600AdultCoinfectionFemaleHumansRural PopulationPrevalencePreschool ChildPhylogenyParasitologyNewbornGeneticsClassificationFecesRural AreaPolymerase Chain ReactionMolecular EpidemiologyMicroscopyMixed InfectionMajor Clinical StudyMaleHumanAdolescentFeces AnalysisDna SequenceDna ExtractionCross-Sectional StudyChildAsymptomatic InfectionAmebic InfectionAmebiasisRna 18SEntamoeba Moshkovskii InfectionEpidemiologíaInfeccionesEntamoebaMolecular epidemiology of entamoeba : First description of Entamoeba moshkovskii in a rural area from central ColombiaarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501López, Myriam ConsueloLeón, Cielo M.Fonseca, JairoReyes, PatriciaMoncada, LigiaOlivera, Mario J.Ramírez, Juan DavidLópez, Myriam ConsueloLeón, Cielo M.Fonseca, JairoReyes, PatriciaMoncada, LigiaOlivera, Mario J.Ramírez, Juan DavidORIGINAL80.pdfapplication/pdf561328https://repository.urosario.edu.co/bitstreams/71a956f1-3161-4115-bf7a-d8f6b23b7281/download2a32073b11310811629988c4fc54624dMD51TEXT80.pdf.txt80.pdf.txtExtracted texttext/plain35725https://repository.urosario.edu.co/bitstreams/08d2513f-d328-439b-b5b7-a2c287643594/downloadaaa5782b969be1185669ec27a29c994aMD52THUMBNAIL80.pdf.jpg80.pdf.jpgGenerated Thumbnailimage/jpeg4347https://repository.urosario.edu.co/bitstreams/4e46a2fa-7c9b-44d5-a408-f1dbe229f1fc/download92fe1cd6bb200a9d74ba79d8b9e56b2bMD5310336/19018oai:repository.urosario.edu.co:10336/190182022-08-27 10:45:36.206https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co