Ecology of Trypanosoma cruzi I genotypes across Rhodnius prolixus captured in Attalea butyracea palms

Trypanosoma cruzi, the agent of Chagas disease exhibits significant genetic diversity. This parasite is divided into six discrete typing units (DTUs) where T. cruzi I (TcI) is the most widespread in the Americas. TcI genotypes have been associated to domestic and sylvatic cycles of transmission (TcI...

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Autores:
Tipo de recurso:
Fecha de publicación:
2017
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/24302
Acceso en línea:
https://doi.org/10.1016/j.meegid.2017.01.017
https://repository.urosario.edu.co/handle/10336/24302
Palabra clave:
Adult
Arecaceae
Article
Attalea butyracea
Controlled study
Disease control
Disease transmission
Molecular typing
Nonhuman
Principal component analysis
Rhodnius prolixus
Taxonomic identification
Trypanosoma cruzi
Trypanosoma cruzi i
Animal
Arecaceae
Chagas disease
Classification
Colombia
Ecosystem
Genetics
Genotype
Human
Insect control
Insect vector
Isolation and purification
Organization and management
Parasitology
Phylogeny
Plant leaf
Rhodnius
Transmission
Trypanosoma cruzi
Protozoal dna
Animals
Arecaceae
Chagas disease
Colombia
Ecosystem
Genotype
Humans
Insect control
Insect vectors
Molecular typing
Phylogeny
Plant leaves
Rhodnius
Trypanosoma cruzi
A
Butyracea
Chagas disease
Domestic cycle
Dtus
R
Prolixus
Sylvatic cycle
T
Cruzi
protozoan
Dna
Rights
License
Abierto (Texto Completo)
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network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
spelling d25dc61f-f5ee-4c19-95d9-f9886a857d1f5a1fe760-e03f-453c-aff4-5460450c0e280f492211-f524-4e02-8ed5-2634fcf620f010117161186002020-05-26T00:11:26Z2020-05-26T00:11:26Z2017Trypanosoma cruzi, the agent of Chagas disease exhibits significant genetic diversity. This parasite is divided into six discrete typing units (DTUs) where T. cruzi I (TcI) is the most widespread in the Americas. TcI genotypes have been associated to domestic and sylvatic cycles of transmission (TcIDom and sylvatic TcI). Due to the importance of the enzootic transmission, we determined the frequency of TcI genotypes present in Rhodnius prolixus captured in different regions of the palm A. butyracea to understand the ecology of the disease and the importance of A. butyracea palms as ecotopes of R. prolixus. Forty A. butyracea palms were sampled (base crown, mid-point and crown) capturing 105 individuals identified as R. prolixus by morphological and molecular barcoding. We conducted molecular detection and typing of T. cruzi across 59 individuals. The results showed that all the insects were infected with TcI; 28.57% were sylvatic TcI, 12.38% TcIDom and 15,24% mixed infections (TcIDom/sylvatic TcI). Statistical analysis showed a similar behavior between TcIDom and mixed infections in the mid-point and at the crown of the palm, being more frequent in the crown, while sylvatic TcI does not seem to have a specific association with any of the sampled areas. These findings are consistent with other studies showing high mobility of the insect vector between different ecotopes, increasing the need to develop improvements in the programs of disease control. © 2017 Elsevier B.V.application/pdfhttps://doi.org/10.1016/j.meegid.2017.01.01715671348https://repository.urosario.edu.co/handle/10336/24302engElsevier B.V.150146Infection, Genetics and EvolutionVol. 49Infection, Genetics and Evolution, ISSN:15671348, Vol.49,(2017); pp. 146-150https://www.scopus.com/inward/record.uri?eid=2-s2.0-85009865299&doi=10.1016%2fj.meegid.2017.01.017&partnerID=40&md5=a167640bcdec098644777e712f5e49d5Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURAdultArecaceaeArticleAttalea butyraceaControlled studyDisease controlDisease transmissionMolecular typingNonhumanPrincipal component analysisRhodnius prolixusTaxonomic identificationTrypanosoma cruziTrypanosoma cruzi iAnimalArecaceaeChagas diseaseClassificationColombiaEcosystemGeneticsGenotypeHumanInsect controlInsect vectorIsolation and purificationOrganization and managementParasitologyPhylogenyPlant leafRhodniusTransmissionTrypanosoma cruziProtozoal dnaAnimalsArecaceaeChagas diseaseColombiaEcosystemGenotypeHumansInsect controlInsect vectorsMolecular typingPhylogenyPlant leavesRhodniusTrypanosoma cruziAButyraceaChagas diseaseDomestic cycleDtusRProlixusSylvatic cycleTCruziprotozoanDnaEcology of Trypanosoma cruzi I genotypes across Rhodnius prolixus captured in Attalea butyracea palmsarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Poveda C.Higuera A.Urbano P.Ramírez, Juan David10336/24302oai:repository.urosario.edu.co:10336/243022022-05-02 07:37:16.473972https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Ecology of Trypanosoma cruzi I genotypes across Rhodnius prolixus captured in Attalea butyracea palms
title Ecology of Trypanosoma cruzi I genotypes across Rhodnius prolixus captured in Attalea butyracea palms
spellingShingle Ecology of Trypanosoma cruzi I genotypes across Rhodnius prolixus captured in Attalea butyracea palms
Adult
Arecaceae
Article
Attalea butyracea
Controlled study
Disease control
Disease transmission
Molecular typing
Nonhuman
Principal component analysis
Rhodnius prolixus
Taxonomic identification
Trypanosoma cruzi
Trypanosoma cruzi i
Animal
Arecaceae
Chagas disease
Classification
Colombia
Ecosystem
Genetics
Genotype
Human
Insect control
Insect vector
Isolation and purification
Organization and management
Parasitology
Phylogeny
Plant leaf
Rhodnius
Transmission
Trypanosoma cruzi
Protozoal dna
Animals
Arecaceae
Chagas disease
Colombia
Ecosystem
Genotype
Humans
Insect control
Insect vectors
Molecular typing
Phylogeny
Plant leaves
Rhodnius
Trypanosoma cruzi
A
Butyracea
Chagas disease
Domestic cycle
Dtus
R
Prolixus
Sylvatic cycle
T
Cruzi
protozoan
Dna
title_short Ecology of Trypanosoma cruzi I genotypes across Rhodnius prolixus captured in Attalea butyracea palms
title_full Ecology of Trypanosoma cruzi I genotypes across Rhodnius prolixus captured in Attalea butyracea palms
title_fullStr Ecology of Trypanosoma cruzi I genotypes across Rhodnius prolixus captured in Attalea butyracea palms
title_full_unstemmed Ecology of Trypanosoma cruzi I genotypes across Rhodnius prolixus captured in Attalea butyracea palms
title_sort Ecology of Trypanosoma cruzi I genotypes across Rhodnius prolixus captured in Attalea butyracea palms
dc.subject.keyword.spa.fl_str_mv Adult
Arecaceae
Article
Attalea butyracea
Controlled study
Disease control
Disease transmission
Molecular typing
Nonhuman
Principal component analysis
Rhodnius prolixus
Taxonomic identification
Trypanosoma cruzi
Trypanosoma cruzi i
Animal
Arecaceae
Chagas disease
Classification
Colombia
Ecosystem
Genetics
Genotype
Human
Insect control
Insect vector
Isolation and purification
Organization and management
Parasitology
Phylogeny
Plant leaf
Rhodnius
Transmission
Trypanosoma cruzi
Protozoal dna
Animals
Arecaceae
Chagas disease
Colombia
Ecosystem
Genotype
Humans
Insect control
Insect vectors
Molecular typing
Phylogeny
Plant leaves
Rhodnius
Trypanosoma cruzi
A
Butyracea
Chagas disease
Domestic cycle
Dtus
R
Prolixus
Sylvatic cycle
T
Cruzi
topic Adult
Arecaceae
Article
Attalea butyracea
Controlled study
Disease control
Disease transmission
Molecular typing
Nonhuman
Principal component analysis
Rhodnius prolixus
Taxonomic identification
Trypanosoma cruzi
Trypanosoma cruzi i
Animal
Arecaceae
Chagas disease
Classification
Colombia
Ecosystem
Genetics
Genotype
Human
Insect control
Insect vector
Isolation and purification
Organization and management
Parasitology
Phylogeny
Plant leaf
Rhodnius
Transmission
Trypanosoma cruzi
Protozoal dna
Animals
Arecaceae
Chagas disease
Colombia
Ecosystem
Genotype
Humans
Insect control
Insect vectors
Molecular typing
Phylogeny
Plant leaves
Rhodnius
Trypanosoma cruzi
A
Butyracea
Chagas disease
Domestic cycle
Dtus
R
Prolixus
Sylvatic cycle
T
Cruzi
protozoan
Dna
dc.subject.keyword.eng.fl_str_mv protozoan
Dna
description Trypanosoma cruzi, the agent of Chagas disease exhibits significant genetic diversity. This parasite is divided into six discrete typing units (DTUs) where T. cruzi I (TcI) is the most widespread in the Americas. TcI genotypes have been associated to domestic and sylvatic cycles of transmission (TcIDom and sylvatic TcI). Due to the importance of the enzootic transmission, we determined the frequency of TcI genotypes present in Rhodnius prolixus captured in different regions of the palm A. butyracea to understand the ecology of the disease and the importance of A. butyracea palms as ecotopes of R. prolixus. Forty A. butyracea palms were sampled (base crown, mid-point and crown) capturing 105 individuals identified as R. prolixus by morphological and molecular barcoding. We conducted molecular detection and typing of T. cruzi across 59 individuals. The results showed that all the insects were infected with TcI; 28.57% were sylvatic TcI, 12.38% TcIDom and 15,24% mixed infections (TcIDom/sylvatic TcI). Statistical analysis showed a similar behavior between TcIDom and mixed infections in the mid-point and at the crown of the palm, being more frequent in the crown, while sylvatic TcI does not seem to have a specific association with any of the sampled areas. These findings are consistent with other studies showing high mobility of the insect vector between different ecotopes, increasing the need to develop improvements in the programs of disease control. © 2017 Elsevier B.V.
publishDate 2017
dc.date.created.spa.fl_str_mv 2017
dc.date.accessioned.none.fl_str_mv 2020-05-26T00:11:26Z
dc.date.available.none.fl_str_mv 2020-05-26T00:11:26Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1016/j.meegid.2017.01.017
dc.identifier.issn.none.fl_str_mv 15671348
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/24302
url https://doi.org/10.1016/j.meegid.2017.01.017
https://repository.urosario.edu.co/handle/10336/24302
identifier_str_mv 15671348
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 150
dc.relation.citationStartPage.none.fl_str_mv 146
dc.relation.citationTitle.none.fl_str_mv Infection, Genetics and Evolution
dc.relation.citationVolume.none.fl_str_mv Vol. 49
dc.relation.ispartof.spa.fl_str_mv Infection, Genetics and Evolution, ISSN:15671348, Vol.49,(2017); pp. 146-150
dc.relation.uri.spa.fl_str_mv https://www.scopus.com/inward/record.uri?eid=2-s2.0-85009865299&doi=10.1016%2fj.meegid.2017.01.017&partnerID=40&md5=a167640bcdec098644777e712f5e49d5
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
rights_invalid_str_mv Abierto (Texto Completo)
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Elsevier B.V.
institution Universidad del Rosario
dc.source.instname.spa.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.spa.fl_str_mv reponame:Repositorio Institucional EdocUR
repository.name.fl_str_mv Repositorio institucional EdocUR
repository.mail.fl_str_mv edocur@urosario.edu.co
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