Clinical performance of a quantitative pan-genus Leishmania Real-time PCR assay for diagnosis of cutaneous and visceral leishmaniasis

Leishmaniasis is a complex vector-borne disease caused by various Leishmania species, affecting humans and animals. Current diagnostic methods have limitations, leading to potential misdiagnosis. Therefore, there is an urgent need for specific and sensitive diagnostic tools. We evaluated the sensiti...

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Tipo de recurso:
Fecha de publicación:
2023
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/42101
Acceso en línea:
https://repository.urosario.edu.co/handle/10336/42101
Palabra clave:
Cutaneous
Diagnosis
Leishmania
Monitoring
Real-time PCR
Visceral.
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License
Attribution-NonCommercial-ShareAlike 4.0 International
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dc.title.spa.fl_str_mv Clinical performance of a quantitative pan-genus Leishmania Real-time PCR assay for diagnosis of cutaneous and visceral leishmaniasis
title Clinical performance of a quantitative pan-genus Leishmania Real-time PCR assay for diagnosis of cutaneous and visceral leishmaniasis
spellingShingle Clinical performance of a quantitative pan-genus Leishmania Real-time PCR assay for diagnosis of cutaneous and visceral leishmaniasis

Cutaneous
Diagnosis
Leishmania
Monitoring
Real-time PCR
Visceral.
title_short Clinical performance of a quantitative pan-genus Leishmania Real-time PCR assay for diagnosis of cutaneous and visceral leishmaniasis
title_full Clinical performance of a quantitative pan-genus Leishmania Real-time PCR assay for diagnosis of cutaneous and visceral leishmaniasis
title_fullStr Clinical performance of a quantitative pan-genus Leishmania Real-time PCR assay for diagnosis of cutaneous and visceral leishmaniasis
title_full_unstemmed Clinical performance of a quantitative pan-genus Leishmania Real-time PCR assay for diagnosis of cutaneous and visceral leishmaniasis
title_sort Clinical performance of a quantitative pan-genus Leishmania Real-time PCR assay for diagnosis of cutaneous and visceral leishmaniasis
dc.creator.spa.fl_str_mv

author
author_facet
author_role author
dc.subject.spa.fl_str_mv Cutaneous
Diagnosis
Leishmania
Monitoring
Real-time PCR
Visceral.
topic Cutaneous
Diagnosis
Leishmania
Monitoring
Real-time PCR
Visceral.
description Leishmaniasis is a complex vector-borne disease caused by various Leishmania species, affecting humans and animals. Current diagnostic methods have limitations, leading to potential misdiagnosis. Therefore, there is an urgent need for specific and sensitive diagnostic tools. We evaluated the sensitivity of a quantitative real-time PCR (qPCR) assay targeting the 18S gene in diverse clinical sample matrices. The assay showed a wide dynamic range and a limit of detection (LoD) of 1 parasite equivalent per milliliter (eq-p/mL) for all tested species. It exhibited high specificity for Leishmania DNA, with no amplification against other microorganisms. When applied to samples from patients with visceral and cutaneous leishmaniasis, the qPCR assay provided results that matched the reference methods and allowed estimation of parasite burdens. This assay holds promise for diagnosing and monitoring leishmaniasis by offering high sensitivity, specificity, and the ability to estimate parasitemia. Further studies are needed to enhance Leishmania molecular diagnostics and expand their coverage for improved clinical impact.
publishDate 2023
dc.date.created.spa.fl_str_mv 2023-11-01
dc.date.issued.spa.fl_str_mv 2023
dc.date.accessioned.none.fl_str_mv 2024-01-31T18:22:11Z
dc.date.available.none.fl_str_mv 2024-01-31T18:22:11Z
dc.type.spa.fl_str_mv article
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dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.spa.fl_str_mv 10.1016/j.plabm.2023.e00341
dc.identifier.issn.spa.fl_str_mv 2352-5517
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/42101
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2352-5517
url https://repository.urosario.edu.co/handle/10336/42101
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.uri.spa.fl_str_mv https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10570565/pdf/main.pdf
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http://purl.org/coar/access_right/c_abf2
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dc.publisher.spa.fl_str_mv Universidad del Rosario
dc.source.spa.fl_str_mv Practical Laboratory Medicine
institution Universidad del Rosario
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dc.source.reponame.spa.fl_str_mv reponame:Repositorio Institucional EdocUR
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